Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 254,513 A→C L101V (TTG→GTG)  unknown ← Acyl‑CoA:1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase (EC 2.3.1.51) @ Acyl‑ACP:1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase (EC 2.3.1.n4)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1254,5130AC98.8% 231.1 / NA 85L101V (TTG→GTG) unknownAcyl‑CoA:1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase (EC 2.3.1.51) @ Acyl‑ACP:1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase (EC 2.3.1.n4)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (50/34);  minor base T (0/1);  total (50/35)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTAAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCAACCAAATCAGGCTTTTCTTACCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGCCGACCGAACCAACGGGCGAAAATGCCCACGTTACTTGGGTTACGAAAACGAATGAAAGAATAGAGCGTGCCGAGGA  >  1/254267‑254758
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     
gtgtAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCaa                                                                                                                                                                                                                                                   >  1:722549/1‑251 (MQ=255)
   tAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAAt                                                                                                                                                                                                                                                 >  1:353316/1‑250 (MQ=255)
        gAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAgg                                                                                                                                                                                                                                             >  1:179695/1‑249 (MQ=255)
             gACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCtttt                                                                                                                                                                                                                                        >  1:396207/1‑249 (MQ=255)
                  ccGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCg                                                                                                                                                                                                                                 >  1:548456/1‑251 (MQ=255)
                          aCAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGa                                                                                                                                                                                                                          >  1:849291/1‑250 (MQ=255)
                                           aaTGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCa                                                                                                                                                                                                        <  1:467023/251‑1 (MQ=255)
                                                cagcaTGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTGCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCAtata                                                                                                                                                                                                    <  1:228159/250‑1 (MQ=255)
                                                  gcaTGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg                                                                                                                                                                                                  >  1:739742/1‑250 (MQ=255)
                                                   caTGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg                                                                                                                                                                                                  <  1:498474/249‑1 (MQ=255)
                                                   caTGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg                                                                                                                                                                                                  <  1:428339/249‑1 (MQ=255)
                                                   caTGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg                                                                                                                                                                                                  <  1:879467/249‑1 (MQ=255)
                                                   caTGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg                                                                                                                                                                                                  <  1:456852/249‑1 (MQ=255)
                                                   caTGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg                                                                                                                                                                                                  <  1:734407/249‑1 (MQ=255)
                                                   caTGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGaa                                                                                                                                                                                                >  1:739821/1‑251 (MQ=255)
                                                   caTGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGaa                                                                                                                                                                                                >  1:92976/1‑251 (MQ=255)
                                                                 gTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg                                                                                                                                                                                                  >  1:684943/1‑235 (MQ=255)
                                                                           gCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTAttt                                                                                                                                                                        >  1:651875/1‑251 (MQ=255)
                                                                           gCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTAttt                                                                                                                                                                        >  1:773646/1‑251 (MQ=255)
                                                                           gCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTAttt                                                                                                                                                                        >  1:414028/1‑251 (MQ=255)
                                                                                       gtccgcgACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCg                                                                                                                                                            >  1:471727/1‑251 (MQ=255)
                                                                                       gtccgcgACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCg                                                                                                                                                            >  1:397208/1‑251 (MQ=255)
                                                                                              aCTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATaaa                                                                                                                                                     >  1:109061/1‑251 (MQ=255)
                                                                                               cTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAAt                                                                                                                                                    <  1:826947/251‑1 (MQ=255)
                                                                                                tGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATg                                                                                                                                                   <  1:495736/251‑1 (MQ=255)
                                                                                                  cGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGc                                                                                                                                                  <  1:523484/250‑1 (MQ=255)
                                                                                                        ccTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACgg                                                                                                                                                                                                                       <  1:864830/175‑1 (MQ=255)
                                                                                                         cTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAAGATAAAATGCCGAAAAACGGCGCCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg                                                                                                                                                                                                  <  1:504947/195‑1 (MQ=255)
                                                                                                                   cATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg                                                                                                                                                                                                  >  1:764423/1‑185 (MQ=255)
                                                                                                                       cAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACgg                                                                                                                                                                                                                       >  1:747804/1‑160 (MQ=255)
                                                                                                                       cAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATgg                                                                                                                                                                                            <  1:87218/187‑1 (MQ=255)
                                                                                                                       cAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTAtt                                                                                                                            >  1:388848/1‑251 (MQ=255)
                                                                                                                         aaTAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTcg                                                                                                                          >  1:751266/1‑251 (MQ=255)
                                                                                                                           tAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg                                                                                                                                                                                                  >  1:780696/1‑177 (MQ=255)
                                                                                                                           tAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGc                                                                                                                                                  <  1:43209/225‑1 (MQ=255)
                                                                                                                                     gTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATg                                                                                                                                                   <  1:166830/214‑1 (MQ=255)
                                                                                                                                     gTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTcggc                                                                                                                        >  1:558646/1‑241 (MQ=255)
                                                                                                                                     gTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGa                                                                                                               >  1:722967/1‑250 (MQ=255)
                                                                                                                                     gTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGAt                                                                                                              >  1:42003/1‑251 (MQ=255)
                                                                                                                                      tCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATg                                                                                                             <  1:52494/251‑1 (MQ=255)
                                                                                                                                          gATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCActat                                                                                                                                                                                     <  1:506506/175‑4 (MQ=255)
                                                                                                                                          gATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCAt                                                                                                                                                                                     <  1:506540/175‑1 (MQ=255)
                                                                                                                                          gATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGtttg                                                                                                         >  1:291232/1‑249 (MQ=255)
                                                                                                                                          gATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCg                                                                                                         >  1:58934/1‑251 (MQ=255)
                                                                                                                                          gATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCg                                                                                                         >  1:657128/1‑251 (MQ=255)
                                                                                                                                           aTTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGa                                                                                                        <  1:372469/251‑1 (MQ=255)
                                                                                                                                            tttATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGa                                                                                                        >  1:438610/1‑250 (MQ=255)
                                                                                                                                                  cggcgTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAg                                                                                                 >  1:871359/1‑251 (MQ=255)
                                                                                                                                                       tGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGa                                                                                            >  1:93184/1‑251 (MQ=255)
                                                                                                                                                             ttCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAg                                                                                        <  1:123677/249‑1 (MQ=255)
                                                                                                                                                             ttCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCg                                                                                      >  1:802677/1‑251 (MQ=255)
                                                                                                                                                               ccGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGa                                                                                    >  1:652889/1‑251 (MQ=255)
                                                                                                                                                                 gTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg                                                                                                                                                                                                  <  1:292386/139‑1 (MQ=255)
                                                                                                                                                                 gTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGt                                                                                                                                                                                >  1:741619/1‑157 (MQ=255)
                                                                                                                                                                       gtgtTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg                                                                                                                                                                                                  >  1:708189/1‑133 (MQ=255)
                                                                                                                                                                         gttgtGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg                                                                                                                                                                                                  >  1:496534/1‑131 (MQ=255)
                                                                                                                                                                         gttgtGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTc                                                                                                                           >  1:85280/1‑202 (MQ=255)
                                                                                                                                                                         gttgtGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGccga                                                                           >  1:414637/1‑250 (MQ=255)
                                                                                                                                                                         gttgtGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGccga                                                                           >  1:367432/1‑250 (MQ=255)
                                                                                                                                                                           tgtgGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCG‑AAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGGAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGc                                                                              >  1:244221/1‑244 (MQ=255)
                                                                                                                                                                                gCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGCCGACCGAACCa                                                                   >  1:305430/1‑251 (MQ=255)
                                                                                                                                                                                         cGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTc                                                                                                          >  1:43698/1‑203 (MQ=255)
                                                                                                                                                                                                    aTCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACTATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGCCGACCGAACCAACGGGCGAAAATGCCTACGt                                               <  1:481112/251‑1 (MQ=255)
                                                                                                                                                                                                     tCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGCCGACCGAACCAACGGGCGAAAATGCCTACGtt                                              <  1:263417/251‑1 (MQ=255)
                                                                                                                                                                                                            aaaTATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGCCGACCGAACCAACGGGCGAAAATGCCTACGTTACTTggg                                       <  1:28983/251‑1 (MQ=255)
                                                                                                                                                                                                              atatTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCAcc                                                                                                                                                                                       >  1:518331/1‑105 (MQ=255)
                                                                                                                                                                                                                  tGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGCCGACCGAACCAACGGGCGAAAATGCCTACGTTACTTGGGTTACGa                                 <  1:672554/251‑1 (MQ=255)
                                                                                                                                                                                                                   gCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTAttt                                                                                                                                                                        >  1:235141/1‑115 (MQ=255)
                                                                                                                                                                                                                    cccGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGCCGACCGAAcc                                                                    >  1:488461/1‑214 (MQ=255)
                                                                                                                                                                                                                    cccGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGCCGACCGAACCAACGGGCGAAAATGCCTACGTTACTTGGGTTACGaa                                <  1:745184/250‑1 (MQ=255)
                                                                                                                                                                                                                    cccGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGCCGACCGAACCAACGGGCGAAAATGCCTACGTTACTTGGGTTACGaa                                <  1:569776/250‑1 (MQ=255)
                                                                                                                                                                                                                          gCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGAc                                                                                                                                                                                                                         <  1:249191/59‑1 (MQ=255)
                                                                                                                                                                                                                          gCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg                                                                                                                                                                                                  >  1:258816/1‑82 (MQ=255)
                                                                                                                                                                                                                               atatAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg                                                                                                                                                                                                  >  1:728451/1‑77 (MQ=255)
                                                                                                                                                                                                                               atatAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGccgacc                                                                         <  1:833154/198‑1 (MQ=255)
                                                                                                                                                                                                                               atatAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGCCGACCGAACCAACGGGCGAAAATGCCTACGtt                                              <  1:362930/225‑1 (MQ=255)
                                                                                                                                                                                                                               atatAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGCCGACCGAACCAACGGGCGAAAATGCCTACGTTACTTGGGTTACGAAAACGAATGAAAg                    <  1:75366/251‑1 (MQ=255)
                                                                                                                                                                                                                               atatAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGCCGACCGAACCAACGGGCGAAAATGCCTACGTTACTTGGGTTACGAAAACGAATGAAAg                    >  1:600879/1‑251 (MQ=255)
                                                                                                                                                                                                                               atatAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGCCGACCGAACCAACGGGCGAAAATGCCTACGTTACTTGGGTTACGAAAACGAATGAAAg                    >  1:199829/1‑251 (MQ=255)
                                                                                                                                                                                                                               atatAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGCCGACCGAACCAACGGGCGAAAATGCCTACGTTACTTGGGTTACGAAAACGAATGAAAg                    >  1:168821/1‑251 (MQ=255)
                                                                                                                                                                                                                                 ataAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGCCGACCGAACCAACGGGCGAAAATGCCTACGTTACTTGGGTTACGAAAACGAATGAAAGaa                  >  1:113016/1‑251 (MQ=255)
                                                                                                                                                                                                                                       tGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGCCGACCGAACCAACGGGCGAAAATGCCTACGTTACTTGGGTTACGAAAACGAATGAAAGAATAGAGc            <  1:264025/251‑1 (MQ=255)
                                                                                                                                                                                                                                       tGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGCCGACCGAACCAACGGGCGAAAATGCCTACGTTACTTGGGTTACGAAAACGAATGAAAGAATAGAGc            <  1:471554/251‑1 (MQ=255)
                                                                                                                                                                                                                                       tGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGCCGACCGAACCAACGGGCGAAAATGCCTACGTTACTTGGGTTACGAAAACGAATGAAAGAATAGAGc            <  1:224355/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 cGGCATCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGCCGACCGAACCAACGGGCGAAAATGCCTACGTTACTTGGGTTACGAAAACGAATGAAAGAATAGAGCGTGCCGAGGa  <  1:298871/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     
GTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTAAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCAACCAAATCAGGCTTTTCTTACCGACGCTGACGGTGCGCGGCATCACCATATAAGAAATGGTCACCATGTCGTAGTTATTTTGGTGGTTGCCGATATAAATGCAGCGCCCGATTTTGTCGGTATTCGGCGGGTAGCGATGTTCGACGTTCAGCCCGAAAAGCGGATATAGCCGACCGAACCAACGGGCGAAAATGCCCACGTTACTTGGGTTACGAAAACGAATGAAAGAATAGAGCGTGCCGAGGA  >  1/254267‑254758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑