Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,487,630	G→T	T54T (ACG→ACT)	unknown →	Flp pilus assembly surface protein TadF, ATP/GTP‑binding motif

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,487,630	0	G	T	100.0%	43.6 / NA	18	T54T (ACG→ACT)	unknown	Flp pilus assembly surface protein TadF, ATP/GTP‑binding motif
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/9);  total (9/9)

ATAAAAGGGGCGCAGTTAGTCTTGAGTTCCTTTTTATGCTTATACTTTTAGTATTTATTTTCGCTTTTTTAACAGACTTAGTTATTGTTCGTACGACCCAAGGGAAACTGGACAATGCCTCATACTCACTGGTCAATGTTCTTCGTGAACGTAATCAACTATACAATGATAATGGGAGCAAACCACTAAAAAGTACTGATTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGCCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAGTCCTGAATCATTAACGACTCTAAGTACC  >  1/1487536‑1487864                                                                                               |                                                                                                                                                                                                                                           ataaAAAGGGCGCAGTTACGCTTGAGCTTCTTTTTATGCTTATTCTTTTAGTATTTATTTTCGCCTTTTTAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGAc                                                                                <  1:884263/251‑1 (MQ=255)                   cgcTTGAGCTTCTTTTTATGCTTATTCTTTTAGTATTTATTTTCGCCTTTTTAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGgt                                                              >  1:779644/3‑251 (MQ=255)                             tcTTTTTATGCTTATTCTTTTAGTATTTATTTTCGCCTTTTTAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGAc                                                                                <  1:459000/222‑1 (MQ=255)                              cTTTTTATGCTTATTCTTTTAGTATTTATTTTCGCCTTTTTAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACTGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGa                                                   >  1:67448/1‑251 (MQ=255)                                ttttATGCTTATTCTTTTAGTATTTATTTTCGCCTTTTTAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGATAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGc                                                 <  1:442647/251‑1 (MQ=255)                                           ttcTTTTAGTATTTATTTTCGCCTTTTTAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAagag                                      >  1:779739/3‑251 (MQ=255)                                           ttcTTTTAGTATTTATTTTCGCCTTTTTAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAagag                                      >  1:574359/3‑251 (MQ=255)                                                   gTATTTATTTTCGCCTTTTTAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTAt                                                      <  1:147517/227‑1 (MQ=255)                                                                cctttttAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGAc                                                                                >  1:281579/3‑188 (MQ=255)                                                                       aaCAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGaaatgttaacgaatc          <  1:651093/251‑14 (MQ=255)                                                                           gacctAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATaa                                                                     <  1:892138/184‑1 (MQ=255)                                                                           gacctAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTg                                                    >  1:664346/5‑205 (MQ=255)                                                                               tAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGAc                                                                                <  1:589346/173‑1 (MQ=255)                                                                               tAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCATTATTGAGCACTGGGAAGAGAAAAAAAATCCTGaaatgttaacgaatctaggcaca  >  1:720347/1‑230 (MQ=255)                                                                                aGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGaaatgttaacgaa            >  1:524724/1‑229 (MQ=255)                                                                                 gTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGgaga                                                                                       <  1:158211/164‑1 (MQ=255)                                                                                 gTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGGCAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGaaaaaaaa                              >  1:431347/1‑220 (MQ=255)                                                                                           gTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGAc                                                                                <  1:360430/161‑1 (MQ=255)                                                                                               |                                                                                                                                                                                                                                           ATAAAAGGGGCGCAGTTAGTCTTGAGTTCCTTTTTATGCTTATACTTTTAGTATTTATTTTCGCTTTTTTAACAGACTTAGTTATTGTTCGTACGACCCAAGGGAAACTGGACAATGCCTCATACTCACTGGTCAATGTTCTTCGTGAACGTAATCAACTATACAATGATAATGGGAGCAAACCACTAAAAAGTACTGATTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGCCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAGTCCTGAATCATTAACGACTCTAAGTACC  >  1/1487536‑1487864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑