Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,489,972	C→G	intergenic (+245/‑25)	unknown → / → unknown	Protein TadG, associated with Flp pilus assembly/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,489,972	0	C	G	100.0%	154.1 / NA	54	intergenic (+245/‑25)	unknown/unknown	Protein TadG, associated with Flp pilus assembly/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (29/25);  total (29/25)

GTATGTAAATAATAAAATTAAAACCGTCTTTTACTCAAAAAGACGGTTTTCTTTTACCCTACATACGATCAAAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCGATATTTCCCTATAATCGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTGGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGATTCATTTTAGTTTTACCTTTAGTACCTGATATAGTACCC  >  1/1489729‑1490213                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  gTATGTAAATAATAAAATTAAAACCGTCTTTTACTCAAAAAGACGGTTTTCTTTTACCCTACATACGATCAAAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCtt                                                                                                                                                                                                                                            >  1:765346/1‑251 (MQ=255)                 aTTAAAACCGTCTTTTACTCAAAAAGACGGTTTTCTTTTACCCTACATACGATCAAAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCacac                                                                                                                                                                                                                            <  1:221319/251‑1 (MQ=255)                             ttttACTCAAAAAGACGGTTTTCTTTTACCCTACATACGATCAAAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGc                                                                                                                                                                                                                <  1:113979/251‑1 (MQ=255)                                    cAAAAAGACGGTTTTCTTTTACCCTACATACGATCAAAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCg                                                                                                                                                                                                         >  1:714419/1‑251 (MQ=255)                                                   cTTTTACCCTACATACGATCAAAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCt                                                                                                                                                                                                               <  1:117518/230‑1 (MQ=255)                                                   cTTTTACCCTACATACGATCAAAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGt                                                                                                                                                                                                           <  1:241016/234‑1 (MQ=255)                                                     tttACCCTACATACGATCAAAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTc                                                                                                                                                                                        <  1:164447/251‑1 (MQ=255)                                                        aCCCTACATACGATCAAAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTc                                                                                                                                                                                                                                       >  1:71924/1‑201 (MQ=255)                                                        aCCCTACATACGATCAAAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATa                                                                                                                                                                                     >  1:855757/1‑251 (MQ=255)                                                        aCCCTACATACGATCAAAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATa                                                                                                                                                                                     >  1:565776/1‑251 (MQ=255)                                                         cccTACATACGATCAAAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATaa                                                                                                                                                                                    >  1:213624/1‑251 (MQ=255)                                                         cccTACATACGATCAAAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATaa                                                                                                                                                                                    >  1:523749/1‑251 (MQ=255)                                                         cccTACATACGATCAAAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATaa                                                                                                                                                                                    >  1:348434/1‑251 (MQ=255)                                                          ccTACATACGATCAAAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCAt                                                                                                                                                                                                                   >  1:608795/1‑219 (MQ=255)                                                          ccTACATACGATCAAAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAAt                                                                                                                                                                                   <  1:739675/251‑1 (MQ=255)                                                             aCATACGATCAAAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGc                                                                                                                                                                                                                                  <  1:591553/201‑1 (MQ=255)                                                             aCATACGATCAAAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAAt                                                                                                                                                                                   <  1:23150/248‑1 (MQ=255)                                                                 aCGATCAAAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGAttag                                                                                                                                                                            <  1:174865/251‑3 (MQ=255)                                                                        aaaaaaTGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAgg                                                                                                                                                                     >  1:219756/1‑251 (MQ=255)                                                                                    catcaCAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTcaca                                                                                                                                                                                                                             >  1:244660/1‑183 (MQ=255)                                                                                         caAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTgggg                                                                                                                                                    <  1:380427/251‑1 (MQ=255)                                                                                           aaaGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGc                                                                                                                                                  <  1:675726/251‑1 (MQ=255)                                                                                            aaGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCa                                                                                                                                                 >  1:608237/1‑251 (MQ=255)                                                                                                      gCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATAtt                                                                                                                                       >  1:285904/1‑251 (MQ=255)                                                                                                       cTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACatat                                                                                                                                        <  1:543590/249‑1 (MQ=255)                                                                                                                    gAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATcc                                                                                                                                  >  1:518454/1‑242 (MQ=255)                                                                                                                    gAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACtt                                                                                                                         >  1:358379/1‑251 (MQ=255)                                                                                                                         acCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCAct                                                                                                                     >  1:338876/1‑250 (MQ=255)                                                                                                                                        gggCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACtt                                                                                                                         <  1:234240/231‑1 (MQ=255)                                                                                                                                                                                 ggTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAg                                                            >  1:650596/1‑251 (MQ=255)                                                                                                                                                                                                 cTTTAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATg                                                   <  1:833255/244‑1 (MQ=255)                                                                                                                                                                                                   ttAAATAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGtt                                                                                                                                                        >  1:294625/1‑141 (MQ=255)                                                                                                                                                                                                     aaaTAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGc                                                                                                                                                                                                                                  >  1:443624/1‑65 (MQ=255)                                                                                                                                                                                                     aaaTAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTg                                                                                                                                                       <  1:810445/140‑1 (MQ=255)                                                                                                                                                                                                       aTAACACATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGAtt                                       >  1:294150/1‑250 (MQ=255)                                                                                                                                                                                                          acacATAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGATTCAtt                                   >  1:755643/1‑251 (MQ=255)                                                                                                                                                                                                            acaTAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATcc                                                                                                                                  >  1:33021/1‑154 (MQ=255)                                                                                                                                                                                                             caTAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCActct                                                                                                                   >  1:378896/1‑168 (MQ=255)                                                                                                                                                                                                             caTAATATTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAg                                                            <  1:469927/223‑1 (MQ=255)                                                                                                                                                                                                                 atatTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCaaacc                                                                                                    >  1:362098/1‑179 (MQ=255)                                                                                                                                                                                                                   atTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAAt                                                                                                                                                                                   <  1:553713/98‑1 (MQ=255)                                                                                                                                                                                                                   atTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTcac                                                                                                                                                                        <  1:566210/109‑1 (MQ=255)                                                                                                                                                                                                                   atTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGt                                                                                                                                                              >  1:253778/1‑119 (MQ=255)                                                                                                                                                                                                                   atTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAAt                                                                                    <  1:79931/193‑1 (MQ=255)                                                                                                                                                                                                                   atTCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGATTCATTTTAGtt                             >  1:289762/1‑248 (MQ=255)                                                                                                                                                                                                                     tCTGGACTTTACCCCAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAgtgt                                                         >  1:128652/1‑218 (MQ=255)                                                                                                                                                                                                                                ccccAATATTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTcc                                                                <  1:335711/200‑1 (MQ=255)                                                                                                                                                                                                                                  ccaatatTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGATTCATTTTAGTTTTACCTTTAGTACCTGat           <  1:701487/248‑1 (MQ=255)                                                                                                                                                                                                                                  ccaatatTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGATTCATTTTAGTTTTACCTTTAGTACCTGat           <  1:386206/248‑1 (MQ=255)                                                                                                                                                                                                                                     atatTTCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATc                                                                      >  1:152740/1‑189 (MQ=255)                                                                                                                                                                                                                                         ttCCCTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGATTCATTTTAGTTTTACCTTTAGTACCTGATATAGTAc    <  1:486240/251‑1 (MQ=255)                                                                                                                                                                                                                                           cccTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGATTCATTTTAGTTTTACCTTTAGTACCTGATATAGTAccc  <  1:699306/251‑1 (MQ=255)                                                                                                                                                                                                                                            ccTATAATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTAGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCActc                                                                                                                    <  1:667713/136‑1 (MQ=255)                                                                                                                                                                                                                                               ataATGGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAAt                                                                                                                                                                                   >  1:237263/1‑70 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  GTATGTAAATAATAAAATTAAAACCGTCTTTTACTCAAAAAGACGGTTTTCTTTTACCCTACATACGATCAAAAAAATGACCTCATCACAAAAGCTAATCAGCTAAAATCACAAGGAACAACCTGACTTTTCAAAGGGCAAATAATTTTTCCACGTTTTTGAACATTAAAAACGCCGGTAAAAGCAACCGCACTTTAAATAACACATAATATTCTGGACTTTACCCCGATATTTCCCTATAATCGCGCCTTAATTCTACGCTCACACCGCCCCCATAGCTCAGTCGGTCAGAGCAGTCGACTCATAATCGATTGGTCACAGGTTCAAGTCCTGTTGGGGGCACCAACATATTAATCCATACTACTTCACTCTGATTCAAGTCAAACCAAAACCACTTATAAATTAACATATTTAATCATTTCCATAGTGTATTATGCTTTTTTTGATTCATTTTAGTTTTACCTTTAGTACCTGATATAGTACCC  >  1/1489729‑1490213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑