Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,484,940	C→T	G203G (GGC→GGT)	unknown →	Flp pilus assembly protein TadB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,484,940	0	C	T	100.0%	144.1 / NA	53	G203G (GGC→GGT)	unknown	Flp pilus assembly protein TadB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/22);  total (31/22)

CCGGAGGTAATTCAGATTCTCAATGCAGCGGCAACTTCAGGTGCTGGCTTATTACAAGCATTAGAACGCTGCGGTAAGGATCTTACCGGTCAATTAGGTGAGGAGTTTAAGAGTATCCATAAGCGTATTGCAATAGGCGAGGATCCTGTTTCAGTTTTTGAAGACAGCTATGATCGTTACCCATATAAGGAATTCTATTTCTTCATAACTATTGTACGCACCAACTTAAGTAAAGGCGGACAGATACGCGAGGTTATTTCCCGTTTAGGTCGTGTAATTGCCGATAGCAATAAAATGGAGAAGAAAAAGAAAGCAATGACATCAGAGGCTAGAATGTCAGCTATAATTGTAGCGTGTTTTCCTGTCGGTTTCTTTTTCTTTATGCAATTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGCAGTGAACTTTTCGGTATGGGA  >  1/1484704‑1485186                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       ccagAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATAcggga                                                                                                                                                                                                                                          <  1:393986/248‑4 (MQ=255)      ggTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTa                                                                                                                                                                                                                                     >  1:842261/1‑251 (MQ=255)                                              ggCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGATTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAAt                                                                                                                                                                                             >  1:573281/1‑251 (MQ=255)                                              ggCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAAt                                                                                                                                                                                             >  1:213886/1‑251 (MQ=255)                                                                              ggATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAgg                                                                                                                                                             >  1:38079/1‑251 (MQ=255)                                                                                                                                 ctctataggggaggaccccgctacAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGtttcttttt                                                                                                            >  1:758549/24‑249 (MQ=255)                                                                                                                                   ctataggggaggaccccgctacAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTttctttttcttt                                                                                                        >  1:12717/22‑251 (MQ=255)                                                                                                                                   ctataggggaggaccccgctacAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTttctttttcttt                                                                                                        >  1:89796/22‑251 (MQ=255)                                                                                                                                   ctataggggaggaccccgctacAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTttctttttcttt                                                                                                        >  1:262085/22‑251 (MQ=255)                                                                                                                                                 cccgctacAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTAt                                                                                          <  1:419918/244‑1 (MQ=255)                                                                                                                                                   cgctacAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGc                                                                                        <  1:319564/246‑1 (MQ=255)                                                                                                                                                           tttttGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGaaa                                                                                  <  1:800048/249‑1 (MQ=255)                                                                                                                                                                     taGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGGCAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTAt                                                                                          <  1:666186/230‑1 (MQ=255)                                                                                                                                                                      aGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATaa                                                                                                                                                                                                <  1:844472/128‑1 (MQ=255)                                                                                                                                                                      aGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCtt                                                                     >  1:122466/1‑251 (MQ=255)                                                                                                                                                                       gCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCtta                                                                    >  1:894751/1‑251 (MQ=255)                                                                                                                                                                        cTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCttaa                                                                   >  1:252842/1‑251 (MQ=255)                                                                                                                                                                          aTGATCGTTACCCTTATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCttaatt                                                                 >  1:159390/1‑251 (MQ=255)                                                                                                                                                                             aTCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTtaattaat                                                              >  1:638598/1‑251 (MQ=255)                                                                                                                                                                                     ccATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGtt                                                                                                                               <  1:881776/178‑1 (MQ=255)                                                                                                                                                                                      cATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAgg                                                                                                                                                             >  1:267094/1‑147 (MQ=255)                                                                                                                                                                                      cATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAgg                                                                                                                                                             <  1:413460/147‑1 (MQ=255)                                                                                                                                                                                      cATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGcatg                                                                                                                                 <  1:831937/175‑4 (MQ=255)                                                                                                                                                                                       atatAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGca                                                                                                                                   <  1:810491/172‑2 (MQ=255)                                                                                                                                                                                       atatAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGAc                                                           >  1:344959/1‑244 (MQ=255)                                                                                                                                                                                       atatAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCg                                                    <  1:753931/251‑1 (MQ=255)                                                                                                                                                                                        tataAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCatgt                                                                                                                                <  1:483872/174‑1 (MQ=255)                                                                                                                                                                                              gagttttactttttcATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGa                                               <  1:476528/237‑1 (MQ=255)                                                                                                                                                                                                 ttttactttttcATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGTGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACg                                           <  1:427062/241‑1 (MQ=255)                                                                                                                                                                                                            cATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATg                               <  1:414653/251‑1 (MQ=255)                                                                                                                                                                                                              tAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGc                                        <  1:829197/240‑1 (MQ=255)                                                                                                                                                                                                               aaCTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGGCCCTACCGGTAGATACGTGCTTTATTATGttt                            <  1:416822/251‑1 (MQ=255)                                                                                                                                                                                                                 cTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGAttt                                                                        <  1:880998/205‑1 (MQ=255)                                                                                                                                                                                                                 cTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGttttt                          >  1:428846/1‑251 (MQ=255)                                                                                                                                                                                                                 cTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGttttt                          >  1:858964/1‑251 (MQ=255)                                                                                                                                                                                                                 cTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGttttt                          >  1:493698/1‑251 (MQ=255)                                                                                                                                                                                                                 cTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGttttt                          >  1:455380/1‑251 (MQ=255)                                                                                                                                                                                                                 cTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGttttt                          >  1:434424/1‑251 (MQ=255)                                                                                                                                                                                                                   aTTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATg                               <  1:198605/244‑1 (MQ=255)                                                                                                                                                                                                                    ttGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGtttctttt                                                                                                             >  1:818790/1‑165 (MQ=255)                                                                                                                                                                                                                    ttGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTggt                       <  1:48413/251‑2 (MQ=255)                                                                                                                                                                                                                    ttGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTggt                       <  1:30189/251‑2 (MQ=255)                                                                                                                                                                                                                           cactaatttaaacaaaGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGagc                <  1:23726/238‑3 (MQ=255)                                                                                                                                                                                                                           cactaatttaaacaaaGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGag                 >  1:165723/14‑249 (MQ=255)                                                                                                                                                                                                                               aatttaaacaaaGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAgg                                                                                                                                                             >  1:842338/10‑106 (MQ=255)                                                                                                                                                                                                                               aatttaaacaaaGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAAt                                                                                                                                                      >  1:75285/10‑113 (MQ=255)                                                                                                                                                                                                                               aatttaaacaaaGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGt                              >  1:3581/10‑233 (MQ=255)                                                                                                                                                                                                                               aatttaaacaaaGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTggt                       >  1:394928/10‑239 (MQ=255)                                                                                                                                                                                                                               aatttaaacaaaGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCtttt            >  1:406210/10‑251 (MQ=255)                                                                                                                                                                                                                               aatttaaacaaaGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCtttt            >  1:869277/10‑251 (MQ=255)                                                                                                                                                                                                                               aatttaaacaaaGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCtttt            >  1:379835/10‑251 (MQ=255)                                                                                                                                                                                                                                       caaaGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATgg    >  1:264799/2‑251 (MQ=255)                                                                                                                                                                                                                                         aaGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATGATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGTAGTGAGCTTTTCGGTATGGGa  >  1:568092/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       CCGGAGGTAATTCAGATTCTCAATGCAGCGGCAACTTCAGGTGCTGGCTTATTACAAGCATTAGAACGCTGCGGTAAGGATCTTACCGGTCAATTAGGTGAGGAGTTTAAGAGTATCCATAAGCGTATTGCAATAGGCGAGGATCCTGTTTCAGTTTTTGAAGACAGCTATGATCGTTACCCATATAAGGAATTCTATTTCTTCATAACTATTGTACGCACCAACTTAAGTAAAGGCGGACAGATACGCGAGGTTATTTCCCGTTTAGGTCGTGTAATTGCCGATAGCAATAAAATGGAGAAGAAAAAGAAAGCAATGACATCAGAGGCTAGAATGTCAGCTATAATTGTAGCGTGTTTTCCTGTCGGTTTCTTTTTCTTTATGCAATTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCGGTAGATACGTGCTTTATTATGTTTTTGGCAGTGAACTTTTCGGTATGGGA  >  1/1484704‑1485186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑