Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,487,579	A→T	I37I (ATA→ATT)	unknown →	Flp pilus assembly surface protein TadF, ATP/GTP‑binding motif

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,487,579	0	A	T	100.0%	11.3 / NA	6	I37I (ATA→ATT)	unknown	Flp pilus assembly surface protein TadF, ATP/GTP‑binding motif
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (3/3);  total (3/3)

AGGATAGTGTTGGCGGTTTATTGTCACGTAAGTTTGTAGCCGTACAGGAATTTAATAGATCTAAATTCCAATATCCCGGCAGGTCAAATAAATAATCAGGCAATTTACAATTATTTAAAGCATAAATAATATAATCACAGATGGCTAATCACTGGTATAAAAACATGAAAAAAAACATTCTCACTAATACTAAAAAATTTTTTCATAATAAAAGGGGCGCAGTTAGTCTTGAGTTCCTTTTTATGCTTATACTTTTAGTATTTATTTTCGCTTTTTTAACAGACTTAGTTATTGTTCGTACGACCCAAGGGAAACTGGACAATGCCTCATACTCACTGGTCAATGTTCTTCGTGAACGTAATCAACTATACAATGATAATGGGAGCAAACCACTAAAAAGTACTGATTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGCCCAAATAAAGTCGGTGTCACTATTGAGC  >  1/1487329‑1487817                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               gggataacgttagcagTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAATTTCAGCATCCAGGTAGGTCAAACAAATAAT‑‑‑‑TAATTCATGATTATTTAAAGCACGAGTAATATAATTGCAAATGGTTAATCACCGGTATAAAAACATGAAAAAAAATATCATCACTAGTATTAAAAAATTTTTTCGTAATAAAAAGGGCGCAGTTACGCTTGAGCTTCTTTTTATGCTTATTCttt                                                                                                                                                                                                                                            >  1:335287/16‑251 (MQ=255)                                                                                                                                                                                                                ataaAAAGGGCGCAGTTACGCTTGAGCTTCTTTTTATGCTTATTCTTTTAGTATTTATTTTCGCCTTTTTAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGAc                                 <  1:884263/251‑1 (MQ=255)                                                                                                                                                                                                                                  cgcTTGAGCTTCTTTTTATGCTTATTCTTTTAGTATTTATTTTCGCCTTTTTAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGgt               >  1:779644/3‑251 (MQ=255)                                                                                                                                                                                                                                            tcTTTTTATGCTTATTCTTTTAGTATTTATTTTCGCCTTTTTAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGAc                                 <  1:459000/222‑1 (MQ=255)                                                                                                                                                                                                                                             cTTTTTATGCTTATTCTTTTAGTATTTATTTTCGCCTTTTTAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGACAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACTGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGa    >  1:67448/1‑251 (MQ=255)                                                                                                                                                                                                                                               ttttATGCTTATTCTTTTAGTATTTATTTTCGCCTTTTTAACAGACCTAGTTATTGTTCGTACTACCCAGGGAAAACTGGATAATGCCTCGTATTCACTGGTCAATATTCTTCGCGAGCGTAATCAGTTATACAATGATAATGGGACCGAAAAACTAAAAAGTACTGACTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGTCCAAATAAAGTCGGTGTCACTATTGAGc  <  1:442647/251‑1 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               AGGATAGTGTTGGCGGTTTATTGTCACGTAAGTTTGTAGCCGTACAGGAATTTAATAGATCTAAATTCCAATATCCCGGCAGGTCAAATAAATAATCAGGCAATTTACAATTATTTAAAGCATAAATAATATAATCACAGATGGCTAATCACTGGTATAAAAACATGAAAAAAAACATTCTCACTAATACTAAAAAATTTTTTCATAATAAAAGGGGCGCAGTTAGTCTTGAGTTCCTTTTTATGCTTATACTTTTAGTATTTATTTTCGCTTTTTTAACAGACTTAGTTATTGTTCGTACGACCCAAGGGAAACTGGACAATGCCTCATACTCACTGGTCAATGTTCTTCGTGAACGTAATCAACTATACAATGATAATGGGAGCAAACCACTAAAAAGTACTGATTTAACTGAATATGAAAAAATGGCAAAACTTATTTTGTTTGGAGATAAAGACAGCCCAAATAAAGTCGGTGTCACTATTGAGC  >  1/1487329‑1487817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑