Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,484,865	A→C	E178D (GAA→GAC)	unknown →	Flp pilus assembly protein TadB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,484,865	0	A	C	100.0%	65.5 / NA	25	E178D (GAA→GAC)	unknown	Flp pilus assembly protein TadB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (15/10);  total (15/10)

GTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCGGAGGTAATTCAGATTCTCAATGCAGCGGCAACTTCAGGTGCTGGCTTATTACAAGCATTAGAACGCTGCGGTAAGGATCTTACCGGTCAATTAGGTGAGGAGTTTAAGAGTATCCATAAGCGTATTGCAATAGGCGAGGATCCTGTTTCAGTTTTTGAAGACAGCTATGATCGTTACCCATATAAGGAATTCTATTTCTTCATAACTATTGTACGCACCAACTTAAGTAAAGGCGGACAGATACGCGAGGTTATTTCCCGTTTAGGTCGTGTAATTGCCGATAGCAATAAAATGGAGAAGAAAAAGAAAGCAATGACATCAGAGGCTAGAATGTCAGCTATAATTGTAGCGTGTTTTCCTGTCGGTTTCTTTTTCTTTATGCAATTTGCTATGCCGGAAA  >  1/1484644‑1485106                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  gttttgtttATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGa                                                                                                                                                                                                                      >  1:816286/1‑251 (MQ=255)                      ggTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAgg                                                                                                                                                                                                                       <  1:482687/229‑1 (MQ=255)                      ggTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGATGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTAt                                                                                                                                                                                                 >  1:238451/1‑251 (MQ=255)                           gTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAgg                                                                                                                                                                                                                       >  1:771087/1‑224 (MQ=255)                           gTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAgg                                                                                                                                                                                                                       <  1:513144/224‑1 (MQ=255)                           gTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGag                                                                                                                                                                                                                     >  1:617992/1‑225 (MQ=255)                           gTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTa                                                                                                                                                                                             >  1:579626/1‑250 (MQ=255)                           gTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTa                                                                                                                                                                                             >  1:290066/1‑250 (MQ=255)                            tACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTAc                                                                                                                                                                                            <  1:271444/250‑1 (MQ=255)                                   ataaAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAAc                                                                                                                                                                                                    >  1:549062/1‑235 (MQ=255)                                     aaaaaaaCATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCActaattt                                                                                                                                                                                  <  1:678777/251‑7 (MQ=255)                                         aaaCATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTaaac                                                                                                                                                                              <  1:351924/251‑3 (MQ=255)                                                            tccagAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGc                                                                                                                                                                                          <  1:408478/216‑1 (MQ=255)                                                             ccagAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATAcggga                                                                                                                                                          <  1:393986/248‑4 (MQ=255)                                                                  ggTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTa                                                                                                                                                     >  1:842261/1‑251 (MQ=255)                                                                                                          ggCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGATTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAAt                                                                                                             >  1:573281/1‑251 (MQ=255)                                                                                                          ggCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAAt                                                                                                             >  1:213886/1‑251 (MQ=255)                                                                                                                                          ggATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAgg                                                                             >  1:38079/1‑251 (MQ=255)                                                                                                                                                                                             ctctataggggaggaccccgctacAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGtttcttttt                            >  1:758549/24‑249 (MQ=255)                                                                                                                                                                                               ctataggggaggaccccgctacAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTttctttttcttt                        >  1:262085/22‑251 (MQ=255)                                                                                                                                                                                               ctataggggaggaccccgctacAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTttctttttcttt                        >  1:12717/22‑251 (MQ=255)                                                                                                                                                                                               ctataggggaggaccccgctacAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTttctttttcttt                        >  1:89796/22‑251 (MQ=255)                                                                                                                                                                                                             cccgctacAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTAt          <  1:419918/244‑1 (MQ=255)                                                                                                                                                                                                               cgctacAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGc        <  1:319564/246‑1 (MQ=255)                                                                                                                                                                                                                       tttttGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTAAACAAAGGTGGACAGATACGGGAGGTTATTTCTCGTTTAGGTCGCGTAATTGCGGATAGCAATAAAATGGAAAAGAAAAAGAAAGCAATGACATCAGAGGCCAGAATGTCAGCTATAATCGTAGCATGTTTTCCGGTCGGTTTCTTTTTCTTTATGCAGTTTGCTATGCCGGaaa  <  1:800048/249‑1 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  GTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCGGAGGTAATTCAGATTCTCAATGCAGCGGCAACTTCAGGTGCTGGCTTATTACAAGCATTAGAACGCTGCGGTAAGGATCTTACCGGTCAATTAGGTGAGGAGTTTAAGAGTATCCATAAGCGTATTGCAATAGGCGAGGATCCTGTTTCAGTTTTTGAAGACAGCTATGATCGTTACCCATATAAGGAATTCTATTTCTTCATAACTATTGTACGCACCAACTTAAGTAAAGGCGGACAGATACGCGAGGTTATTTCCCGTTTAGGTCGTGTAATTGCCGATAGCAATAAAATGGAGAAGAAAAAGAAAGCAATGACATCAGAGGCTAGAATGTCAGCTATAATTGTAGCGTGTTTTCCTGTCGGTTTCTTTTTCTTTATGCAATTTGCTATGCCGGAAA  >  1/1484644‑1485106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑