Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,485,321	G→A	V36I (GTT→ATT)	unknown →	Type II/IV secretion system protein TadC, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,485,321	0	G	A	100.0%	67.0 / NA	26	V36I (GTT→ATT)	unknown	Type II/IV secretion system protein TadC, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/12);  total (14/12)

TTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGCAGTGAACTTTTCGGTATGGGAATCATTTGGTGGTTGATGAGGAAGTCAACATGATGATTAAACTATTATTATCTTATGCGCTATTAACGATTTTTGGTATTATTGTATTGTTTGCCGCACTTTCAGCCAGAAAAAAATTCCAATATAGAAAGGAAGTTATTACTGGAGAAAGACCTAAGGATCAAGATAGTGACGAAGTAGCTAAAAATAAGAGCAAACAGCAAATTGAACTTGAACTATTACTAATTAATAATAATCCTATCTTAAAAGTACTCGGAATTATTGATAAAAATATAAAAGTAAAATTGTTGATCATGCTTTTGCTTTTTGGAGTTTACTATCTTTATTCATTTACTGATGAAACT  >  1/1485091‑1485530                                                                                                                                                                                                                                       |                                                                                                                                                                                                                  tttGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGAc                                                                                                                                                                                                 <  1:279608/249‑1 (MQ=255)  ttGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTa                                                                                                                                                                                              >  1:746596/1‑251 (MQ=255)                 aCTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTAc                                                                                                                                                                                                            <  1:719046/222‑1 (MQ=255)                             taattaatGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCaaagtaatgatgaaatagcca                                                                                                                                                                   <  1:194400/251‑20 (MQ=255)                                           aCCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAACTTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCaaagt                                                                                                                                                                                   >  1:439354/1‑218 (MQ=255)                                               gTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTAc                                                                                                                                                                                                            <  1:163611/192‑1 (MQ=255)                                                        tGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAAttgaac                                                                                                                                        <  1:460298/251‑1 (MQ=255)                                                                   gTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCaaagtaatga                                                                                                                                                                              >  1:791350/1‑194 (MQ=255)                                                                        tggtaGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACtgat                                                                                                                        <  1:343804/247‑4 (MQ=255)                                                                                         gTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTAc                                                                                                                            <  1:877369/230‑1 (MQ=255)                                                                                             gggAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAACTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATcccgttttaaa                                                                                                   >  1:889899/1‑242 (MQ=255)                                                                                               gAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCaaagtaatgatgaaatagcc                                                                                                                                                                    >  1:172888/1‑166 (MQ=255)                                                                                                    aTTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGgag                                                                                                                                                                                                       >  1:637747/1‑144 (MQ=255)                                                                                                    aTTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATtaacaat                                                                                                                 >  1:258979/1‑230 (MQ=255)                                                                                                                            aCATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATcc                                                                                                            >  1:318174/1‑211 (MQ=255)                                                                                                                                        agcTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAAtta                                                           >  1:170225/3‑247 (MQ=255)                                                                                                                                         gcTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATtaacaat                                                                                                                 >  1:177660/2‑193 (MQ=255)                                                                                                                                            attactgtCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAAttactgatta                                                    >  1:741339/8‑243 (MQ=255)                                                                                                                                                  gtCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCaaag                                                                                                                                                                                    >  1:143458/2‑115 (MQ=255)                                                                                                                                                  gtCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACtgat                                                                                                                        <  1:299930/176‑4 (MQ=255)                                                                                                                                                    cTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAAttactgattatggtttta                                            >  1:761644/1‑235 (MQ=255)                                                                                                                                                     ttatgcattATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAAttactgattatggttttac                                           <  1:347992/243‑18 (MQ=255)                                                                                                                                                            ttATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCaaagtaatgatg                                                                                                                                                                            <  1:465881/114‑11 (MQ=255)                                                                                                                                                                                    gtattatttttggCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAAttac                                                          >  1:738420/13‑203 (MQ=255)                                                                                                                                                                                        tatttttggCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAATTACTGATTATGGTTTTACTTTGCGGGGTTTATTATCTTTATTCACTTATtgat        <  1:806403/243‑1 (MQ=255)                                                                                                                                                                                              tggCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAGTGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAATTACTGATTATGGTTTTACTTTGCGGGGTTTATTATCTTTATTCACTTATTGATAAAACt  <  1:643794/249‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                  TTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGCAGTGAACTTTTCGGTATGGGAATCATTTGGTGGTTGATGAGGAAGTCAACATGATGATTAAACTATTATTATCTTATGCGCTATTAACGATTTTTGGTATTATTGTATTGTTTGCCGCACTTTCAGCCAGAAAAAAATTCCAATATAGAAAGGAAGTTATTACTGGAGAAAGACCTAAGGATCAAGATAGTGACGAAGTAGCTAAAAATAAGAGCAAACAGCAAATTGAACTTGAACTATTACTAATTAATAATAATCCTATCTTAAAAGTACTCGGAATTATTGATAAAAATATAAAAGTAAAATTGTTGATCATGCTTTTGCTTTTTGGAGTTTACTATCTTTATTCATTTACTGATGAAACT  >  1/1485091‑1485530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑