Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	254,337	A→C	F159L (TTT→TTG)	unknown ←	Acyl‑CoA:1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase (EC 2.3.1.51) @ Acyl‑ACP:1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase (EC 2.3.1.n4)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	254,337	0	A	C	100.0%	161.3 / NA	57	F159L (TTT→TTG)	unknown	Acyl‑CoA:1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase (EC 2.3.1.51) @ Acyl‑ACP:1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase (EC 2.3.1.n4)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (43/14);  total (43/14)

GGATTGGGCAATTTCCTGATCTAATTCATTGATGCGTTTTTGCATTATATCGTGGCAATATTCCGCCAGTTCGCGCACGTTTTCACGGGTATAGCCGGAAACGTCAATGGGTTCCAGCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTAAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCAACCAAATCAGGCTTTTCTTACCGACGCTGACGGTGCGCGGCATCACCATATAAGAA  >  1/254097‑254568                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                        ggATTGGGCAATTTCCTGATCTAATTCATTGATGCGTTTTTGCATTATATCGTGGCAATATTCCGCCAGTTCGCGCACGTTTTCACGGGTATAGCCGGAAACGTCAATGGGTTCCAGCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCaata                                                                                                                                                                                                                               >  1:112172/1‑251 (MQ=255)    ttGGGCAATTTCCTGATCTAATTCATTGATGCGTTTTTGCATTATATCGTGGCAATATTCCGCCAGTTCGCGCACGTTTTCACGGGTATAGCCGGAAACGTCAATGGGTTCCAGCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCGCGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAAtc                                                                                                                                                                                                                            <  1:550705/251‑1 (MQ=255)             ttCCTGATCTAATTCATTGATGCGTTTTTGCATTATATCGTGGCAATATTCCGCCAGTTCGCGCACGTTTTCACGGGTATAGCCGGAAACGTCAATGGGTTCCAGCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAAtccgcgtccg                                                                                                                                                                                                                    >  1:374046/1‑250 (MQ=255)                   aTCTAATTCATTGATGCGTTTTTGCATTATATCGTGGCAATATTCCGCCAGTTCGCGCACGTTTTCACGGGTATAGCCGGAAACGTCAATGGGTTCCAGCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTg                                                                                                                                                                                                              >  1:378506/1‑250 (MQ=255)                                     tttttGCATTATATCGTGGCAATATTCCGCCAGTTCGCGCACGTTTTCACGGGTATAGCCGGAAACGTCAATGGGTTCCAGCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACa                                                                                                                                                                                           >  1:492033/1‑251 (MQ=255)                                          gCATTATATCGTGGCAATATTCCGCCAGTTCGCGCACGTTTTCACGGGTATAGCCGGAAACGTCAATGGGTTCCAGCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCaa                                                                                                                                                                                      >  1:835440/1‑251 (MQ=255)                                          gCATTATATCGTGGCAATATTCCGCCAGTTCGCGCACGTTTTCACGGGTATAGCCGGAAACGTCAATGGGTTCCAGCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCaa                                                                                                                                                                                      >  1:555500/1‑251 (MQ=255)                                           cATTATATCGTGGCAATATTCCGCCAGTTCGCGCACGTTTTCACGGGTATAGCCGGAAACGTCAATGGGTTCCAGCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCaaa                                                                                                                                                                                     >  1:635891/1‑251 (MQ=255)                                                    gTGGCAATATTCCGCCAGTTCGCGCACGTTTTCACGGGTATAGCCGGAAACGTCAATGGGTTCCAGCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAAtc                                                                                                                                                                                                                            >  1:545753/1‑203 (MQ=255)                                                       gCAATATTCCGCCAGTTCGCGCACGTTTTCACGGGTATAGCCGGAAACGTCAATGGGTTCCAGCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGt                                                                                                                                                                         >  1:258244/1‑251 (MQ=255)                                                       gCAATATTCCGCCAGTTCGCGCACGTTTTCACGGGTATAGCCGGAAACGTCAATGGGTTCCAGCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGCGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGt                                                                                                                                                                         >  1:599276/1‑251 (MQ=255)                                                                 gCCAGTTCGCGCACGTTTTCACGGGTATAGCCGGAAACGTCAATGGGTTCCAGCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTAt                                                                                                                                                               >  1:664309/1‑251 (MQ=255)                                                                                        ggTATAGCCGGAAACGTCAATGGGTTCCAGCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATgg                                                                                                                                        >  1:189987/1‑251 (MQ=255)                                                                                         gTATAGCCGGAAACGTCAATGGGTTCCAGCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGgt                                                                                                                                       >  1:555140/1‑251 (MQ=255)                                                                                                              ggTTCCAGCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGAtt                                                                                                                  >  1:51782/1‑251 (MQ=255)                                                                                                               gTTCCAGCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTg                                                                                                                 >  1:560240/1‑251 (MQ=255)                                                                                                               gTTCCAGCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTg                                                                                                                 >  1:581068/1‑251 (MQ=255)                                                                                                               gTTCCAGCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTg                                                                                                                 >  1:883620/1‑251 (MQ=255)                                                                                                               gTTCCAGCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTg                                                                                                                 >  1:476439/1‑251 (MQ=255)                                                                                                                     gCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGAtt                                                                                                                                                                  >  1:778604/1‑196 (MQ=255)                                                                                                                     gCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGAtt                                                                                                                                                                  >  1:130278/1‑196 (MQ=255)                                                                                                                                 tCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGaaa                                                                                                   >  1:551324/1‑247 (MQ=255)                                                                                                                                   aCCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAAtc                                                                                                                                                                                                                            >  1:204622/1‑124 (MQ=255)                                                                                                                                   aCCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATAtt                                                                                             >  1:895374/1‑251 (MQ=255)                                                                                                                                    ccTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATgc                                                                                                                                                             <  1:148650/186‑1 (MQ=255)                                                                                                                                        gCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCAt                                                                                                                                          <  1:524841/201‑1 (MQ=255)                                                                                                                                                       gTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCggg                                                                                                                                                                                               >  1:686976/1‑133 (MQ=255)                                                                                                                                                       gTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATgc                                                                                                                                                             >  1:203623/1‑167 (MQ=255)                                                                                                                                                        tttAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAAt                                                                        <  1:693468/251‑1 (MQ=255)                                                                                                                                                        tttAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAAt                                                                        <  1:590879/251‑1 (MQ=255)                                                                                                                                                                gATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGAtt                                                                                                                                                                  >  1:318766/1‑153 (MQ=255)                                                                                                                                                                 aTTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAat                                                                               >  1:771375/1‑235 (MQ=255)                                                                                                                                                                 aTTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGaaaaa                                                               >  1:354029/1‑251 (MQ=255)                                                                                                                                                                      aTTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATaaaa                                                                         <  1:249365/236‑1 (MQ=255)                                                                                                                                                                      aTTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCa                                                          >  1:607500/1‑251 (MQ=255)                                                                                                                                                                         gtgtgtAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCacc                                                        >  1:192275/1‑248 (MQ=255)                                                                                                                                                                         gtgtgtAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCacc                                                        >  1:545623/1‑248 (MQ=255)                                                                                                                                                                           gtgtAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAAt                                                                                                                                                                                    >  1:793074/1‑124 (MQ=255)                                                                                                                                                                           gtgtAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCaa                                                     >  1:722549/1‑251 (MQ=255)                                                                                                                                                                             gtAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGc                                                                                                                              >  1:480843/1‑176 (MQ=255)                                                                                                                                                                              tAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAAt                                                   >  1:353316/1‑250 (MQ=255)                                                                                                                                                                                   gAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAgg                                               >  1:179695/1‑249 (MQ=255)                                                                                                                                                                                        gACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCtttt                                          >  1:396207/1‑249 (MQ=255)                                                                                                                                                                                             ccGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCg                                   >  1:548456/1‑251 (MQ=255)                                                                                                                                                                                                     aCAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGa                            >  1:849291/1‑250 (MQ=255)                                                                                                                                                                                                             gTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGt                                                                                                                <  1:71927/158‑1 (MQ=255)                                                                                                                                                                                                                      aaTGGCAGCATGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCa          <  1:467023/251‑1 (MQ=255)                                                                                                                                                                                                                           cagcaTGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTGCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCAtata      <  1:228159/250‑1 (MQ=255)                                                                                                                                                                                                                             gcaTGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg    >  1:739742/1‑250 (MQ=255)                                                                                                                                                                                                                              caTGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg    <  1:734407/249‑1 (MQ=255)                                                                                                                                                                                                                              caTGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg    <  1:498474/249‑1 (MQ=255)                                                                                                                                                                                                                              caTGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg    <  1:456852/249‑1 (MQ=255)                                                                                                                                                                                                                              caTGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg    <  1:879467/249‑1 (MQ=255)                                                                                                                                                                                                                              caTGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg    <  1:428339/249‑1 (MQ=255)                                                                                                                                                                                                                              caTGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGaa  >  1:739821/1‑251 (MQ=255)                                                                                                                                                                                                                              caTGAAAGGCGCCGGTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAGaa  >  1:92976/1‑251 (MQ=255)                                                                                                                                                                                                                                            gTTTTCAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCACCCAAATCAGGCTTTTCTTGCCGACGCTGACGGTGCGCGGCATCACCATATAAg    >  1:684943/1‑235 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                        GGATTGGGCAATTTCCTGATCTAATTCATTGATGCGTTTTTGCATTATATCGTGGCAATATTCCGCCAGTTCGCGCACGTTTTCACGGGTATAGCCGGAAACGTCAATGGGTTCCAGCATTTCACAAATCACCTTGCCGTTGTTCCAGCGGTTTAAATCGATTTTATTGTGTGTAGTGGAGCAGACCACCGGCACGACAGGCACGTCGGCAGCAATGGCAGCATGAAAGGCGCCGGTTTTAAACGGCAATAATCCGCGTCCGCGACTGCGAGTGCCTTCCGGGAACATCCAAATAGACAGATTGTCCTGATTTATGCGGCGTGCCAGTTCCGTCATGGTGTTGTGGGCTTTGGAGCGATTGTCGCGATCCAGAAAAATATTGCCCGTCGCCCAATATAAAATGCCGAAAAACGGCAACCAAATCAGGCTTTTCTTACCGACGCTGACGGTGCGCGGCATCACCATATAAGAA  >  1/254097‑254568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑