Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,483,557	A→G	N169S (AAT→AGT)	unknown →	Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,483,557	0	A	G	100.0%	250.0 / NA	87	N169S (AAT→AGT)	unknown	Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/54);  total (33/54)

TTAATGGCCCTAATGACATCTGGGTTGAACGTGCTGGTATTCTGGAAAAAACTGATAAAGAATTTGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGCCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCTGATGGCAGCCGTTTAAATGTTGTTATTGCACCGATTGCATTAGATGGCACATCCATTTCTATTCGTAAATTCAGTAAGAATAAAAAAACATTGCAAGAGCTTGTAAATTTTGGTTCTATGACTCGCGAAATGGCGAATTTCTTAATTATTGCTGCCAGATCCCGGGTAAATATAATTGTGTCAGGTGGTACAGGATCTGGTAAAACAACCTTACTTAATGCGCTATCTAACTATATTTCCCATACCGAACGTGTCATCACACTAGAAGATACTGCCGAGCTGCGTTTGGAACAGCCTCATGTTGTACGCCTGGAAACG  >  1/1483314‑1483795                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               ttAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTaaaaaa                                                                                                                                                                                                                                         <  1:834553/251‑1 (MQ=255) ttAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTaaaaaa                                                                                                                                                                                                                                         <  1:286497/251‑1 (MQ=255) ttAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTaaaaaa                                                                                                                                                                                                                                         >  1:247948/1‑251 (MQ=255) ttAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTaaaaa                                                                                                                                                                                                                                          <  1:170150/250‑1 (MQ=255)        cccTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTaaaaaaagcttg                                                                                                                                                                                                                                   >  1:813685/1‑245 (MQ=255)           tAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAag                                                                                                                                                                                                                               <  1:136815/251‑1 (MQ=255)                 taTCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAag                                                                                                                                                                                                                               <  1:557463/244‑1 (MQ=255)                 taTCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTg                                                                                                                                                                                                                         >  1:818981/2‑251 (MQ=255)                                                                 cgTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAattatcg                                                                                                                                                                         >  1:374876/2‑249 (MQ=255)                                                                      gTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATcgctgc                                                                                                                                                                     >  1:445950/1‑250 (MQ=255)                                                                             aCAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATct                                                                                                                                                              >  1:538625/1‑249 (MQ=255)                                                                              cAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTaaaaa                                                                                                                                                                                                                                          <  1:53035/173‑1 (MQ=255)                                                                                aTTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATctcgag                                                                                                                                                          <  1:593720/251‑6 (MQ=255)                                                                                aTTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATctcgag                                                                                                                                                          >  1:104960/1‑246 (MQ=255)                                                                                   aaCTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAAtt                                                                                                                                                                                                                   >  1:603678/1‑191 (MQ=255)                                                                                   aaCTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGcc                                                                                                                                                                    <  1:105483/238‑1 (MQ=255)                                                                                   aaCTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTaa                                                                                                                                                       >  1:150133/1‑251 (MQ=255)                                                                                          atTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAAt                                                                                                                                                <  1:422609/251‑1 (MQ=255)                                                                                            tGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATtg                                                                                                                                              <  1:211482/251‑1 (MQ=255)                                                                                            tGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATtg                                                                                                                                              <  1:594926/251‑1 (MQ=255)                                                                                             gCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATtgt                                                                                                                                             >  1:501982/1‑251 (MQ=255)                                                                                               aaaaaGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTgtgt                                                                                                                                           <  1:457632/251‑1 (MQ=255)                                                                                                     gTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAggt                                                                                                                                      <  1:597092/250‑1 (MQ=255)                                                                                                     gTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAggt                                                                                                                                      <  1:171629/250‑1 (MQ=255)                                                                                                           tGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAg                                                                                                                               <  1:550899/251‑1 (MQ=255)                                                                                                           tGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAg                                                                                                                               <  1:401498/251‑1 (MQ=255)                                                                                                              tCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGAt                                                                                                                            <  1:854231/251‑1 (MQ=255)                                                                                                              tCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGAt                                                                                                                            <  1:17210/251‑1 (MQ=255)                                                                                                                 ggTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAatat                                                                                                                                                   <  1:132605/225‑1 (MQ=255)                                                                                                                        cgtcGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAaac                                                                                                                  <  1:180538/248‑1 (MQ=255)                                                                                                                        cgtcGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAaac                                                                                                                  <  1:618678/248‑1 (MQ=255)                                                                                                                          tcGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGAt                                                                                                                                                                <  1:898626/202‑1 (MQ=255)                                                                                                                          tcGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAaacaa                                                                                                                >  1:323857/2‑251 (MQ=255)                                                                                                                            gTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAaacaa                                                                                                                >  1:273647/1‑249 (MQ=255)                                                                                                                                   tgatgGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGAt                                                                                                                            <  1:272976/230‑1 (MQ=255)                                                                                                                                      tgGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAaacaactctccttaatg                                                                                                    >  1:519038/1‑240 (MQ=255)                                                                                                                                      tgGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAaacaactctccttaatg                                                                                                    <  1:814888/251‑12 (MQ=255)                                                                                                                                      tgGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAaacaactctccttaatg                                                                                                    <  1:819003/251‑12 (MQ=255)                                                                                                                                        gTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATgc                                                                                                   <  1:635676/250‑1 (MQ=255)                                                                                                                                           gTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTa                                                                                               >  1:40588/1‑251 (MQ=255)                                                                                                                                           gTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTa                                                                                               >  1:601913/1‑251 (MQ=255)                                                                                                                                                   gTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTaatta                                                                                       >  1:408684/1‑248 (MQ=255)                                                                                                                                                    tGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTTGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTaatta                                                                                       <  1:448071/250‑4 (MQ=255)                                                                                                                                                    tGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTaatta                                                                                       <  1:697782/250‑4 (MQ=255)                                                                                                                                                    tGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTaatta                                                                                       <  1:422294/250‑4 (MQ=255)                                                                                                                                                      gACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATtata                                                                                     <  1:2897/250‑1 (MQ=255)                                                                                                                                                             gACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCAt                                                                             >  1:249121/1‑251 (MQ=255)                                                                                                                                                               cTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATAc                                                                           <  1:235047/251‑1 (MQ=255)                                                                                                                                                                 acccgATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTaatta                                                                                       >  1:640523/5‑234 (MQ=255)                                                                                                                                                                  cccgATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGa                                                                        >  1:761072/4‑251 (MQ=255)                                                                                                                                                                    cgATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAAc                                                                      <  1:268674/250‑1 (MQ=255)                                                                                                                                                                     gATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCt                                                                                            >  1:50244/1‑228 (MQ=255)                                                                                                                                                                      aTGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAaacaactctcctt                                                                                                        <  1:89988/215‑8 (MQ=255)                                                                                                                                                                              cGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAACGTGTCATcac                                                            <  1:418083/251‑1 (MQ=255)                                                                                                                                                                               gTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAGAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGAt                                                                                                                            >  1:342199/1‑186 (MQ=255)                                                                                                                                                                                tttAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAACGTGTCATcaca                                                           <  1:516466/250‑1 (MQ=255)                                                                                                                                                                                      tgttgtAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAACGTGTCATCACATTagaag                                                    <  1:489297/251‑1 (MQ=255)                                                                                                                                                                                        ttgtaATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTaatta                                                                                       <  1:571432/214‑4 (MQ=255)                                                                                                                                                                                        ttgtaATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAACGTGTCATCACATTAGAAGAt                                                  <  1:544612/251‑1 (MQ=255)                                                                                                                                                                                          gtaaTTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATgc                                                                                                   <  1:619802/197‑1 (MQ=255)                                                                                                                                                                                          gtaaTTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCt                                                                                            >  1:731078/4‑207 (MQ=255)                                                                                                                                                                                           taaTTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGAt                                                                                                                            <  1:857935/172‑1 (MQ=255)                                                                                                                                                                                           taaTTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAaacaactctcctt                                                                                                        <  1:479978/192‑8 (MQ=255)                                                                                                                                                                                           taaTTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAaacaactc                                                                                                             <  1:616421/187‑3 (MQ=255)                                                                                                                                                                                           taaTTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAACGTGTCATCACATTAGAAGATAc                                                <  1:401642/248‑1 (MQ=255)                                                                                                                                                                                           taaTTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAACGTGTCATCACATTAGAAGATAc                                                <  1:521795/248‑1 (MQ=255)                                                                                                                                                                                                         gcgttAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGAt                                                                                                                            <  1:303931/157‑1 (MQ=255)                                                                                                                                                                                                         gcgttAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAACGTGTCATCACATTagaag                                                    >  1:595869/4‑232 (MQ=255)                                                                                                                                                                                                         gcgttAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAACGTGTCATCACATTAGAAGATACTGCTGAATTGCGttt                                 >  1:437599/4‑251 (MQ=255)                                                                                                                                                                                                           gttAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAGCTCTCCTTAATGCGCTATCTaatta                                                                                       >  1:429363/2‑192 (MQ=255)                                                                                                                                                                                                              aGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAACGTGTCATCACATTAGAAGATACTGCTGAATTGCGTTTGGaa                             >  1:883240/1‑250 (MQ=255)                                                                                                                                                                                                                aTGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATc                                                                                                                           <  1:56908/154‑1 (MQ=255)                                                                                                                                                                                                                aTGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAACGTGTCATCACATTAGAAGATACTGCTGAATTGCGTTTGGAAc                            <  1:321550/249‑1 (MQ=255)                                                                                                                                                                                                                aTGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAACGTGTCATCACATTAGAAGATACTGCTGAATTGCGTTTGGAACaa                          >  1:144450/1‑250 (MQ=255)                                                                                                                                                                                                                 tggtacaTCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAACGTGTCATCACATTAGAAGATACTGCTGAATTGCGTTTGGAAc                            >  1:652590/5‑248 (MQ=255)                                                                                                                                                                                                                 tggtacaTCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTAAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAACGTGTCATCACATTAGAAGATACTGCTGAATTGCGTTTGGa                              >  1:333926/5‑246 (MQ=255)                                                                                                                                                                                                                   gtacaTCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGAt                                                                                                                            <  1:795901/148‑1 (MQ=255)                                                                                                                                                                                                                   gtacaTCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAACGTGTCATCACATTAGAAGATACTGCTGAATTGCGTTTGGAACaac                         >  1:277005/3‑247 (MQ=255)                                                                                                                                                                                                                       aTCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATAttt                                                                                  >  1:377932/1‑188 (MQ=255)                                                                                                                                                                                                                       aTCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAAc                                                                      >  1:49684/1‑200 (MQ=255)                                                                                                                                                                                                                        tccatttccattCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAACGTGTCATCACATTAGAAGATACTGCTGAATTGCGTTTGGAACAACCTCAtgt                  <  1:290863/251‑1 (MQ=255)                                                                                                                                                                                                                        tccatttccattCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAACGTGTCATCACATTAGAAGATACTGCTGAATTGCGTTTGGAACAACCTCAtgt                  <  1:843206/251‑1 (MQ=255)                                                                                                                                                                                                                        tccatttccattCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAACGTGTCATCACATTAGAAGATACTGCTGAATTGCGTTTGGAACAACCTCAtgt                  <  1:450213/251‑1 (MQ=255)                                                                                                                                                                                                                        tccatttccattCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAACGTGTCATCACATTAGAAGATACTGCTGAATTGCGTTTGGAACAACCTCAtgt                  <  1:207061/251‑1 (MQ=255)                                                                                                                                                                                                                               ccaTTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAACGTGTCATCACATTAGAAGATACTGCTGAATTGCGTTTGGAACAACCTCATGTTGTACGc           <  1:508393/249‑1 (MQ=255)                                                                                                                                                                                                                                         gttCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATgc                                                                                                   <  1:340412/152‑1 (MQ=255)                                                                                                                                                                                                                                         gttCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATACCGAACGTGTCATCACATTAGAAGATACTGCTGAATTGCGTTTGGAACAACCTCATGTTGTACGCCTTGAAAca  >  1:384333/2‑249 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               TTAATGGCCCTAATGACATCTGGGTTGAACGTGCTGGTATTCTGGAAAAAACTGATAAAGAATTTGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGCCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCTGATGGCAGCCGTTTAAATGTTGTTATTGCACCGATTGCATTAGATGGCACATCCATTTCTATTCGTAAATTCAGTAAGAATAAAAAAACATTGCAAGAGCTTGTAAATTTTGGTTCTATGACTCGCGAAATGGCGAATTTCTTAATTATTGCTGCCAGATCCCGGGTAAATATAATTGTGTCAGGTGGTACAGGATCTGGTAAAACAACCTTACTTAATGCGCTATCTAACTATATTTCCCATACCGAACGTGTCATCACACTAGAAGATACTGCCGAGCTGCGTTTGGAACAGCCTCATGTTGTACGCCTGGAAACG  >  1/1483314‑1483795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑