Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,567,921	T→C	H37R (CAC→CGC)	unknown ←	Tetracenomycin polyketide synthesis O‑methyltransferase TcmP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,567,921	0	T	C	100.0%	225.5 / NA	81	H37R (CAC→CGC)	unknown	Tetracenomycin polyketide synthesis O‑methyltransferase TcmP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/41);  total (40/41)

CGGCAAGCAAAATTTCATTTTCCGCCGGCGGCATCAGTTGCTTGCGCAATGCGATCACATCGGGCAAATCCAGCTCATAGAACGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGTGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTAGAGAGTCATGTGTGGCGGACAGTACGGCGCAAGCTGCCGTCGGGAAA  >  1/1567688‑1568165                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     cgGCAAGCAAAATTTCATTTTCCGCCGGCGGCATCAGTTGCTTGCGCAATGCGATCACATCGGGCAAATCCAGCTCATAGAACGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAAc                                                                                                                                                                                                                                     <  1:581893/251‑1 (MQ=255)            aTTTCATTTTCCGCCGGCGGCATCAGTTGCTTGCGCAATGCGATCACATCGGGCAAATCCAGCTCATAGAACGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATc                                                                                                                                                                                                                          <  1:882555/251‑1 (MQ=255)                                cATCAGTTGCTTGCGCAATGCGATCACATCGGGCAAATCCAGCTCATAGAACGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAg                                                                                                                                                                                                                                             <  1:475237/212‑1 (MQ=255)                                  tCAGTTGCTTGCGCAATGCGATCACATCGGGCAAATCCAGCTCATAGAACGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAgg                                                                                                                                                                                                    >  1:150595/1‑251 (MQ=255)                                      ttgcttgcGCAATGCGATCACATCGGGCAAATCCAGCTCATAGAACGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGc                                                                                                                                                                                                   <  1:803675/248‑1 (MQ=255)                                       tgcttgcGCAATGCGATCACATCGGGCAAATCCAGCTCATAGAACGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAg                                                                                                                                                                                               >  1:826935/1‑251 (MQ=255)                                       tgcttgcGCAATGCGATCACATCGGGCAAATCCAGCTCATAGAACGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAg                                                                                                                                                                                               >  1:143326/1‑251 (MQ=255)                                          ttgcGCAATGCGATCACATCGGGCAAATCCAGCTCATAGAACGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAgcgc                                                                                                                                                                                            <  1:743000/251‑1 (MQ=255)                                           tgcGCAATGCGATCACATCGGGCAAATCCAGCTCATAGAACGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGc                                                                                                                                                                                                                                               >  1:644965/1‑199 (MQ=255)                                                 aTGCGATCACATCGGGCAAATCCAGCTCATAGAACGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGcg                                                                                                                                                                                     >  1:724967/1‑251 (MQ=255)                                                     gATCACATCGGGCAAATCCAGCTCATAGAACGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATg                                                                                                                                                                                       >  1:868031/1‑245 (MQ=255)                                                     gATCACATCGGGCAAATCCAGCTCATAGAACGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGcgtcgt                                                                                                                                                                                 >  1:293683/1‑251 (MQ=255)                                                         acaTCGGGCAAATCCAGCTCATAGAACGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGaat                                                                                                                                                                             <  1:283331/251‑1 (MQ=255)                                                         acaTCGGGCAAATCCAGCTCATAGAACGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTACCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGaat                                                                                                                                                                             <  1:805835/251‑1 (MQ=255)                                                               ggCAAATCCAGCTCATAGAACGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAatcg                                                                                                                                                                        <  1:778697/250‑1 (MQ=255)                                                                         gCTCATAGAACGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTg                                                                                                                                                             >  1:337641/1‑251 (MQ=255)                                                                         gCTCATAGAACGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGCGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTg                                                                                                                                                             >  1:298125/1‑251 (MQ=255)                                                                            cATAGAACGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAgg                                                                                                                                                          <  1:868623/251‑1 (MQ=255)                                                                                gAACGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGa                                                                                                                                                        >  1:505733/1‑249 (MQ=255)                                                                                 aaCGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGAc                                                                                                                                                       <  1:243632/249‑1 (MQ=255)                                                                                     gCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGa                                                                                                                                                 >  1:602654/1‑251 (MQ=255)                                                                                         aTTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGA‑CCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAgcg                                                                                                                                            <  1:351177/251‑1 (MQ=255)                                                                                          tttttGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAgcg                                                                                                                                            <  1:606766/251‑1 (MQ=255)                                                                                                 cGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATag                                                                                                                                     <  1:482377/251‑1 (MQ=255)                                                                                                 cGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATag                                                                                                                                     <  1:566859/251‑1 (MQ=255)                                                                                                     gTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATag                                                                                                                                     >  1:263725/1‑247 (MQ=255)                                                                                                         cccACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAAt                                                                                                                             >  1:690840/1‑251 (MQ=255)                                                                                                               cgcTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCa                                                                                                                        <  1:519597/250‑1 (MQ=255)                                                                                                               cgcTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAg                                                                                                                       <  1:794642/251‑1 (MQ=255)                                                                                                                 cTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAatcggat                                                                                                                                                                     <  1:536825/203‑1 (MQ=255)                                                                                                                 cTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGAt                                                                                                                                                <  1:232902/224‑1 (MQ=255)                                                                                                                 cTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAggg                                                                                                                     >  1:349049/1‑251 (MQ=255)                                                                                                                 cTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAggg                                                                                                                     >  1:856303/1‑251 (MQ=255)                                                                                                                 cTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAggg                                                                                                                     >  1:269689/1‑251 (MQ=255)                                                                                                                       aCGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATag                                                                                                                                     >  1:194339/1‑229 (MQ=255)                                                                                                                          cgcATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATGGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGa                                                                                                            >  1:843688/1‑251 (MQ=255)                                                                                                                            cATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGAc                                                                                                           >  1:849102/1‑250 (MQ=255)                                                                                                                                  gCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATagag                                                                                                                                   <  1:890326/220‑1 (MQ=255)                                                                                                                                         aCCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCa                                                                                                                        <  1:480667/224‑1 (MQ=255)                                                                                                                                            gATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGaat                                                                                                                                                                             <  1:332458/168‑1 (MQ=255)                                                                                                                                             atcatcACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAAc                                                                                         <  1:126743/251‑1 (MQ=255)                                                                                                                                                      aTAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGTGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTAc                                                                                 >  1:671200/1‑250 (MQ=255)                                                                                                                                                      aTAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTAc                                                                                 <  1:304430/250‑1 (MQ=255)                                                                                                                                                      aTAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTAc                                                                                 <  1:575488/250‑1 (MQ=255)                                                                                                                                                        aaTCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCatat                                                                                                                                       >  1:389689/1‑194 (MQ=255)                                                                                                                                                           cGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGaat                                                                                                                                                                             >  1:120658/1‑153 (MQ=255)                                                                                                                                                            ggTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTAcctc                                                                              <  1:359947/247‑1 (MQ=255)                                                                                                                                                             gttgttGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAatcggat                                                                                                                                                                     >  1:508246/1‑159 (MQ=255)                                                                                                                                                             gttgttGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGc                                                                                                                         <  1:245729/203‑1 (MQ=255)                                                                                                                                                               tgttgGTGCTGTTGAATAAAGGCTGTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTgg                                                                       <  1:646238/251‑1 (MQ=255)                                                                                                                                                                    gTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTAcc                                                                                <  1:800764/237‑1 (MQ=255)                                                                                                                                                                    gTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAAcg                                                                  >  1:130613/1‑251 (MQ=255)                                                                                                                                                                               tAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAAc                                                       <  1:230920/251‑1 (MQ=255)                                                                                                                                                                                         ggTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTagaga                                             >  1:689648/1‑251 (MQ=255)                                                                                                                                                                                          gTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTagaga                                             <  1:439156/250‑1 (MQ=255)                                                                                                                                                                                             aGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCatat                                                                                                                                       <  1:436040/157‑1 (MQ=255)                                                                                                                                                                                                  aTCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAg                                                                                                                       <  1:556826/168‑1 (MQ=255)                                                                                                                                                                                                  aTCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTAGAGAGTCAtgtgt                                    >  1:34446/1‑251 (MQ=255)                                                                                                                                                                                                  aTCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGATCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTAGAGAGTCAtgtgt                                    >  1:442684/1‑251 (MQ=255)                                                                                                                                                                                                    ccAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAgg                                                                                                                                                          >  1:185113/1‑131 (MQ=255)                                                                                                                                                                                                    ccAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTAGAGAGTCATGTGTgg                                  >  1:120054/1‑251 (MQ=255)                                                                                                                                                                                                     cAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGAt                                                                                                                                                <  1:343420/140‑1 (MQ=255)                                                                                                                                                                                                     cAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATag                                                                                                                                     <  1:131695/151‑1 (MQ=255)                                                                                                                                                                                                     cAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTAGAGAGTCAtgt                                      >  1:637006/1‑246 (MQ=255)                                                                                                                                                                                                       aaTAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTAGAGAGTCATGTGTGGCgg                               >  1:152474/1‑251 (MQ=255)                                                                                                                                                                                                       aaTAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTAGAGAGTCATGTGTGGCgg                               <  1:70779/251‑1 (MQ=255)                                                                                                                                                                                                         tAGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTAGAGAGTCATGTGTGGCGGAc                             <  1:69250/251‑1 (MQ=255)                                                                                                                                                                                                          aGGTAGCGCGAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTagag                                              <  1:315658/233‑1 (MQ=255)                                                                                                                                                                                                                  cgAATAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTAGAGAGTCATGTGTGGCGGACAGTACgac                     <  1:827/250‑3 (MQ=255)                                                                                                                                                                                                                     aTAGCCGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTtg                                                                          >  1:510161/1‑194 (MQ=255)                                                                                                                                                                                                                         ccGACCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTAGAGAGTCATGTGTGGCGGACAGTACGACGCAAGCt              <  1:40880/250‑1 (MQ=255)                                                                                                                                                                                                                            aCCCTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTAGAGAGTCATGTGTGGCGGACAGTACgac                     >  1:30338/1‑238 (MQ=255)                                                                                                                                                                                                                             cccTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTAGAGAGTCATGTGTGGCGGACAGTACGACGCAAGCTGCCGt         <  1:418086/251‑1 (MQ=255)                                                                                                                                                                                                                               cTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAAt                                                                                               >  1:47932/1‑163 (MQ=255)                                                                                                                                                                                                                               cTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTAGAGAGTCAtgtg                                     <  1:91030/221‑1 (MQ=255)                                                                                                                                                                                                                               cTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTAGAGAGTCATGTGTGGCg                                >  1:822816/1‑226 (MQ=255)                                                                                                                                                                                                                               cTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTAGAGAGTCATGTGTGGCGGACAGTACGACGCAAGCTGCCGTCg       >  1:707734/1‑251 (MQ=255)                                                                                                                                                                                                                               cTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTAGAGAGTCATGTGTGGCGGACAGTACGACGCAAGCTGCCGTCg       >  1:874344/1‑251 (MQ=255)                                                                                                                                                                                                                               cTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTAGAGAGTCATGTGTGGCGGACAGTACGACGCAAGCTGCCGTCg       >  1:515195/1‑251 (MQ=255)                                                                                                                                                                                                                               cTACTGAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTAGAGAGTCATGTGTGGCGGACAGTACGACGCAAGCTGCCGTCg       >  1:521968/1‑251 (MQ=255)                                                                                                                                                                                                                                    gAACTGCGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTAGAGAGTCATGTGTGGCGGACAGTACGACGCAAGCTGCCGTCGGGaaa  >  1:694440/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     CGGCAAGCAAAATTTCATTTTCCGCCGGCGGCATCAGTTGCTTGCGCAATGCGATCACATCGGGCAAATCCAGCTCATAGAACGGCACATTTTTGCCGATGTCACCCACGCGCTCATGACGCGCATCCAGCCCGCAACCGATCATCACGATAATCGGTTGTTGGTGCTGTTGAATAAAGGCTTTGGTGAGGCTATCCAAATAGGTAGCGCGAATAGCCGACCCTACTGAACTGTGGATAGCAGTGTCAAACTTACTGAAATCATAATCAATTTGCGGCACCAGGCGTAGCGCCGATGCGTCGTGAATAATCGGATCGGGTTTGAGGGACTCGTGATAGCGCATATAGAGCGGAATAAGCAGGGTCTCGCTGACCGCATCCTGAATTTAAACATCATTACCTCCTTGTGGAAACGCGCGGTTAAACCGCGTAGAGAGTCATGTGTGGCGGACAGTACGGCGCAAGCTGCCGTCGGGAAA  >  1/1567688‑1568165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑