Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,546,381	C→T	H560Y (CAT→TAT)	unknown →	Inner membrane component of TAM transport system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,546,381	0	C	T	100.0%	204.8 / NA	74	H560Y (CAT→TAT)	unknown	Inner membrane component of TAM transport system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/48);  total (26/48)

TTGGCAAAAATCAATGTGGGCAAATACTTATCCGTCTTCCCGGCCCAGTTATCAGGCAAATTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCCATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAAC‑ACATGCGCCTGAACCAAGCCCTGATTAAAGGCGAAATCAACAGCGCGGAACAAATTAGCGGTCAG  >  1/1546135‑1546611                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          ttGGCAAAAATCAATGTGGGCAAATACTTATCCGTCTTCCCGGCCCAGTTATCAGGCAAATTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTAtct                                                                                                                                                                                                                                       <  1:714580/251‑1 (MQ=255) ttGGCAAAAATCAATGTGGGCAAATACTTATCCGTCTTCCCGGCCCAGTTATCAGGCAAATTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTAtct                                                                                                                                                                                                                                       <  1:144341/251‑1 (MQ=255) ttGGCAAAAATCAATGTGGGCAAATACTTATCCGTCTTCCCGGCCCAGTTATCAGGCAAATTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTAtct                                                                                                                                                                                                                                       <  1:216774/251‑1 (MQ=255) ttGGCAAAAATCAATGTGGGCAAATACTTATCCGTCTTCCCGGCCCAGTTATCAGGCAAATTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTAtct                                                                                                                                                                                                                                       <  1:653666/251‑1 (MQ=255)  tGGCAAAAATCAATGTGGGCAAATACTTATCCGTCTTCCCGGCCCAGTTATCAGGCAAATTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTAtctc                                                                                                                                                                                                                                      <  1:792803/251‑1 (MQ=255)      aaaaaTCAATGTGGGCAAATACTTATCCGTCTTCCCGGCCCAGTTATCAGGCAAATTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTg                                                                                                                                                                                                                                  <  1:356025/251‑1 (MQ=255)        aaaTCAATGTGGGCAAATACTTATCCGTCTTCCCGGCCCAGTTATCAGGCAAATTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGAt                                                                                                                                                                                                                                <  1:25751/251‑1 (MQ=255)         aaTCAATGTGGGCAAATACTTATCCGTCTTCCCGGCCCAGTTATCAGGCAAATTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATa                                                                                                                                                                                                                               >  1:406478/1‑251 (MQ=255)                   ggCAAATACTTATCCGTCTTCCCGGCCCAGTTATCAGGCAAATTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATAc                                                                                                                                                                                                                              >  1:885381/1‑242 (MQ=255)                   ggCAAATACTTATCCGTCTTCCCGGCCCAGTTATCAGGCAAATTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGAttt                                                                                                                                                                                                                     >  1:875234/1‑251 (MQ=255)                        aTACTTATCCGTCTTCCCGGCCCAGTTATCAGGCAAATTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAAc                                                                                                                                                                                                                 >  1:13377/1‑250 (MQ=255)                           cTTATCCGTCTTCCCGGCCCAGTTATCAGGCAAATTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAAc                                                                                                                                                                                                                 >  1:679100/1‑247 (MQ=255)                           cTTATCCGTCTTCCCGGCCCAGTTATCAGGCAAATTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTgg                                                                                                                                                                                                             >  1:553818/1‑251 (MQ=255)                               tCCGTCTTCCCGGCCCAGTTATCAGGCAAATTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACAt                                                                                                                                                                                                         >  1:755324/1‑251 (MQ=255)                                   tCTTCCCGGCCCAGTTATCAGGCAAATTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTaa                                                                                                                                                                                                      <  1:225661/250‑1 (MQ=255)                                   tCTTCCCGGCCCAGTTATCAGGCAAATTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTaa                                                                                                                                                                                                      <  1:458539/250‑1 (MQ=255)                                                gTTATCAGGCAAATTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACa                                                                                                                                                                                                          >  1:36860/1‑233 (MQ=255)                                                  tATCAGGCAAATTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAg                                                                                                                                                                                      <  1:136347/251‑1 (MQ=255)                                                       ggCAAATTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATgc                                                                                                                                                                                                   >  1:566209/1‑233 (MQ=255)                                                          aaaTTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTtgtg                                                                                                                                                                              <  1:284632/251‑1 (MQ=255)                                                           aaTTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATAc                                                                                                                                                                                                                              <  1:779697/202‑1 (MQ=255)                                                                 gCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTaa                                                                                                                                                                                                      <  1:610095/220‑1 (MQ=255)                                                                 gCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATg                                                                                                                                                                                                    >  1:358528/1‑222 (MQ=255)                                                                 gCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGAt                                                                                                                                                                       >  1:41509/1‑251 (MQ=255)                                                                   tCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAggg                                                                                                                                                                                    <  1:276892/236‑1 (MQ=255)                                                                         ggCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATcgg                                                                                                                                                                >  1:784830/1‑250 (MQ=255)                                                                          gCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATcggc                                                                                                                                                               <  1:181335/250‑1 (MQ=255)                                                                          gCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATcgg                                                                                                                                                                <  1:641284/249‑1 (MQ=255)                                                                                      aaaGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACCGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGc                                                                                                                                                 <  1:204577/251‑1 (MQ=255)                                                                                             ttGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTa                                                                                                                                           <  1:547968/250‑1 (MQ=255)                                                                                                 cAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATGGGATTTTAACCTGGACATTAATGCGCGGAATTTACAGGGCTTGTGGGCGGAGTTATCGGC‑GGGTTTGAAAGGCAACGTAAAAt                                                                                                                                       <  1:838119/250‑1 (MQ=255)                                                                                                                gAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGGTTGTAAGGGAAAGGAAAATTAAAAGGCAGCATCg                                                                                                                        >  1:659334/1‑250 (MQ=255)                                                                                                                         atTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACa                                                                                                                                                                                       >  1:633795/1‑179 (MQ=255)                                                                                                                         atTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGgc                                                                                                                               <  1:25732/234‑1 (MQ=255)                                                                                                                          ttCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTtg                                                                                                                                                                                >  1:818111/1‑185 (MQ=255)                                                                                                                            cAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAgg                                                                                                                                                  <  1:774399/212‑1 (MQ=255)                                                                                                                                  acacTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGCGGGGTTTAAAAGG‑AACGTAAAAATAAAAGGCAGCAACGCCCCACCaaccaatcctt                                                                                                      >  1:514220/1‑241 (MQ=255)                                                                                                                                    acTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑gg                                                                                                  <  1:478852/251‑1 (MQ=255)                                                                                                                                     cTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGcc                                                                                                                      <  1:692436/231‑1 (MQ=255)                                                                                                                                       gTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAAt                                                                                                                                       <  1:606204/212‑1 (MQ=255)                                                                                                                                        tCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGAtt                                                                                               <  1:477503/250‑1 (MQ=255)                                                                                                                                         cccAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGCGGGGTTTGAAAGGAAAGGAAAAATAAAAC‑GCAGCATCcccccccccaacaatcatgcggggttt                                                                                              >  1:703106/1‑228 (MQ=255)                                                                                                                                                  cGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTTAAAGGCAACGTAAAAATAAAAAGCCGCAACGCCCCCCCCAACATTCATTC‑GGAATTAAACGCaaa                                                                                     >  1:147894/1‑249 (MQ=255)                                                                                                                                                                    aTTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAac‑ac                                                                 <  1:90617/251‑1 (MQ=255)                                                                                                                                                                       tCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAAC‑ACATg                                                              <  1:834135/251‑1 (MQ=255)                                                                                                                                                                          cTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAAC‑ACATgcgc                                                           <  1:570142/251‑1 (MQ=255)                                                                                                                                                                          cTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCACACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAAC‑ACATgcgc                                                           <  1:283183/251‑1 (MQ=255)                                                                                                                                                                                 cAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAAC‑ACATGCGCCTGAAc                                                     <  1:872960/250‑1 (MQ=255)                                                                                                                                                                                 cAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAAC‑ACATGCGCCTGAAc                                                     <  1:239527/250‑1 (MQ=255)                                                                                                                                                                                  aaTCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAac‑ac                                                                 <  1:272088/237‑1 (MQ=255)                                                                                                                                                                                   aTCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTaa                                                                                                                                                                                                      <  1:851519/106‑1 (MQ=255)                                                                                                                                                                                   aTCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTTTAAAGCAACGTAAAAATAAAAGGCAGCAACGCCCCACCCAGCATTTATGCGGGGTTTAA‑CGCAAACCGCCTTGCATTTCAAC‑ACAAGAGCCCGAAcccg                                                  >  1:360750/1‑249 (MQ=255)                                                                                                                                                                                     cagcagTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAAc                                                                                           <  1:722612/209‑1 (MQ=255)                                                                                                                                                                                       gcagTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGTAAAGCAACGTAAAAATAAAAAGCAGCAACGCCCGACCCAGCAATTATTCGGGATTTAACC‑CAAACCCCCTTGCATTCCcac‑acaaacgccccaaccaaacc                                               >  1:75555/1‑233 (MQ=255)                                                                                                                                                                                        cagTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAAC‑ACATGCGCCTGAACCAAGccc                                              <  1:475255/250‑1 (MQ=255)                                                                                                                                                                                        cagTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAAC‑ACATGCGCCTGAACCAAGcc                                               <  1:734972/249‑1 (MQ=255)                                                                                                                                                                                            tGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAAC‑ACATGCGCCTGAACCAAGCCCTGAtt                                         <  1:490719/251‑1 (MQ=255)                                                                                                                                                                                                  atatCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGGAAGGGAACGGAAAAATAAAAGGCAGCATCGCCCCCCCAACCATTCATTC‑GGGTTTTACCGCAAACCGCCTTTCATTCCcac‑acacacgcc                                                          >  1:463548/1‑222 (MQ=255)                                                                                                                                                                                                  atatCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAAC‑ACATGCGCCTGAACCAAGCCCTGATTAAAGGc                                   <  1:566063/251‑1 (MQ=255)                                                                                                                                                                                                          cAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAAC‑ACATGCGcc                                                          <  1:5316/220‑1 (MQ=255)                                                                                                                                                                                                            gTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTAtct                                                                                                                                                                                                                                       >  1:278632/1‑48 (MQ=255)                                                                                                                                                                                                            gTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGGTTTAAAGGCAACGTAAAAATAAACAGCAGCATCCCCCCACCAAACATTCATTC‑GGGTTTAAACCCAACCCGCCTTTCCTTCCAAC‑ACAACCGCCCCAACCAAACCCCTATTAAAAGCGAAAacaaca                         >  1:527246/1‑251 (MQ=255)                                                                                                                                                                                                             ttATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAAC‑ACATGCGCCTGAACCAAGCCCTGATTAAAGGCGAAATCAACAg                        <  1:214470/251‑1 (MQ=255)                                                                                                                                                                                                                ttGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAac‑ac                                                                 <  1:800147/207‑1 (MQ=255)                                                                                                                                                                                                                     cACTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGA‑GGGTTTGAAAGGCAACGTAAAATTAAAAGGCAGCATCGCCCGaaaa                                                                                                                <  1:3310/157‑4 (MQ=255)                                                                                                                                                                                                                       cTTATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAac‑ac                                                                 <  1:387541/200‑1 (MQ=255)                                                                                                                                                                                                                         tATGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTATAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAAC‑ACATGCGCCTGAACCAAGCCCTGATTAAAGGCGAAATCAACAGCGCGGAACAAAt            <  1:453277/251‑1 (MQ=255)                                                                                                                                                                                                                          aTGGCGACAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAAC‑ACATGCGCCTGAACCAAGCCCTGATTAAAGGCGAAATCAACAGCGCGGAACAAAtt           <  1:349206/251‑1 (MQ=255)                                                                                                                                                                                                                                aCAATAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAAC‑ACATGCGCCTGAACCAAGCCCTGATTAAAGGCGAAATCAACAGCGCGGAACAAATTAGCgg      <  1:719162/250‑1 (MQ=255)                                                                                                                                                                                                                                   aTAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATcgg                                                                                                                                                                >  1:25788/1‑96 (MQ=255)                                                                                                                                                                                                                                   aTAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAAGGAACGTAAAAATAAAAAGCAGCATCGCCCCCCCAAGCATTCATGC‑GGGTTTTACCCCCACCCGCTTTTCCTTCCCACAACA‑GCGCCCGAACCAAACCCCCGTTAAAAGCGAAAACAACAACGCCGAAccaaataacggcggg  >  1:491240/1‑237 (MQ=255)                                                                                                                                                                                                                                   aTAAAATTGAGGTGCAGGGCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGGTTGAAAAGCAACGTAAAATTAAAAAGCAGCATCGCCCCACCAAGCAATCATGC‑GGATTTAACCGCAAACCGCTTTGCATTCCCAC‑ACATGCGCCTGAACCAAACCCCGATTAAAgg                                    >  1:337532/1‑217 (MQ=255)                                                                                                                                                                                                                                                gCAGGGCTATCTCGGTGATACATCGGATTTTAGCCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAAC‑ACATGCGCCTGAACCAAGCCCTGATTAAAGGCGAAATCAACAGCGCGGAAc                <  1:385964/224‑1 (MQ=255)                                                                                                                                                                                                                                                   gggCTATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAac‑ac                                                                 <  1:750139/172‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          TTGGCAAAAATCAATGTGGGCAAATACTTATCCGTCTTCCCGGCCCAGTTATCAGGCAAATTAAGCTCACAAGGCAAAGCCAATCAAAGCGGTTGGCAGGTTGCCGTGCCGGAATTGGATATTCAAGGCACACTGTCCCAACGCCCGCTGAACCTGAAAGGCAATTTCACTGCGGGCAATCAGCAGTTGCTCAATATCCCGCAGTTATTGCTCACTTATGGCGACAATAAAATTGAGGTGCAGGGCCATCTCGGTGATACATCGGATTTTAACCTGGACATTAATGCGCCGAATTTACAGGGCTTGTGGGCGGATTTATCGGC‑GGGTTTGAAAGGCAACGTAAAATTAAACGGCAGCATCGCCCGACCAAGCATTCATGC‑GGATTTAACCGCAAGCCGCTTTGCATTCCAAC‑ACATGCGCCTGAACCAAGCCCTGATTAAAGGCGAAATCAACAGCGCGGAACAAATTAGCGGTCAG  >  1/1546135‑1546611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑