Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,484,038	A→G	L329L (TTA→TTG)	unknown →	Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,484,038	0	A	G	100.0%	91.1 / NA	34	L329L (TTA→TTG)	unknown	Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/18);  total (16/18)

TGAGCATACAGGCGAAGTTACTATGCAAGACTTAGTAATAAATGCATTGCGTATGCGTCCTGAGCGTATTATTGTGGGCGAATGCCGTGGTGGAGAAGCATTCCAAATGTTACAAGCAATGAATACCGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATAGCCCTCGCGATGCAACATCTCGCTTGGA‑A‑AGTATGGTTATGATGTCGAATGCATCCCTACCGTTAGAGGCAATTCGCCGTAATATCTCTTCAGCAGTAAACATAATAGTGCAGGCGTCCCGTTTAAATGATGGTTCAAGAAAAATTATGAATATCACTGAGGTTATGGGAATGGAAAACGGTCAAATCGTTTTACAGGATATATTTTCCTATAAGGCAAGTAAATATCGTGATAAAGAGGGTAAAATTCTTGGTGAGTTTATTAATCACGGACTACTAAC  >  1/1483810‑1484253                                                                                                                                                                                                                                       |                                                                                                                                                                                                                        tGAGCATACAGGCGAGGTTACTATGCAGGATTTAGTAATAAATGCACTACGTATGCGTCCTGAGCGTATTATCGTAGGTGAATGCCGTGGTGGAGAAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAatattg                                                                                                                                                                                                   <  1:117009/251‑3 (MQ=255)     cATACAGGCGAGGTTACTATGCAGGATTTAGTAATAAATGCACTACGTATGCGTCCTGAGCGTATTATCGTAGGTGAATGCCGTGGTGGAGAAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAatattgca                                                                                                                                                                                                 <  1:292012/249‑5 (MQ=255)            gCGAGGTTACTATGCAGGATTTAGTAATAAATGCACTACGTATGCGTCCTGAGCGTATTATCGTAGGTGAATGCCGTGGTGGAGAAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAatattgcatctgcag                                                                                                                                                                                          <  1:538650/249‑12 (MQ=255)             cgaggTTACTATGCAGGATTTAGTAATAAATGCACTACGTATGCGTCCTGAGCGTATTATCGTAGGTGAATGCCGTGGTGGAGAAGCATTCCAGATGTTACAAGCGATGAACGCTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAatattgcatctgcagtaa                                                                                                                                                                                       <  1:28129/247‑15 (MQ=255)                  ttACTATGCAGGATTTAGTAATAAATGCACTACGTATGCGTCCTGAGCGTATTATCGTAGGTGAATGCCGTGGTGGAGAAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAatattgcatctgcagtaaatatc                                                                                                                                                                                  <  1:689306/251‑20 (MQ=255)                  ttACTATGCAGGATTTAGTAATAAATGCACTACGTATGCGTCCTGAGCGTATTATCGTAGGTGAATGCCGTGGTGGAGAAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAatattgcatctgcagtaaatatc                                                                                                                                                                                  <  1:215324/251‑20 (MQ=255)                                            gcactacGTATGCGTCCTGAGCGTATTATCGTAGGTGAATGCCGTGGTGGAGAAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAatat                                                                                                                                                                                                     <  1:872275/200‑1 (MQ=255)                                               ctacGTATGCGTCCTGAGCGTATTATCGTAGGTGAATGCCGTGGTGGAGAAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAatattgcatctgcagtaaatatc                                                                                                                                                                                  <  1:275003/219‑20 (MQ=255)                                                 acGTATGCGTCCTGAGCGTATTATCGTAGGTGAATGCCGTGGTGGAGAAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAatattgcatctgcagtaaat                                                                                                                                                                                     <  1:743762/216‑17 (MQ=255)                                                 acGTATGCGTCCTGAGCGTATTATCGTAGGTGAATGCCGTGGTGGAGAAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAatattgcatc                                                                                                                                                                                               <  1:659428/206‑7 (MQ=255)                                                 acGTATGCGTCCTGAGCGTATTATCGTAGGTGAATGCCGTGGTGGAGAAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAatat                                                                                                                                                                                                     >  1:776148/2‑201 (MQ=255)                                                            cTGAGCGTATTATCGTAGGTGAATGCCGTGGTGGAGAAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAAtc                                                                                                                                        <  1:281034/251‑2 (MQ=255)                                                             tGAGCGTATTATCGTAGGTGAATGCCGTGGTGGAGAAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAAtca                                                                                                                                       <  1:782966/251‑3 (MQ=255)                                                                      tatcgtaggtgAATGCCGTGGTGGAGAAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAatatta                                                                                                                              >  1:405504/11‑249 (MQ=255)                                                                       atcgtaggtgAATGCCGTGGTGGAGAAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGAGAGAGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAatattg                                                                                                                                                                                                   >  1:807965/10‑181 (MQ=255)                                                                       atcgtaggtgAATGCCGTGGTGGAGAAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAatattac                                                                                                                             <  1:563338/242‑4 (MQ=255)                                                                       atcgtaggtgAATGCCGTGGTGGAGAAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAatatta                                                                                                                              >  1:418153/10‑248 (MQ=255)                                                                       atcgtaggtgAATGCCGTGGTGGAGAAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAatatta                                                                                                                              >  1:629222/10‑248 (MQ=255)                                                                                           tggAGAAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAATATTACTGAAATAATGGGTATGGaaa                                                                                                         <  1:462289/251‑1 (MQ=255)                                                                                           tggAGAAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAATATTACTGAAATAATGGGTATGGaa                                                                                                          <  1:676093/250‑1 (MQ=255)                                                                                               gaAGCATTCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAATATTACTGAAATAATGGGTATGGaaaatgg                                                                                                     >  1:390491/1‑248 (MQ=255)                                                                                                     ttCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAatattgcatctgcagtaaat                                                                                                                                                                                     <  1:316392/165‑17 (MQ=255)                                                                                                     ttCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAatattac                                                                                                                             >  1:846452/1‑218 (MQ=255)                                                                                                     ttCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAATATTACTGAAATAATGGGTATGGAAAATGGGCAAAt                                                                                               >  1:145787/1‑251 (MQ=255)                                                                                                     ttCCAGATGTTACAAGCGATGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAATATTACTGAAATAATGGGTATGGAAAATGGGCAAAt                                                                                               >  1:775030/1‑251 (MQ=255)                                                                                                                    gcgaTGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAatat                                                                                                                                                                                                     >  1:12367/4‑134 (MQ=255)                                                                                                                     cgaTGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAatattgcat                                                                                                                                                                                                <  1:30257/136‑6 (MQ=255)                                                                                                                      gaTGAACACTGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAATATTACTGaaataatgggtatgga                                                                                                           >  1:737220/2‑207 (MQ=255)                                                                                                                          aacactggTCACGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAATATTACTGAAATAATGGGTATGGAAAATGGGCAAATTGTTTTACAAGATATTTTTTc                                                                          <  1:401941/245‑1 (MQ=255)                                                                                                                                     cGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAATATTACTGAAATAATGGGTATGGAAAATGGGCAAATTGTTTTACAAGATATTTTTTCTTATAAGGCaa                                                               >  1:833597/1‑251 (MQ=255)                                                                                                                                     cGATGGTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAATATTACTGAAATAATGGGTATGGAAAATGGGCAAATTGTTTTACAAGATATTTTTTCTTATAAGGCaa                                                               >  1:105586/1‑251 (MQ=255)                                                                                                                                         ggTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAGAATCATGAATATTACTGAAATAATGGGTATGGAAAATGGGCAAATTGTTTTACAAGATATTTTTTCTTATAAGGCAAGTaa                                                           >  1:438528/1‑251 (MQ=255)                                                                                                                                         ggTTCTATGTCAACGTTGCACGCAAATACCCCTCGGGATGCAACATCTCGATTGGA‑G‑AGTATGGTTATGATGTCGAATGCGTCCCTACCGTTGGAAGCAATTCGCCGTAATATTGCATCTGCAGTAAATATCATCGTACAAGCCTCCCGTTTAAATGATGGAACAAGAAAAATCATGAATATTACTGAAATAATGGGTATGGAAAATGGGCAAATTGTTTTACAAGATATTTTTTCTTATAAGGCAAGTaa                                                           >  1:323313/1‑251 (MQ=255)                                                                                                                                                                                                         ggTTATGATGTCGAATGCATCCCTACCGTTGGAGGCAATTCGCCGTAATATTTCTTCAGCAGTAAACATAATAGTGCAGGCGTCCCGCTTAAACGATGGTTCAAGAAAAATTATGAATATCACTGAAGTTATGGGAATGGAAAACGGTCAAATCGTTTTGCAAGATATATTTTCCTATAAGGCAAGTAAATATCGTGATAAAGAGGGTAAAATTCTTGGCGAGTTTATTAATCACGGACTACTAAc  >  1:507049/1‑246 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                        TGAGCATACAGGCGAAGTTACTATGCAAGACTTAGTAATAAATGCATTGCGTATGCGTCCTGAGCGTATTATTGTGGGCGAATGCCGTGGTGGAGAAGCATTCCAAATGTTACAAGCAATGAATACCGGTCACGATGGTTCTATGTCAACGTTGCACGCAAATAGCCCTCGCGATGCAACATCTCGCTTGGA‑A‑AGTATGGTTATGATGTCGAATGCATCCCTACCGTTAGAGGCAATTCGCCGTAATATCTCTTCAGCAGTAAACATAATAGTGCAGGCGTCCCGTTTAAATGATGGTTCAAGAAAAATTATGAATATCACTGAGGTTATGGGAATGGAAAACGGTCAAATCGTTTTACAGGATATATTTTCCTATAAGGCAAGTAAATATCGTGATAAAGAGGGTAAAATTCTTGGTGAGTTTATTAATCACGGACTACTAAC  >  1/1483810‑1484253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑