Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,486,913	T→C	F22F (TTT→TTC)	unknown →	Flp pilus assembly membrane protein TadE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,486,913	0	T	C	100.0%	144.8 / NA	51	F22F (TTT→TTC)	unknown	Flp pilus assembly membrane protein TadE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/28);  total (23/28)

GTTAGGGAACAGCGTTTAGTACACAATCTAATCTTTGCTTTAATCAAAAATAATGACATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAATACATCTCCTGATGATTTAATTAATGCTCTCAAAAAAACTGAACGCGTATCTAATAATATCGCCAGATAAAAAAAGGTTCTAT‑AATGAAAAAATTCCTATCGAATAAGAAGGGAGCATCAACCGTTGAGTTTGCCTTGACGGTTGTATTTTATTTCTTTGTCGTATTCCTAATTCTAGAATTCTGTCGTATATCTATTACAACTGCGTACTGGGACTTGGCTATCACTGAAAGTGCAAGAATTGCTAAAAATAGAACTGCAGAGGGTAATGATTATGCGGTAGAATTTGAGAAAGCTCTAAAACAACAGCTTGTTTATCAAGAATCTTCAACTTTAGGTTATCTCGCCCGTTTGGATAAAAATGGCGGTTACAAGATTG  >  1/1486666‑1487142                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                      gTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGtattctatt                                                                                                                                                                                                                                   >  1:247510/1‑251 (MQ=255)   tAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTc                                                                                                                                                                                                                                 <  1:190547/251‑1 (MQ=255)              gtttgatACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTAtt                                                                                                                                                                                                                      >  1:128473/7‑251 (MQ=255)               tttgatACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATACTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTc                                                                                                                                                                                                                     <  1:32267/246‑1 (MQ=255)                   atACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTATAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTaa                                                                                                                                                                                                                 >  1:785740/2‑251 (MQ=255)                        caATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGtattctattt                                                                                                                                                                                                                                  <  1:353629/229‑1 (MQ=255)                          atctagtctTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGa                                                                                                                                                                                                          >  1:124783/1‑251 (MQ=255)                          atctagtctTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGa                                                                                                                                                                                                          >  1:59036/1‑251 (MQ=255)                            ctagtctTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAAt                                                                                                                                                                                                        <  1:808387/247‑1 (MQ=255)                                            tCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTa                                                                                                                                                                                         <  1:167299/250‑1 (MQ=255)                                                 aataacGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTAcc                                                                                                                                                                                    <  1:209028/250‑2 (MQ=255)                                                 aataacGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTAcc                                                                                                                                                                                    <  1:59003/250‑2 (MQ=255)                                                 aataacGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTAcc                                                                                                                                                                                    <  1:87235/250‑2 (MQ=255)                                                 aataacGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTAc                                                                                                                                                                                     <  1:199123/249‑1 (MQ=255)                                                        aaaTTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGcat                                                                                                                                                                             <  1:786540/248‑3 (MQ=255)                                                            tGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGcatattgg                                                                                                                                                                        <  1:658225/251‑8 (MQ=255)                                                                         gATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGatttagccataa                                                                                                                                                           >  1:17258/1‑240 (MQ=255)                                                                                     aaGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCaa                                                                                                                                               >  1:447683/1‑251 (MQ=255)                                                                                        gAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAg                                                                                                                                              <  1:887285/249‑1 (MQ=255)                                                                                         aaaaCATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAgga                                                                                                                                            <  1:698914/250‑3 (MQ=255)                                                                                         aaaaCATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAgga                                                                                                                                            <  1:586392/250‑3 (MQ=255)                                                                                          aaaCATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTc                                                                                                                                                                                                      <  1:579971/191‑1 (MQ=255)                                                                                            aCATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTc                                                                                                                                                                                                                                 >  1:662458/1‑162 (MQ=255)                                                                                            aCATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCg                                                                                                                                                                                                                          <  1:190023/169‑1 (MQ=255)                                                                                             cATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAAtt                                                                                                                                                                                                               >  1:386116/1‑179 (MQ=255)                                                                                                   caCATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTaaaaac                                                                                                                                 <  1:494047/250‑2 (MQ=255)                                                                                                       tccccTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGaa                                                                                                                             >  1:35572/4‑251 (MQ=255)                                                                                                       tccccTGATGATTTAATTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTAcc                                                                                                                                                                                    <  1:14055/193‑2 (MQ=255)                                                                                                         cccTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAAt                                                                                                                                                                                                                >  1:343193/2‑166 (MQ=255)                                                                                                             gatgatTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAgga                                                                                                                                            <  1:462452/230‑3 (MQ=255)                                                                                                                        taatGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGAt                                                                                                            <  1:482743/251‑1 (MQ=255)                                                                                                                                            aaCGCGTGTCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTgaaaa                                                                                        <  1:423956/251‑4 (MQ=255)                                                                                                                                                   gtCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTAccac                                                                                                                                                                                  <  1:81610/153‑4 (MQ=255)                                                                                                                                                   gtCTAGTAATATCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGatttagcca                                                                                                                                                              <  1:857768/173‑9 (MQ=255)                                                                                                                                                      tagtaataTCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCtttat                                                                                                                                                                                                                            >  1:392053/4‑107 (MQ=255)                                                                                                                                                        gtaataTCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGcatatt                                                                                                                                                                          <  1:520550/156‑6 (MQ=255)                                                                                                                                                        gtaataTCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAgga                                                                                                                                            >  1:632849/2‑185 (MQ=255)                                                                                                                                                        gtaataTCACCAGAT‑‑AAAAAGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAaaca                                                                            >  1:179337/2‑251 (MQ=255)                                                                                                                                                                tcaccagataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTa                                                                                                                                                                                         >  1:288878/10‑136 (MQ=255)                                                                                                                                                                     agataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTg                                                                                                                                                  >  1:594033/5‑170 (MQ=255)                                                                                                                                                                      gataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTAccac                                                                                                                                                                                  <  1:355721/134‑4 (MQ=255)                                                                                                                                                                      gataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTAc                                                                                                                                                                                     >  1:340139/4‑134 (MQ=255)                                                                                                                                                                      gataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTgaaaa                                                                                        >  1:570457/4‑224 (MQ=255)                                                                                                                                                                      gataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTa                                                                >  1:596994/4‑251 (MQ=255)                                                                                                                                                                       ataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTgaaaa                                                                                        >  1:663824/3‑223 (MQ=255)                                                                                                                                                                       ataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACttatttat                                                               >  1:304702/3‑251 (MQ=255)                                                                                                                                                                       ataaaaaGGTTCTGTAAATGGAAAAATTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACttatttat                                                               >  1:138808/3‑251 (MQ=255)                                                                                                                                                                                            aaaaaTTCCTATCAAATAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAggc                                          <  1:335046/251‑2 (MQ=255)                                                                                                                                                                                                          aaTAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTc                                                                                                                                                                                                                     >  1:184918/1‑66 (MQ=255)                                                                                                                                                                                                          aaTAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACtat                                              <  1:371139/233‑3 (MQ=255)                                                                                                                                                                                                          aaTAAAAAGGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGttt                            >  1:111173/1‑251 (MQ=255)                                                                                                                                                                                                              aaaagGGAGCGTCAGCCGTTGAGTTTGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAgga                                                                                                                                            <  1:446240/133‑3 (MQ=255)                                                                                                                                                                                                                                     tttGCCTTGACGGTTGTATTCTATTTCTTTATCGTATTCTTAATTCTAGAATTCTGCCGTATATCTATTACCACTGCATATTGGGATTTAGCCATAACTGAAAGTGCAAGGATCGCTAAAAACAGAACGGCGGAAGGTAACGATTATGCTGCAGAATTTGAAAAAGCTCTAAAACAACAACTTATTTATCAAGAAACCTCAACTATAGGCTATCTTGCCCGTTTGGATAAAAATGGCGGTTatgaaattg  <  1:274106/250‑9 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                      GTTAGGGAACAGCGTTTAGTACACAATCTAATCTTTGCTTTAATCAAAAATAATGACATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAATACATCTCCTGATGATTTAATTAATGCTCTCAAAAAAACTGAACGCGTATCTAATAATATCGCCAGATAAAAAAAGGTTCTAT‑AATGAAAAAATTCCTATCGAATAAGAAGGGAGCATCAACCGTTGAGTTTGCCTTGACGGTTGTATTTTATTTCTTTGTCGTATTCCTAATTCTAGAATTCTGTCGTATATCTATTACAACTGCGTACTGGGACTTGGCTATCACTGAAAGTGCAAGAATTGCTAAAAATAGAACTGCAGAGGGTAATGATTATGCGGTAGAATTTGAGAAAGCTCTAAAACAACAGCTTGTTTATCAAGAATCTTCAACTTTAGGTTATCTCGCCCGTTTGGATAAAAATGGCGGTTACAAGATTG  >  1/1486666‑1487142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑