Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	282,391	A→G	Y7Y (TAT→TAC)	unknown ←	HesA/MoeB/ThiF family protein => sulfur transfer pathway protein CsdL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	282,391	0	A	G	100.0%	188.4 / NA	67	Y7Y (TAT→TAC)	unknown	HesA/MoeB/ThiF family protein => sulfur transfer pathway protein CsdL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/45);  total (22/45)

TAATGTCGGTTTTGAGTTGCCCGATATTACCGCTCAAGGCATGAATCTGGCGATTAATATTGGTAACACAAATATCATCCATATCAATCATCGTAATCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCATAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTGCCGTCATTATCTATTTGCGGCACCTCCACCAGCAACACGCTACCCAATGGAATCACAC  >  1/282153‑282628                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                tAATGTCGGTTTTGAGTTGCCCGATATTACCGCTCAAGGCATGAATCTGGCGATTAATATTGGTAACACAAATATCATCCATATCAATCATCGTAATCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑tg                                                                                                                                                                                                                                   <  1:790408/251‑1 (MQ=255) tAATGTCGGTTTTGAGTTGCCCGATATTACCGCTCAAGGCATGAATCTGGCGATTAATATTGGTAACACAAATATCATCCATATCAATCATCGTAATCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑tg                                                                                                                                                                                                                                   <  1:551769/251‑1 (MQ=255)           tttGAGTTGCCCGATATTACCGCTCAAGGCATGAATCTGGCGATTAATATTGGTAACACAAATATCATCCATATCAATCATCGTAATCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTa                                                                                                                                                                                                                         <  1:801192/251‑1 (MQ=255)           tttGAGTTGCCCGATATTACCGCTCAAGGCATGAATCTGGCGATTAATATTGGTAACACAAATATCATCCATATCAATCATCGTAATCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTa                                                                                                                                                                                                                         <  1:39839/251‑1 (MQ=255)                    cccGATATTACCGCTCAAGGCATGAATCTGGCGATTAATATTGGTAACACAAATATCATCCATATCAATCATCGTAATCTTGCCAATGCGGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAacac                                                                                                                                                                                                                <  1:570135/251‑1 (MQ=255)                       gATATTACCGCTCAAGGCATGAATCTGGCGATTAATATTGGTAACACAAATATCATCCATATCAATCATCGTAATCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACg                                                                                                                                                                                                                      >  1:510310/1‑242 (MQ=255)                           ttACCGCTCAAGGCATGAATCTGGCGATTAATATTGGTAACACAAATATCATCCATATCAATCATCGTAATCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAAcg                                                                                                                                                                                                         >  1:420199/1‑251 (MQ=255)                                     aGGCATGAATCTGGCGATTAATATTGGTAACACAAATATCATCCATATCAATCATCGTAATCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACAcc                                                                                                                                                                                                               >  1:667833/1‑235 (MQ=255)                                       gCATGAGTTTGGCGATTAATATTGGTAACAGAAATATCATCCATATCAGTCATCTTACACTTGCCGATGCGGGAACGGGCGAGAGCTTCGACCGCCAGGGAGCCAACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATGAACGTAACACCCAAACGCGACCGTAATg                                                                                                                                                                                              <  1:247748/250‑1 (MQ=255)                                                   cGATTAATATTGGTAACACAAATATCATCCATATCAATCATCGTAATCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCg                                                                                                                                                                                 >  1:625002/1‑251 (MQ=255)                                                                   cacaAATATCATCCATATCAATCATCGTAATCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTcc                                                                                                                                                                      <  1:773309/246‑1 (MQ=255)                                                                   cacaAATATCATCCATATCAATCATCGTAATCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTAACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATg                                                                                                                                                                                              <  1:730848/222‑1 (MQ=255)                                                                     caAATATCATCCATATCAATCATCGTAATCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGa                                                                                                                                                               >  1:825739/1‑251 (MQ=255)                                                                      aaaTATCATCCATATCAATCATCGTAATCTTGTCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGAt                                                                                                                                                              <  1:842564/251‑1 (MQ=255)                                                                               ccATATCAATCATCGTAATCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATAc                                                                                                                                                     <  1:721460/251‑1 (MQ=255)                                                                                 atatCAATCATCGTAATCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATAc                                                                                                                                                     >  1:200848/1‑249 (MQ=255)                                                                                    tcaatcaTCGTAATCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGa                                                                                                                                                <  1:240826/251‑1 (MQ=255)                                                                                    tcaatcaTCGTAATCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGa                                                                                                                                                <  1:570818/251‑1 (MQ=255)                                                                                       atcatcGTAATCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGatat                                                                                                                                             <  1:111294/251‑1 (MQ=255)                                                                                       atcatcGTAATCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGatat                                                                                                                                             <  1:757516/251‑1 (MQ=255)                                                                                       atcatcGTAATCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGatat                                                                                                                                             <  1:523018/251‑1 (MQ=255)                                                                                           tcGTAATCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAAt                                                                                                                                         >  1:575919/1‑251 (MQ=255)                                                                                                aTCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAAcg                                                                                                                                                                                                         <  1:51899/182‑1 (MQ=255)                                                                                                      ccggtGCCGGAACGGGCGAGAGCTTCCCCCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGgtgt                                                                                                                              <  1:140812/247‑1 (MQ=255)                                                                                                                          agCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑tg                                                                                                                                                                                                                                   <  1:459454/130‑1 (MQ=255)                                                                                                                              cccACCGCCCAGAAACGGACGCAGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCAATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTACCACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATcc                                                                                                      <  1:483236/250‑1 (MQ=255)                                                                                                                                     cccAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATcc                                                                                                      <  1:215488/244‑1 (MQ=255)                                                                                                                                          gAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCa                                                                                            <  1:522063/249‑1 (MQ=255)                                                                                                                                                     gccgATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTAATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAgcg                                                                                                  >  1:423458/1‑232 (MQ=255)                                                                                                                                                      ccgATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTg                                                                               <  1:484245/250‑1 (MQ=255)                                                                                                                                                              gATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATcc                                                                                                      >  1:327513/1‑219 (MQ=255)                                                                                                                                                                       aaTGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAacac                                                                                                                                                                                                                >  1:635745/1‑104 (MQ=255)                                                                                                                                                                        aTGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTg                                                            <  1:81036/251‑1 (MQ=255)                                                                                                                                                                        aTGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTg                                                            <  1:723554/251‑1 (MQ=255)                                                                                                                                                                              gTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTg                                                                               >  1:168421/1‑226 (MQ=255)                                                                                                                                                                               ttttACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAATGTGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGatat                                                                                                                                             >  1:156185/1‑165 (MQ=255)                                                                                                                                                                               ttttACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATc                                                                                                                                        <  1:715381/168‑1 (MQ=255)                                                                                                                                                                                 ttACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAgg                                                                                       <  1:722594/215‑1 (MQ=255)                                                                                                                                                                                 ttACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTGCCGTCATTa                                                   >  1:302996/1‑251 (MQ=255)                                                                                                                                                                                   aCGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTGCCGTCATTATc                                                 <  1:841789/251‑1 (MQ=255)                                                                                                                                                                                             gcTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATcc                                                                                                      <  1:759921/188‑1 (MQ=255)                                                                                                                                                                                              cTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAgg                                                                                       >  1:737663/1‑202 (MQ=255)                                                                                                                                                                                              cTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTGCCGTCATTATCTATTTGCgg                                        <  1:809937/249‑1 (MQ=255)                                                                                                                                                                                              cTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTGCCGTCATTATCTATTTGCGGc                                       <  1:582923/250‑1 (MQ=255)                                                                                                                                                                                                    cATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAat                                                                                                                                                                            >  1:585340/1‑111 (MQ=255)                                                                                                                                                                                                    cATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGatat                                                                                                                                             >  1:631762/1‑142 (MQ=255)                                                                                                                                                                                                    cATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGaaa                                                                                                                                    <  1:764565/151‑1 (MQ=255)                                                                                                                                                                                                       cGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTGCCGTCATTATCTATTTGCGGCACCTccacca                             <  1:411453/251‑1 (MQ=255)                                                                                                                                                                                                         gcgcCATATAAACGGCCGATACCGCGAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAATCGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTGCCGTCATTATCTATTTGCGGCACCTCCACCAg                            <  1:363656/250‑1 (MQ=255)                                                                                                                                                                                                         gcgcCATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTGCCGTCATTATCTATTTGCGGc                                       <  1:363533/239‑1 (MQ=255)                                                                                                                                                                                                            ccATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTGCCGTCATTATCTATTTGCGGCACCTCCACCAGCaa                         >  1:236367/1‑250 (MQ=255)                                                                                                                                                                                                             cATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAacac                                                                                                                                                                                                                >  1:658014/1‑66 (MQ=255)                                                                                                                                                                                                             cATATAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTg                                                                               >  1:567495/1‑195 (MQ=255)                                                                                                                                                                                                              atatAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATAc                                                                                                                                                     <  1:322660/124‑1 (MQ=255)                                                                                                                                                                                                              atatAAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATgg                                                                                                                                 <  1:138637/144‑1 (MQ=255)                                                                                                                                                                                                                ataAACGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTGCCGTCATTATCTATTTGCGGCACCTCCACCAGCAACACGc                    <  1:694671/251‑1 (MQ=255)                                                                                                                                                                                                                     cGGCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTGCCGTCATTATCTATTTGCGGCACCTCCACCAGCAACACGCTAccc               <  1:535956/251‑1 (MQ=255)                                                                                                                                                                                                                      ggCCGATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTGCCGTCATTATCTATTTGCGGCACCTCCACCAGCAACACGc                    >  1:354841/1‑245 (MQ=255)                                                                                                                                                                                                                          gATACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTg                                                                               <  1:633441/182‑1 (MQ=255)                                                                                                                                                                                                                           aTACCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATTGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTGCCGTCATTATCTATTTGCGGCACCTCCACCAGCAACACGCTACCCAATgg          <  1:355158/250‑1 (MQ=255)                                                                                                                                                                                                                             aCCGCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTGCCGTCATTATCTATTTGCGGCACCTCCACCAGCAACACGCTACCCAATGGAAt       <  1:495909/251‑1 (MQ=255)                                                                                                                                                                                                                                gCCAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTGCCGTCATTATCTATTTGCGGCACCTCCACCAGCAACACGCTACCCAATGGAATca     >  1:81150/1‑250 (MQ=255)                                                                                                                                                                                                                                gCCAAAGCGTTGTTCGTAGCTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTGCCGTCATTATCTATTTGCGGCACCTCCACCAGCAACACGCTACCCAATGGAATca     >  1:765324/1‑250 (MQ=255)                                                                                                                                                                                                                                 ccAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTGCCGTCATTATCTATTTGCGGCACCTCCACCAGCAACACGCTACCCAATGGAATcac    <  1:669395/250‑1 (MQ=255)                                                                                                                                                                                                                                  cAAAGCGTTGTTCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTGCCGTCATTATCTATTTGCGGCACCTCCACCAGCAACACGCTACCCAATGGAATCacac  <  1:485608/251‑1 (MQ=255)                                                                                                                                                                                                                                           gttCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTGCCGTCATTATCTATTTGCGGCACCTCCACCAGCAac                        <  1:118297/220‑1 (MQ=255)                                                                                                                                                                                                                                           gttCGTAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTGCCGTCATTATCTATTTGCGGCACCTCCACCAGCAACACGCTAccc               <  1:857197/229‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                TAATGTCGGTTTTGAGTTGCCCGATATTACCGCTCAAGGCATGAATCTGGCGATTAATATTGGTAACACAAATATCATCCATATCAATCATCGTAATCTTGCCGATGCCGGAACGGGCGAGAGCTTCCACCGCCCAGGAACCGACGCCGCCGATACCGATTACGCAAATGTGCGTTTTACGCAAGCGCGCTAAAGCATCGGCGCCATATAAACGGCCGATACCGCCAAAGCGTTGTTCATAGTTGTCAA‑‑TGTGCGCCATTAACGTAACACCCAAACGCGACCGTAATGTTTCGACAAGCCGGCAATATGTCCCGCCTGATCGCCGATACCGCGATATAAATCGAAATGGTGTCCGTTCACGGCACCGCCCACATCCAGCGCAACCATGAGGTGTAACTGATGTTGCCCTGTCCAGTTGCCGTCATTATCTATTTGCGGCACCTCCACCAGCAACACGCTACCCAATGGAATCACAC  >  1/282153‑282628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑