Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,575,334 C→T G224S (GGT→AGT)  unknown ← Copper‑sensing two‑component system response regulator CpxR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,575,3340CT100.0% 104.5 / NA 38G224S (GGT→AGT) unknownCopper‑sensing two‑component system response regulator CpxR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/19);  total (19/19)

TTTTGATACTTTATCATTTCATGCTCTTTTAAATCCGAGTAAATATGCGCATCAAAATAAGGTAATAAAAAAATAAAGATTAGTAAAATGGAAAATGTGAGCCAGAAGAAAGTGAAAATTCGTATGGTGAGTGAGTTAAAAATAAATTCTTTTCTGAACATGATAATTCGGTGTGAAAAAGGCTATATAAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACCGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTC  >  1/1575103‑1575576
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  
ttttGATACTTTATCATTTCATGCTCTTTTAAATCCGAGTAAATATGCGCATCAAAATAAGGTAATAAAAAAATAAAGATTAGTAAAATGGAAAATGTGAGCCAGAAGAAAGTGAAAATTCGTATGGTGAGTGAGTTAAAAATAAATTCTTTTCTGAACATGATAATTCGGTGTGAAAAAGGCTATATAAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTa                                                                                                                                                                                                                                 >  1:214686/1‑251 (MQ=255)
                     tGCTCTTTTAAATCCGAGTAAATATGCGCATCAAAATAAGGTAATAAAAAAATAAAGATTAGTAAAATGGAAAATGTGAGCCAGAAGAAAGTGAAAATTCGTATGGTGAGTGAGTTAAAAATAAATTCTTTTCTGAACATGATAATTCGGTGTGAAAAAGGCTATATAAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGgag                                                                                                                                                                                                            <  1:121538/251‑1 (MQ=255)
                                    gAGTAAATATGCGCATCAAAATAAGGTAATAAAAAAATAAAGATTAGTAAAATGGAAAATGTGAGCCAGAAGAAAGTGAAAATTCGTATGGTGAGTGAGTTAAAAATAAATTCTTTTCTGAACATGATAATTCGGTGTGAAAAAGGCTATATAAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAAtttt                                                                                                                                                                                             >  1:52323/1‑251 (MQ=255)
                                                        aTAAGGTAATAAAAAAATAAAGATTAGTAAAATGGAAAATGTGAGCCAGAAGAAAGTGAAAATTCGTATGGTGAGTGAGTTAAAAATAAATTCTTTTCTGAACATGATAATTCGGTGTGAAAAAGGCTATATAAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATg                                                                                                                                                                         <  1:885110/251‑1 (MQ=255)
                                                              taataaAAAAATAAAGATTAGTAAAATGGAAAATGTGAGCCAGAAGAAAGTGAAAATTCGTATGGTGAGTGAGTTAAAAATAAATTCTTTTCTGAACATGATAATTCGGTGTGAAAAAGGCTATATAAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATc                                                                                                                                                                   <  1:676483/251‑1 (MQ=255)
                                                                             gATTAGTAAAATGGAAAATGTGAGCCAGAAGAAAGTGAAAATTCGTATGGTGAGTGAGTTAAAAATAAATTCTTTTCTGAACATGATAATTCGGTGTGAAAAAGGCTATATAAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGat                                                                                                                                                                                                                    <  1:750929/187‑1 (MQ=255)
                                                                                                   aGCCAGAAGAAAGTGAAAATTCGTATGGTGAGTGAGTTAAAAATAAATTCTTTTCTGAACATGATAATTCGGTGTGAAAAAGGCTATATAAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCgg                                                                                                                                                                                                              <  1:856940/171‑1 (MQ=255)
                                                                                                   aGCCAGAAGAAAGTGAAAATTCGTATGGTGAGTGAGTTAAAAATAAATTCTTTTCTGAACATGATAATTCGGTGTGAAAAAGGCTATATAAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCaaa                                                                                                                              >  1:180074/1‑251 (MQ=255)
                                                                                                                    aaTTCGTATGGTGAGTGAGTTAAAAATAAATTCTTTTCTGAACATGATAATTCGGTGTGAAAAAGGCTATATAAAAATAGCTTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAActc                                                                                                             <  1:433514/251‑1 (MQ=255)
                                                                                                                      ttCGTATGGTGAGTGAGTTAAAAATAAATTCTTTTCTGAACATGATAATTCGGTGTGAAAAAGGCTATATAAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCatat                                                                                                                                                                    >  1:600360/1‑194 (MQ=255)
                                                                                                                                      gtTAAAAATAAATTCTTTTCTGAACATGATAATTCGGTGTGAAAAAGGCTATATAAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAAt                                                                                              >  1:528408/1‑248 (MQ=255)
                                                                                                                                      gtTAAAAATAAATTCTTTTCTGAACATGATAATTCGGTGTGAAAAAGGCTATATAAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAATTaa                                                                                           >  1:633723/1‑251 (MQ=255)
                                                                                                                                        tAAAAATAAATTCTTTTCTGAACATGATAATTCGGTGTGAAAAAGGCTATATAAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAATTAAcc                                                                                         <  1:463636/251‑1 (MQ=255)
                                                                                                                                                           gAACATGATAATTCGGTGTGAAAAAGGCTATATAAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTg                                                                                                                                         >  1:385341/1‑184 (MQ=255)
                                                                                                                                                                tGATAATTCGGTGTGAAAAAGGCTATATAAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAActc                                                                                                             >  1:337971/1‑207 (MQ=255)
                                                                                                                                                                  aTAATTCGGTGTGAAAAAGGCTATATAAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGtaa                                                                <  1:674273/250‑1 (MQ=255)
                                                                                                                                                                                   aGGCTATATAAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATc                                                                                                                                                                   <  1:476010/134‑1 (MQ=255)
                                                                                                                                                                                        atataAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGAc                                                                                                                    >  1:493349/1‑176 (MQ=255)
                                                                                                                                                                                        atataAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAAc                                                                                                               <  1:857574/181‑1 (MQ=255)
                                                                                                                                                                                        atataAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAATTAAcc                                                                                         <  1:837119/203‑1 (MQ=255)
                                                                                                                                                                                        atataAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAGtaa                                                                             <  1:677960/215‑1 (MQ=255)
                                                                                                                                                                                        atataAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAGTAATAAg                                                                         <  1:308111/219‑1 (MQ=255)
                                                                                                                                                                                        atataAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGt                                                                        >  1:658556/1‑220 (MQ=255)
                                                                                                                                                                                            aaaaaTAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAg                                                                                >  1:700492/1‑208 (MQ=255)
                                                                                                                                                                                             aaaaTAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCa                                    >  1:206004/1‑251 (MQ=255)
                                                                                                                                                                                                 tAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTACGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGAt                                <  1:487727/251‑1 (MQ=255)
                                                                                                                                                                                                    ccTTTATTGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTc                                                    <  1:475411/228‑1 (MQ=255)
                                                                                                                                                                                                          ttGTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAAc                       <  1:352009/251‑1 (MQ=255)
                                                                                                                                                                                                            gTTATTTTTCCGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAAcc                      >  1:372847/1‑250 (MQ=255)
                                                                                                                                                                                                                     ccGCTATTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGcc            <  1:870444/251‑1 (MQ=255)
                                                                                                                                                                                                                          aTTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCg                                                                                                                                                      >  1:158658/1‑108 (MQ=255)
                                                                                                                                                                                                                          aTTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAgg                                                                                                                                                 >  1:534215/1‑113 (MQ=255)
                                                                                                                                                                                                                          aTTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAAt                                                                                              <  1:231301/164‑1 (MQ=255)
                                                                                                                                                                                                                          aTTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGAt                                >  1:727092/1‑226 (MQ=255)
                                                                                                                                                                                                                          aTTAATAAATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTg       >  1:323622/1‑251 (MQ=255)
                                                                                                                                                                                                                            taataaATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGt                                                                        <  1:332811/184‑1 (MQ=255)
                                                                                                                                                                                                                            taataaATAACTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTgg      >  1:91933/1‑250 (MQ=255)
                                                                                                                                                                                                                                aaataaCTGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTc  >  1:358977/1‑250 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  
TTTTGATACTTTATCATTTCATGCTCTTTTAAATCCGAGTAAATATGCGCATCAAAATAAGGTAATAAAAAAATAAAGATTAGTAAAATGGAAAATGTGAGCCAGAAGAAAGTGAAAATTCGTATGGTGAGTGAGTTAAAAATAAATTCTTTTCTGAACATGATAATTCGGTGTGAAAAAGGCTATATAAAAATAGCCTTTATTGTTATTTTTCCGCTATTAATAAATAACCGCGACCACGAAGTGTTTTAAACCATGGTAGATCATCGGAGCGAGCCGGTAATTTTTTTCGTAAATTCGACATATGCATATCAATGGCACGATCGAAAGGCGTAAGTGGCTTGCCCAAAACTTCAAGACTTAACTCTTCACGAGTCAGAATTAACCCGGGATGAGTAATAAGTTTTAGTAATAAAGCAAACTCGGTGCCGGTGAGTTCAAGATCTTTTGAACCCAGCATTGCCTGTTGGCGTC  >  1/1575103‑1575576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑