Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,566,266	G→A	intergenic (‑330/‑29)	unknown ← / → unknown	oxidoreductase of aldo/keto reductase family, subgroup 1/Transcriptional regulator, LysR family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,566,266	0	G	A	100.0%	119.0 / NA	44	intergenic (‑330/‑29)	unknown/unknown	oxidoreductase of aldo/keto reductase family, subgroup 1/Transcriptional regulator, LysR family
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/21);  total (23/21)

CTCGGAGGGAGAGGGGAAAATCAATATCGTTCCCCCTCTCTCTGACGAAAATTGCCTGACGCAATTTCCGTCTCTCTCTCCCTCAAGCGGGCGAGAGGATTATTCCGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTGAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACATCAAACTGTTCAACCGCACCACACGCAGCATTGCCACCACGGAGGCGGGCGAGCAGTTGTTTCAAAATCTCTTGCCG  >  1/1566085‑1566510                                                                                                                                                                                      |                                                                                                                                                                                                                                                     ataaaacgacgtggaggtaacgcgtatCGTTCCCCCTCTCTCTGACGAAAATTGTCTGACGCAATTTCCGTCTCTCTCTCCCTCAAGCGGGCGAGAGGATTATTCCGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACcg                                                                                                                                                                                  >  1:841101/25‑250 (MQ=255)         acgtggaggtaacgcgtatCGTTCCCCCTCTCTCTGACGAAAATTGTCTGACGCAATTTCCGTCTCTCTCTCCCTCAAGCGGGCGAGAGGATTATTCCGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGAt                                                                                                                                                                                                                     <  1:281062/191‑1 (MQ=255)              gaggtaacgcgtatCGTTCCCCCTCTCTCTGACGAAAATTGTCTGACGCAATTTCCGTCTCTCTCTCCCTCAAGCGGGCGAGAGGATTATTCCGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAaat                                                                                                                                                                                                                                                   <  1:174746/161‑1 (MQ=255)              gaggtaacgcgtatCGTTCCCCCTCTCTCTGACGAAAATTGTCTGACGCAATTTCCGTCTCTCTCTCCCTCAAGCGGGCGAGAGGATTATTCCGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAg                                                                                                                                                                                                                                    <  1:780006/176‑1 (MQ=255)              gaggtaacgcgtatCGTTCCCCCTCTCTCTGACGAAAATTGTCTGACGCAATTTCCGTCTCTCTCTCCCTCAAGCGGGCGAGAGGATTATTCCGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAgg                                                                                                                                                                     >  1:609351/12‑250 (MQ=255)                  taacgcgtatCGTTCCCCCTCTCTCTGACGAAAATTGTCTGACGCAATTTCCGTCTCTCTCTCCCTCAAGCGGGCGAGAGGATTATTCCGCGTTTCGTTTTGATTTGTGCATTATAAAAATCTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGt                                                                                                                                                                 <  1:417501/243‑1 (MQ=255)                     cgcgtatCGTTCCCCCTCTCTCTGACGAAAATTGTCTGACGCAATTTCCGTCTCTCTCTCCCTCAAGCGGGCGAGAGGATTATTCCGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGAt                                                                                                                                                                                                                     <  1:250396/191‑1 (MQ=255)                     cgcgtatCGTTCCCCCTCTCTCTGACGAAAATTGTCTGACGCAATTTCCGTCTCTCTCTCCCTCAAGCGGGCGAGAGGATTATTCCGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCAc                                                                                                                                                                                    >  1:183131/5‑228 (MQ=255)                         tatCGTTCCCCCTCTCTCTGACGAAAATTGTCTGACGCAATTTCCGTCTCTCTCTCCCTCAAGCGGGCGAGAGGATTATTCCGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCaa                                                                                                                                                         >  1:744488/1‑251 (MQ=255)                         tatCGTTCCCCCTCTCTCTGACGAAAATTGTCTGACGCAATTTCCGTCTCTCTCTCCCTCAAGCGGGCGAGAGGATTATTCCGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCaa                                                                                                                                                         >  1:175768/1‑251 (MQ=255)                           tCGTTCCCCCTCTCTCTGACGAAAATTGTCTGACGCAATTTCCGTCTCTCTCTCCCTCAAGCGGGCGAGAGGATTATTCCGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAg                                                                                                                                                       >  1:536780/1‑251 (MQ=255)                           tCGTTCCCCCTCTCTCTGACGAAAATTGTCTGACGCAATTTCCGTCTCTCTCTCCCTCAAGCGGGCGAGAGGATTATTCCGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAg                                                                                                                                                       >  1:525282/1‑251 (MQ=255)                                                                aTTTCCGTCTCTCTCTCCCTCAAGCGGGCGAGAGGATTATTCCGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAg                                                                                                                  >  1:716934/1‑251 (MQ=255)                                                                aTTTCCGTCTCTCTCTCCCTCAAGCGGGCGAGAGGATTATTCCGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAg                                                                                                                  >  1:166508/1‑251 (MQ=255)                                                                                           gCGAGAGGATTATTCCGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGAAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAAc                                                                                        <  1:599642/250‑1 (MQ=255)                                                                                           gCGAGAGGATTATTCCGCGTTTCGTTTTGATTTGTGAATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAAc                                                                                        <  1:16529/250‑1 (MQ=255)                                                                                            cGAGAGGATTATTCCGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGcc                                                                                                                >  1:808707/1‑225 (MQ=255)                                                                                                 ggATTATTCCGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCaa                                                                                 >  1:829773/1‑251 (MQ=255)                                                                                                    ttattCCGCGTTTCGTTTTGTTTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGAATAGCCTTGCAAATAATATTCAACAATAGACCCCCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACAt                                                                              <  1:449476/251‑1 (MQ=255)                                                                                                    ttattCCGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACAt                                                                              <  1:64928/251‑1 (MQ=255)                                                                                                      attCCGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGac                                                                                                                                                                                                                 >  1:647723/1‑118 (MQ=255)                                                                                                      attCCGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGa                                                                                                                                                                                                      <  1:584309/129‑1 (MQ=255)                                                                                                         ccGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACCCCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACATCAAAc                                                                         <  1:138581/251‑1 (MQ=255)                                                                                                                 cgtttTGATTTGTGCATTAAAAAAACCTTTGATTCATTTGATTGGTAGAAAAAAAACGGATACCCTTGAAAAAAATATCCAACAAAAAAACCCCGCCTATGATCGGCAACAACAACGAACTTCGCACCTTTATCACCGCGGAGCAAGAAGGCAGTTTCCCCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACATCAAACTGTTCAAc                                                                 <  1:171899/251‑1 (MQ=255)                                                                                                                  gtttTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACATCAAACTGTTCAAc                                                                 >  1:587850/1‑250 (MQ=255)                                                                                                                    tttGATTTGTGCATTAAAAAAAATTTTGATTAATTTGATTAGTAGAAAAAAATCGAATAGCTTTGTAAAAAATATTCAACAATAGACCACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACATCAAACTGTTCAACCGc                                                              <  1:251992/251‑1 (MQ=255)                                                                                                                      tGATTTGTGCATTATAAAAAACTTTAATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATACAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACATCAAACTGTTCAACCGcac                                                            <  1:591242/251‑1 (MQ=255)                                                                                                                                 ttATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACATCAAACTGTTCAACCGCACCACACgcagca                                                 >  1:331281/1‑251 (MQ=255)                                                                                                                                   ataAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACATCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTCTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACATCCAACTGTTCAACCGCACCACACGCAGCAtt                                               >  1:592760/1‑251 (MQ=255)                                                                                                                                   ataAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACATCAAACTGTTCAACCGcac                                                            >  1:437147/1‑238 (MQ=255)                                                                                                                                   ataAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACATCAAACTGTTCAACCGCACCACACGCAGCAtt                                               >  1:720044/1‑251 (MQ=255)                                                                                                                                      aaaaaCTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACATCAAACTGTTCAACCGCACCACACGCAGCATTGcc                                            <  1:308798/251‑1 (MQ=255)                                                                                                                                         aaCTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACCCCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCTATTCGCAAGCTGGAAGAACAGCTCAACATCAAACTGTTCAACCGCACCACACGCAGCATTGctacc                                         <  1:138537/251‑1 (MQ=255)                                                                                                                                         aaCTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACATCAAACTGTTCAACCGCACCACACGCAGCATTGccacc                                         <  1:138549/251‑1 (MQ=255)                                                                                                                                                                 gAAAAAATCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACATCAAACTGTTCAACCGCACCACACGCAGCATTGCCACCACGGAGGCGGGCGAGCAGTTGttt                 >  1:215338/1‑251 (MQ=255)                                                                                                                                                                   aaaaaTCGGATAGCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACATCAAACTGTTCAACCGCACCACACGCAGCATTGCCACCACGGAGGCGGGCGAGCAGTTGTTTCa               <  1:897853/251‑1 (MQ=255)                                                                                                                                                                               gCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGAt                                                                                                                                                                                                                     <  1:514684/41‑1 (MQ=255)                                                                                                                                                                               gCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAAc                                                                                                                                      <  1:561380/120‑1 (MQ=255)                                                                                                                                                                               gCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACATCAAACTGTTCAACCGCACCACACGCAGCATTGCCACCACGGAGGCGGGCGAGCAGTTGTTTCAAAATCTCTTGc    >  1:594633/1‑250 (MQ=255)                                                                                                                                                                               gCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACATCAAACTGTTCAACCGCACCACACGCAGCATTGCCACCACGGAGGCGGGCGAGCAGTTGTTTCAAAATCTCTTGc    >  1:744328/1‑250 (MQ=255)                                                                                                                                                                               gCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACATCAAACTGTTCAACCGCACCACACGCAGCATTGCCACCACGGAGGCGGGCGAGCAGTTGTTTCAAAATCTCTTGc    >  1:142818/1‑250 (MQ=255)                                                                                                                                                                               gCCTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACATCAAACTGTTCAACCGCACCACACGCAGCATTGCCACCACGGAGGCGGGCGAGCAGTTGTTTCAAAATCTCTTGc    >  1:96730/1‑250 (MQ=255)                                                                                                                                                                                ccTTGTAAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACATCAAACTGTTCAACCGCACCACACGCAGCATTGCCACCACGGAGGCGGGCGAGCAGTTGTTTCAAAATCTCTTGCCg  >  1:141136/1‑251 (MQ=255)                                                                                                                                                                                ccTTGTAAATAATATTCAAAAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACATCAAACTGTTCAACCGCACCACACGCAGCATTGCCACCACGGAGGCGGGCGAGCAGTTGTTTCAAAATCTCTTGCCg  <  1:763640/251‑1 (MQ=255)                                                                                                                                                                                      |                                                                                                                                                                                                                                                     CTCGGAGGGAGAGGGGAAAATCAATATCGTTCCCCCTCTCTCTGACGAAAATTGCCTGACGCAATTTCCGTCTCTCTCTCCCTCAAGCGGGCGAGAGGATTATTCCGCGTTTCGTTTTGATTTGTGCATTATAAAAAACTTTGATTCATTTGATTAGTAGGAAAAAATCGGATAGCCTTGTGAATAATATTCAACAATAGAACACCGCCTATGATCGACAACATCAACGAACTTCGCACCTTTATCACCGCGGCGCAAGAAGGCAGTTTCACCAAAGCCGCCGCAAAATTAAACGTTTCCACCTCCGCATTAAGCCATTCCATTCGCAAGCTGGAAGAACAGCTCAACATCAAACTGTTCAACCGCACCACACGCAGCATTGCCACCACGGAGGCGGGCGAGCAGTTGTTTCAAAATCTCTTGCCG  >  1/1566085‑1566510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑