Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,487,909	A→G	S147S (TCA→TCG)	unknown →	Flp pilus assembly surface protein TadF, ATP/GTP‑binding motif

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,487,909	0	A	G	100.0%	129.9 / NA	49	S147S (TCA→TCG)	unknown	Flp pilus assembly surface protein TadF, ATP/GTP‑binding motif
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/17);  total (32/17)

ATGTTCTTCGTGAACGTAATCAACTATACAATGATAATGGGAGCAAACCACTAAAAAGTACTGATTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGCCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAGTCCTGAATCATTAACGACTCTAAGTACCTGCCAGCCTTATCGTAAGTTGGATAATAATCTCTCTTATTTGTCACCACGCTCAGAAGCAGCAAACCCTGACAACCAAAGAAAAATTCCACTGTATCAGGTAACACTTTGTGTTGAAGCAGGTAGCTTTTTTAAAAATTTAATAACACGCAAAGAAAACCAGTCATTTGGTATGCTAAGTTCCTCTTCACTGGCA  >  1/1487671‑1488059                                                                                                                                                                                                                                               |                                                                                                                                                       atattcttcGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGaa                                                                                                                                            >  1:727878/4‑251 (MQ=255)             agcGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAAcc                                                                                                                                  >  1:385261/3‑249 (MQ=255)                     cagttataCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAAcc                                                                                                                                  >  1:473794/5‑241 (MQ=255)                     cagttataCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCa                                                                                                                        >  1:297535/5‑251 (MQ=255)                     cagttataCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCa                                                                                                                        >  1:349469/5‑251 (MQ=255)                       gttataCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCaaa                                                                                                                      >  1:97614/3‑251 (MQ=255)                       gttataCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCaaa                                                                                                                      >  1:760385/3‑251 (MQ=255)                       gttataCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCaaa                                                                                                                      >  1:228923/3‑251 (MQ=255)                       gttataCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCaaa                                                                                                                      >  1:560506/3‑251 (MQ=255)                          ataCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGaa                                                                                                                   >  1:694374/1‑251 (MQ=255)                           taCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGaaa                                                                                                                  >  1:786500/1‑251 (MQ=255)                               aTGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAAt                                                                                                               >  1:707405/1‑250 (MQ=255)                                 gATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAAtcc                                                                                                             >  1:154306/1‑248 (MQ=255)                                            cgaaaaaCTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAgg                                                                                                 <  1:802435/245‑1 (MQ=255)                                            cgaaaaaCTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAgg                                                                                                 <  1:421216/245‑1 (MQ=255)                                              aaaaaCTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCGCTGTATCAGGt                                                                                                <  1:621494/246‑1 (MQ=255)                                                 aaCTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAATTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAAccc                                                                                                                                 <  1:433342/213‑1 (MQ=255)                                                  aCTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGtgaccc                                                                                           <  1:316086/251‑6 (MQ=255)                                                             cTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGt                                                                                                <  1:158633/235‑1 (MQ=255)                                                               gacttAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTc                                                                                                                                                <  1:316959/182‑1 (MQ=255)                                                               gacttAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAatcagc                                                                                                                                       <  1:842431/191‑1 (MQ=255)                                                                acttAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGc                                                                              >  1:452542/3‑250 (MQ=255)                                                                                           aaCTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAaca                                                  >  1:877908/1‑251 (MQ=255)                                                                                            aCTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAacac                                                 <  1:644617/251‑1 (MQ=255)                                                                                             cTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGt                                                                                                <  1:248754/203‑1 (MQ=255)                                                                                                  tttgtttgGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAg                                           <  1:205728/251‑1 (MQ=255)                                                                                                     gtttgGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGaaa                                        >  1:273175/1‑251 (MQ=255)                                                                                                        tgGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAAcc                                     <  1:744314/251‑1 (MQ=255)                                                                                                          gagaTAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAg                                   >  1:55804/1‑251 (MQ=255)                                                                                                            gaTAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGAc                                                                                                                             >  1:732628/1‑159 (MQ=255)                                                                                                            gaTAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAAc                                                                                                                          <  1:684249/162‑1 (MQ=255)                                                                                                            gaTAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTAt                                                                                                     <  1:266892/183‑1 (MQ=255)                                                                                                            gaTAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGt                                  >  1:840927/1‑250 (MQ=255)                                                                                                            gaTAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGt                                  >  1:30384/1‑250 (MQ=255)                                                                                                            gaTAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGt                                  >  1:869934/1‑250 (MQ=255)                                                                                                            gaTAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGt                                  >  1:888826/1‑250 (MQ=255)                                                                                                            gaTAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGCCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGt                                  >  1:852696/1‑250 (MQ=255)                                                                                                                      gtccAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGTCATTTGGTATg                       >  1:135509/3‑251 (MQ=255)                                                                                                                       tccAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGTCATTTGGTATgt                      <  1:81485/250‑2 (MQ=255)                                                                                                                          aaaTAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAg                                           >  1:138188/1‑227 (MQ=255)                                                                                                                          aaaTAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGTCATTTGGTATGTTaa                   >  1:85235/1‑251 (MQ=255)                                                                                                                              aaaGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGTCATTTGGTATGTTAAGTTc               <  1:462529/251‑1 (MQ=255)                                                                                                                                     gtgtCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGTCATTTGGTATGTTAAGTTCCTCTTc         <  1:15138/250‑1 (MQ=255)                                                                                                                                     gtgtCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGTCATTTGGTATGTTAAGTTCCTCTTCa        >  1:53332/1‑251 (MQ=255)                                                                                                                                      tgtCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGTCATTTGGTATGTTAAGTTCCTCTTCa        >  1:489688/1‑250 (MQ=255)                                                                                                                                        tCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAAccc                                                                                                                                 >  1:609688/1‑127 (MQ=255)                                                                                                                                        tCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAgg                                                                                                 >  1:465057/1‑159 (MQ=255)                                                                                                                                        tCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGTCATTTGGTATGTTAAGt                 >  1:424911/1‑239 (MQ=255)                                                                                                                                           cTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGTCATTTGGTATGTTAAGTTCCTCTTCACTAGCa  >  1:89932/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                       ATGTTCTTCGTGAACGTAATCAACTATACAATGATAATGGGAGCAAACCACTAAAAAGTACTGATTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGCCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAGTCCTGAATCATTAACGACTCTAAGTACCTGCCAGCCTTATCGTAAGTTGGATAATAATCTCTCTTATTTGTCACCACGCTCAGAAGCAGCAAACCCTGACAACCAAAGAAAAATTCCACTGTATCAGGTAACACTTTGTGTTGAAGCAGGTAGCTTTTTTAAAAATTTAATAACACGCAAAGAAAACCAGTCATTTGGTATGCTAAGTTCCTCTTCACTGGCA  >  1/1487671‑1488059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑