Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,485,234	T→C	L7M (TTA→CTG)	unknown →	Type II/IV secretion system protein TadC, associated with Flp pilus assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,485,234	0	T	C	100.0%	121.1 / NA	43	L7M (TTA→CTG)	unknown	Type II/IV secretion system protein TadC, associated with Flp pilus assembly
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/23);  total (20/23)

TAAAATGGAGAAGAAAAAGAAAGCAATGACATCAGAGGCTAGAATGTCAGCTATAATTGTAGCGTGTTTTCCTGTCGGTTTCTTTTTCTTTATGCAATTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGCAGTGAACTTTTCGGTATGGGAATCATTTGGTGGTTGATGAGGAAGTCAACATGATGATTAAACTATTATTATCTTATGCGCTATTAACGATTTTTGGTATTATTGTATTGTTTGCCGCACTTTCAGCCAGAAAAAAATTCCAATATAGAAAGGAAGTTATTACTGGAGAAAGACCTAAGGATCAAGATAGTGACGAAGTAGCTAAAAATAAGAGCAAACAGCAAATTGAACTTGAACTATTACTAATTAATAATAATCCTATCTTAAAAGTACTCGGAATTATTGATAAAAATATAAAAGTAAAATTG  >  1/1484994‑1485473                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                tAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTTTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATgc                                                                                                                                                                                                                                       <  1:424744/251‑1 (MQ=255) tAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATgc                                                                                                                                                                                                                                       <  1:135023/251‑1 (MQ=255) tAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATgc                                                                                                                                                                                                                                       <  1:364426/251‑1 (MQ=255)  aaaaTGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATgca                                                                                                                                                                                                                                      <  1:729515/251‑2 (MQ=255)   aaaTGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATgcat                                                                                                                                                                                                                                     <  1:636045/251‑3 (MQ=255)           aagaaaaagaaaGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAAcc                                                                                                                                                                                                                             <  1:743555/251‑2 (MQ=255)                       gCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGtgtg                                                                                                                                                                                                                 >  1:490513/1‑248 (MQ=255)                             aCATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGtgtgtttgta                                                                                                                                                                                                           <  1:21011/251‑10 (MQ=255)                              cATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTa                                                                                                                                                                                                                                          >  1:811094/1‑219 (MQ=255)                              cATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGtgtgtttgtat                                                                                                                                                                                                          >  1:19963/1‑241 (MQ=255)                                   gagGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGtgtgtttgtatta                                                                                                                                                                                                        >  1:305531/1‑236 (MQ=255)                                    agGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGtgtgtttgtattattt                                                                                                                                                                                                     <  1:500681/250‑16 (MQ=255)                                                   cTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCATCATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGtgtgtttgtatt                                                                                                                                                                                                         <  1:897039/231‑12 (MQ=255)                                                   cTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTgg                                                                                                                                                                                                                     >  1:557822/1‑219 (MQ=255)                                                       aatcgTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGaag                                                                                                                                                                                 <  1:785683/247‑2 (MQ=255)                                                             agcatgtTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAAtt                                                                                                                                                                           >  1:465374/5‑251 (MQ=255)                                                                               tttctttttcttTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCagcataggaaagaaa                                                                                                                                                          <  1:273/250‑15 (MQ=255)                                                                                       tctttATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATgc                                                                                                                                                                                                                                       >  1:247894/1‑165 (MQ=255)                                                                                         tttATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCagcataggaaagaaa                                                                                                                                                          >  1:693126/1‑226 (MQ=255)                                                                                                  tttGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGAc                                                                                                                                        <  1:279608/249‑1 (MQ=255)                                                                                                   ttGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTa                                                                                                                                     >  1:746596/1‑251 (MQ=255)                                                                                                                  aCTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTgg                                                                                                                                                                                                                     >  1:138904/1‑156 (MQ=255)                                                                                                                  aCTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTAc                                                                                                                                                   <  1:719046/222‑1 (MQ=255)                                                                                                                              taattaatGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCaaagtaatgatgaaatagcca                                                                                                          <  1:194400/251‑20 (MQ=255)                                                                                                                                 ttaatGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCagcataggaaagaaattatt                                                                                                                                                     <  1:496282/205‑20 (MQ=255)                                                                                                                                        cccTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGtgtgtttgtatt                                                                                                                                                                                                         <  1:668872/146‑12 (MQ=255)                                                                                                                                        cccTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCagcataggaa                                                                                                                                                               <  1:852618/188‑10 (MQ=255)                                                                                                                                            aCCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAACTTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCaaagt                                                                                                                          >  1:439354/1‑218 (MQ=255)                                                                                                                                                gTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTAc                                                                                                                                                   <  1:163611/192‑1 (MQ=255)                                                                                                                                                       cGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCagcat                                                                                                                                                                    <  1:606635/168‑5 (MQ=255)                                                                                                                                                        gTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATgcattatta                                                                                                                                                                                                                                <  1:713004/107‑8 (MQ=255)                                                                                                                                                         tGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAAttgaac                                                                               <  1:460298/251‑1 (MQ=255)                                                                                                                                                               attatGTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATgcattatta                                                                                                                                                                                                                                >  1:894091/1‑93 (MQ=255)                                                                                                                                                                    gTTTTTGGTAGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCaaagtaatga                                                                                                                     >  1:791350/1‑194 (MQ=255)                                                                                                                                                                         tggtaGTGAGCTTTTCGGTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACtgat                                                               <  1:343804/247‑4 (MQ=255)                                                                                                                                                                                          gTATGGGAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTAc                                                                   <  1:877369/230‑1 (MQ=255)                                                                                                                                                                                              gggAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAACTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATcccgttttaaa                                          >  1:889899/1‑242 (MQ=255)                                                                                                                                                                                                gAATTATTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCaaagtaatgatgaaatagcc                                                                                                           >  1:172888/1‑166 (MQ=255)                                                                                                                                                                                                     aTTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGgag                                                                                                                                              >  1:637747/1‑144 (MQ=255)                                                                                                                                                                                                     aTTTGGTGGTTGATGAGGAAGTCAACATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATtaacaat                                                        >  1:258979/1‑230 (MQ=255)                                                                                                                                                                                                                             aCATGATAATTAAGCTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATcc                                                   >  1:318174/1‑211 (MQ=255)                                                                                                                                                                                                                                         agcTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATTAACAATAATCCCGTTTTAAAAATGCTTGGAATTATTGATAAAAATATAAAAGTAAAAtta  >  1:170225/3‑247 (MQ=255)                                                                                                                                                                                                                                          gcTATTACTGTCTTATGCATTATTAACCATTTTTGGTGTGTTTGTATTATTTTTGGCACTTTCAGCTAGAAGAAAATTCCAGCATAGGAAAGAAATTATTACTGGAGAAAGACCTAAGGATCAAAGTAATGATGAAATAGCCAAAGGTAAAAGCAAGCAGCAAATTGAACTTGAGCTGTTACTGATtaacaat                                                        >  1:177660/2‑193 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                TAAAATGGAGAAGAAAAAGAAAGCAATGACATCAGAGGCTAGAATGTCAGCTATAATTGTAGCGTGTTTTCCTGTCGGTTTCTTTTTCTTTATGCAATTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGCAGTGAACTTTTCGGTATGGGAATCATTTGGTGGTTGATGAGGAAGTCAACATGATGATTAAACTATTATTATCTTATGCGCTATTAACGATTTTTGGTATTATTGTATTGTTTGCCGCACTTTCAGCCAGAAAAAAATTCCAATATAGAAAGGAAGTTATTACTGGAGAAAGACCTAAGGATCAAGATAGTGACGAAGTAGCTAAAAATAAGAGCAAACAGCAAATTGAACTTGAACTATTACTAATTAATAATAATCCTATCTTAAAAGTACTCGGAATTATTGATAAAAATATAAAAGTAAAATTG  >  1/1484994‑1485473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑