Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,486,614	C→T	N181N (AAC→AAT)	unknown →	Flp pilus assembly protein TadD, contains TPR repeat

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,486,614	0	C	T	100.0%	113.3 / NA	43	N181N (AAC→AAT)	unknown	Flp pilus assembly protein TadD, contains TPR repeat
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/22);  total (21/22)

GGCCAAAGTTCTACAAGCAAGAAATTTAAATCAACTCAAGCGCTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCCAAAAACGGGGAAGTTTATAATCTTCGTGGTGTGACTTATGCCTTAATGGGAAATCCTAATAAAGCCAACGAAGATATAAATAAAGCCCGCGAGTATTTTTTAAACGATGCTGTAGCGGTAAACAATCTTGCTATGCTAAGTATCGTCAACGGAGATTATCGTAATGCCGTTTCATTGTTATTACCTCAGTATTTAAATGGGGTTAGGGAACAGCGTTTAGTACACAATCTAATCTTTGCTTTAATCAAAAATAATGACATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAATACATCTCCTGATGATTTAATTAATGCTCTCAAAAAAACTGAACGCGTATCTAATAATATCGCCAGATAAAAAAA  >  1/1486374‑1486838                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                 ggCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTAt                                                                                                                                                                                                                         <  1:620741/250‑1 (MQ=255)   ccAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGt                                                                                                                                                                                                                      <  1:151741/251‑1 (MQ=255)   ccAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGt                                                                                                                                                                                                                      <  1:117556/251‑1 (MQ=255)      aaattCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAAt                                                                                                                                                                                                                   >  1:297629/4‑251 (MQ=255)        attCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGc                                                                                                                                                                                                                 <  1:858571/250‑1 (MQ=255)        attCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGc                                                                                                                                                                                                                 <  1:557515/250‑1 (MQ=255)                          ttAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCattattattgcc                                                                                                                                                                                               <  1:27851/251‑4 (MQ=255)                          ttAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCattattattgcc                                                                                                                                                                                               <  1:589520/251‑4 (MQ=255)                                  acttaaGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATtattat                                                                                                                                                                                                   <  1:566666/235‑1 (MQ=255)                                  acttaaGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTAtt                                                                                                                                                                                       >  1:572086/5‑251 (MQ=255)                                  acttaaGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTAtt                                                                                                                                                                                       >  1:802420/5‑251 (MQ=255)                                  acttaaGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTAtt                                                                                                                                                                                       >  1:266487/5‑251 (MQ=255)                                   cttaaGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTAttt                                                                                                                                                                                      <  1:829723/248‑1 (MQ=255)                                      aaGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTaaa                                                                                                                                                                                   <  1:844866/251‑1 (MQ=255)                                             aTCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCAtta                                                                                                                                                                                                       <  1:378780/224‑2 (MQ=255)                                             aTCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGtt                                                                                                                                                                            >  1:289089/1‑251 (MQ=255)                                               cAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGttt                                                                                                                                                                                                            >  1:784040/1‑217 (MQ=255)                                                     gCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCattattattgcc                                                                                                                                                                                               <  1:57228/224‑4 (MQ=255)                                                     gCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACa                                                                                                                                                                    >  1:73577/1‑251 (MQ=255)                                                                                                                                               aaTCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTaacac                                                                          <  1:276481/251‑4 (MQ=255)                                                                                                                                                aTCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAAcaca                                                                         >  1:702045/1‑251 (MQ=255)                                                                                                                                                         aaGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCtgat                                                                <  1:590839/251‑1 (MQ=255)                                                                                                                                                            cgaaCGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCtgatga                                                              <  1:243384/248‑1 (MQ=255)                                                                                                                                                              aaCGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAAc                                                                                  >  1:664567/1‑228 (MQ=255)                                                                                                                                                                    gATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATttagttaa                                                     >  1:675143/1‑251 (MQ=255)                                                                                                                                                                    gATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATttag                                                         <  1:558830/247‑2 (MQ=255)                                                                                                                                                                     atattaacaaagcacgagaatATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATttagtta                                                      <  1:888312/229‑1 (MQ=255)                                                                                                                                                                                     agaatATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCaagaaaa                                       >  1:358053/5‑248 (MQ=255)                                                                                                                                                                                      gaatATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTaacac                                                                          >  1:33921/4‑209 (MQ=255)                                                                                                                                                                                                      atgcagtggcagtaaataatatggctatgctaAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCtagtaa                    <  1:567661/227‑1 (MQ=255)                                                                                                                                                                                                      atgcagtggcagtaaataatatggctatgctaAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCtagtaa                    <  1:847819/227‑1 (MQ=255)                                                                                                                                                                                                       tgcagtggcagtaaataatatggctatgctaAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAAc                                      >  1:357793/23‑231 (MQ=255)                                                                                                                                                                                                             ggcagtaaataatatggctatgctaAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCa            >  1:616859/17‑251 (MQ=255)                                                                                                                                                                                                                 gtaaataatatggctatgctaAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACAt                                                                                >  1:469421/13‑179 (MQ=255)                                                                                                                                                                                                                 gtaaataatatggctatgctaAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGCCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGATa        >  1:635877/13‑251 (MQ=255)                                                                                                                                                                                                                   aaataatatggctatgctaAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACAtcccc                                                                    >  1:680980/11‑186 (MQ=255)                                                                                                                                                                                                                   aaataatatggctatgctaAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGATaaa      >  1:506514/11‑251 (MQ=255)                                                                                                                                                                                                                      taatatggctatgctaAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAAc                                      <  1:844816/209‑1 (MQ=255)                                                                                                                                                                                                                      taatatggctatgctaAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCtagtaa                    >  1:64324/8‑234 (MQ=255)                                                                                                                                                                                                                             gctatgctaAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCAAGAAAACTGAACGCGTGTCTAGTAATATCACCAGATaaaaagg  >  1:635640/1‑243 (MQ=255)                                                                                                                                                                                                                                atgctaAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGc                                                  <  1:567019/194‑1 (MQ=255)                                                                                                                                                                                                                                  gctaAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACAtcc                                                                      >  1:415678/1‑171 (MQ=255)                                                                                                                                                                                                                                  gctaAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATttag                                                         <  1:540000/185‑2 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                 GGCCAAAGTTCTACAAGCAAGAAATTTAAATCAACTCAAGCGCTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCCAAAAACGGGGAAGTTTATAATCTTCGTGGTGTGACTTATGCCTTAATGGGAAATCCTAATAAAGCCAACGAAGATATAAATAAAGCCCGCGAGTATTTTTTAAACGATGCTGTAGCGGTAAACAATCTTGCTATGCTAAGTATCGTCAACGGAGATTATCGTAATGCCGTTTCATTGTTATTACCTCAGTATTTAAATGGGGTTAGGGAACAGCGTTTAGTACACAATCTAATCTTTGCTTTAATCAAAAATAATGACATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAATACATCTCCTGATGATTTAATTAATGCTCTCAAAAAAACTGAACGCGTATCTAATAATATCGCCAGATAAAAAAA  >  1/1486374‑1486838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑