Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	277,038	A→G	T37A (ACA→GCA)	unknown →	SSU ribosomal protein S20p

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	277,038	0	A	G	100.0%	183.9 / NA	64	T37A (ACA→GCA)	unknown	SSU ribosomal protein S20p
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/27);  total (37/27)

TAAATATAACGATGCTTTCTTTCGGTTGTGTCTCACCGAAATCAACACAAAATTCCCGTTGACAAAAGTATAAGAAATCGGCATATTTACGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATACACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCAAAAAATTAGCATAATTTTTGCTTAGCAAATAAGGAGAAACCGCTCAATTGAGCGGTTTTTTGTTTTTATCAATTGTGTTTATTTCCGA  >  1/276791‑277267                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                      tAAATATAACGATGCTTTCTTTCGGTTGTGTCTCACCGAAATCAACACAAAATTCCCGTTGACAAAAGTATAAGAAATCGGCATATTTACGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATgcac                                                                                                                                                                                                                                    <  1:639778/251‑1 (MQ=255)    atatAACGATGCTTTCTTTCGGTTGTGTCTCACCGAAATCAACACAAAATTCCCGTTGACAAAAGTATAAGAAATCGGCATATTTACGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAg                                                                                                                                                                                                                                 >  1:659687/1‑251 (MQ=255)                              gtCTCACCGAAATCAACACAAAATTCCCGTTGACAAAAGTATAAGAAATCGGCATATTTACGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAgctg                                                                                                                                                                                                         >  1:647015/1‑249 (MQ=255)                               tctcACCGAAATCAACACAAAATTCCCGTTGACAAAAGTATAAGAAATCGGCATATTTACGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGctgctg                                                                                                                                                                                                      <  1:594677/251‑1 (MQ=255)                                   aCCGAAATCAACACAAAATTCCCGTTGACAAAAGTATAAGAAATCGGCATATTTACGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGc                                                                                                                                                                                                  <  1:386436/251‑1 (MQ=255)                                    ccGAAATCAACACAAAATTCCCGTTGACAAAAGTATAAGAAATCGGCATATTTACGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGcc                                                                                                                                                                                                 <  1:393543/251‑1 (MQ=255)                                                             gACAAAAGTATAAGAAATCGGCATATTTACGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTtg                                                                                                                                                                        >  1:331190/1‑251 (MQ=255)                                                                  aaGTATAAGAAATCGGCATATTTACGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGcc                                                                                                                                                                                                 <  1:458512/221‑1 (MQ=255)                                                                  aaGTATAAGAAATCGGCATATTTACGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGAc                                                                                                                                                                   <  1:889124/251‑1 (MQ=255)                                                                    gTATAAGAAATCGGCATATTTACGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAagcag                                                                                                                                                                                                            >  1:354895/1‑208 (MQ=255)                                                                    gTATAAGAAATCGGCATATTTACGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAg                                                                                                                                                                 >  1:800690/1‑251 (MQ=255)                                                                    gTATAAGAAATCGGCATATTTACGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAg                                                                                                                                                                 >  1:380094/1‑251 (MQ=255)                                                                                 gCATATTTACGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTCGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTaa                                                                                                                                                     <  1:598142/250‑1 (MQ=255)                                                                                   atatTTACGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAgg                                                                                                                                                  <  1:384737/251‑1 (MQ=255)                                                                                   atatTTACGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAgg                                                                                                                                                  <  1:611417/251‑1 (MQ=255)                                                                                     atTTACGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGtt                                                                                                                                                >  1:532348/1‑251 (MQ=255)                                                                                     atTTACGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGtt                                                                                                                                                >  1:118714/1‑251 (MQ=255)                                                                                      tttACGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGttt                                                                                                                                               >  1:888068/1‑251 (MQ=255)                                                                                       ttACGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTa                                                                                                                                              >  1:226248/1‑251 (MQ=255)                                                                                          cGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATc                                                                                                                                           <  1:271321/251‑1 (MQ=255)                                                                                          cGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATc                                                                                                                                           <  1:422724/251‑1 (MQ=255)                                                                                              ccTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGAc                                                                                                                                                                   >  1:674622/1‑223 (MQ=255)                                                                                              ccTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCAcg                                                                                                                                       >  1:547023/1‑251 (MQ=255)                                                                                                 aTTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGa                                                                                                                                    >  1:96673/1‑251 (MQ=255)                                                                                                        cACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAagc                                                                                                                             <  1:731387/251‑1 (MQ=255)                                                                                                          cTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAgg                                                                                                                                                  <  1:230144/228‑1 (MQ=255)                                                                                                          cTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAagcag                                                                                                                           >  1:384862/1‑251 (MQ=255)                                                                                                          cTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAagcag                                                                                                                           >  1:411023/1‑251 (MQ=255)                                                                                                                           tttttAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTaaa                                                                                                          <  1:835609/251‑1 (MQ=255)                                                                                                                            ttttAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAagcagc                                                                                                                                                                                                           >  1:453472/1‑153 (MQ=255)                                                                                                                             tttAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAAtt                                                                                                        <  1:544404/251‑1 (MQ=255)                                                                                                                                  gAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAg                                                                                                      >  1:513631/1‑248 (MQ=255)                                                                                                                                    gTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGcc                                                                                                                                                                                                 <  1:228700/155‑1 (MQ=255)                                                                                                                                        gACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTc                                                                                                                                                                                           >  1:127675/1‑157 (MQ=255)                                                                                                                                         aCCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATc                                                                                                                                                        <  1:464769/191‑1 (MQ=255)                                                                                                                                              ggCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCaaaa                                                                                       >  1:821796/1‑251 (MQ=255)                                                                                                                                                   atatCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATc                                                                                                                                           >  1:674603/1‑194 (MQ=255)                                                                                                                                                      tCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCAAAAAATTAGCa                                                                               <  1:158478/251‑1 (MQ=255)                                                                                                                                                                                              ccGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCAAAAAATTAGCATAATTTTTGCTTAGCAAATAAGGAGAAACCGCTCAATTGa                                       >  1:167324/1‑251 (MQ=255)                                                                                                                                                                                                gTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCAAAAAATTAGCATAATTTTTGCTTAGCAAATAAGGAGAAACCGCTCAATTGAg                                      >  1:266132/1‑250 (MQ=255)                                                                                                                                                                                                    acacAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAAc                                                                                                                      >  1:70855/1‑166 (MQ=255)                                                                                                                                                                                                    acacAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCAAAAAATTAGCATAATTTTTGCTTAGCAAATAAGGAGAAACCGCTCAATTGAGCGGtt                                 >  1:521709/1‑251 (MQ=255)                                                                                                                                                                                                              aGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCAAAAAATTAGCATAATTTTTGCTTAGCAAATAAGGAGAAACCGCTCAATTGAGCGGTTTTTTGttttt                       >  1:255346/1‑251 (MQ=255)                                                                                                                                                                                                               gCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGt                                                                                                                                                                          >  1:240732/1‑103 (MQ=255)                                                                                                                                                                                                               gCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATc                                                                                           <  1:523060/182‑1 (MQ=255)                                                                                                                                                                                                                     gCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATc                                                                                                              <  1:525891/157‑1 (MQ=255)                                                                                                                                                                                                                     gCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGc                                                                                                     <  1:773267/166‑1 (MQ=255)                                                                                                                                                                                                                     gCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGGACAAATCAAAAAATTAGCATAATTTTTGCTTAGCAAATAAGGAGAAACCGCTCAATTGAGCGGTTTTTTGTTTTTATCAAtt                >  1:4306/1‑251 (MQ=255)                                                                                                                                                                                                                     gCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCAAAAAATTAGCATAATTTTTGCTTAGCAAATAAGGAGAAACCGCTCAATTGAGCGGTTTTTTGTTTTTATCAAtt                >  1:573963/1‑251 (MQ=255)                                                                                                                                                                                                                     gCTCTATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCAAAAAATTAGCATAATTTTTGCTTAGCAAATAAGGAGAAACCGCTCAATTGAGCGGTTTTTTGTTTTTATCAAtt                >  1:241987/1‑251 (MQ=255)                                                                                                                                                                                                                      ctctATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCAAAAAATTAGCATAATTTTTGCTTAGCAAATAAGGAGAAACCGCTCAAt                                          >  1:484711/1‑224 (MQ=255)                                                                                                                                                                                                                      ctctATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCAAAAAATTAGCATAATTTTTGCTTAGCAAATAAGGAGAAACCGCTCAATTGAGCGGTTTTTTGTTTTTATCAATtg               <  1:591395/251‑1 (MQ=255)                                                                                                                                                                                                                        ctATGATGCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCAAAAAATTAGCATAATTTTTGCTTAGCAAATAAGGAGAAACCGCTCAATTGAGCGGTTTTTTGtt                          <  1:290906/238‑1 (MQ=255)                                                                                                                                                                                                                          atgatgCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCAAAAAATTAGCATAATTTTTGCTTAGCAAATAAGGAGAAAcc                                                 <  1:634701/213‑1 (MQ=255)                                                                                                                                                                                                                           tgatgCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCAAAAAATTAGCATAATTTTTGCTTAGCAAATAAGGAGAAACCGCTCAATTGAGCGGTTTTTTGTTTTTATCAATTGTGttt          <  1:268993/251‑1 (MQ=255)                                                                                                                                                                                                                              tgCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCAAAAAATTAGCATAATTTTTGc                                                                     >  1:11494/1‑189 (MQ=255)                                                                                                                                                                                                                              tgCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCAAAAAATTAGCATAATTTTTGCTTAGCAAATAAGGAGAAACCGCTCAAt                                          <  1:201938/216‑1 (MQ=255)                                                                                                                                                                                                                              tgCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCAAAAAATTAGCATAATTTTTGCTTAGCAAATAAGGAGAAACCGCTCAATTGAGCGGTTTTTTGTTTTTATCAATTGTGTTTAtt       >  1:634121/1‑251 (MQ=255)                                                                                                                                                                                                                               gCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCaaaaa                                                                                      >  1:713438/1‑171 (MQ=255)                                                                                                                                                                                                                               gCGTACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCAAAAAATTAGCATAATTTTTGCTTAGCAAATAAGGAGAAACCGCTCAATTGAGCGGTTTTTTGTTTTTATCAATTGTGTTTAttt      >  1:404994/1‑251 (MQ=255)                                                                                                                                                                                                                                  tACTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCAAAAAATTAGCATAATTTTTGCTTAGCAAATAAGGAGAAAcc                                                 <  1:171474/205‑1 (MQ=255)                                                                                                                                                                                                                                   aCTTACATCAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCAAAAAATTAGCATAATTTTTGCTTAGCAAATAAGGAGAAACCGCTCAATTGAGCGGTTTTTTGTTTTTATCAATTGTGTTTATTTCCGa  >  1:701794/1‑251 (MQ=255)                                                                                                                                                                                                                                           cAAAAAAGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATc                                                                                                                                           >  1:772435/1‑106 (MQ=255)                                                                                                                                                                                                                                                 aGTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAAtt                                                                                                        <  1:786298/135‑1 (MQ=255)                                                                                                                                                                                                                                                  gTTTATGCACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCAAAAAATTAGCATAATTTTTGCTTAGCAAATAAgg                                                        >  1:361395/1‑182 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                      TAAATATAACGATGCTTTCTTTCGGTTGTGTCTCACCGAAATCAACACAAAATTCCCGTTGACAAAAGTATAAGAAATCGGCATATTTACGCCCCTATTTTGTCACTATTAACTAACAGAAATTTTTAGGAGTTTGACCTTGGCTAATATCAAGTCAGCAAAAAAACGTGCGGTTCAATCTGAAAAACGCCGTCAACACAACGCAAGCCAACGCTCTATGATGCGTACTTACATCAAAAAAGTTTATACACAAGTTGCCGCAGGCGAGAAAGCAGCTGCTGAAGCCGCATTCGTTGAAATGCAAAAAGTTGTGGACAGAATGGCATCTAAAGGTTTAATCCACGCGAACAAAGCAGCCAACCACAAATCTAAATTAGCTGCACAAATCAAAAAATTAGCATAATTTTTGCTTAGCAAATAAGGAGAAACCGCTCAATTGAGCGGTTTTTTGTTTTTATCAATTGTGTTTATTTCCGA  >  1/276791‑277267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑