Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,487,613	T→C	L49L (TTA→CTA)	unknown →	Flp pilus assembly surface protein TadF, ATP/GTP‑binding motif

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,487,613	0	T	C	100.0%	23.7 / NA	10	L49L (TTA→CTA)	unknown	Flp pilus assembly surface protein TadF, ATP/GTP‑binding motif
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/5);  total (5/5)

ATAAAAGGGGCGCAGTTAGTCTTGAGTTCCTTTTTATGCTTATACTTTTAGTATTTATTTTCGCTTTTTTAACAGACTTAGTTATTGTTCGTACGACCCAAGGGAAACTGGACAATGCCTCATACTCACTGGTCAATGTTCTTCGTGAACGTAATCAACTATACAATGATAATGGGAGCAAACCACTAAAAAGTACTGATTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGCCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAGTCCTGAATCATTAACGACTC  >  1/1487536‑1487856                                                                              |                                                                                                                                                                                                                                                    ataaAAAGGGCGCAGTTACGCTTGAGCTTCTTTTTATGCTTATTCTTTTAGTATTTATTTTCGCCTTTTTAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGAc                                                                        <  1:884263/251‑1 (MQ=255)                   cgcTTGAGCTTCTTTTTATGCTTATTCTTTTAGTATTTATTTTCGCCTTTTTAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGgt                                                      >  1:779644/3‑251 (MQ=255)                             tcTTTTTATGCTTATTCTTTTAGTATTTATTTTCGCCTTTTTAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGAc                                                                        <  1:459000/222‑1 (MQ=255)                              cTTTTTATGCTTATTCTTTTAGTATTTATTTTCGCCTTTTTAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACTGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGa                                           >  1:67448/1‑251 (MQ=255)                                ttttATGCTTATTCTTTTAGTATTTATTTTCGCCTTTTTAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGATAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGc                                         <  1:442647/251‑1 (MQ=255)                                           ttcTTTTAGTATTTATTTTCGCCTTTTTAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAagag                              >  1:574359/3‑251 (MQ=255)                                           ttcTTTTAGTATTTATTTTCGCCTTTTTAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAagag                              >  1:779739/3‑251 (MQ=255)                                                   gTATTTATTTTCGCCTTTTTAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTAt                                              <  1:147517/227‑1 (MQ=255)                                                                cctttttAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGAc                                                                        >  1:281579/3‑188 (MQ=255)                                                                       aaCAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAATCCTGaaatgttaacgaatc  <  1:651093/251‑14 (MQ=255)                                                                              |                                                                                                                                                                                                                                                    ATAAAAGGGGCGCAGTTAGTCTTGAGTTCCTTTTTATGCTTATACTTTTAGTATTTATTTTCGCTTTTTTAACAGACTTAGTTATTGTTCGTACGACCCAAGGGAAACTGGACAATGCCTCATACTCACTGGTCAATGTTCTTCGTGAACGTAATCAACTATACAATGATAATGGGAGCAAACCACTAAAAAGTACTGATTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGCCCAAATAAAGTCGGTGTCACTATTGAGCACTGGGAAGAGAAAAAAAGTCCTGAATCATTAACGACTC  >  1/1487536‑1487856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑