Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,485,845	G→A	T210A (ACG→GCA)	unknown →	Type II/IV secretion system protein TadC, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,485,845	0	G	A	100.0%	137.2 / NA	53	T210A (ACG→GCA)	unknown	Type II/IV secretion system protein TadC, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/21);  total (32/21)

ATTGATTTAGTAGCTGTTAATGTACAAACAGGGATAAGTATTGAGGCCGCACTAAAACAGGTTGCAACTGACTTTAAAACATTAAATCCTGATCTAACTTATGTTATGTTACGTATTATTCGTAAATCTGAAATTACTGGTATGTCACAAGCATTACAGGATCTCTCTGTGTCTTTACCAACAACGGAAATCAGGATGTTTTGCACTGTAATGCAGCAAAGTCTGAATTTTGGTTCCTCTATTTATCATCAATTAATTCAACTATCGACTGATATTAGAGAAATTCAATTATTAGCTATAGAAGAAAAGCTTGGTACGCTGTCAGCAAAAATGAGTATCCCTTTGATTCTATTTATTATGTTTCCAATTATTATTTTAATTCTAGCTCCAGGCGTAATGAGGGTATTTCCACA  >  1/1485660‑1486072                                                                                                                                                                                          |                                                                                                                                                                                                                                    aTTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTAtcatca                                                                                                                                                                    >  1:699177/1‑251 (MQ=255)     aTTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCaacta                                                                                                                                                                >  1:510932/1‑248 (MQ=255)                             cgggaatgggTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCaactagttc                                                                                                                                                            >  1:158094/10‑224 (MQ=255)                                      gTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGCTTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTaa                                                                                                                                                                                                            >  1:526587/1‑174 (MQ=255)                                      gTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTgtac                                                                                                                                                                                                                 <  1:842277/169‑4 (MQ=255)                                                 gcgcTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCtat                                                                                                                    >  1:723457/4‑251 (MQ=255)                                                 gcgcTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCtat                                                                                                                    >  1:49593/4‑251 (MQ=255)                                                 gcgcTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCtat                                                                                                                    >  1:238065/4‑251 (MQ=255)                                                              ttGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTaa                                                                                                                                                                                                            >  1:597789/1‑150 (MQ=255)                                                                        cTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGt                                                                                                    >  1:566096/1‑244 (MQ=255)                                                                               ataTTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCctc                                                                                                                                                                                <  1:690597/159‑1 (MQ=255)                                                                               ataTTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACtt                                                                                                       >  1:717775/3‑234 (MQ=255)                                                                               ataTTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTAcac                                                                                                >  1:748897/3‑239 (MQ=255)                                                                               ataTTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATATCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTAc                                                                                                  >  1:344678/3‑239 (MQ=255)                                                                                 aTTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTAc                                                                                                  <  1:644185/237‑1 (MQ=255)                                                                                      acccTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTa                                                                                                                                                       >  1:599117/3‑179 (MQ=255)                                                                                       cccTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTagag                                                                                                                                       >  1:657193/2‑194 (MQ=255)                                                                                           gATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGAc                                                                                                                                                  <  1:474858/179‑1 (MQ=255)                                                                                           gATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTAcac                                                                                                >  1:575591/1‑227 (MQ=255)                                                                                                gaCTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTatcat                                                                                                                                                                      >  1:664522/2‑154 (MQ=255)                                                                                                gaCTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTat                                                                                                                                                                         <  1:176079/150‑1 (MQ=255)                                                                                                gaCTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTg                                                                      <  1:700864/249‑1 (MQ=255)                                                                                                 aCTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGAt                                                                    <  1:371500/251‑1 (MQ=255)                                                                                                  cTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATc                                                                                                                                                                                                                               <  1:650303/95‑1 (MQ=255)                                                                                                     atgttatgttACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAat                                                                                                                              >  1:276567/1‑189 (MQ=255)                                                                                                     atgttatgttACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATagaag                                                                                                               <  1:92998/204‑1 (MQ=255)                                                                                                     atgttatgttACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTAcac                                                                                                >  1:483186/1‑217 (MQ=255)                                                                                                     atgttatgttACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGt                                                                                             <  1:33746/222‑1 (MQ=255)                                                                                                     atgttatgttACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCta                                                                >  1:206450/1‑251 (MQ=255)                                                                                                     atgttatgttACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCta                                                                >  1:372986/1‑251 (MQ=255)                                                                                                            gttaCGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGaaaaac                                                                                                         >  1:577912/1‑201 (MQ=255)                                                                                                              taCGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCTATTTATTATg                                                       <  1:320575/251‑1 (MQ=255)                                                                                                               aCGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCTATttatta                                                         <  1:592315/248‑1 (MQ=255)                                                                                                                   attattCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCTATTTATTATGTTTc                                                   >  1:652373/1‑250 (MQ=255)                                                                                                                        tCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGtgt                                                                             >  1:599371/1‑217 (MQ=255)                                                                                                                                       taccggTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTg                                                                      <  1:212729/207‑1 (MQ=255)                                                                                                                                         ccggTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTgtac                                                                                                                                                                                                                 <  1:144597/68‑4 (MQ=255)                                                                                                                                         ccggTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCtattt                                                             <  1:881872/216‑1 (MQ=255)                                                                                                                                            gTATGTCGCAGGCATTGCAGGATCTCTCTGTGTC‑TTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGAt                                                                    <  1:201559/207‑1 (MQ=255)                                                                                                                                                gtcgcaggCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGAtt                                                                   <  1:31248/198‑1 (MQ=255)                                                                                                                                                gtcgcaggCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCTATTtattat                                                        <  1:503833/209‑1 (MQ=255)                                                                                                                                                gtcgcaggCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCTATTTATTATGTTTCCAATTATTATTTTAATTCTGGCTCCAggtg                     >  1:504702/8‑249 (MQ=255)                                                                                                                                                gtcgcaggCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCTATTTATTATGTTTCCAATTATTATTTTAATTCTGGCTCCAggtg                     >  1:525042/8‑249 (MQ=255)                                                                                                                                                       gCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCTATTTATTATGTTTCCAATTATTATTTTAATTCTGGCTCCAGGTGTAATg                <  1:601724/249‑1 (MQ=255)                                                                                                                                                       gCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCTATTTATTATGTTTCCAATTATTATTTTAATTCTGGCTCCAGGTGTAATg                <  1:703287/249‑1 (MQ=255)                                                                                                                                                       gCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCTATTTATTATGTTTCCAATTATTATTTTAATTCTGGCTCCAGGTGTAATGAg              >  1:6919/1‑251 (MQ=255)                                                                                                                                                            gcAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCTATTTATTATGTTTCCAATTATTATTTTAATTCTGGCTCCAGGTGTAATGAGGGTAt         >  1:131174/2‑251 (MQ=255)                                                                                                                                                                gATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCTATTTATTATGTTTCCAATTATTATTTTAATTCTGGCTCCAGGTGTAATGAGGGTATTTc      <  1:800543/250‑1 (MQ=255)                                                                                                                                                                 aTCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCTATTTATTATGTTTCCAATTATTATTTTAATTCTGGCTCCAGGTGTAATGAGGGTATTTCca    >  1:530449/1‑251 (MQ=255)                                                                                                                                                                   ctctctGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGaaaaac                                                                                                         >  1:718742/1‑146 (MQ=255)                                                                                                                                                                   ctctctGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATg                                                                                  >  1:149011/1‑171 (MQ=255)                                                                                                                                                                   ctctctGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCTATTTATTATGTTTCCAATTATTATTTTAATTCTGGCTCCAGGTGTAATGAGGGTATTTCcaca  >  1:821149/1‑251 (MQ=255)                                                                                                                                                                     ctctGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCTATTTATTATGTTTCCAATTATTATTTTAATTCTGGCTCCAGGTGTAATGa               >  1:75071/1‑236 (MQ=255)                                                                                                                                                                                          |                                                                                                                                                                                                                                    ATTGATTTAGTAGCTGTTAATGTACAAACAGGGATAAGTATTGAGGCCGCACTAAAACAGGTTGCAACTGACTTTAAAACATTAAATCCTGATCTAACTTATGTTATGTTACGTATTATTCGTAAATCTGAAATTACTGGTATGTCACAAGCATTACAGGATCTCTCTGTGTCTTTACCAACAACGGAAATCAGGATGTTTTGCACTGTAATGCAGCAAAGTCTGAATTTTGGTTCCTCTATTTATCATCAATTAATTCAACTATCGACTGATATTAGAGAAATTCAATTATTAGCTATAGAAGAAAAGCTTGGTACGCTGTCAGCAAAAATGAGTATCCCTTTGATTCTATTTATTATGTTTCCAATTATTATTTTAATTCTAGCTCCAGGCGTAATGAGGGTATTTCCACA  >  1/1485660‑1486072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑