Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,486,272	A→G	T67T (ACA→ACG)	unknown →	Flp pilus assembly protein TadD, contains TPR repeat

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,486,272	0	A	G	100.0%	240.1 / NA	84	T67T (ACA→ACG)	unknown	Flp pilus assembly protein TadD, contains TPR repeat
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/39);  total (45/39)

GCGTAATGAGGGTATTTCCACATGTATTCTAAATCTCTTAAAAATGTTCTATTGTGCGGCATGATCTTATCTGTTACTGCATGTTCTGCTGTACTCAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGTACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACACGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAATTCATCTCTACTCTATTTACAGCCGTTGCTAACAAACAGTGGGCAGTTAAGTGAAAAGGCCAAAGTTCTACAAGCAAGAAATTTAAATCAACTCAAGCGCTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCCAAAAACGGGGAAGTTTATAATCTTCGTGGTGTGACTTATGCC  >  1/1486051‑1486506                                                                                                                                                                                                                              |                                                                                                                                                                                                                                           gtgTAATGAGGGTATTTCCACATGTATTCTAAATCTCTTAAAAATGTTCTATTATGCGGCATGATCTTATCTGTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTAc                                                                                                                                                                                                                 <  1:895961/247‑1 (MQ=255)          gggTATTTCCACATGTATTCTAAATCTCTTAAAAATGTTCTATTATGCGGCATGATCTTATCTGTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGa                                                                                                                                                                                                      >  1:75869/1‑251 (MQ=255)                           ttCTAAATCTCTTAAAAATGTTCTATTATGCGGCATGATCTTATCTGTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCGCGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATctcta                                                                                                                                                                                      >  1:449320/1‑250 (MQ=255)                           ttCTAAATCTCTTAAAAATGTTCTATTATGCGGCATGATCTTATCTGTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTc                                                                                                                                                                                             <  1:46744/243‑1 (MQ=255)                           ttCTAAATCTCTTAAAAATGTTCTATTATGCGGCATGATCTTATCTGTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATctcta                                                                                                                                                                                      >  1:779480/1‑250 (MQ=255)                             cTAAATCTCTTAAAAATGTTCTATTATGCGGCATGATCTTATCTGTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATctctactc                                                                                                                                                                                   <  1:26574/251‑1 (MQ=255)                                     ctTAAAAATGTTCTATTATGCGGCATGATCTTATCTGTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACa                                                                                                                                                                           >  1:145183/1‑251 (MQ=255)                                              gTTCTATTATGCGGCATGATCTTATCTGTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGc                                                                                                                                                                         >  1:112687/1‑244 (MQ=255)                                                 cTATTATGCGGCATGATCTTATCTGTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTAc                                                                                                                                                                                                                 >  1:771240/1‑201 (MQ=255)                                                 cTATTATGCGGCATGATCTTATCTGTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTc                                                                                                                                                                                             <  1:505275/221‑1 (MQ=255)                                                   attatgCGGCATGATCTTATCTGTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATaaa                                                                                                                                                                                                                                   <  1:529295/177‑1 (MQ=255)                                                   attatgCGGCATGATCTTATCTGTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGc                                                                                                                                                                         >  1:701171/5‑239 (MQ=255)                                                   attatgCGGCATGATCTTATCTGTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTaacaa                                                                                                                                                             >  1:100346/5‑251 (MQ=255)                                                          ggCATGATCTTATCTGTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATctctactctac                                                                                                                                                                                >  1:826923/1‑224 (MQ=255)                                                            cATGATCTTATCTGTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGc                                                                                                                                                    <  1:245515/251‑1 (MQ=255)                                                               gATCTTATCTGTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGt                                                                                                                                                 >  1:887131/1‑251 (MQ=255)                                                                 tCTTATCTGTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATaaa                                                                                                                                                                                                                                   >  1:212542/1‑167 (MQ=255)                                                                 tCTTATCTGTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTa                                                                                                                                               >  1:385054/1‑251 (MQ=255)                                                                 tCTTATCTGTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTa                                                                                                                                               >  1:620848/1‑251 (MQ=255)                                                                       cTGTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGaa                                                                                                                                         >  1:662157/1‑251 (MQ=255)                                                                       cTGTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGaa                                                                                                                                         >  1:460884/1‑251 (MQ=255)                                                                         gTTACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGaaag                                                                                                                                       >  1:700887/1‑250 (MQ=255)                                                                          ttACTGCATGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGaaagg                                                                                                                                      <  1:648776/251‑3 (MQ=255)                                                                                  tGTTCTGCTGTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCaaaat                                                                                                                              <  1:791705/251‑3 (MQ=255)                                                                                        gctgTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAAATATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGa                                                                                                                                          <  1:346068/233‑1 (MQ=255)                                                                                           gTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATaaa                                                                                                                                                                                                                                   <  1:132451/141‑1 (MQ=255)                                                                                           gTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAAt                                                                                                                                                                                                                                  <  1:358929/142‑1 (MQ=255)                                                                                           gTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGa                                                                                                                                          >  1:267253/1‑230 (MQ=255)                                                                                           gTACTAAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTAcaag                                                                                                                      >  1:900333/1‑250 (MQ=255)                                                                                                aaaTAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATctctactc                                                                                                                                                                                   <  1:727457/183‑1 (MQ=255)                                                                                                aaaTAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGaa                                                                                                                >  1:886743/2‑251 (MQ=255)                                                                                                           cATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCaa                                                                                                     >  1:128139/1‑251 (MQ=255)                                                                                                            aTTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCaa                                                                                                     >  1:80548/1‑250 (MQ=255)                                                                                                                 gTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAg                                                                                               >  1:599169/1‑251 (MQ=255)                                                                                                                    cTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAgcg                                                                                             <  1:736743/250‑1 (MQ=255)                                                                                                                                ctgctAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCaagaag                                                                                  <  1:287333/249‑1 (MQ=255)                                                                                                                                    tAAAGAAGCACTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTgg                                                                            <  1:881516/251‑1 (MQ=255)                                                                                                                                              cTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATaaa                                                                                                                                                                                                                                   >  1:672667/1‑90 (MQ=255)                                                                                                                                              cTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTa                                                                                                                                                                             <  1:235941/144‑1 (MQ=255)                                                                                                                                              cTTTATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAg                                                                         >  1:328193/1‑244 (MQ=255)                                                                                                                                               tttATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAAGTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAAc                                                                 <  1:186373/251‑1 (MQ=255)                                                                                                                                                 tATCAGAGCACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAg                                                               >  1:345450/1‑251 (MQ=255)                                                                                                                                                        gcaCTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATctctactc                                                                                                                                                                                   <  1:552797/126‑1 (MQ=255)                                                                                                                                                         caCTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCttatt                                                         <  1:712451/248‑1 (MQ=255)                                                                                                                                                           cTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGa                                                                                                                                          >  1:385799/1‑166 (MQ=255)                                                                                                                                                             aacaacaacGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAgcg                                                                                             >  1:731893/1‑209 (MQ=255)                                                                                                                                                             aacaacaacGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTaacaac                                                   <  1:355572/251‑1 (MQ=255)                                                                                                                                                               caacaacGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACAt                                                 >  1:535511/1‑251 (MQ=255)                                                                                                                                                               caacaacGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACAt                                                 >  1:575283/1‑251 (MQ=255)                                                                                                                                                               caacaacGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACAt                                                 >  1:220643/1‑251 (MQ=255)                                                                                                                                                                    acGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCaaga                                                                                    >  1:401167/1‑211 (MQ=255)                                                                                                                                                                     cGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTccta                                           <  1:568396/251‑3 (MQ=255)                                                                                                                                                                         gCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGt                                                                                                                                                                                                                                         >  1:863512/1‑57 (MQ=255)                                                                                                                                                                         gCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATc                                                                                                       <  1:613271/187‑1 (MQ=255)                                                                                                                                                                          cGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTaaaaa                                       <  1:84497/250‑1 (MQ=255)                                                                                                                                                                           gTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCAtc                                                                                                                                                                                          >  1:392961/1‑102 (MQ=255)                                                                                                                                                                           gTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACg                                     >  1:326379/1‑251 (MQ=255)                                                                                                                                                                              aaTCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACggtg                                  >  1:505240/1‑249 (MQ=255)                                                                                                                                                                              aaTCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACggtg                                  >  1:829543/1‑249 (MQ=255)                                                                                                                                                                                 cACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAg                               >  1:588992/1‑251 (MQ=255)                                                                                                                                                                                   cTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAgcg                                                                                             <  1:502687/187‑1 (MQ=255)                                                                                                                                                                                   cTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGt                              >  1:185423/1‑250 (MQ=255)                                                                                                                                                                                   cTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGt                              >  1:218674/1‑250 (MQ=255)                                                                                                                                                                                     aTGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTc                                                                                                                                                                                             <  1:614951/89‑1 (MQ=255)                                                                                                                                                                                     aTGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAgcg                                                                                             >  1:395833/1‑185 (MQ=255)                                                                                                                                                                                       gTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACt                                                                                                   >  1:712612/1‑177 (MQ=255)                                                                                                                                                                                       gTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAgcg                                                                                             <  1:37904/183‑1 (MQ=255)                                                                                                                                                                                       gTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCtata                         >  1:625282/1‑251 (MQ=255)                                                                                                                                                                                         aTCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAAt                       <  1:867545/251‑1 (MQ=255)                                                                                                                                                                                          tCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATc                      <  1:805879/251‑1 (MQ=255)                                                                                                                                                                                          tCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATc                      <  1:820390/251‑1 (MQ=255)                                                                                                                                                                                          tCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATc                      <  1:738663/251‑1 (MQ=255)                                                                                                                                                                                          tCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATc                      <  1:736431/251‑1 (MQ=255)                                                                                                                                                                                          tCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATc                      <  1:130407/251‑1 (MQ=255)                                                                                                                                                                                            gTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACggtga                                 <  1:61582/238‑4 (MQ=255)                                                                                                                                                                                            gTGATGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCtt                    >  1:734340/1‑251 (MQ=255)                                                                                                                                                                                               aTGGACTTAAAAATAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCg                  <  1:328006/250‑1 (MQ=255)                                                                                                                                                                                                        aaaaTAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGgtgtaacc        <  1:356027/251‑5 (MQ=255)                                                                                                                                                                                                        aaaaTAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGgtgtaacc        <  1:596161/251‑5 (MQ=255)                                                                                                                                                                                                         aaaTAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGAc                                                                                                                                                                                                     <  1:364303/61‑1 (MQ=255)                                                                                                                                                                                                         aaaTAAAGAAGATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGgtgtaacct       <  1:784453/251‑6 (MQ=255)                                                                                                                                                                                                              aagaagATCCTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAATCTTCGTGGTGTAACCTATGct  <  1:110660/251‑2 (MQ=255)                                                                                                                                                                                                                      ccTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGa                                                                                                                                          >  1:214637/1‑107 (MQ=255)                                                                                                                                                                                                                      ccTCTCACGCGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAGTTCATCTCTACTCTACTTACAGCCGTTGCTAACAAACAGCGGGCAGTTAAGTGAAAGGGCCAAAATTCTACAAGCACGAAATTTAAATCAACTTAAGCGTTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCTAAAAACGGTGAAGTCTATAAt                       >  1:620149/1‑222 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                           GCGTAATGAGGGTATTTCCACATGTATTCTAAATCTCTTAAAAATGTTCTATTGTGCGGCATGATCTTATCTGTTACTGCATGTTCTGCTGTACTCAATAAAAAACCATTAAGTGCTGATAAATTAGCTGCTAAAGAAGCACTTTATCAGAGTACTAACAACAACGATGCGTTAATCACTATGTATCGTGATGGACTTAAAAATAAAGAAGATCCTCTCACACGTTATAAATTATCTGAAATTTATTACAAGAAAGGGGACAGTAATTCATCTCTACTCTATTTACAGCCGTTGCTAACAAACAGTGGGCAGTTAAGTGAAAAGGCCAAAGTTCTACAAGCAAGAAATTTAAATCAACTCAAGCGCTATCAAGAAGCGTTGGAAGTCGAAAACAGCTTATTAACAACATCTCCCAAAAACGGGGAAGTTTATAATCTTCGTGGTGTGACTTATGCC  >  1/1486051‑1486506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑