Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,487,329	A→G	K161R (AAG→AGG)	unknown →	Flp pilus assembly membrane protein TadE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,487,329	0	A	G	100.0%	41.9 / NA	17	K161R (AAG→AGG)	unknown	Flp pilus assembly membrane protein TadE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/11);  total (6/11)

CTTCAACTTTAGGTTATCTCGCCCGTTTGGATAAAAATGGCGGTTACAAGATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGTTCTTGTGTTAAGTCTTTATTGGATGGTAGGTTTAGACGGCCTACTAAAGATAGAGACGGTAAGATTATACCCCCCAATGGTCGGTTAGCAACATTGGCTGTATATTCATTGACCTACAATTATGAGTTTCTGGTTTCACTGCCATTTTTACCTAAGGATAGTGTTGGCGGTTTATTGTCACGTAAGTTTGTAGCCGTACAGGAATTTAATAGATCTAAATTCCAATATCCCGGCAGGTCAAATAAATAATCAGGCAATTTACAATTATTTAAAGCATAAATAATATAATCACAGATGGCTAATCACTGGTATAAAAACATGAAAAAAAACATTCTCACTAATACTAAAAAATTTTTTCATAATAAAAGGGGCGCAGTTAGTCTTGAGT  >  1/1487089‑1487562                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                          cctCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAAATGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGGTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGGATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATaacgtt                                                                                                                                                                                                                                 >  1:162447/3‑246 (MQ=255)         atAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATaacgttagcag                                                                                                                                                                                                                            <  1:483397/247‑11 (MQ=255)                aTCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATTTTTATTAGATGGGAAGGTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATAACCCCCAATGGTAGGTTAGCAAAATTGGCTGGGTATTCATTAACCTACAAGTATGAAATTTTGGTTTCGCTGCCATTTTTACCTAGGGATaacgtttgcaggttgttgtta                                                                                                                                                                                                                  >  1:649348/1‑231 (MQ=255)                                   aaaTGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGt                                                                                                                                                                                                           <  1:392231/239‑1 (MQ=255)                                         cggttatgaaaTTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGaa                                                                                                                                                                                          <  1:164474/240‑1 (MQ=255)                                              atgaaaTTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCTTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAAt                                                                                                                                                                                    <  1:424603/246‑1 (MQ=255)                                              atgaaaTTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATaacg                                                                                                                                                                                                                                   >  1:875529/6‑201 (MQ=255)                                                         gTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATaacgttagc                                                                                                                                                                                                                              <  1:446746/198‑9 (MQ=255)                                                           tAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATaacg                                                                                                                                                                                                                                   >  1:839709/1‑188 (MQ=255)                                                                        tgtgGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAAtttcagcatccaggtag                                                                                                                                                          <  1:462618/251‑16 (MQ=255)                                                                         gtgGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAAtttcagcatccaggtagg                                                                                                                                                         >  1:38067/1‑235 (MQ=255)                                                                         gtgGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAAtttcagcatccaggtagg                                                                                                                                                         >  1:355380/1‑235 (MQ=255)                                                                                                                    gTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAATTTCAGCATCCAGGTAGGTCAAACAaataattaattcatga                                                                                                                                  <  1:768933/231‑11 (MQ=255)                                                                                                                             gCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAAt                                                                                                                                                                          <  1:129261/182‑1 (MQ=255)                                                                                                                                 acaaaaGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAATTTCAGCATCCAGGTAGGTCaaaca                                                                                                                                                  <  1:502462/199‑3 (MQ=255)                                                                                                                                                                                               ttCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAATTTCAGCATCCAGGTAGGTCAaacaaata                                                                                                                                              <  1:78544/144‑1 (MQ=255)                                                                                                                                                                                                                            tttCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAATTTCAGCATCCAGGTAGGTCAAACAAATAAT‑‑‑‑TAATTCATGATTATTTAAAGCACGAGTAATATAATTGCAAATGGTTAATCACCGGTATAAAAACATGAAAAAAAATATCATCACTAGTATTAAAAAATTTTTTCGTAATAAAAAGGGCGCAGTTACGCTTGAgc  <  1:267921/251‑2 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                          CTTCAACTTTAGGTTATCTCGCCCGTTTGGATAAAAATGGCGGTTACAAGATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGTTCTTGTGTTAAGTCTTTATTGGATGGTAGGTTTAGACGGCCTACTAAAGATAGAGACGGTAAGATTATACCCCCCAATGGTCGGTTAGCAACATTGGCTGTATATTCATTGACCTACAATTATGAGTTTCTGGTTTCACTGCCATTTTTACCTAAGGATAGTGTTGGCGGTTTATTGTCACGTAAGTTTGTAGCCGTACAGGAATTTAATAGATCTAAATTCCAATATCCCGGCAGGTCAAATAAATAATCAGGCAATTTACAATTATTTAAAGCATAAATAATATAATCACAGATGGCTAATCACTGGTATAAAAACATGAAAAAAAACATTCTCACTAATACTAAAAAATTTTTTCATAATAAAAGGGGCGCAGTTAGTCTTGAGT  >  1/1487089‑1487562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑