Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,537,093	A→G	L216L (TTG→CTG)	unknown ←	Uncharacterized protein EC‑HemX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,537,093	0	A	G	100.0%	196.6 / NA	69	L216L (TTG→CTG)	unknown	Uncharacterized protein EC‑HemX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/36);  total (33/36)

CTGCGTTTTCCTTCCAATCGTCTAAGGAATCCGTCAACTTGTCGCTACTCGAATCTTCATCAAAATTCACATCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAACAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCTTGATTGGACAAACGTTGCACCTGTTGTTGCAATGCCGACAGGCTTTGTTCTTTGGCGGACAAATTCTGATTCAAGGCGCTGATTTGATC  >  1/1536847‑1537294                                                                                                                                                                                                                                                       |                                                                                                                                                                                                          ctGCGTTTTCCTTCCAATCGTCTAAGGAATCCGTCAACTTGTCGCTACTCGGATCTTCATCAAAATTCACATCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAg                                                                                                                                                                                                       <  1:721730/251‑1 (MQ=255)        ttccttccAATCGTCTAAGGAATCCGTCAACTTGTCGCTACTCGGATCTTCATCAAAATTCACATCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGacacac                                                                                                                                                                                                <  1:618938/251‑1 (MQ=255)         tccttccAATCGTCTAAGGAATCCGTCAACTTGTCGCTACTCGGATCTTCATCAAAATTCACATCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACt                                                                                                                                                                                               <  1:496632/251‑1 (MQ=255)                     tCTAAGGAATCCGTCAACTTGTCGCTACTCGGATCTTCATCAAAATTCACATCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATcc                                                                                                                                                                                        >  1:739616/1‑246 (MQ=255)                     tCTAAGGAATCCGTCAACTTGTCGCTACTCGGATCTTCATCAAAATTCACATCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCAcgt                                                                                                                                                                                    >  1:731325/1‑250 (MQ=255)                      cTAAGGAATCCGTCAACTTGTCGCTACTCGGATCTTCATCAAAATTCACATCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCAcgtcg                                                                                                                                                                                  <  1:861344/251‑1 (MQ=255)                            aaTCCGTCAACTTGTCGCTACTCGGATCTTCATCAAAATTCACATCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATcc                                                                                                                                                                            <  1:338331/251‑1 (MQ=255)                            aaTCCGTCAACTTGTCGCTACTCGGATCTTCATCAAAATTCACATCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATcc                                                                                                                                                                            <  1:631449/251‑1 (MQ=255)                              tCCGTCAACTTGTCGCTACTCGGATCTTCATCAAAATTCACATCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCaa                                                                                                                                                                          >  1:310208/1‑251 (MQ=255)                               ccGTCAACTTGTCGCTACTCGGATCTTCATCAAAATTCACATCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCaa                                                                                                                                                                          >  1:294495/1‑250 (MQ=255)                                 gTCAACTTGTCGCTACTCGGATCTTCATCAAAATTCACATCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAaca                                                                                                                                                                        >  1:174803/1‑250 (MQ=255)                                  tCAACTTGTCGCTACTCGGATCTTCATCAAAATTCACATCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAacac                                                                                                                                                                       <  1:147107/250‑1 (MQ=255)                                  tCAACTTGTCGCTACTCGGATCTTCATCAAAATTCACATCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAacac                                                                                                                                                                       <  1:359259/250‑1 (MQ=255)                                    aaCTTGTCGCTACTCGGATCTTCATCAAAATTCACATCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAg                                                                                                                                                                    <  1:224902/251‑1 (MQ=255)                                           cGCTACTCGGATCTTCATCAAAATTCACATCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTgcgc                                                                                                                                                             <  1:144317/251‑1 (MQ=255)                                               aCTCGGATCTTCATCAAAATTCACATCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATc                                                                                                                                                                             >  1:579145/1‑231 (MQ=255)                                                cTCGGATCTTCATCAAAATTCACATCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAg                                                                                                                                                         >  1:859701/1‑250 (MQ=255)                                                  cggaTCTTCATCAAAATTCACATCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGt                                                                                                                                                      >  1:479271/4‑251 (MQ=255)                                                          catcaAAATTCACATCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAAc                                                                                                                                              <  1:476474/251‑1 (MQ=255)                                                                      caTCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCg                                                                                                                                    >  1:189532/1‑249 (MQ=255)                                                                       aTCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAacac                                                                                                                                                                       <  1:441710/213‑1 (MQ=255)                                                                       aTCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCtt                                                                                                                                 >  1:833366/1‑251 (MQ=255)                                                                       aTCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCtt                                                                                                                                 >  1:101244/1‑251 (MQ=255)                                                                               ccGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGacac                                                                                                                                                                                                  >  1:835579/1‑178 (MQ=255)                                                                               ccGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATcc                                                                                                                                                                                        <  1:728720/188‑1 (MQ=255)                                                                               ccGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTgcgc                                                                                                                                                             <  1:418387/215‑1 (MQ=255)                                                                               ccGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGt                                                                                                                         >  1:111239/1‑251 (MQ=255)                                                                               ccGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGt                                                                                                                         <  1:47828/251‑1 (MQ=255)                                                                                  tCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAAc                                                                                                                      <  1:294737/251‑1 (MQ=255)                                                                                      ttCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGt                                                                                                                  <  1:885435/251‑1 (MQ=255)                                                                                      ttCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGt                                                                                                                  <  1:399412/251‑1 (MQ=255)                                                                                        cATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCa                                                                                                                >  1:724336/1‑251 (MQ=255)                                                                                         aTCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTAttt                                                                                                                                                 >  1:486131/1‑217 (MQ=255)                                                                                                              ggATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTgcgc                                                                                                                                                             <  1:855578/184‑1 (MQ=255)                                                                                                              ggATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCt                                                                                           <  1:69277/250‑1 (MQ=255)                                                                                                              ggATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCt                                                                                           <  1:264994/250‑1 (MQ=255)                                                                                                                  aaaTTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTc                                                                                                                            <  1:861762/213‑1 (MQ=255)                                                                                                                  aaaTTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCGGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCTTGAtt                                                                                      <  1:437239/251‑1 (MQ=255)                                                                                                                    aTTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCTTGATTgg                                                                                    >  1:310727/1‑251 (MQ=255)                                                                                                                         gCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGc                                                                                                                                   >  1:187437/1‑199 (MQ=255)                                                                                                                              atgGCGTTTTGATCCACATTGTTAAGGGACAACAATTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGCGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAGGGACACGCTGGTATCCACGTCATTATCCAACACCAATTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGCAGCCAATCATTCGGCTGCTCCGCTTTGGCTTGATTGGACAAACGTTg                                                                          >  1:543955/1‑251 (MQ=255)                                                                                                                                   aTTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCgg                                                                                                           >  1:555961/1‑213 (MQ=255)                                                                                                                                      ttGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATcc                                                                                                                                                                            <  1:176705/145‑1 (MQ=255)                                                                                                                                        ggTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATcc                                                                                                                                                                            >  1:132231/1‑143 (MQ=255)                                                                                                                                          tCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCTTGATTGGACAAACGTTGCACCTGttgttg                                                              <  1:121442/251‑1 (MQ=255)                                                                                                                                                  gTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCTTGAt                                                                                       <  1:635722/218‑1 (MQ=255)                                                                                                                                                  gTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCTTGATTGGACAAACGTTGCACCTGTTGTTGCCATGCCg                                                      >  1:277903/1‑251 (MQ=255)                                                                                                                                                  gTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCTTGATTGGACAAACGTTGCACCTGTTGTTGCAATGCCg                                                      >  1:276241/1‑251 (MQ=255)                                                                                                                                                  gTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCTTGATTGGACAAACGTTGCACCTGTTGTTGCAATGCCg                                                      >  1:28005/1‑251 (MQ=255)                                                                                                                                                  gTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCTTGATTGGACAAACGTTGCACCTGTTGTTGCAATGCCg                                                      >  1:429530/1‑251 (MQ=255)                                                                                                                                                         gACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCTTGATTGGACAAACGTTGCACCTGTTGTTGCAATGCCGACAgg                                                 >  1:436265/1‑249 (MQ=255)                                                                                                                                                              caacTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTAtt                                                                                                                                                  <  1:772636/147‑1 (MQ=255)                                                                                                                                                                   gTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACt                                                                                                                                                                                               >  1:774550/1‑97 (MQ=255)                                                                                                                                                                   gTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCTTGATTGGACAAACGTTGCACCTGTTGTTGCAATGCCGACAGGCTTTGTTCTTTg                                     >  1:610204/1‑251 (MQ=255)                                                                                                                                                                   gTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCTTGATTGGACAAACGTTGCACCTGTTGTTGCAATGCCGACAGGCTTTGTTCTTTg                                     >  1:775849/1‑251 (MQ=255)                                                                                                                                                                             ggCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCAGCTGCTCCGCTTTGGCTTGATTGGACAAACGTTGCACCTGTTGTTGCAATGCCGACAGGCTTTGTTCTTTGGCGGACAAAt                           >  1:455346/1‑251 (MQ=255)                                                                                                                                                                                   aaTGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCTTGATTGGACAAACGTTGCACCTGTTGTTGCAATGCCGACAGGCTTTGTTCTTTGGCGGACAAATTCTGAt                     <  1:110150/251‑1 (MQ=255)                                                                                                                                                                                    aTGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCTTGATTGGACAAACGTTGCACCTGTTGTTGCAATGCCGACAGGCTTTGTTCTTTGGCGGACAAATTCTGAtt                    <  1:260571/251‑1 (MQ=255)                                                                                                                                                                                       gcgcTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCTTGATTGGACAAACGTTGCACCTGTTGTTGCAATGCCGACAGGCTTTGTTCTTTGGCGGACAAATTCTGATTCaa                 >  1:656903/1‑251 (MQ=255)                                                                                                                                                                                        cgcTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCTTGATTGGACAAACGTTGCACCTGTTGTTGCAATGCCGACAGGCTTTGTTCTTTGGCGGACAAATTCTGATTCAAg                >  1:735764/1‑251 (MQ=255)                                                                                                                                                                                         gcTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGt                                                                                                                  <  1:299429/152‑1 (MQ=255)                                                                                                                                                                                         gcTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCTTGATTGGACAAACGTTGCACCTGTTGTTGCAATGCCGACAGGCTTTGTTCTTTGGCGGACAAATTCTGATTc                   >  1:604789/1‑247 (MQ=255)                                                                                                                                                                                               cACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTgg                                                                                                                                                                                             >  1:511330/1‑71 (MQ=255)                                                                                                                                                                                               cACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTc                                                                                                                 <  1:638981/147‑1 (MQ=255)                                                                                                                                                                                                 ccTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCTTGATTGGACAAACGTTGCACCTGTTGTTGCAATGCCGACAGGCTTTGTTCTTTGGCGGACAAATTCTGATTCAAGGCGCTGAtt       <  1:796717/251‑1 (MQ=255)                                                                                                                                                                                                       ccACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCTTGATTGGACAAACGTTGCACCTGTTGTTGCAATGCCGACAGGCTTTGTTCTTTGGCGGACAAATTCTGATTCAAGGCGCTGATTTGATc  <  1:474553/250‑1 (MQ=255)                                                                                                                                                                                                         aCTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTAt                                                                                                                                                                                          <  1:893108/64‑1 (MQ=255)                                                                                                                                                                                                         aCTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCtt                                                                                                <  1:62699/154‑1 (MQ=255)                                                                                                                                                                                                                                                aCTTTCAGCAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCTTGATTGGACAAACGTTGCACCTGTTGTTGCAATGCCGACAGGCTTTGTTCTTTGGCGGACAAATTc                         <  1:328662/186‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                          CTGCGTTTTCCTTCCAATCGTCTAAGGAATCCGTCAACTTGTCGCTACTCGAATCTTCATCAAAATTCACATCCAGCACCGTCAATTCATCTACGTTATTCGCCAGCTGGGATAAATTCTGCATGATGGCATTTTGGTCCACATTGTTCAGGGACAACAACTGTTTTAAATCGGCGTTAATGGCGCTACGCACCTGCGCCACTTGTGGCATGGCGACTTTGGAAAGGGTTTCATCGGCAACTTTCAACAAGGACACACTGGTATCCACGTCGTTATCCAACACCAGTTTGCGCAAAGCGTTATTTAACAGAAAATCCGCTTCGGTCAGTAACCAGTCATTCGGCTGCTCCGCTTTGGCTTGATTGGACAAACGTTGCACCTGTTGTTGCAATGCCGACAGGCTTTGTTCTTTGGCGGACAAATTCTGATTCAAGGCGCTGATTTGATC  >  1/1536847‑1537294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑