Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,487,006	T→C	I53I (ATT→ATC)	unknown →	Flp pilus assembly membrane protein TadE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,487,006	0	T	C	100.0%	95.1 / NA	36	I53I (ATT→ATC)	unknown	Flp pilus assembly membrane protein TadE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/23);  total (13/23)

TACATCTCCTGATGATTTAATTAATGCTCTCAAAAAAACTGAACGCGTATCTAATAATATCGCCAGATAAAAAAAGGTTCTAT‑AATGAAAAAATTCCTATCGAATAAGAAGGGAGCATCAACCGTTGAGTTTGCCTTGACGGTTGTATTTTATTTCTTTGTCGTATTCCTAATTCTAGAATTCTGTCGTATATCTATTACAACTGCGTACTGGGACTTGGCTATCACTGAAAGTGCAAGAATTGCTAAAAATAGAACTGCAGAGGGTAATGATTATGCGGTAGAATTTGAGAAAGCTCTAAAACAACAGCTTGTTTATCAAGAATCTTCAACTTTAGGTTATCTCGCCCGTTTGGATAAAAATGGCGGTTACAAGATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGTTCTTGTGTTAAGTCTTTATTGGATGGTAGGTTTAGACGGCCTACTAAAGATAGAGACGGTAAGATTATAC  >  1/1486764‑1487244                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               caCATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTaaaaac                                                                                                                                                                                                                                       <  1:494047/250‑2 (MQ=255)     tccccTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGaa                                                                                                                                                                                                                                   >  1:35572/4‑251 (MQ=255)                      taatGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGAt                                                                                                                                                                                                                  <  1:482743/251‑1 (MQ=255)                                          aaCGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTgaaaa                                                                                                                                                                                              <  1:423956/251‑4 (MQ=255)                                                      gtaataTCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAaaca                                                                                                                                                                                  >  1:179337/2‑251 (MQ=255)                                                                    gataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTgaaaa                                                                                                                                                                                              >  1:570457/4‑224 (MQ=255)                                                                    gataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTa                                                                                                                                                                      >  1:596994/4‑251 (MQ=255)                                                                     ataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTgaaaa                                                                                                                                                                                              >  1:663824/3‑223 (MQ=255)                                                                     ataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACttatttat                                                                                                                                                                     >  1:138808/3‑251 (MQ=255)                                                                     ataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACttatttat                                                                                                                                                                     >  1:304702/3‑251 (MQ=255)                                                                                          aaaaaTTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAggc                                                                                                                                                <  1:335046/251‑2 (MQ=255)                                                                                                        aaTAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACtat                                                                                                                                                    <  1:371139/233‑3 (MQ=255)                                                                                                        aaTAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGttt                                                                                                                                  >  1:111173/1‑251 (MQ=255)                                                                                                                                   tttGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTatgaaattg                                                                                                        <  1:274106/250‑9 (MQ=255)                                                                                                                                                               ttatCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTgag                                                                           <  1:690141/248‑1 (MQ=255)                                                                                                                                                               ttatCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTgag                                                                           <  1:726941/248‑1 (MQ=255)                                                                                                                                                                 atCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGt                                                                                 <  1:292311/242‑1 (MQ=255)                                                                                                                                                                       ttcttAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTtg                                                                   <  1:620439/247‑1 (MQ=255)                                                                                                                                                                                cTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTaaatc                                                          <  1:360761/251‑4 (MQ=255)                                                                                                                                                                                                 atCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTAtt                                                    <  1:768307/240‑1 (MQ=255)                                                                                                                                                                                                 atCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTaagt                                         >  1:696224/1‑248 (MQ=255)                                                                                                                                                                                                  tCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTaaatc                                                          <  1:140087/233‑4 (MQ=255)                                                                                                                                                                                                   cTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAAATGATGTTAAATATGTTGATtg                                                                                     >  1:764995/1‑205 (MQ=255)                                                                                                                                                                                                    tATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTa                                      >  1:731563/1‑251 (MQ=255)                                                                                                                                                                                                                atattgggatttagccataaCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTgag                                                                           <  1:233803/183‑1 (MQ=255)                                                                                                                                                                                                                 tattgggatttagccataaCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCctacaaa                         <  1:303148/233‑4 (MQ=255)                                                                                                                                                                                                                          ttagccataaCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACagaaa                <  1:602452/242‑3 (MQ=255)                                                                                                                                                                                                                                  aaCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTaaatc                                                          <  1:75084/200‑4 (MQ=255)                                                                                                                                                                                                                                  aaCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTTTTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTaata        >  1:560789/2‑249 (MQ=255)                                                                                                                                                                                                                                   aCTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTaatat       <  1:8590/251‑4 (MQ=255)                                                                                                                                                                                                                                        aaGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATAc  <  1:606470/251‑1 (MQ=255)                                                                                                                                                                                                                                        aaGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATAc  <  1:482367/251‑1 (MQ=255)                                                                                                                                                                                                                                        aaGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATAc  <  1:332321/251‑1 (MQ=255)                                                                                                                                                                                                                                        aaGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTAATATTATAc  <  1:303701/251‑1 (MQ=255)                                                                                                                                                                                                                                          gTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTTATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTTTTAGATGGTAAGTTTAGGCAGCCTACAAAAGAAAGAAACGGTAATATTATAc  >  1:485284/1‑249 (MQ=255)                                                                                                                                                                                                                                          gTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTATGAAATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGCTCTTGTATTAAATCTTTATTAGATGGTAAGTTTAGGCAGCCTACAAAAGACAGAAACGGTaatat       <  1:120730/244‑4 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               TACATCTCCTGATGATTTAATTAATGCTCTCAAAAAAACTGAACGCGTATCTAATAATATCGCCAGATAAAAAAAGGTTCTAT‑AATGAAAAAATTCCTATCGAATAAGAAGGGAGCATCAACCGTTGAGTTTGCCTTGACGGTTGTATTTTATTTCTTTGTCGTATTCCTAATTCTAGAATTCTGTCGTATATCTATTACAACTGCGTACTGGGACTTGGCTATCACTGAAAGTGCAAGAATTGCTAAAAATAGAACTGCAGAGGGTAATGATTATGCGGTAGAATTTGAGAAAGCTCTAAAACAACAGCTTGTTTATCAAGAATCTTCAACTTTAGGTTATCTCGCCCGTTTGGATAAAAATGGCGGTTACAAGATTGATGTTAAATATGTTGATTGTGGTAGTGAGAGTTCTTGTGTTAAGTCTTTATTGGATGGTAGGTTTAGACGGCCTACTAAAGATAGAGACGGTAAGATTATAC  >  1/1486764‑1487244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑