Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,483,477	T→C	P142P (CCT→CCC)	unknown →	Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,483,477	0	T	C	100.0%	214.4 / NA	78	P142P (CCT→CCC)	unknown	Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/45);  total (33/45)

ATTGAGGGTTATGGACCATTACGAGATCTCATGGCTGACGATACTATAAATGATATTTTAGTTAATGGCCCTAATGACATCTGGGTTGAACGTGCTGGTATTCTGGAAAAAACTGATAAAGAATTTGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGCCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCTGATGGCAGCCGTTTAAATGTTGTTATTGCACCGATTGCATTAGATGGCACATCCATTTCTATTCGTAAATTCAGTAAGAATAAAAAAACATTGCAAGAGCTTGTAAATTTTGGTTCTATGACTCGCGAAATGGCGAATTTCTTAATTATTGCTGCCAGATCCCGGGTAAATATAATTGTGTCAGGTGGTACAGGATCTGGTAAAACAACCTTACTTAATGCGCTATCTAACTATATTTCCCATAC  >  1/1483253‑1483722                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      aTTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAAtgttgtaat                                                                                                                                                                                                                             <  1:188624/251‑4 (MQ=255)      gggTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGc                                                                                                                                                                                                                                              <  1:594915/229‑1 (MQ=255)      gggTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAAt                                                                                                                                                                                                                                     <  1:329677/238‑1 (MQ=255)       ggTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCAc                                                                                                                                                                                                                        >  1:698670/1‑250 (MQ=255)        gTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCg                                                                                                                                                                                                                      >  1:698551/1‑251 (MQ=255)              gtccgttACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGcg                                                                                                                                                                                                                >  1:41424/6‑250 (MQ=255)                    tACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGAt                                                                                                                                                                                                          <  1:823350/251‑1 (MQ=255)                    tACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGAt                                                                                                                                                                                                          <  1:705393/251‑1 (MQ=255)                              cgtGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCa                                                                                                                                                                                                <  1:863456/249‑1 (MQ=255)                                       cGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGAt                                                                                                                                                                                                          <  1:780385/232‑1 (MQ=255)                                          tACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTa                                                                                                                                                                                    <  1:834489/251‑1 (MQ=255)                                            cTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTaag                                                                                                                                                                                  >  1:366335/1‑250 (MQ=255)                                            cTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTaag                                                                                                                                                                                  >  1:852340/1‑250 (MQ=255)                                                aaaTGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCa                                                                                                                                                                              >  1:182602/1‑251 (MQ=255)                                                    gATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGTTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAAtg                                                                                                                                                                                                                                    >  1:37101/1‑193 (MQ=255)                                                     atatCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTaagt                                                                                                                                                                                 >  1:46413/1‑241 (MQ=255)                                                       atcttAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGag                                                                                                                                                                       >  1:258190/4‑250 (MQ=255)                                                             gTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTc                                                                                                                                                                               >  1:649862/1‑237 (MQ=255)                                                              ttAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTaaaaaa                                                                                                                                                                >  1:247948/1‑251 (MQ=255)                                                              ttAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTaaaaaa                                                                                                                                                                <  1:286497/251‑1 (MQ=255)                                                              ttAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTaaaaaa                                                                                                                                                                <  1:834553/251‑1 (MQ=255)                                                              ttAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTaaaaa                                                                                                                                                                 <  1:170150/250‑1 (MQ=255)                                                                    gCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAAtgttgtaat                                                                                                                                                                                                                             <  1:644040/184‑4 (MQ=255)                                                                    gCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATggtac                                                                                                                                                                                                     <  1:6628/208‑4 (MQ=255)                                                                     cccTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTaaaaaaagcttg                                                                                                                                                          >  1:813685/1‑245 (MQ=255)                                                                        tAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAag                                                                                                                                                      <  1:136815/251‑1 (MQ=255)                                                                         aaTGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCa                                                                                                                                                                                                >  1:534121/1‑208 (MQ=255)                                                                              taTCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAAtg                                                                                                                                                                                                                                    >  1:415185/2‑167 (MQ=255)                                                                              taTCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCAc                                                                                                                                                                                                                        <  1:53735/178‑1 (MQ=255)                                                                              taTCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAag                                                                                                                                                      <  1:557463/244‑1 (MQ=255)                                                                              taTCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTg                                                                                                                                                >  1:818981/2‑251 (MQ=255)                                                                                   gggTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTc                                                                                                                                                                                            >  1:460207/1‑202 (MQ=255)                                                                                    ggTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTc                                                                                                                                                                               <  1:751771/214‑1 (MQ=255)                                                                                                                         gAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCAc                                                                                                                                                                                                                        <  1:137201/136‑1 (MQ=255)                                                                                                                              cgTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAattatcg                                                                                                >  1:374876/2‑249 (MQ=255)                                                                                                                                   gTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATggtac                                                                                                                                                                                                     >  1:133722/1‑142 (MQ=255)                                                                                                                                   gTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATcgctgc                                                                                            >  1:445950/1‑250 (MQ=255)                                                                                                                                          aCAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATct                                                                                     >  1:538625/1‑249 (MQ=255)                                                                                                                                           cAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATg                                                                                                                                                                                                         >  1:322869/1‑133 (MQ=255)                                                                                                                                           cAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTaaaaa                                                                                                                                                                 <  1:53035/173‑1 (MQ=255)                                                                                                                                             aTTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATctcgag                                                                                 <  1:593720/251‑6 (MQ=255)                                                                                                                                             aTTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATctcgag                                                                                 >  1:104960/1‑246 (MQ=255)                                                                                                                                                aaCTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAAtt                                                                                                                                          >  1:603678/1‑191 (MQ=255)                                                                                                                                                aaCTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGcc                                                                                           <  1:105483/238‑1 (MQ=255)                                                                                                                                                aaCTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTaa                                                                              >  1:150133/1‑251 (MQ=255)                                                                                                                                                       atTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAAt                                                                       <  1:422609/251‑1 (MQ=255)                                                                                                                                                         tGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATtg                                                                     <  1:594926/251‑1 (MQ=255)                                                                                                                                                         tGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATtg                                                                     <  1:211482/251‑1 (MQ=255)                                                                                                                                                          gCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATtgt                                                                    >  1:501982/1‑251 (MQ=255)                                                                                                                                                            aaaaaGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTgtgt                                                                  <  1:457632/251‑1 (MQ=255)                                                                                                                                                                  gTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAggt                                                             <  1:597092/250‑1 (MQ=255)                                                                                                                                                                  gTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAggt                                                             <  1:171629/250‑1 (MQ=255)                                                                                                                                                                        tGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAg                                                      <  1:550899/251‑1 (MQ=255)                                                                                                                                                                        tGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAg                                                      <  1:401498/251‑1 (MQ=255)                                                                                                                                                                           tCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGAt                                                   <  1:854231/251‑1 (MQ=255)                                                                                                                                                                           tCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGAt                                                   <  1:17210/251‑1 (MQ=255)                                                                                                                                                                              ggTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAatat                                                                          <  1:132605/225‑1 (MQ=255)                                                                                                                                                                                     cgtcGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAaac                                         <  1:618678/248‑1 (MQ=255)                                                                                                                                                                                     cgtcGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAaac                                         <  1:180538/248‑1 (MQ=255)                                                                                                                                                                                       tcGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGAt                                                                                       <  1:898626/202‑1 (MQ=255)                                                                                                                                                                                       tcGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAaacaa                                       >  1:323857/2‑251 (MQ=255)                                                                                                                                                                                         gTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAaacaa                                       >  1:273647/1‑249 (MQ=255)                                                                                                                                                                                              gatgatGGTAGTCCACTAGTGGACTCCCGACTTCCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATggtac                                                                                                                                                                                                     <  1:566757/86‑4 (MQ=255)                                                                                                                                                                                                tgatgGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGAt                                                   <  1:272976/230‑1 (MQ=255)                                                                                                                                                                                                  atgGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAg                                                                                                                                                                         >  1:893709/1‑110 (MQ=255)                                                                                                                                                                                                   tgGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAaacaactctccttaatg                           >  1:519038/1‑240 (MQ=255)                                                                                                                                                                                                   tgGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAaacaactctccttaatg                           <  1:819003/251‑12 (MQ=255)                                                                                                                                                                                                   tgGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAaacaactctccttaatg                           <  1:814888/251‑12 (MQ=255)                                                                                                                                                                                                     gTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATgc                          <  1:635676/250‑1 (MQ=255)                                                                                                                                                                                                        gTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTa                      >  1:40588/1‑251 (MQ=255)                                                                                                                                                                                                        gTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTa                      >  1:601913/1‑251 (MQ=255)                                                                                                                                                                                                                gTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTaatta              >  1:408684/1‑248 (MQ=255)                                                                                                                                                                                                                 tGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTTGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTaatta              <  1:448071/250‑4 (MQ=255)                                                                                                                                                                                                                 tGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTaatta              <  1:422294/250‑4 (MQ=255)                                                                                                                                                                                                                 tGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTaatta              <  1:697782/250‑4 (MQ=255)                                                                                                                                                                                                                   gACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATtata            <  1:2897/250‑1 (MQ=255)                                                                                                                                                                                                                          gACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCAt    >  1:249121/1‑251 (MQ=255)                                                                                                                                                                                                                            cTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTGTAAATTTCGGTTCTATGACCCGCGATATGGCAAATTTTTTAATTATCGCTGCCAGATCTCGAGTAAATATAATTGTGTCAGGTGGTACAGGATCCGGTAAAACAACTCTCCTTAATGCGCTATCTAATTATATTTCCCATAc  <  1:235047/251‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      ATTGAGGGTTATGGACCATTACGAGATCTCATGGCTGACGATACTATAAATGATATTTTAGTTAATGGCCCTAATGACATCTGGGTTGAACGTGCTGGTATTCTGGAAAAAACTGATAAAGAATTTGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGCCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCTGATGGCAGCCGTTTAAATGTTGTTATTGCACCGATTGCATTAGATGGCACATCCATTTCTATTCGTAAATTCAGTAAGAATAAAAAAACATTGCAAGAGCTTGTAAATTTTGGTTCTATGACTCGCGAAATGGCGAATTTCTTAATTATTGCTGCCAGATCCCGGGTAAATATAATTGTGTCAGGTGGTACAGGATCTGGTAAAACAACCTTACTTAATGCGCTATCTAACTATATTTCCCATAC  >  1/1483253‑1483722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑