Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,485,755	A→G	L180L (CTA→CTG)	unknown →	Type II/IV secretion system protein TadC, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,485,755	0	A	G	100.0%	112.9 / NA	40	L180L (CTA→CTG)	unknown	Type II/IV secretion system protein TadC, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/11);  total (29/11)

TTTACTGATGAAACTACCGACAGTTCATCTCTGTTACTTGGCTTTCTAACTATATTAATAATAACTATTGTCATTCCTGGGGTTTTAATTGGTTCTATTTTGAAAGCTAAAATTAAAAAAATCATGAATGATTTAGCCGGATTCATTGATTTAGTAGCTGTTAATGTACAAACAGGGATAAGTATTGAGGCCGCACTAAAACAGGTTGCAACTGACTTTAAAACATTAAATCCTGATCTAACTTATGTTATGTTACGTATTATTCGTAAATCTGAAATTACTGGTATGTCACAAGCATTACAGGATCTCTCTGTGTCTTTACCAACAACGGAAATCAGGATGTTTTGCACTGTAATGCAGCAAAGTCTGAATTTTGGTTCCTCTATTTATCATCAATTAATTCAACTATCGACTGATATTAGAGAAATTCAATTATTAGCTATAGAAGAAAAGCTTGGTACGC  >  1/1485516‑1485978                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                cttattgataaAACTACTGACAGCTCATCTCTGTTACTTGGCTTTCTAACTATATTAATAGTAACTATTGTTATTCCAGGAATTTTAATTGGTTCTATTTTAAAATCCAAAATTAAAAAAATCATGAATGATTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACttatgttat                                                                                                                                                                                                                      >  1:527838/6‑251 (MQ=255)                actgACAGCTCATCTCTGTTACTTGGCTTTCTAACTATATTAATAGTAACTATTGTTATTCCAGGAATTTTAATTGGTTCTATTTTAAAATCCAAAATTAAAAAAATCATGAATGATTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGt                                                                                                                                                                                                               <  1:113262/240‑1 (MQ=255)                actgACAGCTCATCTCTGTTACTTGGCTTTCTAACTATATTAATAGTAACTATTGTTATTCCAGGAATTTTAATTGGTTCTATTTTAAAATCCAAAATTAAAAAAATCATGAATGATTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCg                                                                                                                                                                                                       >  1:205908/4‑251 (MQ=255)                         tCATCTCTGTTACTTGGCTTTCTAACTATATTAATAGTAACTATTGTTATTCCAGGAATTTTAATTGGTTCTATTTTAACATCCAAAATTAAAAAAATCATGAATGATTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATccga                                                                                                                                                                                              >  1:694927/1‑248 (MQ=255)                                                    atatTAATAGTAACTATTGTTATTCCAGGAATTTTAATTGGTTCTATTTTAAAATCCAAAATTAAAAAAATCATGAATGATTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTtatgttatgt                                                                                                                                                                                                                    >  1:578923/1‑202 (MQ=255)                                                    atatTAATAGTAACTATTGTTATTCCAGGAATTTTAATTGGTTCTATTTTAAAATCCAAAATTAAAAAAATCATGAATGATTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTtatgttatgt                                                                                                                                                                                                                    <  1:254665/202‑1 (MQ=255)                                                    atatTAATAGTAACTATTGTTATTCCAGGAATTTTAATTGGTTCTATTTTAAAATCCAAAATTAAAAAAATCATGAATGATTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTAc                                                                                                                                                                                        <  1:70082/230‑1 (MQ=255)                                                     tatTAATAGTAACTATTGTTATTCCAGGAATTTTAATTGGTTCTATTTTAAAATCCAAAATTAAAAAAATCATGAATGATTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGCCTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAg                                                                                                                                                                  >  1:612008/1‑251 (MQ=255)                                                     tatTAATAGTAACTATTGTTATTCCAGGAATTTTAATTGGTTCTATTTTAAAATCCAAAATTAAAAAAATCATGAATGATTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACttatgt                                                                                                                                                                                                                         <  1:341250/196‑1 (MQ=255)                                                         aatagtaaCTATTGTTATTCCAGGAATTTTAATTGGTTCTATTTTAAAATCCAAAATTAAAAAAATCATGAATGATTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGtat                                                                                                                                                                                                             >  1:662688/1‑204 (MQ=255)                                                         aatagtaaCTATTGTTATTCCAGGAATTTTAATTGGTTCTATTTTAAAATCCAAAATTAAAAAAATCATGAATGATTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGAtc                                                                                                                                                              <  1:44947/251‑1 (MQ=255)                                                              taaCTATTGTTATTCCAGGAATTTTAATTGGTTCTATTTTAAAATCCAAAATTAAAAAAATCATGAATGATTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGAtctctc                                                                                                                                                          >  1:795950/1‑250 (MQ=255)                                                                 cTATTGTTATTCCAGGAATTTTAATTGGTTCTATTTTAAAATCCAAAATTAAAAAAATCATGAATGATTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACttatgtta                                                                                                                                                                                                                       >  1:320163/1‑186 (MQ=255)                                                                                                 attttaaaatccaaaaTTAAAAAAATCATGAATGATTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTc                                                                                                                                                    >  1:665696/13‑221 (MQ=255)                                                                                                                                   aTTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGAtctc                                                                                                                                                            >  1:251250/1‑179 (MQ=255)                                                                                                                                                 aTTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTAtcatca                                                                      >  1:699177/1‑251 (MQ=255)                                                                                                                                                     aTTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCaacta                                                                  >  1:510932/1‑248 (MQ=255)                                                                                                                                                        tagtagCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTAcc                                                                                                                                              >  1:350690/1‑172 (MQ=255)                                                                                                                                                                             cgggaatgggTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCaactagttc                                                              >  1:158094/10‑224 (MQ=255)                                                                                                                                                                                      gTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATccgaga                                                                                                                                                                                            <  1:276709/96‑6 (MQ=255)                                                                                                                                                                                      gTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGCTTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTaa                                                                                                              >  1:526587/1‑174 (MQ=255)                                                                                                                                                                                      gTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGAtctctc                                                                                                                                                          >  1:449317/1‑130 (MQ=255)                                                                                                                                                                                      gTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTgtac                                                                                                                   <  1:842277/169‑4 (MQ=255)                                                                                                                                                                                             agctgcgcTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTa                                                                                                                                                                                                     >  1:271890/8‑80 (MQ=255)                                                                                                                                                                                                 gcgcTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCtat                      >  1:238065/4‑251 (MQ=255)                                                                                                                                                                                                 gcgcTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCtat                      >  1:49593/4‑251 (MQ=255)                                                                                                                                                                                                 gcgcTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCtat                      >  1:723457/4‑251 (MQ=255)                                                                                                                                                                                                              ttGCAACTGACTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTaa                                                                                                              >  1:597789/1‑150 (MQ=255)                                                                                                                                                                                                                        cTTTAAAATATTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGt      >  1:566096/1‑244 (MQ=255)                                                                                                                                                                                                                               ataTTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTc                                                                                                                                                    >  1:795142/3‑95 (MQ=255)                                                                                                                                                                                                                               ataTTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCctc                                                                                  <  1:690597/159‑1 (MQ=255)                                                                                                                                                                                                                               ataTTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACtt         >  1:717775/3‑234 (MQ=255)                                                                                                                                                                                                                               ataTTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTAcac  >  1:748897/3‑239 (MQ=255)                                                                                                                                                                                                                               ataTTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATATCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTAc    >  1:344678/3‑239 (MQ=255)                                                                                                                                                                                                                                taTTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTc                                                                                                                                                    <  1:272310/93‑1 (MQ=255)                                                                                                                                                                                                                                 aTTAAACCCTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTAc    <  1:644185/237‑1 (MQ=255)                                                                                                                                                                                                                                      acccTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTa                                                         >  1:599117/3‑179 (MQ=255)                                                                                                                                                                                                                                       cccTGATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTagag                                         >  1:657193/2‑194 (MQ=255)                                                                                                                                                                                                                                           gATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGAc                                                    <  1:474858/179‑1 (MQ=255)                                                                                                                                                                                                                                           gATCTGACTTATGTTATGTTACGTATTATTCGTAAATCCGAGATTACCGGTATGTCGCAGGCATTGCAGGATCTCTCTGTGTCTTTACCAACAGCAGAAATCCGAATGTTTTGTACTGTAATGCAACAAAGCCTTAATTTTGGTTCCTCTATTTATCATCAACTAGTTCAGCTATCGACTGATATTAGAGAAATTCAATTATTGGCTATAGAAGAAAAACTTGGTAcac  >  1:575591/1‑227 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                TTTACTGATGAAACTACCGACAGTTCATCTCTGTTACTTGGCTTTCTAACTATATTAATAATAACTATTGTCATTCCTGGGGTTTTAATTGGTTCTATTTTGAAAGCTAAAATTAAAAAAATCATGAATGATTTAGCCGGATTCATTGATTTAGTAGCTGTTAATGTACAAACAGGGATAAGTATTGAGGCCGCACTAAAACAGGTTGCAACTGACTTTAAAACATTAAATCCTGATCTAACTTATGTTATGTTACGTATTATTCGTAAATCTGAAATTACTGGTATGTCACAAGCATTACAGGATCTCTCTGTGTCTTTACCAACAACGGAAATCAGGATGTTTTGCACTGTAATGCAGCAAAGTCTGAATTTTGGTTCCTCTATTTATCATCAATTAATTCAACTATCGACTGATATTAGAGAAATTCAATTATTAGCTATAGAAGAAAAGCTTGGTACGC  >  1/1485516‑1485978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑