Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,487,836	G→A	S123N (AGT→AAT)	unknown →	Flp pilus assembly surface protein TadF, ATP/GTP‑binding motif

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,487,836	0	G	A	100.0%	210.2 / NA	73	S123N (AGT→AAT)	unknown	Flp pilus assembly surface protein TadF, ATP/GTP‑binding motif
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (48/25);  total (48/25)

AACAGACTTAGTTATTGTTCGTACGACCCAAGGGAAACTGGACAATGCCTCATACTCACTGGTCAATGTTCTTCGTGAACGTAATCAACTATACAATGATAATGGGAGCAAACCACTAAAAAGTACTGATTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGCCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAGTCCTGAATCATTAACGACTCTAAGTACCTGCCAGCCTTATCGTAAGTTGGATAATAATCTCTCTTATTTGTCACCACGCTCAGAAGCAGCAAACCCTGACAACCAAAGAAAAATTCCACTGTATCAGGTAACACTTTGTGTTGAAGCAGGTAGCTTTTTTAAAAATTTAATAACACGCAAAGAAAACCAGTCATTTGGTATGCTAAGTTCCTCTTCACTGGCA  >  1/1487606‑1488059                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                aaCAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGaaatgttaacgaatc                                                                                                                                                                                                             <  1:651093/251‑14 (MQ=255)         tAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCATTATTGAGCACTGGGAAGAGAAAAAAAATCCTGaaatgttaacgaatctaggcaca                                                                                                                                                                                                     >  1:720347/1‑230 (MQ=255)          aGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGaaatgttaacgaa                                                                                                                                                                                                               >  1:524724/1‑229 (MQ=255)                          aCCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTaa                                                                                                                                                                                    >  1:254629/1‑251 (MQ=255)                            ccagggaaaaCTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGt                                                                                                                                                                                  <  1:276791/244‑1 (MQ=255)                                   aaaCTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGatga                                                                                                                                                                           >  1:675512/1‑249 (MQ=255)                                   aaaCTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGatga                                                                                                                                                                           >  1:18778/1‑249 (MQ=255)                                               gCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTa                                                                                                                                                               >  1:794139/1‑251 (MQ=255)                                                   cgtattCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGaaa                                                                                                                                                                                                                         >  1:126619/6‑188 (MQ=255)                                                              gTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAatctatct                                                                                                                                                                 <  1:670121/234‑1 (MQ=255)                                                                  atattcttcGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTAttt                                                                                                                                                            >  1:225663/4‑235 (MQ=255)                                                                  atattcttcGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGaa                                                                                                                                            >  1:727878/4‑251 (MQ=255)                                                                              agcGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAAcc                                                                                                                                  >  1:385261/3‑249 (MQ=255)                                                                                cGTAATCAGCTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAAtc                                                                                                                                                                      >  1:539316/1‑211 (MQ=255)                                                                                      cagttataCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTAttt                                                                                                                                                            >  1:345273/5‑215 (MQ=255)                                                                                      cagttataCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAAcc                                                                                                                                  >  1:473794/5‑241 (MQ=255)                                                                                      cagttataCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCa                                                                                                                        >  1:349469/5‑251 (MQ=255)                                                                                      cagttataCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCa                                                                                                                        >  1:297535/5‑251 (MQ=255)                                                                                        gttataCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAGAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTAttt                                                                                                                                                            >  1:789046/3‑213 (MQ=255)                                                                                        gttataCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTAttt                                                                                                                                                            <  1:2374/211‑1 (MQ=255)                                                                                        gttataCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTAttt                                                                                                                                                            >  1:801464/3‑213 (MQ=255)                                                                                        gttataCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCaaa                                                                                                                      >  1:560506/3‑251 (MQ=255)                                                                                        gttataCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCaaa                                                                                                                      >  1:760385/3‑251 (MQ=255)                                                                                        gttataCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCaaa                                                                                                                      >  1:97614/3‑251 (MQ=255)                                                                                        gttataCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCaaa                                                                                                                      >  1:228923/3‑251 (MQ=255)                                                                                           ataCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGaa                                                                                                                   >  1:694374/1‑251 (MQ=255)                                                                                            taCAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGaaa                                                                                                                  >  1:786500/1‑251 (MQ=255)                                                                                                aTGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAAt                                                                                                               >  1:707405/1‑250 (MQ=255)                                                                                                 tGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGaaatgttaacgaatc                                                                                                                                                                                                             >  1:492385/1‑142 (MQ=255)                                                                                                  gATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAAtcc                                                                                                             >  1:154306/1‑248 (MQ=255)                                                                                                    taatgggaccgaaaaaCTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTAttt                                                                                                                                                            <  1:685021/186‑1 (MQ=255)                                                                                                     aatgggaccgaaaaaCTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTAttt                                                                                                                                                            >  1:116916/15‑200 (MQ=255)                                                                                                         ggaccgaaaaaCTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGt                                                                                                                                                                                  <  1:875960/164‑1 (MQ=255)                                                                                                             cgaaaaaCTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAgg                                                                                                 <  1:421216/245‑1 (MQ=255)                                                                                                             cgaaaaaCTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAgg                                                                                                 <  1:802435/245‑1 (MQ=255)                                                                                                               aaaaaCTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCGCTGTATCAGGt                                                                                                <  1:621494/246‑1 (MQ=255)                                                                                                                  aaCTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAATTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAAccc                                                                                                                                 <  1:433342/213‑1 (MQ=255)                                                                                                                   aCTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGtgaccc                                                                                           <  1:316086/251‑6 (MQ=255)                                                                                                                              cTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGt                                                                                                <  1:158633/235‑1 (MQ=255)                                                                                                                                gacttAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAAtct                                                                                                                                                                     >  1:809505/4‑164 (MQ=255)                                                                                                                                gacttAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTc                                                                                                                                                <  1:316959/182‑1 (MQ=255)                                                                                                                                gacttAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAatcagc                                                                                                                                       <  1:842431/191‑1 (MQ=255)                                                                                                                                 acttAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTAttt                                                                                                                                                            >  1:8040/3‑172 (MQ=255)                                                                                                                                 acttAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGc                                                                              >  1:452542/3‑250 (MQ=255)                                                                                                                                      aCTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAAtctatc                                                                                                                                                                  <  1:193652/161‑4 (MQ=255)                                                                                                                                                            aaCTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAaca                                                  >  1:877908/1‑251 (MQ=255)                                                                                                                                                            aaCTTATTTTGTTTGAAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGt                                                                                                                                                                                  <  1:473485/123‑1 (MQ=255)                                                                                                                                                             aCTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAacac                                                 <  1:644617/251‑1 (MQ=255)                                                                                                                                                              cTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGt                                                                                                <  1:248754/203‑1 (MQ=255)                                                                                                                                                                   tttgtttgGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAg                                           <  1:205728/251‑1 (MQ=255)                                                                                                                                                                      gtttgGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGaaa                                        >  1:273175/1‑251 (MQ=255)                                                                                                                                                                         tgGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAAcc                                     <  1:744314/251‑1 (MQ=255)                                                                                                                                                                           gagaTAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAg                                   >  1:55804/1‑251 (MQ=255)                                                                                                                                                                             gaTAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGAc                                                                                                                             >  1:732628/1‑159 (MQ=255)                                                                                                                                                                             gaTAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAAc                                                                                                                          <  1:684249/162‑1 (MQ=255)                                                                                                                                                                             gaTAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTAt                                                                                                     <  1:266892/183‑1 (MQ=255)                                                                                                                                                                             gaTAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGt                                  >  1:888826/1‑250 (MQ=255)                                                                                                                                                                             gaTAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGt                                  >  1:30384/1‑250 (MQ=255)                                                                                                                                                                             gaTAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGt                                  >  1:869934/1‑250 (MQ=255)                                                                                                                                                                             gaTAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGt                                  >  1:840927/1‑250 (MQ=255)                                                                                                                                                                             gaTAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGCCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGt                                  >  1:852696/1‑250 (MQ=255)                                                                                                                                                                                       gtccAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGTCATTTGGTATg                       >  1:135509/3‑251 (MQ=255)                                                                                                                                                                                        tccAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGTCATTTGGTATgt                      <  1:81485/250‑2 (MQ=255)                                                                                                                                                                                           aaaTAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAg                                           >  1:138188/1‑227 (MQ=255)                                                                                                                                                                                           aaaTAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGTCATTTGGTATGTTaa                   >  1:85235/1‑251 (MQ=255)                                                                                                                                                                                               aaaGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGTCATTTGGTATGTTAAGTTc               <  1:462529/251‑1 (MQ=255)                                                                                                                                                                                                      gtgtCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGTCATTTGGTATGTTAAGTTCCTCTTc         <  1:15138/250‑1 (MQ=255)                                                                                                                                                                                                      gtgtCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGTCATTTGGTATGTTAAGTTCCTCTTCa        >  1:53332/1‑251 (MQ=255)                                                                                                                                                                                                       tgtCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGTCATTTGGTATGTTAAGTTCCTCTTCa        >  1:489688/1‑250 (MQ=255)                                                                                                                                                                                                         tCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAAccc                                                                                                                                 >  1:609688/1‑127 (MQ=255)                                                                                                                                                                                                         tCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAgg                                                                                                 >  1:465057/1‑159 (MQ=255)                                                                                                                                                                                                         tCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGTCATTTGGTATGTTAAGt                 >  1:424911/1‑239 (MQ=255)                                                                                                                                                                                                            cTATTGAGCACTGGGAAGAGAAAAAAAATCCTGAAATGTTAACGAATCTAGGCACATGTCGGCCTTATCGTAAGTTGGATGATAATCTATCTTATTTGTCGCCACGCTCAGAATCAGCTAACCCTGACAACCAAAGAAAAATCCCACTGTATCAGGTGACCCTTTGCGTTGAAGCCGGTAGCTTTTTCAAAAATTTAATAACACGTAAAGAAAACCAGTCATTTGGTATGTTAAGTTCCTCTTCACTAGCa  >  1:89932/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                AACAGACTTAGTTATTGTTCGTACGACCCAAGGGAAACTGGACAATGCCTCATACTCACTGGTCAATGTTCTTCGTGAACGTAATCAACTATACAATGATAATGGGAGCAAACCACTAAAAAGTACTGATTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGCCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAGTCCTGAATCATTAACGACTCTAAGTACCTGCCAGCCTTATCGTAAGTTGGATAATAATCTCTCTTATTTGTCACCACGCTCAGAAGCAGCAAACCCTGACAACCAAAGAAAAATTCCACTGTATCAGGTAACACTTTGTGTTGAAGCAGGTAGCTTTTTTAAAAATTTAATAACACGCAAAGAAAACCAGTCATTTGGTATGCTAAGTTCCTCTTCACTGGCA  >  1/1487606‑1488059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑