Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	294,020	C→T	S8S (AGC→AGT)	unknown →	Thioredoxin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	294,020	0	C	T	100.0%	214.7 / NA	79	S8S (AGC→AGT)	unknown	Thioredoxin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (51/28);  total (51/28)

TTAACAGGCCATCAGGCATTTCTAACGGAAGAGGCGCTAACCAGCATTGCCGATGTTACCCTGTCTAATATTTATGCTATTGGGAAAGGCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGCGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGGCGTGCGTAGTGTCCCGACACTATTATTATTTAAAGATGGCAAACCGGTAGCAACC  >  1/293775‑294263                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    ttaACAGGCCATCAGGCATTTCTAACGGAAGAGGCGCTAACCAGCATTGCCGATGTTACCCTGTCTAATATTTATGCTATTGGGAAAGGCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGc                                                                                                                                                                                                                                                <  1:595460/251‑1 (MQ=255)                                  gcgcTAACCAGCATTGCCGATGTTACCCTGTCTAATATTTATGCTATTGGGAAAGGCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCagag                                                                                                                                                                                                                 <  1:2128/249‑1 (MQ=255)                                            gCATTGCCGATGTTACCCTGTCTAATATTTATGCTATTGGGAAAGGCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGtatta                                                                                                                                                                                                     >  1:867209/1‑251 (MQ=255)                                                tGCCGATGTTACCCTGTCTAATATTTATGCTATTGGGAAAGGCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGGATTTCATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAg                                                                                                                                                                                                 <  1:550634/251‑1 (MQ=255)                                                     atgctACCCTGTCTAATATTTATGCTATTGGGAAAGGCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACttt                                                                                                                                                                                            >  1:602810/5‑251 (MQ=255)                                                          aCCCTGTCTAATATTTATGCTATTGGGAAAGGCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAAt                                                                                                                                                                                                                       >  1:35271/1‑219 (MQ=255)                                                               gTCTAATATTTATGCTATTGGGAAAGGCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACAt                                                                                                                                                                                  >  1:870243/1‑251 (MQ=255)                                                               gTCTAATATTTATGCTATTGGGAAAGGCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACAt                                                                                                                                                                                  >  1:850470/1‑251 (MQ=255)                                                               gTCTAATATTTATGCTATTGGGAAAGGCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACAt                                                                                                                                                                                  >  1:554224/1‑251 (MQ=255)                                                               gTCTAATATTTATGCTATTGGGAAAGGCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACAt                                                                                                                                                                                  >  1:512675/1‑251 (MQ=255)                                                               gTCTAATATTTATGCTATTGGGAAAGGCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGAAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACAt                                                                                                                                                                                  >  1:888025/1‑251 (MQ=255)                                                                tCTAATATTTATGCTATTGGGAAAGGCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACAt                                                                                                                                                                                  <  1:395648/250‑1 (MQ=255)                                                                    atatTTATGCTATTGGGAAAGGCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGtat                                                                                                                                                                                                       >  1:137683/1‑225 (MQ=255)                                                                        ttATGCTATTGGGAAAGGCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGAc                                                                                                                                                                         <  1:581121/251‑1 (MQ=255)                                                                        ttATGCTATTGGGAAAGGCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGAc                                                                                                                                                                         <  1:822613/251‑1 (MQ=255)                                                                         tATGCTATTGGGAAAGGCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGAcc                                                                                                                                                                        <  1:786662/251‑1 (MQ=255)                                                                                  gggAAAGGCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAAt                                                                                                                                                               >  1:603717/1‑251 (MQ=255)                                                                                       aGGCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTg                                                                                                                                                          >  1:44978/1‑251 (MQ=255)                                                                                        ggCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTg                                                                                                                                                          >  1:17135/1‑250 (MQ=255)                                                                                        ggCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTg                                                                                                                                                          >  1:855270/1‑250 (MQ=255)                                                                                              ccTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGtat                                                                                                                                                                                                       >  1:519056/1‑199 (MQ=255)                                                                                                                    cttcttAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGaa                                                                                                                             >  1:558553/1‑251 (MQ=255)                                                                                                                                             aatTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAAt                                                                                                         >  1:130918/1‑246 (MQ=255)                                                                                                                                             aatTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGa                                                                                                    >  1:358883/1‑251 (MQ=255)                                                                                                                                                    gtTATTCGCATAGCGCTATGCTACTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGa                                                                                                    <  1:396859/244‑1 (MQ=255)                                                                                                                                                           gCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTAttatta                                                                                                                                                                                                  <  1:703380/143‑1 (MQ=255)                                                                                                                                                           gCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGa                                                                                       >  1:287031/1‑250 (MQ=255)                                                                                                                                                                gcgcTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAAt                                                                                                                                                                                                                       >  1:506118/1‑117 (MQ=255)                                                                                                                                                                gcgcTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATaa                                                                                 >  1:614669/1‑251 (MQ=255)                                                                                                                                                                gcgcTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATaa                                                                                 >  1:163819/1‑251 (MQ=255)                                                                                                                                                                gcgcTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATaa                                                                                 >  1:530749/1‑251 (MQ=255)                                                                                                                                                                gcgcTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATaa                                                                                 >  1:537783/1‑251 (MQ=255)                                                                                                                                                                  gctatgctatTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGAc                                                                                                                                                                         >  1:276044/1‑161 (MQ=255)                                                                                                                                                                  gctatgctatTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAAc                                                                                >  1:359971/1‑250 (MQ=255)                                                                                                                                                                   ctatgctatTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGtattat                                                                                                                                                                                                    >  1:737294/1‑133 (MQ=255)                                                                                                                                                                     atgctatTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTAttatta                                                                                                                                                                                                  >  1:191327/1‑133 (MQ=255)                                                                                                                                                                     atgctatTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGccc                                                                                                                                                       <  1:850160/176‑1 (MQ=255)                                                                                                                                                                     atgctatTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAatat                                                                                          >  1:540048/1‑237 (MQ=255)                                                                                                                                                                     atgctatTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAgg                                                                            >  1:866088/1‑251 (MQ=255)                                                                                                                                                                     atgctatTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAgg                                                                            >  1:361128/1‑251 (MQ=255)                                                                                                                                                                     atgctatTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAgg                                                                            >  1:146399/1‑251 (MQ=255)                                                                                                                                                                      tgctatTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAACTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGc                                                                           <  1:650256/251‑1 (MQ=255)                                                                                                                                                                        ctatTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTtgat                                                                                                                                            >  1:709715/1‑184 (MQ=255)                                                                                                                                                                        ctatTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAatat                                                                                          <  1:553184/234‑1 (MQ=255)                                                                                                                                                                          attatGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGTAAAATGATTGCCCCGATCCTtga                                                                                                                                             >  1:550830/1‑181 (MQ=255)                                                                                                                                                                          attatGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAatat                                                                                          >  1:431924/1‑232 (MQ=255)                                                                                                                                                                               gcgcAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTg                                                                   >  1:697665/1‑250 (MQ=255)                                                                                                                                                                               gcgcAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTg                                                                   >  1:315670/1‑250 (MQ=255)                                                                                                                                                                                  cAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGcac                                                                <  1:284676/250‑1 (MQ=255)                                                                                                                                                                                  cAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGcac                                                                <  1:413990/250‑1 (MQ=255)                                                                                                                                                                                        aTTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCgg                                                         <  1:622711/251‑1 (MQ=255)                                                                                                                                                                                              cacTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGgcgtgcg                                                   >  1:787015/1‑251 (MQ=255)                                                                                                                                                                                                cTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGAc                                                                                                                                                                                               >  1:208265/1‑109 (MQ=255)                                                                                                                                                                                                cTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGGCGTGCGTa                                                 >  1:475124/1‑251 (MQ=255)                                                                                                                                                                                                cTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGGCGTGCGTa                                                 >  1:593353/1‑251 (MQ=255)                                                                                                                                                                                                   gTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACAt                                                                                                                                                                                  >  1:668642/1‑119 (MQ=255)                                                                                                                                                                                                   gTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGcac                                                                <  1:419001/233‑1 (MQ=255)                                                                                                                                                                                                   gTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGGCGTGCGTAgtg                                              >  1:807914/1‑251 (MQ=255)                                                                                                                                                                                                      cacaACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGGCGTGCGTAGTGTcc                                           <  1:216245/251‑1 (MQ=255)                                                                                                                                                                                                          acaGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGGCGTGCGTAGTGTCCCGa                                        >  1:51200/1‑250 (MQ=255)                                                                                                                                                                                                             gTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGGCGTGCGTAGTGTCCCGaca                                      >  1:892091/1‑249 (MQ=255)                                                                                                                                                                                                             gTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGGCGTGCGTAGTGTCCCGa                                        >  1:466179/1‑247 (MQ=255)                                                                                                                                                                                                              tAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGGCGTGCGTAGTGTCCCGac                                       <  1:143779/247‑1 (MQ=255)                                                                                                                                                                                                               aaCAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTgatgat                                                                                                                                         <  1:754599/148‑1 (MQ=255)                                                                                                                                                                                                                 cAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGGCGTGCGTAGTGTCCCGACACtatta                                <  1:278262/251‑1 (MQ=255)                                                                                                                                                                                                                 cAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGGCGTGCGTAGTGTCCCGACACtatta                                <  1:517053/251‑1 (MQ=255)                                                                                                                                                                                                                    aaGGAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGGCGTGCGTAGTGTCCCGACACtattatta                             <  1:644901/251‑1 (MQ=255)                                                                                                                                                                                                                       gAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGGCGTGCGTAGTGTCCCGACACtattattat                            <  1:228893/249‑1 (MQ=255)                                                                                                                                                                                                                       gAAAAAAAATGAGCGAAGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGGCGTGCGTAGTGTCCCGACACTattattatt                           <  1:366520/250‑1 (MQ=255)                                                                                                                                                                                                                                      aaGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGgcgtg                                                     >  1:595723/1‑209 (MQ=255)                                                                                                                                                                                                                                      aaGTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGGCGTGCGTAGTGTCCCGACACTATTATTATTTAAAGATGGCAAACCg           >  1:781672/1‑251 (MQ=255)                                                                                                                                                                                                                                        gTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAAc                                                                                <  1:589045/180‑1 (MQ=255)                                                                                                                                                                                                                                        gTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGcac                                                                >  1:726784/1‑196 (MQ=255)                                                                                                                                                                                                                                        gTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGcac                                                                >  1:552026/1‑196 (MQ=255)                                                                                                                                                                                                                                        gTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGcac                                                                <  1:694721/196‑1 (MQ=255)                                                                                                                                                                                                                                        gTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGgcgt                                                      >  1:33985/1‑206 (MQ=255)                                                                                                                                                                                                                                        gTATTACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGGCGTGCGTAGTGTCCCGACACtattatta                             >  1:533714/1‑231 (MQ=255)                                                                                                                                                                                                                                           ttACATTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGGCGTGCGTAGTGTCCCGACACTATTATTATTTAAAGATGGCAAACCGGTAGc      <  1:685032/251‑1 (MQ=255)                                                                                                                                                                                                                                               aTTCCAGTGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGGCGTGCGTAGTGTCCCGACACTATTATTATTTAAAGATGGCAAACCGGTAGCAAcc  <  1:747432/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    TTAACAGGCCATCAGGCATTTCTAACGGAAGAGGCGCTAACCAGCATTGCCGATGTTACCCTGTCTAATATTTATGCTATTGGGAAAGGCAAACCTTGCGATAATGTAGTTTTGTCTTCTTAGCTTTTAACAATTAAAATAATTTGTGTTATTCGCATAGCGCTATGCTATTATGCGCAGGAAATTAAACACTAGTACACAACAGTAACAAAAGGAAAAAAAATGAGCGAAGTATTACATTCCAGCGATGCAACATTCGTTGCAGACGTTTTAAATTCAGAGGTCCCTGTATTATTAGACTTTTGGGCTACATGGTGCGGACCGTGCAAAATGATTGCCCCGATCCTTGATGATTTGGCGGTGGAATTTGATGGTAAAGTGAAAATTGTGAAAATCAATATTGACGATAACCAGGCCACCCCTGCACAGTTCGGCGTGCGTAGTGTCCCGACACTATTATTATTTAAAGATGGCAAACCGGTAGCAACC  >  1/293775‑294263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑