Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,486,569	C→T	N166N (AAC→AAT)	unknown →	Flp pilus assembly protein TadD, contains TPR repeat

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,486,569	0	C	T	100.0%	145.4 / NA	55	N166N (AAC→AAT)	unknown	Flp pilus assembly protein TadD, contains TPR repeat
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/34);  total (21/34)

CTACTCTATTTACAGCCGTTGCTAACAAACAGTGGGCAGTTAAGTGAAAAGGCCAAAGTTCTACAAGCAAGAAATTTAAATCAACTCAAGCGCTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCCAAAAACGGGGAAGTTTATAATCTTCGTGGTGTGACTTATGCCTTAATGGGAAATCCTAATAAAGCCAACGAAGATATAAATAAAGCCCGCGAGTATTTTTTAAACGATGCTGTAGCGGTAAACAATCTTGCTATGCTAAGTATCGTCAACGGAGATTATCGTAATGCCGTTTCATTGTTATTACCTCAGTATTTAAATGGGGTTAGGGAACAGCGTTTAGTACACAATCTAATCTTTGCTTTAATCAAAAATAATGACATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAATACATCTCCTGATGATTTAATTAATGCTCTCAAAAAAA  >  1/1486324‑1486801                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         ctactctactTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAatgatg                                                                                                                                                                                                                                      >  1:671526/1‑250 (MQ=255)   actctaCTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGcag                                                                                                                                                                                                                                   <  1:173912/251‑3 (MQ=255)        acttACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGcagtggca                                                                                                                                                                                                                              >  1:652437/3‑244 (MQ=255)         cttACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGcagtggcag                                                                                                                                                                                                                             >  1:674347/2‑243 (MQ=255)                ccGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTaaat                                                                                                                                                                                                                        <  1:854547/249‑2 (MQ=255)                ccGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGCAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTaaat                                                                                                                                                                                                                        <  1:784591/249‑2 (MQ=255)                    tGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAaataatatg                                                                                                                                                                                                                  >  1:23537/1‑248 (MQ=255)                     gCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTaaat                                                                                                                                                                                                                        <  1:352841/244‑2 (MQ=255)                      cTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAaataatatggc                                                                                                                                                                                                                <  1:364789/251‑6 (MQ=255)                      cTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAaataatatggc                                                                                                                                                                                                                <  1:620879/251‑6 (MQ=255)                        aacaaacaGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAaataatatggcta                                                                                                                                                                                                              <  1:568789/251‑8 (MQ=255)                        aacaaacaGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAaataatatggc                                                                                                                                                                                                                >  1:691556/1‑244 (MQ=255)                                 cgggCAGTTAAGTGAAAGGGCCAAAAGTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATggctatgcta                                                                                                                                                                                                         <  1:157384/246‑1 (MQ=255)                                   ggCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTAt                                                                                                                                                                                                    <  1:826444/250‑1 (MQ=255)                                   ggCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTAt                                                                                                                                                                                                    <  1:625293/250‑1 (MQ=255)                                       gTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAaataatatggct                                                                                                                                                                                                               >  1:362741/1‑229 (MQ=255)                                       gTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATcatta                                                                                                                                                                                               >  1:535635/1‑247 (MQ=255)                                       gTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATcatta                                                                                                                                                                                               >  1:704594/1‑247 (MQ=255)                                        ttAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTCCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATcatta                                                                                                                                                                                               <  1:18251/250‑5 (MQ=255)                                                   ggCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTa                                                                                                                                                                                                     <  1:522092/233‑1 (MQ=255)                                                   ggCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTAt                                                                                                                                                                                    <  1:620741/250‑1 (MQ=255)                                                     ccAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGt                                                                                                                                                                                 <  1:151741/251‑1 (MQ=255)                                                     ccAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGt                                                                                                                                                                                 <  1:117556/251‑1 (MQ=255)                                                        aaattCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAAt                                                                                                                                                                              >  1:297629/4‑251 (MQ=255)                                                          attCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGc                                                                                                                                                                            <  1:858571/250‑1 (MQ=255)                                                          attCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGc                                                                                                                                                                            <  1:557515/250‑1 (MQ=255)                                                                            ttAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCattattattgcc                                                                                                                                                          <  1:589520/251‑4 (MQ=255)                                                                            ttAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCattattattgcc                                                                                                                                                          <  1:27851/251‑4 (MQ=255)                                                                                    acttaaGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAaataatatggcta                                                                                                                                                                                                              <  1:270611/187‑8 (MQ=255)                                                                                    acttaaGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATggctatgct                                                                                                                                                                                                          <  1:585531/191‑1 (MQ=255)                                                                                    acttaaGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATtattat                                                                                                                                                              <  1:566666/235‑1 (MQ=255)                                                                                    acttaaGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTAtt                                                                                                                                                  >  1:572086/5‑251 (MQ=255)                                                                                    acttaaGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTAtt                                                                                                                                                  >  1:802420/5‑251 (MQ=255)                                                                                    acttaaGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTAtt                                                                                                                                                  >  1:266487/5‑251 (MQ=255)                                                                                     cttaaGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTAttt                                                                                                                                                 <  1:829723/248‑1 (MQ=255)                                                                                        aaGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTaaa                                                                                                                                              <  1:844866/251‑1 (MQ=255)                                                                                               aTCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGcagtgg                                                                                                                                                                                                                                <  1:718475/162‑6 (MQ=255)                                                                                               aTCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAaataatat                                                                                                                                                                                                                   >  1:122295/1‑173 (MQ=255)                                                                                               aTCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCAtta                                                                                                                                                                  <  1:378780/224‑2 (MQ=255)                                                                                               aTCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGtt                                                                                                                                       >  1:289089/1‑251 (MQ=255)                                                                                               aTCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCGGCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGATTGTTTCTTAAATGATGCAGTGGCAGTAaataatat                                                                                                                                                                                                                   <  1:796776/175‑3 (MQ=255)                                                                                                 cAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGttt                                                                                                                                                                       >  1:784040/1‑217 (MQ=255)                                                                                                       gCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCattattattgcc                                                                                                                                                          <  1:57228/224‑4 (MQ=255)                                                                                                       gCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACa                                                                                                                               >  1:73577/1‑251 (MQ=255)                                                                                                                gTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAGTGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATc                                                                                                                                                                                                   >  1:365875/1‑174 (MQ=255)                                                                                                                gTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGCTTTAATGGGCAATCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGt                                                                                                                                                                                                      <  1:865588/171‑1 (MQ=255)                                                                                                                                                                                                 aaTCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTaacac                                     <  1:276481/251‑4 (MQ=255)                                                                                                                                                                                                  aTCCAAATAAAGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAAcaca                                    >  1:702045/1‑251 (MQ=255)                                                                                                                                                                                                           aaGCGAACGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCtgat                           <  1:590839/251‑1 (MQ=255)                                                                                                                                                                                                              cgaaCGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCtgatga                         <  1:243384/248‑1 (MQ=255)                                                                                                                                                                                                                aaCGAAGATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAAc                                             >  1:664567/1‑228 (MQ=255)                                                                                                                                                                                                                      gATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATttagttaa                >  1:675143/1‑251 (MQ=255)                                                                                                                                                                                                                      gATATTAACAAAGCACGAGAATATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATttag                    <  1:558830/247‑2 (MQ=255)                                                                                                                                                                                                                       atattaacaaagcacgagaatATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATttagtta                 <  1:888312/229‑1 (MQ=255)                                                                                                                                                                                                                                       agaatATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAACACATCCCCTGATGATTTAGTTAATGCCCTCaagaaaa  >  1:358053/5‑248 (MQ=255)                                                                                                                                                                                                                                        gaatATTTCTTAAATGATGCAGTGGCAGTAAATAATATGGCTATGCTAAGTATCATTAATGGAGATTATCGTAATGCTGTTTCATTATTATTGCCTCAGTATTTAAATGGAGTTAGGGAACAGCGTTTGATACACAATCTAGTCTTTGCTTTAGTCAAGAATAACGAAATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTaacac                                     >  1:33921/4‑209 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         CTACTCTATTTACAGCCGTTGCTAACAAACAGTGGGCAGTTAAGTGAAAAGGCCAAAGTTCTACAAGCAAGAAATTTAAATCAACTCAAGCGCTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCCAAAAACGGGGAAGTTTATAATCTTCGTGGTGTGACTTATGCCTTAATGGGAAATCCTAATAAAGCCAACGAAGATATAAATAAAGCCCGCGAGTATTTTTTAAACGATGCTGTAGCGGTAAACAATCTTGCTATGCTAAGTATCGTCAACGGAGATTATCGTAATGCCGTTTCATTGTTATTACCTCAGTATTTAAATGGGGTTAGGGAACAGCGTTTAGTACACAATCTAATCTTTGCTTTAATCAAAAATAATGACATTGATTACGCAAAAGATATTATTGTGAAGGAAAACATTAATACATCTCCTGATGATTTAATTAATGCTCTCAAAAAAA  >  1/1486324‑1486801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑