Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	262,131	A→G	N101D (AAC→GAC)	unknown →	Cell envelope opacity‑associated protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	262,131	0	A	G	100.0%	197.0 / NA	71	N101D (AAC→GAC)	unknown	Cell envelope opacity‑associated protein A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/31);  total (40/31)

ATTGGATTTGGGCTTTAGTCAAATGGAACCGATCACCCCGAAAAAAGTGATTAAACCGGAACCGTCTTTGTTTGAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCAACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTGGGCGATTTTAAGTATTCTCCCGCAAAAACATCGTCGCATTTTCGTCGCGCTATTCGGCGTGGTGTTGGTGCTGATTTTTATCT  >  1/261884‑262374                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    aTTGGATTTGGGCTTTAGTCAAATGGAACCGATCACCCCGAAAAAAGTGATTAAACCGGAACCGTCTTTGTTTGAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCG‑AAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGcgacga                                                                                                                                                                                                                                                 >  1:588547/1‑251 (MQ=255)     gATTTGGGCTTTAGTCAAATGGAACCGATCACCCCGAAAAAAGTGATTAAACCGGAACCGTCTTTGTTTGAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAAc                                                                                                                                                                                                                                               >  1:409902/1‑250 (MQ=255)     gATTTGGGCTTTAGTCAAATGGAACCGATCACCCCGAAAAAAGTGATTAAACCGGAACCGTCTTTGTTTGAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAAc                                                                                                                                                                                                                                               <  1:585680/250‑1 (MQ=255)           ggCTTTAGTCAAATGGAACCGATCACCCCGAAAAAAGTGATTAAACCGGAACCGTCTTTGTTTGAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGAt                                                                                                                                                                                                                                        >  1:389135/1‑251 (MQ=255)           ggCTTTAGTCAAATGGAACCGATCACCCCGAAAAAAGTGATTAAACCGGAACCGTCTTTGTTTGAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGAt                                                                                                                                                                                                                                        >  1:670221/1‑251 (MQ=255)            gCTTTAGTCAAATGGAACCGATCACCCCGAAAAAAGTGATTAAACCGGAACCGTCTTTGTTTGAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGAtt                                                                                                                                                                                                                                       >  1:96871/1‑251 (MQ=255)                 aGTCAAATGGAACCGATCACCCCGAAAAAAGTGATTAAACCGGAACCGTCTTTGTTTGAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGAt                                                                                                                                                                                                                                        <  1:748174/245‑1 (MQ=255)                          gAACCGATCACCCCGAAAAAAGTGATTAAACCGGAACCGTCTTTGTTTGAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGcgacga                                                                                                                                                                                                                                                 >  1:70430/1‑227 (MQ=255)                            aCCGATCACCCCGAAAAAAGTGATTAAACCGGAACCGTCTTTGTTTGAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGa                                                                                                                                                                                                                       >  1:187110/1‑251 (MQ=255)                                   aCCCCGAAAAAAGTGATTAAACCGGAACCGTCTTTGTTTGAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGaaa                                                                                                                                                                                                                 >  1:502010/1‑250 (MQ=255)                                     cccGAAAAAAGTGATTAAACCGGAACCGTCTTTGTTTGAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAAccc                                                                                                                                                                                                              <  1:23999/251‑1 (MQ=255)                                        gAAAAAAGTGATTAAACCGGAACCGTCTTTGTTTGAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGt                                                                                                                                                                                                           >  1:614222/1‑251 (MQ=255)                                           aaaaGTGATTAAACCGGAACCGTCTTTGTTTGAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAg                                                                                                                                                                                                        <  1:104550/251‑1 (MQ=255)                                            aaaGTGATTAAACCGGAACCGTCTTTGTTTGAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGa                                                                                                                                                                                                       >  1:281891/1‑251 (MQ=255)                                                tGATTAAACCGGAACCGTCTTTGTTTGAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGc                                                                                                                                                                                                    <  1:295857/250‑1 (MQ=255)                                                      aaCCGGAACCGTCTTTGTTTGAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGa                                                                                                                                                                                              >  1:824095/1‑250 (MQ=255)                                                      aaCCGGAACCGTCTTTGTTTGAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGa                                                                                                                                                                                              >  1:573168/1‑250 (MQ=255)                                                      aaCCGGAACCGTCTTTGTTTGAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGAc                                                                                                                                                                                             <  1:27263/251‑1 (MQ=255)                                                                gTCTTTGTTTGAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCg                                                                                                                                                                                   >  1:157338/1‑251 (MQ=255)                                                                    ttgtttgAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAGTATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTAc                                                                                                                                                                               <  1:855901/251‑1 (MQ=255)                                                                    ttgtttgAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTAc                                                                                                                                                                               <  1:23828/251‑1 (MQ=255)                                                                      gtttgAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCg                                                                                                                                                                             >  1:358942/1‑251 (MQ=255)                                                                         tgAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAg                                                                                                                                                                          <  1:842901/251‑1 (MQ=255)                                                                               gTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGgaag                                                                                                                                                                    >  1:499010/1‑251 (MQ=255)                                                                                           aGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCg                                                                                                                                                                                   >  1:77163/1‑224 (MQ=255)                                                                                            ggCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTAc                                                                                                                                                                               <  1:730211/227‑1 (MQ=255)                                                                                               aaaGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCg                                                                                                                                                                                                                        >  1:782712/1‑183 (MQ=255)                                                                                                   aTCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAacac                                                                                                                                                  <  1:863795/249‑1 (MQ=255)                                                                                                    tctcCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAAc                                                                                                                                               <  1:593731/251‑1 (MQ=255)                                                                                                     ctcCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAAcc                                                                                                                                              <  1:893246/251‑1 (MQ=255)                                                                                                                gaaagaaCAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGCCCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGt                                                                                                                                                                            <  1:769654/210‑1 (MQ=255)                                                                                                                    gaaCAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGtt                                                                                                                                                                                 <  1:824310/201‑1 (MQ=255)                                                                                                                    gaaCAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAAtt                                                                                                                               >  1:160466/1‑251 (MQ=255)                                                                                                                     aaCAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAAt                                                                                                                                <  1:718408/249‑1 (MQ=255)                                                                                                                     aaCAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAAt                                                                                                                                <  1:120399/249‑1 (MQ=255)                                                                                                                         aaaGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTgaaag                                                                                                                          >  1:725313/1‑251 (MQ=255)                                                                                                                            gAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTgaaagaaa                                                                                                                       >  1:658790/1‑251 (MQ=255)                                                                                                                            gAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTg                                                                                                                              >  1:658921/1‑244 (MQ=255)                                                                                                                                        tCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGgaagaa                                                                                                                                                                  >  1:18511/1‑196 (MQ=255)                                                                                                                                        tCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCg                                                                                                           >  1:283855/1‑251 (MQ=255)                                                                                                                                                  gTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAAcc                                                                                                                                              >  1:808122/1‑206 (MQ=255)                                                                                                                                                       cAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATc                                                                                               >  1:164494/1‑248 (MQ=255)                                                                                                                                                           gaACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGcc                                                                                                                                                                                                   <  1:240697/144‑1 (MQ=255)                                                                                                                                                           gaACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGaa                                                                                                                                          >  1:203853/1‑201 (MQ=255)                                                                                                                                                           gaACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGcc                                                                                                            >  1:329124/1‑231 (MQ=255)                                                                                                                                                           gaACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGaaaa                                                                                        >  1:680863/1‑251 (MQ=255)                                                                                                                                                            aaCCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAAt                                                                                       <  1:626220/251‑1 (MQ=255)                                                                                                                                                              cccGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTg                                                                                     >  1:78446/1‑251 (MQ=255)                                                                                                                                                                 gTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAAccc                                                                                                                                                                                                              >  1:271091/1‑127 (MQ=255)                                                                                                                                                                 gTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAAcc                                                                                                                                        <  1:599611/197‑1 (MQ=255)                                                                                                                                                                 gTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTggg                                                                                   >  1:642568/1‑250 (MQ=255)                                                                                                                                                                  tATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTGGGc                                                                                  <  1:116654/250‑1 (MQ=255)                                                                                                                                                                       ggtggtGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGCAAGCCGGTTTTAAAAATCCTGAAAATTGGGCGAtttt                                                                            >  1:828081/1‑251 (MQ=255)                                                                                                                                                                        gtggtgCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTGGGCGATTTTa                                                                           >  1:254695/1‑251 (MQ=255)                                                                                                                                                                        gtggtgCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGG‑TTTAAAAATCCTGAAAAt                                                                                       >  1:754955/1‑238 (MQ=255)                                                                                                                                                                           gtgCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAAcc                                                                                                                                                                                                               >  1:880892/1‑116 (MQ=255)                                                                                                                                                                           gtgCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTGGGCGATTTTAAGt                                                                        >  1:272470/1‑251 (MQ=255)                                                                                                                                                                           gtgCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTGGGCGATTTTAAGt                                                                        >  1:291919/1‑251 (MQ=255)                                                                                                                                                                            tgCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTGGGCGATTTTAAGTa                                                                       <  1:114677/251‑1 (MQ=255)                                                                                                                                                                             gCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTGGGCGATTTTAAGTAt                                                                      >  1:476525/1‑251 (MQ=255)                                                                                                                                                                              ccGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTGGGCGATTTTAAGTAt                                                                      <  1:201023/250‑1 (MQ=255)                                                                                                                                                                              ccGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTGGGCGATTTTAAGTAt                                                                      <  1:402026/250‑1 (MQ=255)                                                                                                                                                                                       cTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTGGGCGATTTTAAGTATTCTCCCGCaa                                                            >  1:818840/1‑251 (MQ=255)                                                                                                                                                                                        tGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTGGGCGATTTTAAGTATTCTCCCGCaaa                                                           <  1:710284/251‑1 (MQ=255)                                                                                                                                                                                                  aGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAAt                                                                                       <  1:826761/213‑1 (MQ=255)                                                                                                                                                                                                            gCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTGGGCGATTTTAAGTATTCTCCCGCAAAAACATCGTCGCATTTtcgt                                        >  1:516561/1‑250 (MQ=255)                                                                                                                                                                                                                     cgaccgaGAGATTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTGGGCGATTTTAAGTATTCTCCCGCAAAAACATCGTCGCATTTTCGTCGCGCTATTc                              <  1:364478/251‑1 (MQ=255)                                                                                                                                                                                                                           agagaTTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTGGGCGATTTTAAGTATTCTCCCGCAAAAACATCGTCGCATTTTCGTCGCGCTATTCGGCgt                         <  1:847460/250‑1 (MQ=255)                                                                                                                                                                                                                               aTTTTCAATAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTGGGCGATTTTAAGTATTCTCCCGCAAAAACATCGTCGCATTTTCGTCGCGCTATTCGGCGTGgtgt                    >  1:522525/1‑251 (MQ=255)                                                                                                                                                                                                                                     aaTAGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTGGGCGATTTTAAGTATTCTCCCGCAAAAACATCGTCGCATTTTCGTCGCGCTATTCGGCGTGGTGTTGGTGc              <  1:142922/251‑1 (MQ=255)                                                                                                                                                                                                                                        aGAAAACACAGAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTGGGCGATTTTAAGTATTCTCCCGCAAAAACATCGTCGCATTTTCGTCGCGCTATTCGGCGTGGTGTTGGTGc              >  1:189312/1‑248 (MQ=255)                                                                                                                                                                                                                                                  gAAAGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTGGGCGATTTTAAGTATTCTCCCGCAAAAACATCGTCGCATTTTCGTCGCGCTATTCGGCGTGGTGTTGGTGCTGATTTTTATCt  <  1:543806/250‑1 (MQ=255)                                                                                                                                                                                                                                                   aaaGCGACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTGGGCGATTTTAAGTATTCTCCCGCAAAAACATCGTCGCATTTTCGTCGCGCTATTCGGCGTGGTGTTGGTGCTGATTTTTATc   <  1:661769/248‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    ATTGGATTTGGGCTTTAGTCAAATGGAACCGATCACCCCGAAAAAAGTGATTAAACCGGAACCGTCTTTGTTTGAGAAGTTGCTTGGAAAAGGCAAAGATCTCCTTGCGAAGAAAGAACAAAAGAATATGGAAAATCAATTTGCCGTTCGCAGAGAACCCGTATTCGGTGGTGCCGGTGCGACTGGAGAGAAAAGCATGACAAGCGAAAACCCGACCGAGAGATTTTCAATAGAAAACACAGAAAGCAACGAACCGCAGATTGCCGCCGACACGGCGACGGAAACCCCGTCAGAAGCCCCGGACGAACCTCTCGTTACCGTAGCGGAAGAAGCCGTTGAAGCAACACAACCGGAACCTGCCGAATTGAAAGAAAAACCGAAAGCCGGTTTTAAAAATCCTGAAAATTGGGCGATTTTAAGTATTCTCCCGCAAAAACATCGTCGCATTTTCGTCGCGCTATTCGGCGTGGTGTTGGTGCTGATTTTTATCT  >  1/261884‑262374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑