Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	272,984	T→C	T228A (ACC→GCC)	unknown ←	Isoleucyl‑tRNA synthetase (EC 6.1.1.5)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	272,984	0	T	C	100.0%	201.1 / NA	73	T228A (ACC→GCC)	unknown	Isoleucyl‑tRNA synthetase (EC 6.1.1.5)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/39);  total (34/39)

GAATAACTCAAGATCTTTACCTTTTGCGGAACCTAAGATTTCCACCTTTTCCACGCCAACCGCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGTGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCACTTTGCCCAAAGTGCGAATAATGTTGGCTTCGGTGTTGAAATTCATGGTGAGGTACGGATTATCCCAATCGCCCAACAC  >  1/272751‑273230                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       gAATAACTCAAGATCTTTACCTTTTGCGGAACCTAAGATTTCCACCTTTTCCACGCCAACCGCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCTCTGCCTTTGCCGACGGCGTTAAATTTACCTTCaa                                                                                                                                                                                                                                       >  1:849928/1‑251 (MQ=255) gAATAACTCAAGATCTTTACCTTTTGCGGAACCTAAGATTTCCACCTTTTCCACGCCAACCGCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCaa                                                                                                                                                                                                                                       >  1:260179/1‑251 (MQ=255) gAATAACTCAAGATCTTTACCTTTTGCGGAACCTAAGATTTCCACCTTTTCCACGCCAACCGCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCaa                                                                                                                                                                                                                                       >  1:773453/1‑251 (MQ=255)      aCTCAAGATCTTTACCTTTTGCGGAACCTAAGATTTCCACCTTTTCCACGCCAACCGCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAAt                                                                                                                                                                                                                                                 <  1:880692/236‑1 (MQ=255)       cTCAAGATCTTTACCTTTTGCGGAACCTAAGATTTCCACCTTTTCCACGCCAACCGCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCa                                                                                                                                                                                                                                 >  1:309261/1‑251 (MQ=255)       cTCAAGATCTTTACCTTTTGCGGAACCTAAGATTTCCACCTTTTCCACGCCAACCGCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCa                                                                                                                                                                                                                                 >  1:593741/1‑251 (MQ=255)        tCAAGATCTTTACCTTTTGCGGAACCTAAGATTTCCACCTTTTCCACGCCAACCGCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCAc                                                                                                                                                                                                                                <  1:770876/251‑1 (MQ=255)              tCTTTACCTTTTGCGGAACCTAAGATTTCCACCTTTTCCACGCCAACCGCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCa                                                                                                                                                                                                                            >  1:523778/1‑249 (MQ=255)              tCTTTACCTTTTGCGGAACCTAAGATTTCCACCTTTTCCACGCCAACCGCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCa                                                                                                                                                                                                                            >  1:791989/1‑249 (MQ=255)              tCTTTACCTTTTGCGGAACCTAAGATTTCCACCTTTTCCACGCCAACCGCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAACTTCCACCGCa                                                                                                                                                                                                                            >  1:51361/1‑249 (MQ=255)                               aCCTAAGATTTCCACCTTTTCCACGCCAACCGCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTa                                                                                                                                                                                                         >  1:275073/1‑251 (MQ=255)                               aCCTAAGATTTCCACCTTTTCCACGCCAACCGCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTa                                                                                                                                                                                                         >  1:517982/1‑251 (MQ=255)                                ccTAAGATTTCCACCTTTTCCACGCCAACCGCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTAc                                                                                                                                                                                                        <  1:848451/251‑1 (MQ=255)                                      aTTTCCACCTTTTCCACGCCAACCGCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATaaa                                                                                                                                                                                                   <  1:130051/250‑1 (MQ=255)                                        ttCCACCTTTTCCACGCCAACCGCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATgg                                                                                                                                                                                                <  1:627530/251‑1 (MQ=255)                                                tttCCACGCCAACCGCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGa                                                                                                                                                                                         >  1:323629/1‑250 (MQ=255)                                                   ccACGCCAACCGCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGacaca                                                                                                                                                                                     >  1:129074/1‑251 (MQ=255)                                                    cACGCCAACCGCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATACTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGacacac                                                                                                                                                                                    <  1:452042/251‑1 (MQ=255)                                                        ccAACCGCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTc                                                                                                                                                                                <  1:71694/251‑1 (MQ=255)                                                              gCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATaaa                                                                                                                                                                                                   >  1:329374/1‑226 (MQ=255)                                                               ccTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCatat                                                                                                                                                                         <  1:311918/251‑1 (MQ=255)                                                                 ttCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTc                                                                                                                                                                       <  1:294542/251‑1 (MQ=255)                                                                  tCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCata                                                                                                                                                                          <  1:282257/247‑1 (MQ=255)                                                                  tCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTc                                                                                                                                                                       >  1:85354/1‑250 (MQ=255)                                                                    aCCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGAc                                                                                                                                                                                              >  1:690470/1‑225 (MQ=255)                                                                    aCCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCAc                                                                                                                                                                    <  1:758852/251‑1 (MQ=255)                                                                       aTGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTTTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCActtc                                                                                                                                                                 <  1:658156/251‑1 (MQ=255)                                                                         gTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGacacac                                                                                                                                                                                    <  1:720548/230‑1 (MQ=255)                                                                           cTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCActtccg                                                                                                                                                               >  1:515649/1‑249 (MQ=255)                                                                                            gCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATaaa                                                                                                                                                                                                   >  1:580458/1‑196 (MQ=255)                                                                                                     ccgcgCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCaa                                                                                                                                   <  1:233864/250‑1 (MQ=255)                                                                                                          cTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACGTaaa                                                                                                                                                                                                   >  1:480495/1‑182 (MQ=255)                                                                                                          cTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCata                                                                                                                                                                          <  1:20944/207‑1 (MQ=255)                                                                                                          cTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAacac                                                                                                                               >  1:102308/1‑250 (MQ=255)                                                                                                          cTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAacac                                                                                                                               >  1:14693/1‑250 (MQ=255)                                                                                                              tcgCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGACACAATCCAAACACCAATg                                                                                                                          >  1:335165/1‑251 (MQ=255)                                                                                                                gCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCatat                                                                                                                                                                         <  1:103592/202‑1 (MQ=255)                                                                                                                 ccTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCAc                                                                                                                       <  1:326956/251‑1 (MQ=255)                                                                                                                    aaTTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCgg                                                                                                                    <  1:676541/251‑1 (MQ=255)                                                                                                                        gCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACttt                                                                                                                                                                                                                        <  1:322673/147‑1 (MQ=255)                                                                                                                               cTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATaaa                                                                                                                                                                                                   >  1:899824/1‑161 (MQ=255)                                                                                                                               cTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCatat                                                                                                                                                                         >  1:578149/1‑187 (MQ=255)                                                                                                                                tGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCttt                                                                                                        <  1:425018/251‑1 (MQ=255)                                                                                                                                                      cGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTcc                                                                                                                                                                      <  1:641290/167‑1 (MQ=255)                                                                                                                                                      cGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTtata                                                                                                     >  1:406469/1‑232 (MQ=255)                                                                                                                                                      cGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGAt                                                                                   >  1:671084/1‑250 (MQ=255)                                                                                                                                                        gAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCa                                                                                 >  1:364227/1‑250 (MQ=255)                                                                                                                                                         aaaTAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCAc                                                                                <  1:872232/250‑1 (MQ=255)                                                                                                                                                              gCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCata                                                                                                                                                                          <  1:307834/155‑1 (MQ=255)                                                                                                                                                                 cGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCACTTTGCCCaa                                                                       >  1:653013/1‑251 (MQ=255)                                                                                                                                                                   gTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGt                                                                                                                                                                                                          <  1:160346/118‑1 (MQ=255)                                                                                                                                                                           gCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCACTTTGCCCAAAGTGCGaata                                                             >  1:421152/1‑251 (MQ=255)                                                                                                                                                                               ggTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCACTTTGCCCAAAGTg                                                                   <  1:484540/241‑1 (MQ=255)                                                                                                                                                                                gTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCACTTTGCCCAAAGTGCGaataa                                                            <  1:810888/247‑1 (MQ=255)                                                                                                                                                                                gTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCACTTTGCCCAAAGTGCGAATAATGtt                                                        >  1:811029/1‑251 (MQ=255)                                                                                                                                                                                gTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCACTTTGCCCAAAGTGCGAATAATGtt                                                        >  1:695500/1‑251 (MQ=255)                                                                                                                                                                                         gTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCACTTTGCCCAAAGTGCGAATAATGTTGGCTTCgg                                                <  1:500478/250‑1 (MQ=255)                                                                                                                                                                                            gcgccgaatcacggtttggACGACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCgg                                                                                                                    >  1:52571/18‑179 (MQ=255)                                                                                                                                                                                               gTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTtatat                                                                                                    >  1:205470/1‑192 (MQ=255)                                                                                                                                                                                                tCCAAATCACGGCTGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCACTTTGCCCAAAGTGCGAATAATGTTGGCTTCGGTGTTGaaa                                        <  1:82745/251‑1 (MQ=255)                                                                                                                                                                                                     aTCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCACTTTGCCCAAAGTGCGAATAATGTTGGCTTCGGTGTTGAAATTCAt                                   <  1:605790/251‑1 (MQ=255)                                                                                                                                                                                                               aCAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCACTTTGCCCAAAGTGCGAATAATGTTGGCTTCGGTGTTGAAATTCATGGTGAGGTAc                         <  1:206707/251‑1 (MQ=255)                                                                                                                                                                                                                cAACTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTtatata                                                                                                   <  1:519773/176‑1 (MQ=255)                                                                                                                                                                                                                   cTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCACTTTGCCCAAAGTGCGAATAATGTTGGCTTCGGTGTTGAAATTCATGGTGAGGTACGGAt                     <  1:148897/251‑1 (MQ=255)                                                                                                                                                                                                                   cTTGCCGCTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCACTTTGCCCAAAGTGCGAACAATGTTGGCTTCGGTGTTGAAATTCATGGTGAGGTACGGAt                     <  1:111423/251‑1 (MQ=255)                                                                                                                                                                                                                          cTGCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCACTTTGCCCAAAGTGCGAATAATGTTGGCTTCGGTGTTGAAATTCATGGTGAGGTACGGATTATccc               <  1:582110/250‑1 (MQ=255)                                                                                                                                                                                                                            gCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTtatat                                                                                                    <  1:451008/163‑1 (MQ=255)                                                                                                                                                                                                                            gCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCAc                                                                                <  1:134332/183‑1 (MQ=255)                                                                                                                                                                                                                            gCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCACTTTGCCCAAAGTGCGAATAATGTTGGCTTCGGTGTTGAAATTCATGGTGAGGTACGGAt                     >  1:216653/1‑242 (MQ=255)                                                                                                                                                                                                                            gCCTTTGCCGACGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCACTTTGCCCAAAGTGCGAATAATGTTGGCTTCGGTGTTGAAATTCATGGTGAGGTACGGATTATCCCAAt            >  1:462045/1‑251 (MQ=255)                                                                                                                                                                                                                                      aCGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCACTTTGCCCAAAGTGCGAATAATGTTGGCTTCGGTGTTGAAATTCATGGTGAGGTACGGATTATCCCAATCGCCCAacac  <  1:359975/251‑1 (MQ=255)                                                                                                                                                                                                                                      aCGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCACTTTGCCCAAAGTGCGAATAATGTTGGCTTCGGTGTTGAAATTCATGGTGAGGTACGGATTATCCCAATCGCCCAacac  <  1:813384/251‑1 (MQ=255)                                                                                                                                                                                                                                      aCGGCGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCACTTTGCCCAAAGTGCGAATAATGTTGGCTTCGGTGTTGAAATTCATGGTGAGGTACGGATTATCCCAATCGCCCAacac  <  1:848370/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       GAATAACTCAAGATCTTTACCTTTTGCGGAACCTAAGATTTCCACCTTTTCCACGCCAACCGCCTTCACCATGTCTTCCACCAAATCGGCAGCTAAAATCACGCGCTCGTCGCCTAATTGCACAAGCTGATATTCCAGCTCGGCATTCACGGAAATAGCACGGTTGGACGGCATGGTCCAAGGGGTTGTGGTCCAAATCACGGCGGACAACTTGCCGCTGCCTTTGCCGACGGTGTTAAATTTACCTTCAATTTCCACCGCACTTTCTGCCGGGAAACGTACATAAATGGACGGCGACACACGGTCTTCATATTCCACTTCCGCTTCCGCCAAAGAGGAGCCACAATCCAAACACCAATGCACCGGTTTGGAGCCTTTATATAAATGCCCGTTGGCGATCACTTTGCCCAAAGTGCGAATAATGTTGGCTTCGGTGTTGAAATTCATGGTGAGGTACGGATTATCCCAATCGCCCAACAC  >  1/272751‑273230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑