Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,487,042	G→T	A65A (GCG→GCT)	unknown →	Flp pilus assembly membrane protein TadE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,487,042	0	G	T	100.0%	125.1 / NA	47	A65A (GCG→GCT)	unknown	Flp pilus assembly membrane protein TadE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/34);  total (13/34)

AACGCGTATCTAATAATATCGCCAGATAAAAAAAGGTTCTAT‑AATGAAAAAATTCCTATCGAATAAGAAGGGAGCATCAACCGTTGAGTTTGCCTTGACGGTTGTATTTTATTTCTTTGTCGTATTCCTAATTCTAGAATTCTGTCGTATATCTATTACAACTGCGTACTGGGACTTGGCTATCACTGAAAGTGCAAGAATTGCTAAAAATAGAACTGCAGAGGGTAATGATTATGCGGTAGAATTTGAGAAAGCTCTAAAACAACAGCTTGTTTATCAAGAATCTTCAACTTTAGGTTATCTCGCCCGTTTGGATAAAAATGGCGGTTACAAGATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGTTCTTGTGTTAAGTCTTTATTGGATGGTAGGTTTAGACGGCCTACTAAAGATAGAGACGGTAAGATTATACCCCCCAATGGTCGGTTAGCAACATTGGCTGTATATTCATT  >  1/1486805‑1487284                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   aaCGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTgaaaa                                                                                                                                                                                                                                      <  1:423956/251‑4 (MQ=255)             gtaataTCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAaaca                                                                                                                                                                                                                          >  1:179337/2‑251 (MQ=255)                           gataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTgaaaa                                                                                                                                                                                                                                      >  1:570457/4‑224 (MQ=255)                           gataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTa                                                                                                                                                                                                              >  1:596994/4‑251 (MQ=255)                            ataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTgaaaa                                                                                                                                                                                                                                      >  1:663824/3‑223 (MQ=255)                            ataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACttatttat                                                                                                                                                                                                             >  1:138808/3‑251 (MQ=255)                            ataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACttatttat                                                                                                                                                                                                             >  1:304702/3‑251 (MQ=255)                                                 aaaaaTTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAggc                                                                                                                                                                                        <  1:335046/251‑2 (MQ=255)                                                               aaTAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACtat                                                                                                                                                                                            <  1:371139/233‑3 (MQ=255)                                                               aaTAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGttt                                                                                                                                                                          >  1:111173/1‑251 (MQ=255)                                                                                          tttGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTatgaaattg                                                                                                                                                <  1:274106/250‑9 (MQ=255)                                                                                                                      ttatCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTgag                                                                                                                   <  1:726941/248‑1 (MQ=255)                                                                                                                      ttatCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTgag                                                                                                                   <  1:690141/248‑1 (MQ=255)                                                                                                                        atCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGt                                                                                                                         <  1:292311/242‑1 (MQ=255)                                                                                                                              ttcttAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTtg                                                                                                           <  1:620439/247‑1 (MQ=255)                                                                                                                                       cTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTaaatc                                                                                                  <  1:360761/251‑4 (MQ=255)                                                                                                                                                        atCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTAtt                                                                                            <  1:768307/240‑1 (MQ=255)                                                                                                                                                        atCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTaagt                                                                                 >  1:696224/1‑248 (MQ=255)                                                                                                                                                         tCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTaaatc                                                                                                  <  1:140087/233‑4 (MQ=255)                                                                                                                                                          cTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAAATGATGTTAAATATGTTGATtg                                                                                                                             >  1:764995/1‑205 (MQ=255)                                                                                                                                                           tATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTa                                                                              >  1:731563/1‑251 (MQ=255)                                                                                                                                                                       atattgggatttagccataaCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTgag                                                                                                                   <  1:233803/183‑1 (MQ=255)                                                                                                                                                                        tattgggatttagccataaCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCctacaaa                                                                 <  1:303148/233‑4 (MQ=255)                                                                                                                                                                                 ttagccataaCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACagaaa                                                        <  1:602452/242‑3 (MQ=255)                                                                                                                                                                                         aaCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTaaatc                                                                                                  <  1:75084/200‑4 (MQ=255)                                                                                                                                                                                         aaCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTTTTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTaata                                                >  1:560789/2‑249 (MQ=255)                                                                                                                                                                                          aCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTaatat                                               <  1:8590/251‑4 (MQ=255)                                                                                                                                                                                               aaGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATAc                                          <  1:303701/251‑1 (MQ=255)                                                                                                                                                                                               aaGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATAc                                          <  1:482367/251‑1 (MQ=255)                                                                                                                                                                                               aaGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATAc                                          <  1:332321/251‑1 (MQ=255)                                                                                                                                                                                               aaGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATAc                                          <  1:606470/251‑1 (MQ=255)                                                                                                                                                                                                 gTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTTATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTTTTAGATGGTAAGTTTAGGCAGCCTACAAAAGAAAGAAACGGTAATATTATAc                                          >  1:485284/1‑249 (MQ=255)                                                                                                                                                                                                 gTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTaatat                                               <  1:120730/244‑4 (MQ=255)                                                                                                                                                                                                         atcgCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATgg                                <  1:237468/248‑1 (MQ=255)                                                                                                                                                                                                          tcgCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGt                               <  1:223428/249‑1 (MQ=255)                                                                                                                                                                                                            gCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTg                                                                                                                     <  1:684998/163‑1 (MQ=255)                                                                                                                                                                                                              tAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGt                           <  1:151568/251‑1 (MQ=255)                                                                                                                                                                                                                 aaacagaacggcggaaggTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTa                                                                                                      <  1:348243/157‑1 (MQ=255)                                                                                                                                                                                                                       aacggcggaaggTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAAc                    <  1:323401/239‑1 (MQ=255)                                                                                                                                                                                                                              gaaggTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTg            <  1:640058/247‑1 (MQ=255)                                                                                                                                                                                                                              gaaggTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTg            <  1:110985/247‑1 (MQ=255)                                                                                                                                                                                                                                 ggTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAggc                                                                           >  1:49506/1‑182 (MQ=255)                                                                                                                                                                                                                                      cgATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCAt   <  1:399960/250‑1 (MQ=255)                                                                                                                                                                                                                                      cgATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCAt   <  1:480908/250‑1 (MQ=255)                                                                                                                                                                                                                                        aTTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTaaatc                                                                                                  <  1:770977/154‑4 (MQ=255)                                                                                                                                                                                                                                        aTTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCTCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCAtt  <  1:634449/250‑1 (MQ=255)                                                                                                                                                                                                                                        aTTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGTTAGCAACATTGGCTGTGTATTCAtt  <  1:784424/250‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   AACGCGTATCTAATAATATCGCCAGATAAAAAAAGGTTCTAT‑AATGAAAAAATTCCTATCGAATAAGAAGGGAGCATCAACCGTTGAGTTTGCCTTGACGGTTGTATTTTATTTCTTTGTCGTATTCCTAATTCTAGAATTCTGTCGTATATCTATTACAACTGCGTACTGGGACTTGGCTATCACTGAAAGTGCAAGAATTGCTAAAAATAGAACTGCAGAGGGTAATGATTATGCGGTAGAATTTGAGAAAGCTCTAAAACAACAGCTTGTTTATCAAGAATCTTCAACTTTAGGTTATCTCGCCCGTTTGGATAAAAATGGCGGTTACAAGATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGTTCTTGTGTTAAGTCTTTATTGGATGGTAGGTTTAGACGGCCTACTAAAGATAGAGACGGTAAGATTATACCCCCCAATGGTCGGTTAGCAACATTGGCTGTATATTCATT  >  1/1486805‑1487284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑