Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,487,256	C→A	R137R (CGG→AGG)	unknown →	Flp pilus assembly membrane protein TadE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,487,256	0	C	A	100.0%	83.8 / NA	32	R137R (CGG→AGG)	unknown	Flp pilus assembly membrane protein TadE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (7/25);  total (7/25)

TAAAAATAGAACTGCAGAGGGTAATGATTATGCGGTAGAATTTGAGAAAGCTCTAAAACAACAGCTTGTTTATCAAGAATCTTCAACTTTAGGTTATCTCGCCCGTTTGGATAAAAATGGCGGTTACAAGATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGTTCTTGTGTTAAGTCTTTATTGGATGGTAGGTTTAGACGGCCTACTAAAGATAGAGACGGTAAGATTATACCCCCC‑AATGGTCGGTTAGCAACATTGGCTGTATATTCATTGACCTACAATTATGAGTTTCTGGTTTCACTGCCATTTTTACCTAAGGATAGTGTTGGCGGTTTATTGTCACGTAAGTTTGTAGCCGTACAGGAATTTAATAGATCTAAATTCCAATATCCCGGCAGGTCAAATAAATAATCAGGCAATTT  >  1/1487009‑1487434                                                                                                                                                                                                                                                         |                                                                                                                                                                                   tAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGt                                                                                                                                                                                 <  1:151568/251‑1 (MQ=255)          aacggcggaaggTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAAc                                                                                                                                                                          <  1:323401/239‑1 (MQ=255)                 gaaggTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTg                                                                                                                                                                  <  1:110985/247‑1 (MQ=255)                 gaaggTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTg                                                                                                                                                                  <  1:640058/247‑1 (MQ=255)                         cgATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCAt                                                                                                                                                         <  1:399960/250‑1 (MQ=255)                         cgATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCAt                                                                                                                                                         <  1:480908/250‑1 (MQ=255)                           aTTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCTCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCAtt                                                                                                                                                        <  1:634449/250‑1 (MQ=255)                           aTTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCAtt                                                                                                                                                        <  1:784424/250‑1 (MQ=255)                             tatgctgcaGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCAttaac                                                                                                                                                     <  1:841116/243‑4 (MQ=255)                                      gAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCa                                                                                                                                                          <  1:247496/237‑1 (MQ=255)                                        aTTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCt                                                                                                                                                   <  1:724858/242‑1 (MQ=255)                                                                acttatttATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCAttt                                                                                                                   <  1:160468/246‑1 (MQ=255)                                                                         tCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATTAAATTGATGTTTAATATGTTGATTGTGGGAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGGAGGTTAGCAACATTGGCTGTGTATTTATTAACCTACAAGTATTAATTTTTTGTTTTGCTGCCATTTTTACCTaggg                                                                                                         >  1:492464/1‑248 (MQ=255)                                                                          cAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCa                                                                                                                                                          <  1:871881/201‑1 (MQ=255)                                                                                acctCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCt                                                                                                                                                   <  1:854501/199‑1 (MQ=255)                                                                                 cctCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAAATGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGGTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGGATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATaacgtt                                                                                                 >  1:162447/3‑246 (MQ=255)                                                                                      actatAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTAc                                                                                                               <  1:163409/228‑1 (MQ=255)                                                                                         atAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATaacgttagcag                                                                                            <  1:483397/247‑11 (MQ=255)                                                                                                aTCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATTTTTATTAGATGGGAAGGTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATAACCCCC‑AATGGTAGGTTAGCAAAATTGGCTGGGTATTCATTAACCTACAAGTATGAAATTTTGGTTTCGCTGCCATTTTTACCTAGGGATaacgtttgcaggttgttgtta                                                                                  >  1:649348/1‑231 (MQ=255)                                                                                                                   aaaTGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGt                                                                           <  1:392231/239‑1 (MQ=255)                                                                                                                         cggttatgaaaTTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGaa                                                          <  1:164474/240‑1 (MQ=255)                                                                                                                            ttatgaaaTTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAAc                                                                                                                                                                          <  1:872591/128‑1 (MQ=255)                                                                                                                              atgaaaTTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCTTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAAt                                                    <  1:424603/246‑1 (MQ=255)                                                                                                                              atgaaaTTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATaacg                                                                                                   >  1:875529/6‑201 (MQ=255)                                                                                                                                         gTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATaacgttagc                                                                                              <  1:446746/198‑9 (MQ=255)                                                                                                                                           tAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATaacg                                                                                                   >  1:839709/1‑188 (MQ=255)                                                                                                                                                        tgtgGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAAtttcagcatccaggtag                          <  1:462618/251‑16 (MQ=255)                                                                                                                                                         gtgGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAAtttcagcatccaggtagg                         >  1:38067/1‑235 (MQ=255)                                                                                                                                                         gtgGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAAtttcagcatccaggtagg                         >  1:355380/1‑235 (MQ=255)                                                                                                                                                                                                    gTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAATTTCAGCATCCAGGTAGGTCAAACAaataattaattcatga  <  1:768933/231‑11 (MQ=255)                                                                                                                                                                                                             gCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAAt                                          <  1:129261/182‑1 (MQ=255)                                                                                                                                                                                                                 acaaaaGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAATTTCAGCATCCAGGTAGGTCaaaca                  <  1:502462/199‑3 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                   TAAAAATAGAACTGCAGAGGGTAATGATTATGCGGTAGAATTTGAGAAAGCTCTAAAACAACAGCTTGTTTATCAAGAATCTTCAACTTTAGGTTATCTCGCCCGTTTGGATAAAAATGGCGGTTACAAGATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGTTCTTGTGTTAAGTCTTTATTGGATGGTAGGTTTAGACGGCCTACTAAAGATAGAGACGGTAAGATTATACCCCCC‑AATGGTCGGTTAGCAACATTGGCTGTATATTCATTGACCTACAATTATGAGTTTCTGGTTTCACTGCCATTTTTACCTAAGGATAGTGTTGGCGGTTTATTGTCACGTAAGTTTGTAGCCGTACAGGAATTTAATAGATCTAAATTCCAATATCCCGGCAGGTCAAATAAATAATCAGGCAATTT  >  1/1487009‑1487434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑