Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,485,788	T→C	S191S (TCT→TCC)	unknown →	Type II/IV secretion system protein TadC, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,485,788	0	T	C	100.0%	129.7 / NA	46	S191S (TCT→TCC)	unknown	Type II/IV secretion system protein TadC, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/15);  total (31/15)

ATATTAATAATAACTATTGTCATTCCTGGGGTTTTAATTGGTTCTATTTTGAAAGCTAAAATTAAAAAAATCATGAATGATTTAGCCGGATTCATTGATTTAGTAGCTGTTAATGTACAAACAGGGATAAGTATTGAGGCCGCACTAAAACAGGTTGCAACTGACTTTAAAACATTAAATCCTGATCTAACTTATGTTATGTTACGTATTATTCGTAAATCTGAAATTACTGGTATGTCACAAGCATTACAGGATCTCTCTGTGTCTTTACCAACAACGGAAATCAGGATGTTTTGCACTGTAATGCAGCAAAGTCTGAATTTTGGTTCCTCTATTTATCATCAATTAATTCAACTATCGACTGATATTAGAGAAATTCAATTATTAGCTATAGAAGAAAAGCTTGGTACGCTGTCAGCAAAAATGAGTATCCCTTTGATTCTATTTATTATGTTTC  >  1/1485567‑1486023                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            atatTAATAGTAACTATTGTTATTCCAGGAATTTTAATTGGTTCTATTTTAAAATCCAAAATTAAAAAAATCATGAATGATTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTAc                                                                                                                                                                                                                                     <  1:70082/230‑1 (MQ=255)  tatTAATAGTAACTATTGTTATTCCAGGAATTTTAATTGGTTCTATTTTAAAATCCAAAATTAAAAAAATCATGAATGATTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGCCTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAg                                                                                                                                                                                                               >  1:612008/1‑251 (MQ=255)      aatagtaaCTATTGTTATTCCAGGAATTTTAATTGGTTCTATTTTAAAATCCAAAATTAAAAAAATCATGAATGATTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGAtc                                                                                                                                                                                                           <  1:44947/251‑1 (MQ=255)           taaCTATTGTTATTCCAGGAATTTTAATTGGTTCTATTTTAAAATCCAAAATTAAAAAAATCATGAATGATTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGAtctctc                                                                                                                                                                                                       >  1:795950/1‑250 (MQ=255)                                              attttaaaatccaaaaTTAAAAAAATCATGAATGATTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTc                                                                                                                                                                                                 >  1:665696/13‑221 (MQ=255)                                                                                aTTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGAtctc                                                                                                                                                                                                         >  1:251250/1‑179 (MQ=255)                                                                                              aTTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTAtcatca                                                                                                                   >  1:699177/1‑251 (MQ=255)                                                                                                  aTTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCaacta                                                                                                               >  1:510932/1‑248 (MQ=255)                                                                                                     tagtagCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTAcc                                                                                                                                                                                           >  1:350690/1‑172 (MQ=255)                                                                                                                          cgggaatgggTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCaactagttc                                                                                                           >  1:158094/10‑224 (MQ=255)                                                                                                                                   gTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGCTTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTaa                                                                                                                                                           >  1:526587/1‑174 (MQ=255)                                                                                                                                   gTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGAtctctc                                                                                                                                                                                                       >  1:449317/1‑130 (MQ=255)                                                                                                                                   gTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTgtac                                                                                                                                                                <  1:842277/169‑4 (MQ=255)                                                                                                                                              gcgcTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCtat                                                                   >  1:49593/4‑251 (MQ=255)                                                                                                                                              gcgcTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCtat                                                                   >  1:723457/4‑251 (MQ=255)                                                                                                                                              gcgcTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCtat                                                                   >  1:238065/4‑251 (MQ=255)                                                                                                                                                           ttGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTaa                                                                                                                                                           >  1:597789/1‑150 (MQ=255)                                                                                                                                                                     cTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGt                                                   >  1:566096/1‑244 (MQ=255)                                                                                                                                                                            ataTTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTc                                                                                                                                                                                                 >  1:795142/3‑95 (MQ=255)                                                                                                                                                                            ataTTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCctc                                                                                                                               <  1:690597/159‑1 (MQ=255)                                                                                                                                                                            ataTTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACtt                                                      >  1:717775/3‑234 (MQ=255)                                                                                                                                                                            ataTTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTAcac                                               >  1:748897/3‑239 (MQ=255)                                                                                                                                                                            ataTTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATATCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTAc                                                 >  1:344678/3‑239 (MQ=255)                                                                                                                                                                             taTTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTc                                                                                                                                                                                                 <  1:272310/93‑1 (MQ=255)                                                                                                                                                                              aTTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTAc                                                 <  1:644185/237‑1 (MQ=255)                                                                                                                                                                                   acccTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTa                                                                                                      >  1:599117/3‑179 (MQ=255)                                                                                                                                                                                    cccTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTagag                                                                                      >  1:657193/2‑194 (MQ=255)                                                                                                                                                                                        gATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGAc                                                                                                 <  1:474858/179‑1 (MQ=255)                                                                                                                                                                                        gATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTAcac                                               >  1:575591/1‑227 (MQ=255)                                                                                                                                                                                             gaCTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTatcat                                                                                                                     >  1:664522/2‑154 (MQ=255)                                                                                                                                                                                             gaCTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTat                                                                                                                        <  1:176079/150‑1 (MQ=255)                                                                                                                                                                                             gaCTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTg                     <  1:700864/249‑1 (MQ=255)                                                                                                                                                                                              aCTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGAt                   <  1:371500/251‑1 (MQ=255)                                                                                                                                                                                               cTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATc                                                                                                                                                                              <  1:650303/95‑1 (MQ=255)                                                                                                                                                                                                  atgttatgttACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTAcc                                                                                                                                                                                           >  1:436169/1‑79 (MQ=255)                                                                                                                                                                                                  atgttatgttACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAat                                                                             >  1:276567/1‑189 (MQ=255)                                                                                                                                                                                                  atgttatgttACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATagaag                                                              <  1:92998/204‑1 (MQ=255)                                                                                                                                                                                                  atgttatgttACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTAcac                                               >  1:483186/1‑217 (MQ=255)                                                                                                                                                                                                  atgttatgttACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGt                                            <  1:33746/222‑1 (MQ=255)                                                                                                                                                                                                  atgttatgttACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCta               >  1:372986/1‑251 (MQ=255)                                                                                                                                                                                                  atgttatgttACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCta               >  1:206450/1‑251 (MQ=255)                                                                                                                                                                                                         gttaCGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGaaaaac                                                        >  1:577912/1‑201 (MQ=255)                                                                                                                                                                                                           taCGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCTATTTATTATg      <  1:320575/251‑1 (MQ=255)                                                                                                                                                                                                            aCGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCTATttatta        <  1:592315/248‑1 (MQ=255)                                                                                                                                                                                                                attattCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGTGTCCCTTTGATTCTATTTATTATGTTTc  >  1:652373/1‑250 (MQ=255)                                                                                                                                                                                                                     tCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTACACTGTCGGCAAAAATGAGtgt                            >  1:599371/1‑217 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            ATATTAATAATAACTATTGTCATTCCTGGGGTTTTAATTGGTTCTATTTTGAAAGCTAAAATTAAAAAAATCATGAATGATTTAGCCGGATTCATTGATTTAGTAGCTGTTAATGTACAAACAGGGATAAGTATTGAGGCCGCACTAAAACAGGTTGCAACTGACTTTAAAACATTAAATCCTGATCTAACTTATGTTATGTTACGTATTATTCGTAAATCTGAAATTACTGGTATGTCACAAGCATTACAGGATCTCTCTGTGTCTTTACCAACAACGGAAATCAGGATGTTTTGCACTGTAATGCAGCAAAGTCTGAATTTTGGTTCCTCTATTTATCATCAATTAATTCAACTATCGACTGATATTAGAGAAATTCAATTATTAGCTATAGAAGAAAAGCTTGGTACGCTGTCAGCAAAAATGAGTATCCCTTTGATTCTATTTATTATGTTTC  >  1/1485567‑1486023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑