Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,487,212	G→A	R122Q (CGG→CAG)	unknown →	Flp pilus assembly membrane protein TadE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,487,212	0	G	A	100.0%	125.6 / NA	48	R122Q (CGG→CAG)	unknown	Flp pilus assembly membrane protein TadE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/38);  total (10/38)

TACTGGGACTTGGCTATCACTGAAAGTGCAAGAATTGCTAAAAATAGAACTGCAGAGGGTAATGATTATGCGGTAGAATTTGAGAAAGCTCTAAAACAACAGCTTGTTTATCAAGAATCTTCAACTTTAGGTTATCTCGCCCGTTTGGATAAAAATGGCGGTTACAAGATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGTTCTTGTGTTAAGTCTTTATTGGATGGTAGGTTTAGACGGCCTACTAAAGATAGAGACGGTAAGATTATACCCCCC‑AATGGTCGGTTAGCAACATTGGCTGTATATTCATTGACCTACAATTATGAGTTTCTGGTTTCACTGCCATTTTTACCTAAGGATAGTGTTGGCGGTTTATTGTCACGTAAGTTTGTAGCCGTACAGGAATTTAATAGATCTAAATTCCAATATCCCGGCAGGTCAAATAAATAATCAGGCAATTT  >  1/1486971‑1487434                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                tattgggatttagccataaCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCctacaaa                                                                                                                                                                                                                        <  1:303148/233‑4 (MQ=255)          ttagccataaCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACagaaa                                                                                                                                                                                                               <  1:602452/242‑3 (MQ=255)                  aaCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTTTTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTaata                                                                                                                                                                                                       >  1:560789/2‑249 (MQ=255)                   aCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTaatat                                                                                                                                                                                                      <  1:8590/251‑4 (MQ=255)                        aaGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATAc                                                                                                                                                                                                 <  1:482367/251‑1 (MQ=255)                        aaGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATAc                                                                                                                                                                                                 <  1:332321/251‑1 (MQ=255)                        aaGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATAc                                                                                                                                                                                                 <  1:606470/251‑1 (MQ=255)                        aaGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATAc                                                                                                                                                                                                 <  1:303701/251‑1 (MQ=255)                          gTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTTATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTTTTAGATGGTAAGTTTAGGCAGCCTACAAAAGAAAGAAACGGTAATATTATAc                                                                                                                                                                                                 >  1:485284/1‑249 (MQ=255)                          gTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTaatat                                                                                                                                                                                                      <  1:120730/244‑4 (MQ=255)                                  atcgCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATgg                                                                                                                                                                                      <  1:237468/248‑1 (MQ=255)                                   tcgCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGt                                                                                                                                                                                     <  1:223428/249‑1 (MQ=255)                                       tAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGt                                                                                                                                                                                 <  1:151568/251‑1 (MQ=255)                                                aacggcggaaggTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAAc                                                                                                                                                                          <  1:323401/239‑1 (MQ=255)                                                       gaaggTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTg                                                                                                                                                                  <  1:110985/247‑1 (MQ=255)                                                       gaaggTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTg                                                                                                                                                                  <  1:640058/247‑1 (MQ=255)                                                               cgATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCAt                                                                                                                                                         <  1:480908/250‑1 (MQ=255)                                                               cgATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCAt                                                                                                                                                         <  1:399960/250‑1 (MQ=255)                                                                 aTTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCTCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCAtt                                                                                                                                                        <  1:634449/250‑1 (MQ=255)                                                                 aTTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCAtt                                                                                                                                                        <  1:784424/250‑1 (MQ=255)                                                                   tatgctgcaGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCAttaac                                                                                                                                                     <  1:841116/243‑4 (MQ=255)                                                                            gAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTaatat                                                                                                                                                                                                      <  1:168314/194‑4 (MQ=255)                                                                            gAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCa                                                                                                                                                          <  1:247496/237‑1 (MQ=255)                                                                              aTTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCt                                                                                                                                                   <  1:724858/242‑1 (MQ=255)                                                                                           ctAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATgg                                                                                                                                                                                      <  1:507792/194‑1 (MQ=255)                                                                                                      acttatttATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAAATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCAttt                                                                                                                   <  1:160468/246‑1 (MQ=255)                                                                                                               tCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATTAAATTGATGTTTAATATGTTGATTGTGGGAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGGAGGTTAGCAACATTGGCTGTGTATTTATTAACCTACAAGTATTAATTTTTTGTTTTGCTGCCATTTTTACCTaggg                                                                                                         >  1:492464/1‑248 (MQ=255)                                                                                                                cAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCa                                                                                                                                                          <  1:871881/201‑1 (MQ=255)                                                                                                                      acctCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCt                                                                                                                                                   <  1:854501/199‑1 (MQ=255)                                                                                                                       cctCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAAATGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGGTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGGATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATaacgtt                                                                                                 >  1:162447/3‑246 (MQ=255)                                                                                                                            actatAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AAt                                                                                                                                                                                        <  1:856712/155‑1 (MQ=255)                                                                                                                            actatAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGtagg                                                                                                                                                                                  <  1:450764/161‑4 (MQ=255)                                                                                                                            actatAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTAc                                                                                                               <  1:163409/228‑1 (MQ=255)                                                                                                                               atAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATaacgttagcag                                                                                            <  1:483397/247‑11 (MQ=255)                                                                                                                                      aTCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATTTTTATTAGATGGGAAGGTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATAACCCCC‑AATGGTAGGTTAGCAAAATTGGCTGGGTATTCATTAACCTACAAGTATGAAATTTTGGTTTCGCTGCCATTTTTACCTAGGGATaacgtttgcaggttgttgtta                                                                                  >  1:649348/1‑231 (MQ=255)                                                                                                                                                         aaaTGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGt                                                                           <  1:392231/239‑1 (MQ=255)                                                                                                                                                               cggttatgaaaTTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGaa                                                          <  1:164474/240‑1 (MQ=255)                                                                                                                                                                  ttatgaaaTTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAAc                                                                                                                                                                          <  1:872591/128‑1 (MQ=255)                                                                                                                                                                    atgaaaTTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCTTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAAt                                                    <  1:424603/246‑1 (MQ=255)                                                                                                                                                                    atgaaaTTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATaacg                                                                                                   >  1:875529/6‑201 (MQ=255)                                                                                                                                                                            gATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATta                                                                                                                                                                                                    <  1:425287/100‑1 (MQ=255)                                                                                                                                                                               gTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAg                                                                                                                                                                                                                      >  1:206945/1‑79 (MQ=255)                                                                                                                                                                               gTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATaacgttagc                                                                                              <  1:446746/198‑9 (MQ=255)                                                                                                                                                                                 tAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATaacg                                                                                                   >  1:839709/1‑188 (MQ=255)                                                                                                                                                                                              tgtgGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAAtttcagcatccaggtag                          <  1:462618/251‑16 (MQ=255)                                                                                                                                                                                               gtgGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAAtttcagcatccaggtagg                         >  1:38067/1‑235 (MQ=255)                                                                                                                                                                                               gtgGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAAtttcagcatccaggtagg                         >  1:355380/1‑235 (MQ=255)                                                                                                                                                                                                                                          gTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCC‑AATGGTAGGTTAGCAACATTGGCTGTGTATTCATTAACCTACAAGTATGAATTTTTGGTTTCGCTGCCATTTTTACCTAGGGATAACGTTAGCAGTTTGTTGTCACGTAAGTTTGTAGCCGTACAAGAATTTAATAGATCTAAATTTCAGCATCCAGGTAGGTCAAACAaataattaattcatga  <  1:768933/231‑11 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                TACTGGGACTTGGCTATCACTGAAAGTGCAAGAATTGCTAAAAATAGAACTGCAGAGGGTAATGATTATGCGGTAGAATTTGAGAAAGCTCTAAAACAACAGCTTGTTTATCAAGAATCTTCAACTTTAGGTTATCTCGCCCGTTTGGATAAAAATGGCGGTTACAAGATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGTTCTTGTGTTAAGTCTTTATTGGATGGTAGGTTTAGACGGCCTACTAAAGATAGAGACGGTAAGATTATACCCCCC‑AATGGTCGGTTAGCAACATTGGCTGTATATTCATTGACCTACAATTATGAGTTTCTGGTTTCACTGCCATTTTTACCTAAGGATAGTGTTGGCGGTTTATTGTCACGTAAGTTTGTAGCCGTACAGGAATTTAATAGATCTAAATTCCAATATCCCGGCAGGTCAAATAAATAATCAGGCAATTT  >  1/1486971‑1487434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑