Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,484,901	T→C	Y190Y (TAT→TAC)	unknown →	Flp pilus assembly protein TadB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,484,901	0	T	C	100.0%	97.4 / NA	36	Y190Y (TAT→TAC)	unknown	Flp pilus assembly protein TadB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/16);  total (20/16)

GGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCGGAGGTAATTCAGATTCTCAATGCAGCGGCAACTTCAGGTGCTGGCTTATTACAAGCATTAGAACGCTGCGGTAAGGATCTTACCGGTCAATTAGGTGAGGAGTTTAAGAGTATCCATAAGCGTATTGCAATAGGCGAGGATCCTGTTTCAGTTTTTGAAGACAGCTATGATCGTTACCCATATAAGGAATTCTATTTCTTCATAACTATTGTACGCACCAACTTAAGTAAAGGCGGACAGATACGCGAGGTTATTTCCCGTTTAGGTCGTGTAATTGCCGATAGCAATAAAATGGAGAAGAAAAAGAAAGCAATGACATCAGAGGCTAGAATGTCAGCTATAATTGTAGCGTGTTTTCCTGTCGGTTTCTTTTTCTTTATGCAATTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCG  >  1/1484665‑1485136                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            ggTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGATGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTAt                                                                                                                                                                                                                               >  1:238451/1‑251 (MQ=255)      gTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTa                                                                                                                                                                                                                           >  1:579626/1‑250 (MQ=255)      gTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTa                                                                                                                                                                                                                           >  1:290066/1‑250 (MQ=255)       tACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTAc                                                                                                                                                                                                                          <  1:271444/250‑1 (MQ=255)              ataaAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAAc                                                                                                                                                                                                                                  >  1:549062/1‑235 (MQ=255)                aaaaaaaCATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCActaattt                                                                                                                                                                                                                <  1:678777/251‑7 (MQ=255)                    aaaCATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTaaac                                                                                                                                                                                                            <  1:351924/251‑3 (MQ=255)                                       tccagAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGc                                                                                                                                                                                                                        <  1:408478/216‑1 (MQ=255)                                        ccagAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATAcggga                                                                                                                                                                                        <  1:393986/248‑4 (MQ=255)                                             ggTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTa                                                                                                                                                                                   >  1:842261/1‑251 (MQ=255)                                                                                     ggCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGATTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAAt                                                                                                                                           >  1:573281/1‑251 (MQ=255)                                                                                     ggCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAAt                                                                                                                                           >  1:213886/1‑251 (MQ=255)                                                                                                                     ggATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAgg                                                                                                           >  1:38079/1‑251 (MQ=255)                                                                                                                                                                        ctctataggggaggaccccgctacAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGtttcttttt                                                          >  1:758549/24‑249 (MQ=255)                                                                                                                                                                          ctataggggaggaccccgctacAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTttctttttcttt                                                      >  1:12717/22‑251 (MQ=255)                                                                                                                                                                          ctataggggaggaccccgctacAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTttctttttcttt                                                      >  1:89796/22‑251 (MQ=255)                                                                                                                                                                          ctataggggaggaccccgctacAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTttctttttcttt                                                      >  1:262085/22‑251 (MQ=255)                                                                                                                                                                                        cccgctacAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTAt                                        <  1:419918/244‑1 (MQ=255)                                                                                                                                                                                          cgctacAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGc                                      <  1:319564/246‑1 (MQ=255)                                                                                                                                                                                                  tttttGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGaaa                                <  1:800048/249‑1 (MQ=255)                                                                                                                                                                                                            taGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGGCAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTAt                                        <  1:666186/230‑1 (MQ=255)                                                                                                                                                                                                             aGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTaaac                                                                                                                                                                                                            >  1:328792/1‑64 (MQ=255)                                                                                                                                                                                                             aGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATaa                                                                                                                                              <  1:844472/128‑1 (MQ=255)                                                                                                                                                                                                             aGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCtt                   >  1:122466/1‑251 (MQ=255)                                                                                                                                                                                                              gCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCtta                  >  1:894751/1‑251 (MQ=255)                                                                                                                                                                                                               cTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCttaa                 >  1:252842/1‑251 (MQ=255)                                                                                                                                                                                                                 aTGATCGTTACCCTTATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCttaatt               >  1:159390/1‑251 (MQ=255)                                                                                                                                                                                                                    aTCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTtaattaat            >  1:638598/1‑251 (MQ=255)                                                                                                                                                                                                                            ccATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGtt                                                                             <  1:881776/178‑1 (MQ=255)                                                                                                                                                                                                                             cATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAgg                                                                                                           <  1:413460/147‑1 (MQ=255)                                                                                                                                                                                                                             cATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAgg                                                                                                           >  1:267094/1‑147 (MQ=255)                                                                                                                                                                                                                             cATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGcatg                                                                               <  1:831937/175‑4 (MQ=255)                                                                                                                                                                                                                              atatAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGca                                                                                 <  1:810491/172‑2 (MQ=255)                                                                                                                                                                                                                              atatAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGAc         >  1:344959/1‑244 (MQ=255)                                                                                                                                                                                                                              atatAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCg  <  1:753931/251‑1 (MQ=255)                                                                                                                                                                                                                               tataAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCatgt                                                                              <  1:483872/174‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            GGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCGGAGGTAATTCAGATTCTCAATGCAGCGGCAACTTCAGGTGCTGGCTTATTACAAGCATTAGAACGCTGCGGTAAGGATCTTACCGGTCAATTAGGTGAGGAGTTTAAGAGTATCCATAAGCGTATTGCAATAGGCGAGGATCCTGTTTCAGTTTTTGAAGACAGCTATGATCGTTACCCATATAAGGAATTCTATTTCTTCATAACTATTGTACGCACCAACTTAAGTAAAGGCGGACAGATACGCGAGGTTATTTCCCGTTTAGGTCGTGTAATTGCCGATAGCAATAAAATGGAGAAGAAAAAGAAAGCAATGACATCAGAGGCTAGAATGTCAGCTATAATTGTAGCGTGTTTTCCTGTCGGTTTCTTTTTCTTTATGCAATTTGCTATGCCGGAAAACTTTGATTTCTTAATTAATGACCCTACCG  >  1/1484665‑1485136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑