Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	299,798	C→T	P565P (CCG→CCA)	unknown ←	Cytochrome c‑type biogenesis protein DsbD, protein‑disulfide reductase (EC 1.8.1.8)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	299,798	0	C	T	100.0%	112.6 / NA	41	P565P (CCG→CCA)	unknown	Cytochrome c‑type biogenesis protein DsbD, protein‑disulfide reductase (EC 1.8.1.8)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/17);  total (24/17)

GCATAACCTGAAGTAATGCGTAATAAGCCTAAAAAGTAATTGCTGTATTTGTTTAAAAACATTTTTTCTTCCTTAAATAAAATAATGAAAATTGAAGGCGCGTATGCTAACAGTAAATTTAAAAGAAAAAAACAATATTGGTTAATTATTTAACGCTTTTGCTGCTTGTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGCGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTATGTTCAAATTCTTTACAAGCGACGCACCAATCCGCGTAAAGATCCAATAGCGCCAGTGGTTTCGGGTTCTTCGTTAATACCAGTTGTAACTC  >  1/299561‑300037                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                gCATAACCTGAAGTAATGCGTAATAAGCCTAAAAAGTAATTGCTGTATTTGTTTAAAAACATTTTTTCTTCCTTAAATAAAATAATGAAAATTGAAGGCGCGTATGCTAACAGTAAATTTAAAAGAAAAAAACAATATTGGTTAATTATTTAACGCTTTTGCTGCTTGTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCa                                                                                                                                                                                                                                    >  1:286999/1‑251 (MQ=255)    tAACCTGAAGTAATGCGTAATAAGCCTAAAAAGTAATTGCTGTATTTGTTTAAAAACATTTTTTCTTCCTTAAATAAAATAATGAAAATTGAAGGCGCGTATGCTAACAGTAAATTTAAAAGAAAAAAACAATATTGGTTAATTATTTAACGCTTTTGCTGCTTGTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCa                                                                                                                                                                                                                                 >  1:35404/1‑251 (MQ=255)                              tAAAAAGTAATTGCTGTATTTGTTTAAAAACATTTTTTCTTCCTTAAATAAAATAATGAAAATTGAAGGCGCGTATGCTAACAGTAAATTTAAAAGAAAAAAACAATATTGGTTAATTATTTAACGCTTTTGCTGCTTGTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAAt                                                                                                                                                                                                       <  1:348030/251‑1 (MQ=255)                                           cTGTATTTGTTTAAAAACATTTTTTCTTCCTTAAATAAAATAATGAAAATTGAAGGCGCGTATGCTAACAGTAAATTTAAAAGAAAAAAACAATATTGGTTAATTATTTAACGCTTTTGCTGCTTGTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAg                                                                                                                                                                                          >  1:807352/1‑251 (MQ=255)                                           cTGTATTTGTTTAAAAACATTTTTTCTTCCTTAAATAAAATAATGAAAATTGAAGGCGCGTATGCTAACAGTAAATTTAAAAGAAAAAAACAATAGTGGTTAATTATTTAACGCTTATGCTGCTTGTACTGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTTCCTGTGGCTTAAAAACCATCAACGTCGGCAGGCCGGTTATTGACAATTGCTTCATTaacg                                                                                                                                                                                          >  1:839759/1‑249 (MQ=255)                                                        aaaaCATTTTTTCTTCCTTAAATAAAATAATGAAAATTGAAGGCGCGTATGCTAACAGTAAATTTAAAAGAAAAAAACAATATTGGTTAATTATTTAACGCTTTTGCTGCTTGTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTc                                                                                                                                                                             <  1:855648/251‑1 (MQ=255)                                                                                           aTTGAAGGCGCGTATGCTAACAGTAAATTTAAAAGAAAAAAACAATATTGGTTAATTATTTAACGCTTTTGCTGCTTGTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGAc                                                                                                                                                                                                          <  1:171015/187‑1 (MQ=255)                                                                                            ttGAAGGCGCGTATGCTAACAGTAAATTTAAAAGAAAAAAACAATATTGGTTAATTATTTAACGCTTTTGCTGCTTGTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCAt                                                                                                                                                              >  1:778247/1‑230 (MQ=255)                                                                                                   cgcgTATGCTAACAGTAAATTTAAAAGAAAAAAACAATATTGGTTAATTATTTAACGCTTTTGCTGCTTGTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCtttt                                                                                                                                  >  1:107793/1‑251 (MQ=255)                                                                                                      gTATGCTAACAGTAAATTTAAAAGAAAAAAACAATATTGGTTAATTATTTAACGCTTTTGCTGCTTGTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTaaaa                                                                                                                                                                                                                                        <  1:372826/146‑1 (MQ=255)                                                                                                         tGCTAACAGTAAATTTAAAAGAAAAAAACAATATTGGTTAATTATTTAACGCTTTTGCTGCTTGTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCg                                                                                                                            >  1:869262/1‑251 (MQ=255)                                                                                                                  tAAATTTAAAAGAAAAAAACAATATTGGTTAATTATTTAACGCTTTTGCTGCTTGTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCAc                                                                                                                    <  1:5935/250‑1 (MQ=255)                                                                                                                                  aaaCAATATTGGTTAATTATTTAACGCTTTTGCTGCTTGTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGttgtttgt                                                                                                                                                                 <  1:711684/189‑1 (MQ=255)                                                                                                                                            ggTTAATTATTTAACGCTTTTGCTGCTTGTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTATGtt                                                                                          >  1:598120/1‑250 (MQ=255)                                                                                                                                                          cGCTTTTGCTGCTTGTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTATg                                                                                            >  1:804219/1‑234 (MQ=255)                                                                                                                                                            cTTTTGCTGCTTGTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAg                                                                                                                              >  1:28692/1‑198 (MQ=255)                                                                                                                                                                    gctTGTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGGTTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTATGTTCAAATTCTTTACAAg                                                                           <  1:369438/241‑1 (MQ=255)                                                                                                                                                                    gctTGTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTATGTTCAAATTCTTTACAAGCGACGCACCa                                                                 >  1:217134/1‑251 (MQ=255)                                                                                                                                                                    gctTGTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTATGTTCAAATTCTTTACAAGCGACGCACCa                                                                 >  1:131509/1‑251 (MQ=255)                                                                                                                                                                     ctTGTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAg                                                                                                                                                                                          <  1:241961/129‑1 (MQ=255)                                                                                                                                                                        gTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCAc                                                                                                                    <  1:55645/196‑1 (MQ=255)                                                                                                                                                                             gTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCAc                                                                                                                    >  1:817344/1‑191 (MQ=255)                                                                                                                                                                                 gTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTtg                                                                                                                 >  1:599955/1‑190 (MQ=255)                                                                                                                                                                                   aaaaaTGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTATGTTCAAATTCTTTACAAGCGACGCACCAATCCGCGTAAAGATc                                                  <  1:543608/251‑1 (MQ=255)                                                                                                                                                                                        tGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAg                                                                                                                              <  1:817041/170‑1 (MQ=255)                                                                                                                                                                                          cTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTATGTTCAAATTCTTTACAAGCGACGCACCAATCCGCGTAAAGATCCAATAg                                            <  1:107849/250‑1 (MQ=255)                                                                                                                                                                                              tcCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTATGTTCAAATTCTTTACAAGCGACGCACCAATCCGCGTAAAGATCCAATAGCGCCa                                       >  1:137367/1‑251 (MQ=255)                                                                                                                                                                                              tcCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTATGTTCAAATTCTTTACAAGCGACGCACCAATCCGCGTAAAGATCCAATAGCGCCa                                       >  1:627139/1‑251 (MQ=255)                                                                                                                                                                                                   ccATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAAc                                                                                                                                                 <  1:362902/140‑1 (MQ=255)                                                                                                                                                                                                   ccATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGATTTATGTTCAAATTCTTTACAAGCGACGCACCAATc                                                              >  1:282163/1‑223 (MQ=255)                                                                                                                                                                                                           ccGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTa                                                                                              >  1:558213/1‑183 (MQ=255)                                                                                                                                                                                                           ccGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTATGTTCAAATTCTTTACAAGCGACGCAc                                                                   <  1:788212/210‑1 (MQ=255)                                                                                                                                                                                                              gTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTATGTTCAAATTCTTTACAAGCGACGCACCAATCCGCGTAAAGATCCAATAGCGCCAGTGGTTTCGGGttctt                       >  1:535296/1‑251 (MQ=255)                                                                                                                                                                                                              gTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTATGTTCAAATTCTTTACAAGCGACGCACCAATCCGCGTAAAGATCCAATAGCGCCAGTGGTTTCGGGttctt                       >  1:627995/1‑251 (MQ=255)                                                                                                                                                                                                              gTAATGCGGTGCGATGAAATTTCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTATGTTCAAATTCTTTACAAGCGACGCACCAATCCGCGTAAAGATCCAATAGCGCCAGTGGTTTCGGGttctt                       >  1:326705/1‑251 (MQ=255)                                                                                                                                                                                                                                 tttCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTATGTTCAAATTCTTTACAAGCGACGCACCAATCCGCGTAAAGATCCAATAGCGCCAGTGGTTTCGGGTTCTTCGTTAATACCAGTTGTaa     >  1:554849/1‑250 (MQ=255)                                                                                                                                                                                                                                   tCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTATg                                                                                            >  1:329913/1‑161 (MQ=255)                                                                                                                                                                                                                                   tCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTATGTTCAAATTCTTTACAAGCGACGCACCAATCCGCGTAAAGATCCAAt                                              >  1:481745/1‑207 (MQ=255)                                                                                                                                                                                                                                   tCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTATGTTCAAATTCTTTACAAGCGACGCACCAATCCGCGTAAAGATCCAATAGCGCCAGTGGTTTCGGGTTCTTCGTTAATACCAGTTGTAActc  <  1:503090/251‑1 (MQ=255)                                                                                                                                                                                                                                   tCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTATGTTCAAATTCTTTACAAGCGACGCACCAATCCGCGTAAAGATCCAATAGCGCCAGTGGTTTCGGGTTCTTCGTTAATACCAGTTGTAActc  <  1:554041/251‑1 (MQ=255)                                                                                                                                                                                                                                   tCTTTTCCCTGTGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTATGTTCAAATTCTTTACAAGCGACGCACCAATCCGCGTAAAGATCCAATAGCGCCAGTGGTTTCGGGTTCTTCGTTAATACCAGTTGTAActc  <  1:585963/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                GCATAACCTGAAGTAATGCGTAATAAGCCTAAAAAGTAATTGCTGTATTTGTTTAAAAACATTTTTTCTTCCTTAAATAAAATAATGAAAATTGAAGGCGCGTATGCTAACAGTAAATTTAAAAGAAAAAAACAATATTGGTTAATTATTTAACGCTTTTGCTGCTTGTAATGTTTGTAAAAATGCTTCTCCTTCCATAAAACCGGTAATGCGGTGCGATGAAATTTCTTTTCCCTGCGGGTTAAAAAGCATCAACGTCGGCAAGCCGGTTATTGACAATTGCTTCATTAAAGTGCGGTTGTTTTCGCTGTTGTTTGTCATGTCGATTCTTAACAGCAAAACGTCTTTTAAAGCGTCTTGCACTTGTGGTTCCGCAAAGGTTTTATGTTCAAATTCTTTACAAGCGACGCACCAATCCGCGTAAAGATCCAATAGCGCCAGTGGTTTCGGGTTCTTCGTTAATACCAGTTGTAACTC  >  1/299561‑300037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑