Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,484,451	A→G	R40R (AGA→AGG)	unknown →	Flp pilus assembly protein TadB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,484,451	0	A	G	100.0%	53.2 / NA	20	R40R (AGA→AGG)	unknown	Flp pilus assembly protein TadB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/8);  total (12/8)

TAAAGAGGGTAAAATTCTTGGTGAGTTTATTAATCACGGACTACTAACTCGTTCTGTTGTATTCCAAAATGCTCAAGTATTCAATCTGAGTGCTGAACTACAAAGTATCTTCGGAGAAGCTGAGTAATGAATTATTATTTGTATTATGCCGTCCTAACAATAGGTGGGCTGTTATTTATATATACCTTAAGTAGTTGGTTTTCCACTAAAAGAAAAATTAATGAGTATGATACCTCTGTAAGTAGAACAACAAGTTTTTTTAAGAGCCTCAAATCAAAAATTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAGAAACTTGATTCGCAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGATCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAA  >  1/1484206‑1484678                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    tAAAGATGGCAAAATTATTGGTGAATTTGTTAACCATGGTTTATTAACTCGCTCTGCTGTTTTCCAAAATGCTCAAGTATTTAATCTGAGTACTGAGTTACAAAGTATTTTCGGAGAAGCTGAGTGATGAGCCATTATTTATATTATGCCGTCCTTACAATAGGTGGACTATTATTTATATATACCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGgacaac                                                                                                                                                                                                                                <  1:538473/251‑1 (MQ=255)                                                                                        gagtactgagttACAAAGTATTTTCGGAGAAGCTGAGTGATGAGCCATTATTTATATTATGCCGTCCTTACAATAGGTGGACTATTATTTATATATACCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAatat                                                                                                                                         >  1:68201/12‑251 (MQ=255)                                                                                           tactgagttACAAAGTATTTTCGGAGAAGCTGAGTGATGAGCCATTATTTATATTATGCCGTCCTTACAATAGGTGGACTATTATTTATATATACCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTg                                                                                                                                       <  1:84604/242‑1 (MQ=255)                                                                                                                       gctgagtgatgagccattattTATATTATGCCGTCCTTACAATAGGTGGACTATTATTTATATATACCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTtata                                                                                                                                  >  1:660140/16‑227 (MQ=255)                                                                                                                            gtgatgagccattattTATATTATGCCGTCCTTACAATAGGTGGACTATTATTTATATATACCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAAc                                                                                                                                                       <  1:289075/191‑1 (MQ=255)                                                                                                                            gtgatgagccattattTATATTATGCCGTCCTTACAATAGGTGGACTATTATTTATATATACCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGt                                                                                                     >  1:795947/11‑251 (MQ=255)                                                                                                                                             atattatGCCGTCCTTACAATAGGTGGACTATTATTTATATATACCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAagaacc                                                                                                                                                                                                               <  1:450250/127‑4 (MQ=255)                                                                                                                                                           ttaCAATAGGTGGACTATTATTTATATATACCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGac                                                                         >  1:414867/3‑247 (MQ=255)                                                                                                                                                                               tttatATATACCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCAc                                                                                                                                                                               >  1:501423/1‑126 (MQ=255)                                                                                                                                                                                    atataCCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGt                                                                                            <  1:299468/204‑1 (MQ=255)                                                                                                                                                                                       taCCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCttgttg                                          <  1:657485/251‑1 (MQ=255)                                                                                                                                                                                        aCCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAgt                                                                                                                               <  1:431997/165‑1 (MQ=255)                                                                                                                                                                                        aCCTTAAGTAGTTGGTTTTCTACTAAAAGAAAAATTAATGAGTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTgg                                         >  1:192908/1‑251 (MQ=255)                                                                                                                                                                                                                                 gTATGATACCTCTATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGt                                                                                            >  1:482514/1‑159 (MQ=255)                                                                                                                                                                                                                                          ctctATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCatat                                                                             <  1:897551/165‑1 (MQ=255)                                                                                                                                                                                                                                          ctctATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAgtgt                                                               <  1:492030/179‑1 (MQ=255)                                                                                                                                                                                                                                          ctctATAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGtaa  >  1:420882/1‑240 (MQ=255)                                                                                                                                                                                                                                           tctatAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTaa                                                                                                                                                                       >  1:99304/5‑74 (MQ=255)                                                                                                                                                                                                                                           tctatAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTc                                                                                 >  1:831896/5‑160 (MQ=255)                                                                                                                                                                                                                                           tctatAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCatat                                                                             >  1:604747/5‑164 (MQ=255)                                                                                                                                                                                                                                            ctatAAGTAGGACAACAAGTTTTTTTAAGAACCTCAAATCAAAAGTTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAAAAACTTAGTTCGTAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGACCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACt                 >  1:726634/4‑223 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    TAAAGAGGGTAAAATTCTTGGTGAGTTTATTAATCACGGACTACTAACTCGTTCTGTTGTATTCCAAAATGCTCAAGTATTCAATCTGAGTGCTGAACTACAAAGTATCTTCGGAGAAGCTGAGTAATGAATTATTATTTGTATTATGCCGTCCTAACAATAGGTGGGCTGTTATTTATATATACCTTAAGTAGTTGGTTTTCCACTAAAAGAAAAATTAATGAGTATGATACCTCTGTAAGTAGAACAACAAGTTTTTTTAAGAGCCTCAAATCAAAAATTAGTTTATGGTATTATCACTTTACTAAAGGTGATAAAAGAAACTTGATTCGCAATATTGTTATAAGTTGTTTTATTTTCTTTGCTTTTTTTGTAATTAATGTTGCTTATATTCATATTGATCGATTAGTGTTTTGCTTAATTTTTCTTGTTGGATTTGTTTTGTTTATTTGGAAACTTGGTCAGTACCGTAA  >  1/1484206‑1484678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑