Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,548,266	G→A	G1188D (GGC→GAC)	unknown →	Inner membrane component of TAM transport system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,548,266	0	G	A	100.0%	205.2 / NA	72	G1188D (GGC→GAC)	unknown	Inner membrane component of TAM transport system
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/34);  total (38/34)

TCTTTCGGGCAGGATTTAATTATTCGTAAAGGGCTCATCAGTTTTGTCGGTCAACCGTCGCAACCGATGCTGAATATTGAAGCAATTCGTAATCCGGAAGCCATGGAAGACGCAGGCGTTACAGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGGCGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTGGTCAGCGGCACCATTACACCGCGCTTACAGGTAAAATACGGCGTTGGGTTATTTGACGGTTTAGCCGAAGTGACTCTGCGTTAC  >  1/1548022‑1548501                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                            tctTTCGGGCAGGATTTAATTATTCGTAAAGGGCTCATCAGTTTTGTCGGTCAACCGTCGCAGCCGATGCTGAATATTGAAGCAATTCGTAATCCGGAAGCCATGGAAGACGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATAc                                                                                                                                                                                                                                       <  1:235531/251‑1 (MQ=255)              aTTTAATTATTCGTAAAGGGCTCATCAGTTTTGTCGGTCAACCGTCGCAACCGATGCTGAATATTGAAGCAATTCGTAATCCGGAAGCCATGGAAGACGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCgg                                                                                                                                                                                                                           <  1:492491/250‑1 (MQ=255)                ttAATTATTCGTAAAGGGCTCATCAGTTTTGTCGGTCAACCGTCGCAACCGATGCTGAATATTGAAGCAATTCGTAATCCGGAAGCCATGGAAGACGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTc                                                                                                                                                                                                                        <  1:677507/251‑1 (MQ=255)                      attCGTAAAGGGCTCATCAGTTTTGTCGGTCAACCGTCGCAACCGATGCTGAATATTGAAGCAATTCGTAATCCGGAAGCCATGGAAGACGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTggg                                                                                                                                                                                                                  >  1:868274/1‑251 (MQ=255)                         cGTAAAGGGCTCATCAGTTTTGTCGGTCAACCGTCGCAACCGATGCTGAATATTGAAGCAATTCGTAATCCGGAAGCCATGGAAGACGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGgcg                                                                                                                                                                                                                >  1:608647/1‑250 (MQ=255)                               gggCTCATCAGTTTTGTCGGTCAACCGTCGCAACCGATGCTGAATATTGAAGCAATTCGTAATCCGGAAGCCATGGAAGACGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCttt                                                                                                                                                                                                         >  1:297078/1‑251 (MQ=255)                                 gCTCATCAGTTTTGTCGGTCAACCGTCGCAACCGATGCTGAATATTGAAGCAATTCGTAATCCGGAAGCCATGGAAGACGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTg                                                                                                                                                                                                        >  1:818191/1‑250 (MQ=255)                                         gTTTTGTCGGTCAACCGTCGCAACCGATGCTGAATATTGAAGCAATTCGTAATCCGGAAGCCATGGAAGACGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCg                                                                                                                                                                                                                                     >  1:379191/1‑213 (MQ=255)                                         gTTTTGTCGGTCAACCGTCGCAACCGATGCTGAATATTGAAGCAATTCGTAATCCGGAAGCCATGGAAGACGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATg                                                                                                                                                                                               >  1:13503/1‑251 (MQ=255)                                            ttGTCGGTCAACCGTCGCAACCGATGCTGAATATTGAAGCAATTCGTAATCCGGAAGCCATGGAAGACGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATgggg                                                                                                                                                                                            <  1:305536/251‑1 (MQ=255)                                             tGTCGGTCAACCGTCGCAACCGATGCTGAATATTGAAGCAATTCGTAATCCGGAAGCCATGGAAGACGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGc                                                                                                                                                                                           <  1:405626/251‑1 (MQ=255)                                                             cAACCGATGCTGAATATTGAAGCAATTCGTAATCCGGAAGCCATGGAAGACGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCtgtg                                                                                                                                                                                                                    >  1:103465/1‑210 (MQ=255)                                                              aaCCGATGCTGAATATTGAAGCAATTCGTAATCCGGAAGCCATGGAAGACGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAG‑AAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAAtg                                                                                                                                                                         <  1:117127/251‑1 (MQ=255)                                                               aCCGATGCTGAATATTGAAGCAATTCGTAATCCGGAAGCCATGGAAGACGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAAtg                                                                                                                                                                         <  1:241953/251‑1 (MQ=255)                                                                        gAATATTGAAGCAATTCGTAATCCGGAAGCCATGGAAGACGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAg                                                                                                                                                                                 >  1:272419/1‑234 (MQ=255)                                                                        gAATATTGAAGCAATTCGTAATCCGGAAGCCATGGAAGACGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCgggg                                                                                                                                                                >  1:77677/1‑251 (MQ=255)                                                                                   cAATTCGTAATCCGGAAGCCATGGAAGACGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGaaaa                                                                                                                                                     <  1:126043/251‑1 (MQ=255)                                                                                      ttCGTAATCCGGAAGCCATGGAAGACGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGc                                                                                                                                                   >  1:497042/1‑250 (MQ=255)                                                                                             tCCGGAAGCCATGGAAGACGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTa                                                                                                                                           >  1:253074/1‑251 (MQ=255)                                                                                             tCCGGAAGCCATGGAAGACGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTa                                                                                                                                           <  1:31120/251‑1 (MQ=255)                                                                                                            aGACGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAAc                                                                                                                             >  1:330104/1‑250 (MQ=255)                                                                                                              aCGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAAcc                                                                                                                            <  1:557757/249‑1 (MQ=255)                                                                                                              aCGCAGGCGTTACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAAc                                                                                                                             >  1:837418/1‑248 (MQ=255)                                                                                                                       ttACTGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAAc                                                                                                                             <  1:40166/239‑1 (MQ=255)                                                                                                                           tgCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCgggg                                                                                                             <  1:467993/250‑1 (MQ=255)                                                                                                                           tgCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCgggg                                                                                                             <  1:644632/250‑1 (MQ=255)                                                                                                                                gtgtGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTggg                                                                                                         >  1:206660/1‑250 (MQ=255)                                                                                                                                gtgtGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCACAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTggg                                                                                                         >  1:461864/1‑250 (MQ=255)                                                                                                                                  gtgAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGa                                                                                                      >  1:222703/1‑251 (MQ=255)                                                                                                                                            aTGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAAc                                                                                                                             <  1:383033/218‑1 (MQ=255)                                                                                                                                            aTGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTaaa                                                                                            >  1:223013/1‑251 (MQ=255)                                                                                                                                            aTGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTaaa                                                                                            >  1:81946/1‑251 (MQ=255)                                                                                                                                            aTGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTaaa                                                                                            >  1:452445/1‑251 (MQ=255)                                                                                                                                             tGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAag                                                                                           >  1:798283/1‑251 (MQ=255)                                                                                                                                              ggTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTc                                                                                                                                                                    <  1:362487/177‑1 (MQ=255)                                                                                                                                              ggTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAagt                                                                                          >  1:884540/1‑251 (MQ=255)                                                                                                                                              ggTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAag                                                                                           <  1:367336/250‑1 (MQ=255)                                                                                                                                               gTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAg                                                                                                                                                                                 >  1:597606/1‑163 (MQ=255)                                                                                                                                               gTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGATTCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAagta                                                                                         >  1:894009/1‑251 (MQ=255)                                                                                                                                               gTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAAcc                                                                                                                            >  1:609629/1‑216 (MQ=255)                                                                                                                                               gTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCgggg                                                                                                             <  1:386381/231‑1 (MQ=255)                                                                                                                                               gTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAagta                                                                                         >  1:372141/1‑251 (MQ=255)                                                                                                                                                tataGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAagtag                                                                                        <  1:339803/251‑1 (MQ=255)                                                                                                                                                     cGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTgg                                                                                     <  1:203288/249‑1 (MQ=255)                                                                                                                                                                aGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTGGTCAGCGGCACCAt                                                                        >  1:723042/1‑251 (MQ=255)                                                                                                                                                                 gtgtAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATgggg                                                                                                                                                                                            <  1:461732/134‑1 (MQ=255)                                                                                                                                                                   gtAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAg                                                                                                                                     <  1:517768/187‑1 (MQ=255)                                                                                                                                                                    tAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTGGTCAGCGGCACCATTac                                                                     <  1:143414/250‑1 (MQ=255)                                                                                                                                                                     aaCCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTGGTCAGCGGCACCATTacac                                                                   <  1:195261/251‑1 (MQ=255)                                                                                                                                                                       ccATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTGGTCAGCGGCACCATTACACcg                                                                 >  1:600865/1‑251 (MQ=255)                                                                                                                                                                        cATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAagt                                                                                          >  1:466457/1‑225 (MQ=255)                                                                                                                                                                                         gAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTGGTCAGCGGCACCATTACACCGCGCTTACAGGTAAAATa                                                <  1:459341/250‑1 (MQ=255)                                                                                                                                                                                              ccGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTGGTCAGCGGCACCATTACACCGCGCTTACAGGTAAAATACGGCGt                                          >  1:368626/1‑251 (MQ=255)                                                                                                                                                                                                   ggATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTGGTCAGCGGCACCATTacac                                                                   <  1:234796/221‑1 (MQ=255)                                                                                                                                                                                                   ggATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTGGTCAGCGGCACCATTACACCGCGCTTACAGGTAAAATACGGCGTTGGGt                                     >  1:117808/1‑251 (MQ=255)                                                                                                                                                                                                     aTCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCt                                                                                                                                                                                      >  1:706996/1‑104 (MQ=255)                                                                                                                                                                                                     aTCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTAccgc                                                                                                                  <  1:569850/172‑1 (MQ=255)                                                                                                                                                                                                     aTCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTGGTCAGCGGCACCATTACACCGCGCTTACAg                                                        <  1:885871/230‑1 (MQ=255)                                                                                                                                                                                                     aTCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTGGTCAGCGGCACCATTACACCGCGCTTACAGGTAAAATACGGCGTTGGGTTa                                   >  1:468933/1‑251 (MQ=255)                                                                                                                                                                                                          gCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTGGTCAGCGGCACCATTACACCGCGCTTACAGGTAAAATACGGCGTTGGGTTAttt                                <  1:257654/249‑1 (MQ=255)                                                                                                                                                                                                          gCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTGGTCAGCGGCACCATTACACCGCGCTTACAGGTAAAATACGGCGTTGGGTTATTTGa                              >  1:323667/1‑251 (MQ=255)                                                                                                                                                                                                               aTCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTgg                                                                                     <  1:116215/191‑1 (MQ=255)                                                                                                                                                                                                               aTCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTGGTCAGCGGCACCATTACACCGCGCTTACAGGTAAAATACGGCGTTGGGTTATTTGACGGtt                         >  1:249455/1‑251 (MQ=255)                                                                                                                                                                                                               aTCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTGGTCAGCGGCACCATTACACCGCGCTTACAGGTAAAATACGGCGTTGGGTTATTTGACGGtt                         >  1:245809/1‑251 (MQ=255)                                                                                                                                                                                                                 cTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTg                                                                                                                                              <  1:287701/132‑1 (MQ=255)                                                                                                                                                                                                                    atatTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTGGTCAGCGGCACCATTacac                                                                   >  1:611508/1‑204 (MQ=255)                                                                                                                                                                                                                     tatTTTAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTGGTCAGCGGCACCATTACACCGCGCTTACAGGTAAAATACGGCGTTGGGTTATTTGACGGTTTAGCCg                   <  1:864217/251‑1 (MQ=255)                                                                                                                                                                                                                          tAACCGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAag                                                                                           >  1:273812/1‑174 (MQ=255)                                                                                                                                                                                                                              cGGCCGCTCCTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTGGTCAGCGGCACCATTACACCGCGCTTACAGGTAAAATACGGCGTTGGGTTATTTGACGGTTTAGCCGAAGTGActc          <  1:887244/251‑1 (MQ=255)                                                                                                                                                                                                                                      ccTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCAt                                                                                                                                                                                                <  1:250963/61‑1 (MQ=255)                                                                                                                                                                                                                                      ccTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTGGTCAGCGGCACCATTACACCGCGCTTACAGGTAAAATACGGCGTTGGGTTATTTGACGGTTTAGCCGAAGTGACTCTGCGTTa   >  1:865428/1‑250 (MQ=255)                                                                                                                                                                                                                                      ccTTAGAAAACAGCGACGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTGGTCAGCGGCACCATTACACCGCGCTTACAGGTAAAATACGGCGTTGGGTTATTTGACGGTTTAGCCGAAGTGACTCTGCGTTAc  <  1:766691/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                            TCTTTCGGGCAGGATTTAATTATTCGTAAAGGGCTCATCAGTTTTGTCGGTCAACCGTCGCAACCGATGCTGAATATTGAAGCAATTCGTAATCCGGAAGCCATGGAAGACGCAGGCGTTACAGCCGGTGTGAAAGTGAATGGTATAGCGACCGCACCCAGTGTAACCATTTTCTCCGAACCGGGAATGCCGCAGGATCAGGCACTATCTTATATTTTAACCGGCCGCTCCTTAGAAAACAGCGGCGATACCGCTTCCAGCGGTTCTGTGGGCGCCGCTTTGCTCGGCATGGGGCTCGCTAAAAGCGGTAATGTGGTCGGGGGAATCGGAAAAGCCTTTGGTATCAAAGATCTGAACCTGAGTACCGCCGGGGTGGGCGACAGATCTAAAGTAGTGGTCAGCGGCACCATTACACCGCGCTTACAGGTAAAATACGGCGTTGGGTTATTTGACGGTTTAGCCGAAGTGACTCTGCGTTAC  >  1/1548022‑1548501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑