Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,485,516	T→C	F101L (TTT→CTT)	unknown →	Type II/IV secretion system protein TadC, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,485,516	0	T	C	100.0%	11.5 / NA	6	F101L (TTT→CTT)	unknown	Type II/IV secretion system protein TadC, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/4);  total (2/4)

TGTTTGCCGCACTTTCAGCCAGAAAAAAATTCCAATATAGAAAGGAAGTTATTACTGGAGAAAGACCTAAGGATCAAGATAGTGACGAAGTAGCTAAAAATAAGAGCAAACAGCAAATTGAACTTGAACTATTACTAATTAATAATAATCCTATCTTAAAAGTACTCGGAATTATTGATAAAAATATAAAAGTAAAATTGTTGATCATGCTTTTGCTTTTTGGAGTTTACTATCTTTATTCATTTACTGATGAAACTACCGACAGTTCATCTCTGTTACTTGGCTTTCTAACTATATTAATAATAACTATTGTCATTCCTGGGGTTTTAATTGGTTCTATTTTGAAAGCTAAAATTAAAAAAATCATGAATGATTTAGCCGGATTCATTGATTTAGTAGCTGTTAATGTACAAACAGGGATAAGTATTGAGGCCGCACTAAAACAGGTTGCAACTGACTTTAAAACATTAAATCCTGATCTAA  >  1/1485274‑1485756                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 tatttttggCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAATTACTGATTATGGTTTTACTTTGCGGGGTTTATTATCTTTATTCACTTATtgat                                                                                                                                                                                                                                          <  1:806403/243‑1 (MQ=255)       tggCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAGTGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAATTACTGATTATGGTTTTACTTTGCGGGGTTTATTATCTTTATTCACTTATTGATAAAACt                                                                                                                                                                                                                                    <  1:643794/249‑1 (MQ=255)                                          aaagaaattattaCTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAATTACTGATTATGGTTTTACTTTGCGGGGTTTATTATCTTTATTCACTTATTGATAAAACTACTGACAGCTCATCTCTGTTACTTGGCTTTCTAAc                                                                                                                                                                                                 <  1:282703/244‑1 (MQ=255)                                                          gagaAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAATTACTGATTATGGTTTTACTTTGCGGGGTTTATTATCTTTATTCACTTATTGATAAAACTACTGACAGCTCATCTCTGTTACTTGGCTTTCTAACTATATtaatag                                                                                                                                                                                      <  1:611199/246‑2 (MQ=255)                                                                                                                    aaTTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAATTACTGATTATGGTTTTACTTTGCGGGGTTTATTATCTTTATTCACTTATTGATAAAACTACTGACAGCTCAtctc                                                                                                                                                                                                                    >  1:764065/1‑158 (MQ=255)                                                                                                                                                                                                                                          cTTTATTCACTTATTGATAAAACTACTGACAGCTCATCTCTGTTACTTGGCTTTCTAACTATATTAATAGTAACTATTGTTATTCCAGGAATTTTAATTGGTTCTATTTTAAAATCCAAAATTAAAAAAATCATGAATGATTTAGCAGGTTTCATTGATTTAGTAGCTGTTAATTTACAAACGGGAATGGGTATTGAAGCTGCGCTAAAGCACGTTGCAACTGACTTTAAAATATTAAACCCTGATCtga  >  1:432260/1‑248 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 TGTTTGCCGCACTTTCAGCCAGAAAAAAATTCCAATATAGAAAGGAAGTTATTACTGGAGAAAGACCTAAGGATCAAGATAGTGACGAAGTAGCTAAAAATAAGAGCAAACAGCAAATTGAACTTGAACTATTACTAATTAATAATAATCCTATCTTAAAAGTACTCGGAATTATTGATAAAAATATAAAAGTAAAATTGTTGATCATGCTTTTGCTTTTTGGAGTTTACTATCTTTATTCATTTACTGATGAAACTACCGACAGTTCATCTCTGTTACTTGGCTTTCTAACTATATTAATAATAACTATTGTCATTCCTGGGGTTTTAATTGGTTCTATTTTGAAAGCTAAAATTAAAAAAATCATGAATGATTTAGCCGGATTCATTGATTTAGTAGCTGTTAATGTACAAACAGGGATAAGTATTGAGGCCGCACTAAAACAGGTTGCAACTGACTTTAAAACATTAAATCCTGATCTAA  >  1/1485274‑1485756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑