Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,487,027	G→A	E60E (GAG→GAA)	unknown →	Flp pilus assembly membrane protein TadE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,487,027	0	G	A	100.0%	102.3 / NA	38	E60E (GAG→GAA)	unknown	Flp pilus assembly membrane protein TadE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/26);  total (12/26)

TAATGCTCTCAAAAAAACTGAACGCGTATCTAATAATATCGCCAGATAAAAAAAGGTTCTAT‑AATGAAAAAATTCCTATCGAATAAGAAGGGAGCATCAACCGTTGAGTTTGCCTTGACGGTTGTATTTTATTTCTTTGTCGTATTCCTAATTCTAGAATTCTGTCGTATATCTATTACAACTGCGTACTGGGACTTGGCTATCACTGAAAGTGCAAGAATTGCTAAAAATAGAACTGCAGAGGGTAATGATTATGCGGTAGAATTTGAGAAAGCTCTAAAACAACAGCTTGTTTATCAAGAATCTTCAACTTTAGGTTATCTCGCCCGTTTGGATAAAAATGGCGGTTACAAGATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGTTCTTGTGTTAAGTCTTTATTGGATGGTAGGTTTAGACGGCCTACTAAAGATAGAGACGGTAAGATTATACCCCCCAATGGTCGGT  >  1/1486785‑1487259                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                         taatGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGAt                                                                                                                                                                                                                                 <  1:482743/251‑1 (MQ=255)                     aaCGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTgaaaa                                                                                                                                                                                                             <  1:423956/251‑4 (MQ=255)                                 gtaataTCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAaaca                                                                                                                                                                                                 >  1:179337/2‑251 (MQ=255)                                               gataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTgaaaa                                                                                                                                                                                                             >  1:570457/4‑224 (MQ=255)                                               gataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTa                                                                                                                                                                                     >  1:596994/4‑251 (MQ=255)                                                ataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTgaaaa                                                                                                                                                                                                             >  1:663824/3‑223 (MQ=255)                                                ataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACttatttat                                                                                                                                                                                    >  1:138808/3‑251 (MQ=255)                                                ataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACttatttat                                                                                                                                                                                    >  1:304702/3‑251 (MQ=255)                                                                     aaaaaTTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAggc                                                                                                                                                               <  1:335046/251‑2 (MQ=255)                                                                                   aaTAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACtat                                                                                                                                                                   <  1:371139/233‑3 (MQ=255)                                                                                   aaTAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGttt                                                                                                                                                 >  1:111173/1‑251 (MQ=255)                                                                                                              tttGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTatgaaattg                                                                                                                       <  1:274106/250‑9 (MQ=255)                                                                                                                                          ttatCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTgag                                                                                          <  1:726941/248‑1 (MQ=255)                                                                                                                                          ttatCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTgag                                                                                          <  1:690141/248‑1 (MQ=255)                                                                                                                                            atCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGt                                                                                                <  1:292311/242‑1 (MQ=255)                                                                                                                                                  ttcttAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTtg                                                                                  <  1:620439/247‑1 (MQ=255)                                                                                                                                                           cTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTaaatc                                                                         <  1:360761/251‑4 (MQ=255)                                                                                                                                                                            atCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTAtt                                                                   <  1:768307/240‑1 (MQ=255)                                                                                                                                                                            atCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTaagt                                                        >  1:696224/1‑248 (MQ=255)                                                                                                                                                                             tCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTaaatc                                                                         <  1:140087/233‑4 (MQ=255)                                                                                                                                                                              cTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAAATGATGTTAAATATGTTGATtg                                                                                                    >  1:764995/1‑205 (MQ=255)                                                                                                                                                                               tATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTa                                                     >  1:731563/1‑251 (MQ=255)                                                                                                                                                                                           atattgggatttagccataaCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTgag                                                                                          <  1:233803/183‑1 (MQ=255)                                                                                                                                                                                            tattgggatttagccataaCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCctacaaa                                        <  1:303148/233‑4 (MQ=255)                                                                                                                                                                                                     ttagccataaCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACagaaa                               <  1:602452/242‑3 (MQ=255)                                                                                                                                                                                                             aaCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTaaatc                                                                         <  1:75084/200‑4 (MQ=255)                                                                                                                                                                                                             aaCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTTTTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTaata                       >  1:560789/2‑249 (MQ=255)                                                                                                                                                                                                              aCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTaatat                      <  1:8590/251‑4 (MQ=255)                                                                                                                                                                                                                   aaGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATAc                 <  1:482367/251‑1 (MQ=255)                                                                                                                                                                                                                   aaGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATAc                 <  1:606470/251‑1 (MQ=255)                                                                                                                                                                                                                   aaGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATAc                 <  1:332321/251‑1 (MQ=255)                                                                                                                                                                                                                   aaGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATAc                 <  1:303701/251‑1 (MQ=255)                                                                                                                                                                                                                     gTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTTATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTTTTAGATGGTAAGTTTAGGCAGCCTACAAAAGAAAGAAACGGTAATATTATAc                 >  1:485284/1‑249 (MQ=255)                                                                                                                                                                                                                     gTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTaatat                      <  1:120730/244‑4 (MQ=255)                                                                                                                                                                                                                             atcgCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATgg       <  1:237468/248‑1 (MQ=255)                                                                                                                                                                                                                              tcgCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGt      <  1:223428/249‑1 (MQ=255)                                                                                                                                                                                                                                gCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTg                                                                                            <  1:684998/163‑1 (MQ=255)                                                                                                                                                                                                                                  tAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATACCCCCCAATGGTAGGt  <  1:151568/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                         TAATGCTCTCAAAAAAACTGAACGCGTATCTAATAATATCGCCAGATAAAAAAAGGTTCTAT‑AATGAAAAAATTCCTATCGAATAAGAAGGGAGCATCAACCGTTGAGTTTGCCTTGACGGTTGTATTTTATTTCTTTGTCGTATTCCTAATTCTAGAATTCTGTCGTATATCTATTACAACTGCGTACTGGGACTTGGCTATCACTGAAAGTGCAAGAATTGCTAAAAATAGAACTGCAGAGGGTAATGATTATGCGGTAGAATTTGAGAAAGCTCTAAAACAACAGCTTGTTTATCAAGAATCTTCAACTTTAGGTTATCTCGCCCGTTTGGATAAAAATGGCGGTTACAAGATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGTTCTTGTGTTAAGTCTTTATTGGATGGTAGGTTTAGACGGCCTACTAAAGATAGAGACGGTAAGATTATACCCCCCAATGGTCGGT  >  1/1486785‑1487259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑