Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,490,042	G→A	noncoding (46/77 nt)	unknown →	tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,490,042	0	G	A	100.0%	119.1 / NA	43	noncoding (46/77 nt)	unknown	tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/17);  total (26/17)

AAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCGATATTTCCCTATAATCGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTGGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGATTCATTTTAGTTTTACCTTTAGTACCTGATATAGTACCCTATCAGGGGAATTTAGTGATTTTCTAGGTACTAAAAATAGGCATAGTAGACAAAAGAGTTGGTTT  >  1/1489800‑1490278                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             aaaaaaTGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAgg                                                                                                                                                                                                                                      >  1:219756/1‑251 (MQ=255)                  caAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTgggg                                                                                                                                                                                                                     <  1:380427/251‑1 (MQ=255)                    aaaGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGc                                                                                                                                                                                                                   <  1:675726/251‑1 (MQ=255)                     aaGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCa                                                                                                                                                                                                                  >  1:608237/1‑251 (MQ=255)                               gCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATAtt                                                                                                                                                                                                        >  1:285904/1‑251 (MQ=255)                                cTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACatat                                                                                                                                                                                                         <  1:543590/249‑1 (MQ=255)                                             gAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATcc                                                                                                                                                                                                   >  1:518454/1‑242 (MQ=255)                                             gAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACtt                                                                                                                                                                                          >  1:358379/1‑251 (MQ=255)                                                  acCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCAct                                                                                                                                                                                      >  1:338876/1‑250 (MQ=255)                                                                 gggCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACtt                                                                                                                                                                                          <  1:234240/231‑1 (MQ=255)                                                                                                          ggTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAg                                                                                                                             >  1:650596/1‑251 (MQ=255)                                                                                                                          cTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATg                                                                                                                    <  1:833255/244‑1 (MQ=255)                                                                                                                            ttAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGtt                                                                                                                                                                                                                         >  1:294625/1‑141 (MQ=255)                                                                                                                              aaaTAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTg                                                                                                                                                                                                                        <  1:810445/140‑1 (MQ=255)                                                                                                                                aTAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGAtt                                                                                                        >  1:294150/1‑250 (MQ=255)                                                                                                                                   acacATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGATTCAtt                                                                                                    >  1:755643/1‑251 (MQ=255)                                                                                                                                     acaTAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATcc                                                                                                                                                                                                   >  1:33021/1‑154 (MQ=255)                                                                                                                                      caTAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCActct                                                                                                                                                                                    >  1:378896/1‑168 (MQ=255)                                                                                                                                      caTAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAg                                                                                                                             <  1:469927/223‑1 (MQ=255)                                                                                                                                          atatTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCaaacc                                                                                                                                                                     >  1:362098/1‑179 (MQ=255)                                                                                                                                            atTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTcac                                                                                                                                                                                                                                         <  1:566210/109‑1 (MQ=255)                                                                                                                                            atTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGt                                                                                                                                                                                                                               >  1:253778/1‑119 (MQ=255)                                                                                                                                            atTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAAt                                                                                                                                                     <  1:79931/193‑1 (MQ=255)                                                                                                                                            atTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGATTCATTTTAGtt                                                                                              >  1:289762/1‑248 (MQ=255)                                                                                                                                              tCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAgtgt                                                                                                                          >  1:128652/1‑218 (MQ=255)                                                                                                                                                         ccccAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTcc                                                                                                                                 <  1:335711/200‑1 (MQ=255)                                                                                                                                                           ccaatatTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGATTCATTTTAGTTTTACCTTTAGTACCTGat                                                                            <  1:386206/248‑1 (MQ=255)                                                                                                                                                           ccaatatTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGATTCATTTTAGTTTTACCTTTAGTACCTGat                                                                            <  1:701487/248‑1 (MQ=255)                                                                                                                                                              atatTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATc                                                                                                                                       >  1:152740/1‑189 (MQ=255)                                                                                                                                                                  ttCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGATTCATTTTAGTTTTACCTTTAGTACCTGATATAGTAc                                                                     <  1:486240/251‑1 (MQ=255)                                                                                                                                                                    cccTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGATTCATTTTAGTTTTACCTTTAGTACCTGATATAGTAccc                                                                   <  1:699306/251‑1 (MQ=255)                                                                                                                                                                     ccTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCActc                                                                                                                                                                                     <  1:667713/136‑1 (MQ=255)                                                                                                                                                                                 ccTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTggggg                                                                                                                                                                                                                    >  1:677948/1‑93 (MQ=255)                                                                                                                                                                                    cggaatgtgtataagagacagcccATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGATTCATTTTAGTTTTACCTTTAGTACCTGATATAGTACCCTATCAGGGGAATTTAg                                                   <  1:604938/230‑1 (MQ=255)                                                                                                                                                                                      aTTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCAtact                                                                                                                                                                                              <  1:691150/110‑1 (MQ=255)                                                                                                                                                                                      aTTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGc                                                                                                                   >  1:555756/1‑185 (MQ=255)                                                                                                                                                                                           aCGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATaaa                                                                                                                                                      >  1:748411/1‑145 (MQ=255)                                                                                                                                                                                             gCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGATTCATTTTAGTTTTACCTTTAGTACCTGATATAGTAccc                                                                   >  1:199024/1‑226 (MQ=255)                                                                                                                                                                                                cacaCCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAg                                                                                                                             >  1:105150/1‑165 (MQ=255)                                                                                                                                                                                                       cccccATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCActc                                                                                                                                                                                     >  1:284114/1‑102 (MQ=255)                                                                                                                                                                                                       cccccATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGATTCATTTTAGTTTTACCTTTAGTACCTGATATAGTACCCTATCAGGGGAATTTAGTGATTTTCTAGGTACTaaa                                >  1:363407/1‑251 (MQ=255)                                                                                                                                                                                                             tAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCAtact                                                                                                                                                                                              >  1:310222/1‑87 (MQ=255)                                                                                                                                                                                                                                     aCTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGATTCATTTTAGTTTTACCTTTAGTACCTGATATAGTACCCTATCAGGGGAATTTAGTGATTTTCTAGGTACTAAAAATAGGCATAGTAGACAAAAGAGTTGGttt  >  1:293933/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             AAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCGATATTTCCCTATAATCGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTGGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGATTCATTTTAGTTTTACCTTTAGTACCTGATATAGTACCCTATCAGGGGAATTTAGTGATTTTCTAGGTACTAAAAATAGGCATAGTAGACAAAAGAGTTGGTTT  >  1/1489800‑1490278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑