Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,485,416	T→C	N67N (AAT→AAC)	unknown →	Type II/IV secretion system protein TadC, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,485,416	0	T	C	100.0%	31.3 / NA	13	N67N (AAT→AAC)	unknown	Type II/IV secretion system protein TadC, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/5);  total (8/5)

GGGAATCATTTGGTGGTTGATGAGGAAGTCAACATGATGATTAAACTATTATTATCTTATGCGCTATTAACGATTTTTGGTATTATTGTATTGTTTGCCGCACTTTCAGCCAGAAAAAAATTCCAATATAGAAAGGAAGTTATTACTGGAGAAAGACCTAAGGATCAAGATAGTGACGAAGTAGCTAAAAATAAGAGCAAACAGCAAATTGAACTTGAACTATTACTAATTAATAATAATCCTATCTTAAAAGTACTCGGAATTATTGATAAAAATATAAAAGTAAAATTGTTGATCATGCTTTTGCTTTTTGGAGTTTACTATCTTTATTCATTTACTGATGAAACTACCGACAGTTCATCTCTGTTACTTGGCTTTCTAACTATATTAATAA  >  1/1485183‑1485576                                                                                                                                                                                                                                          |                                                                                                                                                                 gggAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAACTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATcccgttttaaa                                                                                                                                                 >  1:889899/1‑242 (MQ=255)        aTTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATtaacaat                                                                                                                                                               >  1:258979/1‑230 (MQ=255)                                aCATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATcc                                                                                                                                                          >  1:318174/1‑211 (MQ=255)                                            agcTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAAtta                                                                                                         >  1:170225/3‑247 (MQ=255)                                             gcTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATtaacaat                                                                                                                                                               >  1:177660/2‑193 (MQ=255)                                                attactgtCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAAttactgatta                                                                                                  >  1:741339/8‑243 (MQ=255)                                                        cTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAAttactgattatggtttta                                                                                          >  1:761644/1‑235 (MQ=255)                                                         ttatgcattATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAAttactgattatggttttac                                                                                         <  1:347992/243‑18 (MQ=255)                                                                                        gtattatttttggCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAAttac                                                                                                        >  1:738420/13‑203 (MQ=255)                                                                                            tatttttggCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAATTACTGATTATGGTTTTACTTTGCGGGGTTTATTATCTTTATTCACTTATtgat                                                      <  1:806403/243‑1 (MQ=255)                                                                                                  tggCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAGTGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAATTACTGATTATGGTTTTACTTTGCGGGGTTTATTATCTTTATTCACTTATTGATAAAACt                                                <  1:643794/249‑1 (MQ=255)                                                                                                                              gcataggaaagaaattattaCTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTg                                                                                                                                >  1:438670/15‑143 (MQ=255)                                                                                                                                     aaagaaattattaCTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAATTACTGATTATGGTTTTACTTTGCGGGGTTTATTATCTTTATTCACTTATTGATAAAACTACTGACAGCTCATCTCTGTTACTTGGCTTTCTAAc             <  1:282703/244‑1 (MQ=255)                                                                                                                                                     gagaAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAATTACTGATTATGGTTTTACTTTGCGGGGTTTATTATCTTTATTCACTTATTGATAAAACTACTGACAGCTCATCTCTGTTACTTGGCTTTCTAACTATATtaatag  <  1:611199/246‑2 (MQ=255)                                                                                                                                                                                                               aaTTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAATTACTGATTATGGTTTTACTTTGCGGGGTTTATTATCTTTATTCACTTATTGATAAAACTACTGACAGCTCAtctc                                >  1:764065/1‑158 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                 GGGAATCATTTGGTGGTTGATGAGGAAGTCAACATGATGATTAAACTATTATTATCTTATGCGCTATTAACGATTTTTGGTATTATTGTATTGTTTGCCGCACTTTCAGCCAGAAAAAAATTCCAATATAGAAAGGAAGTTATTACTGGAGAAAGACCTAAGGATCAAGATAGTGACGAAGTAGCTAAAAATAAGAGCAAACAGCAAATTGAACTTGAACTATTACTAATTAATAATAATCCTATCTTAAAAGTACTCGGAATTATTGATAAAAATATAAAAGTAAAATTGTTGATCATGCTTTTGCTTTTTGGAGTTTACTATCTTTATTCATTTACTGATGAAACTACCGACAGTTCATCTCTGTTACTTGGCTTTCTAACTATATTAATAA  >  1/1485183‑1485576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑