Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,523,342	A→C	S9S (TCA→TCC)	unknown →	ABC transporter, permease protein PM1327

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,523,342	0	A	C	100.0%	184.4 / NA	64	S9S (TCA→TCC)	unknown	ABC transporter, permease protein PM1327
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (35/29);  total (35/29)

CAAAGTCGGAAATTTATGATTTAATGCGCCAATTTACCGAAAGCGGCGGCACTATCGTGATGGTATCGTCGGATTTACCGGAATTAATCACCATTTCCGATCGCATCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCAAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTATTGACTTATCGGTCGGCTCTATCGTCGCATTGAGCGCTGTTTGTTTGGGGTTATTCACCCATAGGGGAGTGGCTATTC  >  1/1523101‑1523580                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               cAAAGTCGGAAATTTATGATTTAATGCGCCAATTTACCGAAAGCGGCGGCACTATCGTGATGGTATCGTCGGATTTACCGGAATTAATCACCATTTCCGATCGCATCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGc                                                                                                                                                                                                                                       <  1:376841/251‑1 (MQ=255)              ttATGATTTAATGCGCCAATTTACCGAAAGCGGCGGCACTATCGTGATGGTATCGTCGGATTTACCGGAATTAATCACCATTTCCGATCGCATCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGttt                                                                                                                                                                                                                          <  1:233519/251‑1 (MQ=255)                                                    cTATCGTGATGGTATCGTCGGATTTACCGGAATTAATCACCATTTCCGATCGCATCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAgg                                                                                                                                                                                                       >  1:504996/1‑232 (MQ=255)                                                     tATCGTGATGGTATCGTCGGATTTACCGGAATTAATCACCATTTCCGATCGCATCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAg                                                                                                                                                                                   <  1:242832/251‑1 (MQ=255)                                                      aTCGTGATGGTATCGTCGGATTTACCGGAATTAATCACCATTTCCGATCGCATCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGttt                                                                                                                                                                                                                          <  1:597593/211‑1 (MQ=255)                                                      aTCGTGATGGTATCGTCGGATTTACCGGAATTAATCACCATTTCCGATCGCATCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAg                                                                                                                                                                                   >  1:186382/1‑250 (MQ=255)                                                      aTCGTGATGGTATCGTCGGATTTACCGGAATTAATCACCATTTCCGATCGCATCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAg                                                                                                                                                                                   >  1:189543/1‑250 (MQ=255)                                                      aTCGTGATGGTATCGTCGGATTTACCGGAATTAATCACCATTTCCGATCGCATCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAg                                                                                                                                                                                   >  1:885479/1‑250 (MQ=255)                                                               gTATCGTCGGATTTACCGGAATTAATCACCATTTCCGATCGCATCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAGAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTAt                                                                                                                                                                          >  1:448176/1‑250 (MQ=255)                                                               gTATCGTCGGATTTACCGGAATTAATCACCATTTCCGATCGCATCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTAt                                                                                                                                                                          <  1:617224/250‑1 (MQ=255)                                                               gTATCGTCGGATTTACCGGAATTAATCACCATTTCCGATCGCATCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTAt                                                                                                                                                                          <  1:856579/250‑1 (MQ=255)                                                               gTATCGTCGGATTTACCGGAATTAATCACCATTTCCGATCGCATCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTAt                                                                                                                                                                          <  1:32420/250‑1 (MQ=255)                                                                  tcgtcgGATTTACCGGAATTAATCACCATTTCCGATCGCATCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGAc                                                                                                                                                                      <  1:315164/251‑1 (MQ=255)                                                                                  aaTTAATCACCATTTCCGATCGCATCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTATATCGACTAACAATATTTTTAat                                                                                                                                                      <  1:553876/251‑1 (MQ=255)                                                                                            cATTTCCGATCGCATCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTAc                                                                                                                                            <  1:67979/251‑1 (MQ=255)                                                                                              tttCCGATCGCATCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACaa                                                                                                                                          <  1:892983/251‑1 (MQ=255)                                                                                                      cGCATCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGcc                                                                                                                                                                                 >  1:257271/1‑204 (MQ=255)                                                                                                        cATCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAg                                                                                                                                                                                   >  1:850414/1‑200 (MQ=255)                                                                                                        cATCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTAt                                                                                                                                                                          <  1:759300/209‑1 (MQ=255)                                                                                                          tCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAg                                                                                                                                                                                      <  1:665314/195‑1 (MQ=255)                                                                                                            gtagtaATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAAtt                                                                                                                                                                                                                                           >  1:594010/1‑140 (MQ=255)                                                                                                            gtagtaATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTg                                                                                                                            >  1:421214/1‑251 (MQ=255)                                                                                                             tagtaATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGaa                                                                                                                                                                                                     <  1:228741/177‑1 (MQ=255)                                                                                                               gtaATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATAttt                                                                                                                                                           >  1:750801/1‑217 (MQ=255)                                                                                                                                  aGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTAttt                                                                                                                                                                                                                     <  1:515523/140‑1 (MQ=255)                                                                                                                                   gATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACtt                                                                                                     >  1:889710/1‑251 (MQ=255)                                                                                                                                     tAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCgg                                                                                                             <  1:518575/241‑1 (MQ=255)                                                                                                                                           aaaTCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCa                                                                                               <  1:90378/249‑1 (MQ=255)                                                                                                                                              tCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCa                                                                                                           >  1:689588/1‑234 (MQ=255)                                                                                                                                               cACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTaa                                                                                         >  1:637465/1‑251 (MQ=255)                                                                                                                                                 ccGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATc                                                                                                                                                                         <  1:366982/169‑1 (MQ=255)                                                                                                                                                        cACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTa                                                                                >  1:503294/1‑251 (MQ=255)                                                                                                                                                                       agaaAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATACTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTATTGACTTAtcggtcg                                                                 >  1:120633/1‑251 (MQ=255)                                                                                                                                                                          aaaTTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGAtct                                                                                                                    >  1:35955/1‑197 (MQ=255)                                                                                                                                                                                    cACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTAc                                                                                                                                            >  1:576523/1‑163 (MQ=255)                                                                                                                                                                                      ccTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTATTGACTTATCGGTCGGCTCTATCGTCGCAt                                                  >  1:383278/1‑251 (MQ=255)                                                                                                                                                                                        tgatgaTAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTATTGACTTATCGGTCGGCTCTATCGTCGCATTg                                                <  1:777213/251‑1 (MQ=255)                                                                                                                                                                                         gatgatAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATcc                                                                                                                                                  >  1:588153/1‑152 (MQ=255)                                                                                                                                                                                         gatgatAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTATTGACTTATCGGTCGGCTCTATCGTCGCATTGa                                               >  1:76696/1‑251 (MQ=255)                                                                                                                                                                                         gatgatAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTATTGACTTATCGGTCGGCTCTATCGTCGCATTGa                                               >  1:395269/1‑251 (MQ=255)                                                                                                                                                                                         gatgatAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTATTGACTTATCGGTCGGCTCTATCGTCGCATTGa                                               >  1:459383/1‑251 (MQ=255)                                                                                                                                                                                         gatgatAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTATTGACTTATCGGTCGGCTCTATCGTCGCATTGa                                               >  1:420965/1‑251 (MQ=255)                                                                                                                                                                                           tgatAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAg                                                                                                                                         <  1:303035/159‑1 (MQ=255)                                                                                                                                                                                           tgatAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTc                                                                                                >  1:758777/1‑200 (MQ=255)                                                                                                                                                                                              tAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGt                                                                                                                                        >  1:435065/1‑157 (MQ=255)                                                                                                                                                                                                   gtTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTAt                                                                                                                                                                          <  1:898270/118‑1 (MQ=255)                                                                                                                                                                                                        gAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAg                                                                                                                                         >  1:164314/1‑146 (MQ=255)                                                                                                                                                                                                        gAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTATTGACTTATCGGTCGGCTCTAtcg                                                        >  1:808597/1‑227 (MQ=255)                                                                                                                                                                                                        gAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTATTGACTTATCGGTCGGCTCTATCGTCGCATTGAGCGCTGTTTGTTTgg                                >  1:474720/1‑251 (MQ=255)                                                                                                                                                                                                           tAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTATTGACTTATCGGTCGGCTCTATCGTCGCATTGAGCGCTGTTTGTTTGGGGt                             <  1:530229/251‑1 (MQ=255)                                                                                                                                                                                                           tAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTATTGACTTATCGGTCGGCTCTATCGTCGCATTGAGCGCTGTTTGTTTGGGGt                             <  1:700600/251‑1 (MQ=255)                                                                                                                                                                                                            aaGGATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTATTGACTTATCGGTCGGCTCTATCGTCGCATTGAGCGCTGTTTGTTTGGGGtt                            >  1:866816/1‑251 (MQ=255)                                                                                                                                                                                                              ggATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACa                                                                                                                                           >  1:472548/1‑138 (MQ=255)                                                                                                                                                                                                              ggATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCTTTCTAACCGGAGGAATTGACTTATCGGTCGGCTCTATCTTCGCATTGAGCGCTGTTTGTTTGGGGTTAt                          >  1:141091/1‑251 (MQ=255)                                                                                                                                                                                                              ggATGACACTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTATTGACTTATCGGTCGGCTCTAtcgtcg                                                     <  1:602704/224‑1 (MQ=255)                                                                                                                                                                                                                   acacTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTATTGACTTATCGGTCGGCTCTATCGTCGCATTGAGCGCTGTTTGTTTGGGGTTATTCAc                      >  1:830419/1‑250 (MQ=255)                                                                                                                                                                                                                    cacTATGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTATTGACTTATCGGTCGGCTCTATCGTCGCATTGAGCGCTGTTTGTTTGGGGTTATTCAccc                    <  1:341619/251‑1 (MQ=255)                                                                                                                                                                                                                        aTGTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGAc                                                                                                       >  1:103313/1‑164 (MQ=255)                                                                                                                                                                                                                          gTTTTCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTc                                                                                            >  1:518848/1‑173 (MQ=255)                                                                                                                                                                                                                           ttttCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTATTGACTTATCGGTCGGCTCTATCGTCGCATTGAGCGCTGTTTGTTTGGGGTTATTCACCCATAggg              <  1:381699/250‑1 (MQ=255)                                                                                                                                                                                                                           ttttCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTATTGACTTATCGGTCGGCTCTATCGTCGCATTGAGCGCTGTTCGTTTGGGGTTATTCACCCATAggg              <  1:232300/250‑1 (MQ=255)                                                                                                                                                                                                                             ttCATTGAAAAAAATAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTATTGACTTATCGGTCGGCTCTATCGTCGCATTGAGCGCTGTTTGTTTGGGGTTATTCACCCATAGGGGAg           <  1:616239/251‑1 (MQ=255)                                                                                                                                                                                                                                      aaaaaTAATCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTc                                                                                                <  1:728401/157‑1 (MQ=255)                                                                                                                                                                                                                                          ataatCTCCAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTATTGACTTATCGGTCGGCTCTATCGTCGCATTGAGCGCTGTTTGTTTGGGGTTATTCACCCATAGGGGAGTGGCTATTc  >  1:774792/1‑247 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               CAAAGTCGGAAATTTATGATTTAATGCGCCAATTTACCGAAAGCGGCGGCACTATCGTGATGGTATCGTCGGATTTACCGGAATTAATCACCATTTCCGATCGCATCGTAGTAATGCGTAACGGTGAAAAGATAAAAGAAATCACCGATCGCACCGAAATTACGGAAGAAAATTTAATGCACCTGATGATAGGTGTTTAGAATAAGGATGACACTATGTTTTCATTGAAAAAAATAATCTCAAAATTAGGCATCGGGTTAGTTTTATTTTTCATGATTTTAGGAATGTCTTTCACATCAGAAGCCTTTTTATCGACTAACAATATTTTTAATATCCTATTACAAGTATCCGTCATTTGCGTGATCTCCGTCGGCATGACTTACGTCATTCTAACCGGAGGTATTGACTTATCGGTCGGCTCTATCGTCGCATTGAGCGCTGTTTGTTTGGGGTTATTCACCCATAGGGGAGTGGCTATTC  >  1/1523101‑1523580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑