Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,575,695	G→A	N103N (AAC→AAT)	unknown ←	Copper‑sensing two‑component system response regulator CpxR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,575,695	0	G	A	100.0%	201.2 / NA	71	N103N (AAC→AAT)	unknown	Copper‑sensing two‑component system response regulator CpxR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/41);  total (30/41)

AGACTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCGTTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATCTAATAAGATGAGATCATAACTATGGTCAAGCAATTCCAATGCTTCTTTACCATTGTTTGCGACATTCACCTGAAAGCCTTCTAAAT  >  1/1575459‑1575939                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     aGACTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCgg                                                                                                                                                                                                                                        >  1:136807/1‑251 (MQ=255)   aCTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCggt                                                                                                                                                                                                                                       <  1:267110/250‑1 (MQ=255)      tAACTCTTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCggtaggt                                                                                                                                                                                                                                   <  1:394462/251‑1 (MQ=255)         ctctTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAgt                                                                                                                                                                                                                                <  1:654947/251‑1 (MQ=255)         ctctTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAgt                                                                                                                                                                                                                                <  1:495157/251‑1 (MQ=255)           ctTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAgtcg                                                                                                                                                                                                                              >  1:328455/1‑251 (MQ=255)           ctTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAgtcg                                                                                                                                                                                                                              <  1:364834/251‑1 (MQ=255)           ctTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAgtcg                                                                                                                                                                                                                              >  1:665115/1‑251 (MQ=255)             tCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAgtcgtc                                                                                                                                                                                                                            <  1:434456/251‑1 (MQ=255)                  aGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAgt                                                                                                                                                                                                                                <  1:96210/242‑1 (MQ=255)                  aGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCa                                                                                                                                                                                                                        >  1:312046/1‑250 (MQ=255)                    tCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCAcc                                                                                                                                                                                                                      <  1:389597/250‑1 (MQ=255)                       gAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAAc                                                                                                                                                                                                                                                <  1:508760/221‑1 (MQ=255)                       gAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAAt                                                                                                                                                                                                                  >  1:750051/1‑251 (MQ=255)                         aTTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTc                                                                                                                                                                                                                <  1:238705/251‑1 (MQ=255)                         aTTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTc                                                                                                                                                                                                                <  1:173472/251‑1 (MQ=255)                            aaCCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGAGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGCCGGATCTTTCGCGCTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAg                                                                                                                                                                                                             >  1:506132/1‑251 (MQ=255)                                     tGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAAt                                                                                                                                                                                                    <  1:60732/251‑1 (MQ=255)                                      gAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATa                                                                                                                                                                                                   >  1:301670/1‑251 (MQ=255)                                        gTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAAc                                                                                                                                                                                                                                                <  1:818644/204‑1 (MQ=255)                                        gTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCggt                                                                                                                                                                                                                                       <  1:364774/213‑1 (MQ=255)                                        gTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCActtattttcacgctcagtgtta                                                                                                                                                                                                  >  1:58224/1‑229 (MQ=255)                                        gTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCa                                                                                                                                                                                                               <  1:253722/237‑1 (MQ=255)                                             aaGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAgtcgtc                                                                                                                                                                                                                            >  1:208840/1‑219 (MQ=255)                                                     gTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCGACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATAtcatca                                                                                                                                                                                    >  1:437630/1‑251 (MQ=255)                                                      taataaAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCAc                                                                                                                                                                                   <  1:886603/251‑1 (MQ=255)                                                      taataaAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCAc                                                                                                                                                                                   <  1:768348/251‑1 (MQ=255)                                                      taataaAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCAc                                                                                                                                                                                   <  1:680082/251‑1 (MQ=255)                                                      taataaAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCAc                                                                                                                                                                                   <  1:18077/251‑1 (MQ=255)                                                        ataaAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACcg                                                                                                                                                                                 <  1:62895/251‑1 (MQ=255)                                                                  cTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCa                                                                                                                                                                       >  1:430832/1‑251 (MQ=255)                                                                      gTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACcg                                                                                                                                                                                 >  1:555025/1‑237 (MQ=255)                                                                       tGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGcatc                                                                                                                                                                  <  1:472634/251‑1 (MQ=255)                                                                             tGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGAc                                                                                                                                                             <  1:528904/250‑1 (MQ=255)                                                                                gTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCgg                                                                                                                                                          >  1:282057/1‑250 (MQ=255)                                                                                         cTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACg                                                                                                                                                                                                                                               >  1:893321/1‑156 (MQ=255)                                                                                           tttGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTGATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAg                                                                                                                                                                          <  1:698061/223‑1 (MQ=255)                                                                                            ttGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGttt                                                                                                                                             <  1:330931/251‑1 (MQ=255)                                                                                                   cAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGa                                                                                                                                      >  1:218146/1‑251 (MQ=255)                                                                                                     gCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTACCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAAt                                                                                                                                                  >  1:47746/1‑237 (MQ=255)                                                                                                            cTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAGGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGa                                                                                                                                                              >  1:447204/1‑218 (MQ=255)                                                                                                            cTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTa                                                                                                                             >  1:166941/1‑251 (MQ=255)                                                                                                                         ggATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGAc                                                                                                                                                                                                           >  1:113072/1‑160 (MQ=255)                                                                                                                          gATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCg                                                                                                               >  1:161159/1‑251 (MQ=255)                                                                                                                                         cgccgAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGcat                                                                                                 >  1:814278/1‑250 (MQ=255)                                                                                                                                                cTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAatat                                                                                         <  1:825662/251‑1 (MQ=255)                                                                                                                                                 tCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATc                                                                                        <  1:626127/251‑1 (MQ=255)                                                                                                                                                      tttATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATCtaata                                                                                   <  1:314004/251‑1 (MQ=255)                                                                                                                                                           ccACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATTATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATCTAATAAGATg                                                                              <  1:430727/251‑1 (MQ=255)                                                                                                                                                           ccACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATCTAATAAGATg                                                                              <  1:73406/251‑1 (MQ=255)                                                                                                                                                              caTCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGAc                                                                                                       >  1:235300/1‑223 (MQ=255)                                                                                                                                                                     cGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATCTAATAAGATGAGATCATAAc                                                                    <  1:30963/251‑1 (MQ=255)                                                                                                                                                                          cTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATCTAATAAGATGAGATCATAACTATgg                                                               <  1:676823/251‑1 (MQ=255)                                                                                                                                                                                  ttATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGAc                                                                                                       <  1:586491/203‑1 (MQ=255)                                                                                                                                                                                  ttATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATCTAATAAGATGAGATCATAACTATgg                                                               >  1:771212/1‑243 (MQ=255)                                                                                                                                                                                      ttGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGAc                                                                                                       <  1:625025/199‑1 (MQ=255)                                                                                                                                                                                       tGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATCTAATAAGATGAGATCATAACTATGGTCAAGCAATTCCa                                                  >  1:107450/1‑251 (MQ=255)                                                                                                                                                                                            gtgCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGGCCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATCTAATAAGATGAGATCATAACTATGGTCAAGCAATTCCAATg                                               >  1:564487/1‑249 (MQ=255)                                                                                                                                                                                            gtgCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATCTAATAAGATGAGATCATAAc                                                                    <  1:683555/228‑1 (MQ=255)                                                                                                                                                                                            gtgCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATCTAATAAGATGAGATCATAACTa                                                                  <  1:352911/230‑1 (MQ=255)                                                                                                                                                                                            gtgCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATCTAATAAGATGAGATCATAACTATGGTCAAGCAATTCCaa                                                 >  1:449909/1‑247 (MQ=255)                                                                                                                                                                                            gtgCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATCTAATAAGATGAGATCATAACTATGGTCAAGCAATTCCAATg                                               >  1:50395/1‑249 (MQ=255)                                                                                                                                                                                            gtgCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATCTAATAAGATGAGATCATAACTATGGTCAAGCAATTCCAATGc                                              <  1:255027/250‑1 (MQ=255)                                                                                                                                                                                             tgCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATCTAATAAGATGAGATCATAACTATGGTCAAGCAATTCCAATGct                                             <  1:276450/250‑1 (MQ=255)                                                                                                                                                                                                 gTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATAc                                                                                                                 >  1:784600/1‑178 (MQ=255)                                                                                                                                                                                                      tCTTAAAATCGCTTTAATTCGCGCAACCAATTCGCGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATCTAATAAGATGAGATCATAACTATgg                                                               <  1:264056/223‑1 (MQ=255)                                                                                                                                                                                                      tCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCataa                                                                                            >  1:301643/1‑194 (MQ=255)                                                                                                                                                                                                                               aCCAATTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATCTAATAAGATGAGATCATAACTATGGTCAAGCAATTCCAATGCTTCTTTACCATTGTTTGCGACATTCACCTGAAAGc           >  1:1797/1‑250 (MQ=255)                                                                                                                                                                                                                                   aTTCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATCtaat                                                                                    <  1:32971/173‑1 (MQ=255)                                                                                                                                                                                                                                     tCACGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATCTAATAAGATGAGATCATAACTATGGTCAAGCAATTCCAATGCTTCTTTACCATTGTTTGCGACATTCACCTGAAAGCCTTCTaa    <  1:859867/251‑1 (MQ=255)                                                                                                                                                                                                                                       aCGATCATTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATCTAATAAGATGAGATCATAACTATGGTCAAGCAATTCCAATGCTTCTTTACCATTGTTTGCGACATTCACCTGAAAGCCTTCTAAAt  <  1:322526/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     AGACTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTCCCGGATAAAATACGATGCCGCCGAACTCGATTTTATCCACATCTGTCGGATCTTTCGCGTTATTTGCCTGTGCTGTACGTCTTAAAATCGCTTTAATTCGCGCAACCAATTCACGATCGTTAAACGGCTTCGGTAGGTAGTCGTCCGCACCTAATTCAAGACCTAAAATACGATCAATATCATCACCGCGGGCAGTCAGCATCATGACCGGCGTGGAATAGTTTTTTCTGATTTGTTTTAATGTTTCGATACCGTTTAAGACCGGCATCATAATATCTAATAAGATGAGATCATAACTATGGTCAAGCAATTCCAATGCTTCTTTACCATTGTTTGCGACATTCACCTGAAAGCCTTCTAAAT  >  1/1575459‑1575939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑