Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,484,706	G→A	P125P (CCG→CCA)	unknown →	Flp pilus assembly protein TadB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,484,706	0	G	A	100.0%	139.2 / NA	53	P125P (CCG→CCA)	unknown	Flp pilus assembly protein TadB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/27);  total (26/27)

TTTTTTTAAGAGCCTCAAATCAAAAATTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAGAAACTTGATTCGCAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGATCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCGGAGGTAATTCAGATTCTCAATGCAGCGGCAACTTCAGGTGCTGGCTTATTACAAGCATTAGAACGCTGCGGTAAGGATCTTACCGGTCAATTAGGTGAGGAGTTTAAGAGTATCCATAAGCGTATTGCAATAGGCGAGGATCCTGTTTCAGTTTTTGAAGACAGCTATGATCGTTACCCATATAAGGAATTCTATTTCTTCATAACTATTGTACGCACCAACTTAAGT  >  1/1484460‑1484934                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                     tttttttAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAgg                                                                                                                                                                                                                                  <  1:225040/251‑1 (MQ=255)                                             aCTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTctagcg                                                                                                                                                                                              <  1:484854/243‑6 (MQ=255)                                              cTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAgg                                                                                                                                                                                                                                  >  1:255825/1‑206 (MQ=255)                                                                          gttCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTctagcgtaggcttattac                                                                                                                                                                                  >  1:160979/2‑209 (MQ=255)                                                                              gtaaTATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTctagcgtaggcttattac                                                                                                                                                                                  >  1:125682/3‑205 (MQ=255)                                                                                aaTATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCtaac                                                                                                                                                   <  1:742711/251‑4 (MQ=255)                                                                                aaTATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCtaac                                                                                                                                                   <  1:227566/251‑4 (MQ=255)                                                                                 atatTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTctagcgtaggcttattacaggc                                                                                                                                                                              <  1:791522/223‑22 (MQ=255)                                                                                      gTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCa                                                                                                                                             >  1:288970/1‑251 (MQ=255)                                                                                      gTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCa                                                                                                                                             >  1:405534/1‑251 (MQ=255)                                                                                           aaGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAg                                                                                                                                        >  1:861787/1‑251 (MQ=255)                                                                                                gttTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAgg                                                                                                                                                                                                                                  <  1:390869/156‑1 (MQ=255)                                                                                                gttTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTa                                                                                                                                                             <  1:604861/225‑1 (MQ=255)                                                                                                                           ttaatGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTc                                                                                                                                                                                                                            <  1:827703/135‑1 (MQ=255)                                                                                                                                   gCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctat                                                                                                   >  1:597387/1‑235 (MQ=255)                                                                                                                                     ttATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctatagggg                                                                                              <  1:567090/251‑19 (MQ=255)                                                                                                                                        tatTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCa                                                                                                                                                                                                                           <  1:54676/123‑1 (MQ=255)                                                                                                                                        tatTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGc                                                                                                                                                                                                               >  1:381365/1‑135 (MQ=255)                                                                                                                                        tatTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGc                                                                                                                                                                                                               <  1:803990/135‑1 (MQ=255)                                                                                                                                        tatTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTct                                                                                                                                                                                                  >  1:488837/1‑147 (MQ=255)                                                                                                                                        tatTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCgg                                                                                                                                                               <  1:263360/183‑1 (MQ=255)                                                                                                                                        tatTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctataggggagg                                                                                           >  1:691957/1‑230 (MQ=255)                                                                                                                                        tatTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctataggggagg                                                                                           >  1:281931/1‑230 (MQ=255)                                                                                                                                          ttCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCtaac                                                                                                                                                   <  1:778425/193‑4 (MQ=255)                                                                                                                                            cATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTa                                                                                                                                         <  1:3738/201‑1 (MQ=255)                                                                                                                                            cATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCAt                                                                                                                 <  1:582068/225‑1 (MQ=255)                                                                                                                                            cATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctataggggaggacc                                                                                        >  1:604411/1‑226 (MQ=255)                                                                                                                                            cATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctataggggaggacc                                                                                        >  1:251184/1‑226 (MQ=255)                                                                                                                                            cATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctataggggagg                                                                                           <  1:871446/247‑22 (MQ=255)                                                                                                                                            cATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATa                                                                                                                <  1:109575/226‑1 (MQ=255)                                                                                                                                                      gATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAggcg                                                                                                                                     <  1:142468/195‑3 (MQ=255)                                                                                                                                                      gATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctatagg                                                                                                <  1:1051/232‑17 (MQ=255)                                                                                                                                                      gATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctat                                                                                                   >  1:515490/1‑216 (MQ=255)                                                                                                                                                           gtgtTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctatagggg                                                                                              >  1:499845/1‑211 (MQ=255)                                                                                                                                                           gtgtTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagac                                                                                                             <  1:622235/214‑4 (MQ=255)                                                                                                                                                                               tgttgGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCtaac                                                                                                                                                   >  1:391947/1‑153 (MQ=255)                                                                                                                                                                                         gttttgtttATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAAc                                                                                                                                                                      >  1:375250/1‑127 (MQ=255)                                                                                                                                                                                         gttttgtttATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctatag                                                                                                 >  1:504647/1‑181 (MQ=255)                                                                                                                                                                                         gttttgtttATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGa                                          >  1:816286/1‑251 (MQ=255)                                                                                                                                                                                                          aCTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCaa                                                                                                                                                                                                       <  1:229897/77‑1 (MQ=255)                                                                                                                                                                                                              ggTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAgg                                           <  1:482687/229‑1 (MQ=255)                                                                                                                                                                                                              ggTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGATGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTAt                     >  1:238451/1‑251 (MQ=255)                                                                                                                                                                                                                   gTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTaa                                                                                                                                                            >  1:12792/1‑111 (MQ=255)                                                                                                                                                                                                                   gTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATagacgcctctctatagg                                                                                                <  1:335149/171‑17 (MQ=255)                                                                                                                                                                                                                   gTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAgg                                           <  1:513144/224‑1 (MQ=255)                                                                                                                                                                                                                   gTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAgg                                           >  1:771087/1‑224 (MQ=255)                                                                                                                                                                                                                   gTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGag                                         >  1:617992/1‑225 (MQ=255)                                                                                                                                                                                                                   gTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTa                 >  1:290066/1‑250 (MQ=255)                                                                                                                                                                                                                   gTACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTa                 >  1:579626/1‑250 (MQ=255)                                                                                                                                                                                                                    tACCGTAATAAAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTAc                <  1:271444/250‑1 (MQ=255)                                                                                                                                                                                                                           ataaAAAAACATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAAc                        >  1:549062/1‑235 (MQ=255)                                                                                                                                                                                                                             aaaaaaaCATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCActaattt      <  1:678777/251‑7 (MQ=255)                                                                                                                                                                                                                                 aaaCATTCAACGCAATGTTTCCAGAGGTAATTCAGATTCTCAATGCTGCAGCAACTTCTAGCGTAGGCTTATTACAGGCATTGGAACGTTGCGGTAAGGATCTAACAGGTCAATTAGGCGAGGAGTTCAAAAGTATTCATAGACGCCTCTCTATAGGGGAGGACCCCGCTACAGTTTTTGACGATAGCTATGATCGTTACCCATATAAGGAGTTTTACTTTTTCATAACTATTGTACGCACTAATTTaaac  <  1:351924/251‑3 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                     TTTTTTTAAGAGCCTCAAATCAAAAATTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAGAAACTTGATTCGCAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGATCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAATAAAAAAACATTCAACGCAATGTTTCCGGAGGTAATTCAGATTCTCAATGCAGCGGCAACTTCAGGTGCTGGCTTATTACAAGCATTAGAACGCTGCGGTAAGGATCTTACCGGTCAATTAGGTGAGGAGTTTAAGAGTATCCATAAGCGTATTGCAATAGGCGAGGATCCTGTTTCAGTTTTTGAAGACAGCTATGATCGTTACCCATATAAGGAATTCTATTTCTTCATAACTATTGTACGCACCAACTTAAGT  >  1/1484460‑1484934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑