Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,487,375	G→A	Q176Q (CAG→CAA)	unknown →	Flp pilus assembly membrane protein TadE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,487,375	0	G	A	100.0%	25.1 / NA	11	Q176Q (CAG→CAA)	unknown	Flp pilus assembly membrane protein TadE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/8);  total (3/8)

CGGTTACAAGATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGTTCTTGTGTTAAGTCTTTATTGGATGGTAGGTTTAGACGGCCTACTAAAGATAGAGACGGTAAGATTATACCCCCCAATGGTCGGTTAGCAACATTGGCTGTATATTCATTGACCTACAATTATGAGTTTCTGGTTTCACTGCCATTTTTACCTAAGGATAGTGTTGGCGGTTTATTGTCACGTAAGTTTGTAGCCGTACAGGAATTTAATAGATCTAAATTCCAATATCCCGGCAGGTCAAATAAATAATCAGGCAATTTACAATTATTTAAAGCATAAATAATATAATCACAGATGGCTAATCACTGGTATAAAAACATGAAAAAAAACATTCTCACTAATACTAAAAAATTTTTTCATAATAAAAGGGGCGCAGTTAGTCTTGAGTTCCTTTTTATGCTTATACTTT  >  1/1487129‑1487583                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                 cggttatgaaaTTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGaa                                                                                                                                                                                                               <  1:164474/240‑1 (MQ=255)      atgaaaTTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCTTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAAt                                                                                                                                                                                                         <  1:424603/246‑1 (MQ=255)                                tgtgGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAAtttcagcatccaggtag                                                                                                                                                                               <  1:462618/251‑16 (MQ=255)                                 gtgGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAAtttcagcatccaggtagg                                                                                                                                                                              >  1:355380/1‑235 (MQ=255)                                 gtgGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAAtttcagcatccaggtagg                                                                                                                                                                              >  1:38067/1‑235 (MQ=255)                                                                            gTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAATTTCAGCATCCAGGTAGGTCAAACAaataattaattcatga                                                                                                                                                       <  1:768933/231‑11 (MQ=255)                                                                                     gCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAAt                                                                                                                                                                                               <  1:129261/182‑1 (MQ=255)                                                                                         acaaaaGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAATTTCAGCATCCAGGTAGGTCaaaca                                                                                                                                                                       <  1:502462/199‑3 (MQ=255)                                                                                                                                                       ttCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAATTTCAGCATCCAGGTAGGTCAaacaaata                                                                                                                                                                   <  1:78544/144‑1 (MQ=255)                                                                                                                                                                                    tttCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAATTTCAGCATCCAGGTAGGTCAAACAAATAAT‑‑‑‑TAATTCATGATTATTTAAAGCACGAGTAATATAATTGCAAATGGTTAATCACCGGTATAAAAACATGAAAAAAAATATCATCACTAGTATTAAAAAATTTTTTCGTAATAAAAAGGGCGCAGTTACGCTTGAgc                       <  1:267921/251‑2 (MQ=255)                                                                                                                                                                                                         gggataacgttagcagTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAATTTCAGCATCCAGGTAGGTCAAACAAATAAT‑‑‑‑TAATTCATGATTATTTAAAGCACGAGTAATATAATTGCAAATGGTTAATCACCGGTATAAAAACATGAAAAAAAATATCATCACTAGTATTAAAAAATTTTTTCGTAATAAAAAGGGCGCAGTTACGCTTGAGCTTCTTTTTATGCTTATTCttt  >  1:335287/16‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                 CGGTTACAAGATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGTTCTTGTGTTAAGTCTTTATTGGATGGTAGGTTTAGACGGCCTACTAAAGATAGAGACGGTAAGATTATACCCCCCAATGGTCGGTTAGCAACATTGGCTGTATATTCATTGACCTACAATTATGAGTTTCTGGTTTCACTGCCATTTTTACCTAAGGATAGTGTTGGCGGTTTATTGTCACGTAAGTTTGTAGCCGTACAGGAATTTAATAGATCTAAATTCCAATATCCCGGCAGGTCAAATAAATAATCAGGCAATTTACAATTATTTAAAGCATAAATAATATAATCACAGATGGCTAATCACTGGTATAAAAACATGAAAAAAAACATTCTCACTAATACTAAAAAATTTTTTCATAATAAAAGGGGCGCAGTTAGTCTTGAGTTCCTTTTTATGCTTATACTTT  >  1/1487129‑1487583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑