Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,483,378	T→C	F109F (TTT→TTC)	unknown →	Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,483,378	0	T	C	100.0%	162.5 / NA	59	F109F (TTT→TTC)	unknown	Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/28);  total (31/28)

GCTCACTGGTAAACGAACTGGTTCAGATTGTTAACCGTGTTGCCAATAAAAGCGGTGCTTATTTGACCTCAGCCGATACTCTAGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGACCATTACGAGATCTCATGGCTGACGATACTATAAATGATATTTTAGTTAATGGCCCTAATGACATCTGGGTTGAACGTGCTGGTATTCTGGAAAAAACTGATAAAGAATTTGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGCCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCTGATGGCAGCCGTTTAAATGTTGTTATTGCACCGATTGCATTAGATGGCACATCCATTTCTATTCGTAAATTCAGTAAGAATAAAAAAACATTGCAAGAGCTTG  >  1/1483143‑1483580                                                                                                                                                                                                                                            |                                                                                                                                                                                                           gctcattagTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAAc                                                                                                                                                                                               >  1:378392/9‑249 (MQ=255)      ttagTAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTaa                                                                                                                                                                                         >  1:719901/4‑250 (MQ=255)          tAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAGTTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACAGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGa                                                                                                                                                                                     <  1:91403/250‑1 (MQ=255)          tAAACGAACTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGat                                                                                                                                                                                    >  1:203429/1‑251 (MQ=255)                aaCTGGTTCAGATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCa                                                                                                                                                                              >  1:865880/1‑251 (MQ=255)                          gATTGTTAATCGTATTGCCAATAAAAGTAACACTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACt                                                                                                                                                                                       <  1:338096/232‑1 (MQ=255)                                             aataaaagtaacacTTATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCgtcg                                                                                                                                                 >  1:15157/14‑248 (MQ=255)                                                            tATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATtgatg                                                                                                                                         >  1:835611/1‑244 (MQ=255)                                                            tATTTGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATtgatg                                                                                                                                         >  1:287448/1‑244 (MQ=255)                                                              tttGACGTCATCCGATGCACTGGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTc                                                                                                                                <  1:865849/251‑1 (MQ=255)                                                                                   ggTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGAc                                                                                                                     <  1:845249/240‑1 (MQ=255)                                                                                   ggTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTa                                                                                                            >  1:521936/2‑250 (MQ=255)                                                                                     ttATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTAccc                                                                                                         <  1:758068/251‑2 (MQ=255)                                                                                                 ttgttgCTGATGAGATTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATtgatg                                                                                                                                         >  1:353882/1‑207 (MQ=255)                                                                                                              gaTTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATtgatg                                                                                                                                         >  1:742115/1‑194 (MQ=255)                                                                                                               aTTGAGGGTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAAtgttgtaat                                                                               <  1:188624/251‑4 (MQ=255)                                                                                                                    gggTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGc                                                                                                <  1:594915/229‑1 (MQ=255)                                                                                                                    gggTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAAt                                                                                       <  1:329677/238‑1 (MQ=255)                                                                                                                     ggTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCAc                                                                          >  1:698670/1‑250 (MQ=255)                                                                                                                      gTTATGGTCCGTTACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCg                                                                        >  1:698551/1‑251 (MQ=255)                                                                                                                            gtccgttACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGcg                                                                  >  1:41424/6‑250 (MQ=255)                                                                                                                                  tACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGAt                                                            <  1:823350/251‑1 (MQ=255)                                                                                                                                  tACGGGATCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGAt                                                            <  1:705393/251‑1 (MQ=255)                                                                                                                                        aTCTCGTGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGAc                                                                                                                     <  1:630038/188‑1 (MQ=255)                                                                                                                                            cgtGGCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCa                                                  <  1:863456/249‑1 (MQ=255)                                                                                                                                                gCTGACGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACt                                                                                                             <  1:42072/188‑1 (MQ=255)                                                                                                                                                     cGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACt                                                                                                                                                                                       <  1:754147/109‑1 (MQ=255)                                                                                                                                                     cGATACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGAt                                                            <  1:780385/232‑1 (MQ=255)                                                                                                                                                       aTACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCAc                                                                                                                             >  1:113331/1‑165 (MQ=255)                                                                                                                                                        tACTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTa                                      <  1:834489/251‑1 (MQ=255)                                                                                                                                                          cTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATtg                                                                                                                                            <  1:453546/147‑1 (MQ=255)                                                                                                                                                          cTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTaag                                    >  1:852340/1‑250 (MQ=255)                                                                                                                                                          cTATAAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTaag                                    >  1:366335/1‑250 (MQ=255)                                                                                                                                                            ataAATGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAg                                                                                                                          >  1:63955/1‑163 (MQ=255)                                                                                                                                                              aaaTGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCa                                >  1:182602/1‑251 (MQ=255)                                                                                                                                                               aaTGATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGAc                                                                                                                     <  1:832628/165‑1 (MQ=255)                                                                                                                                                                  gATATCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGTTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAAtg                                                                                      >  1:37101/1‑193 (MQ=255)                                                                                                                                                                   atatCTTAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTaagt                                   >  1:46413/1‑241 (MQ=255)                                                                                                                                                                     atcttAGTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGag                         >  1:258190/4‑250 (MQ=255)                                                                                                                                                                           gTTAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTc                                 >  1:649862/1‑237 (MQ=255)                                                                                                                                                                            ttAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTaaaaaa                  <  1:286497/251‑1 (MQ=255)                                                                                                                                                                            ttAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTaaaaaa                  <  1:834553/251‑1 (MQ=255)                                                                                                                                                                            ttAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTaaaaaa                  >  1:247948/1‑251 (MQ=255)                                                                                                                                                                            ttAATGGCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTaaaaa                   <  1:170150/250‑1 (MQ=255)                                                                                                                                                                                  gCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAAtgttgtaat                                                                               <  1:644040/184‑4 (MQ=255)                                                                                                                                                                                  gCCCTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATggtac                                                       <  1:6628/208‑4 (MQ=255)                                                                                                                                                                                   cccTAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTaaaaaaagcttg            >  1:813685/1‑245 (MQ=255)                                                                                                                                                                                      tAATGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAag        <  1:136815/251‑1 (MQ=255)                                                                                                                                                                                       aaTGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAg                                                                                                                          >  1:520387/1‑136 (MQ=255)                                                                                                                                                                                       aaTGATATCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCa                                                  >  1:534121/1‑208 (MQ=255)                                                                                                                                                                                            taTCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGat                                                                                                                                                                                    >  1:176699/2‑73 (MQ=255)                                                                                                                                                                                            taTCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAAtg                                                                                      >  1:415185/2‑167 (MQ=255)                                                                                                                                                                                            taTCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCAc                                                                          <  1:53735/178‑1 (MQ=255)                                                                                                                                                                                            taTCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAag        <  1:557463/244‑1 (MQ=255)                                                                                                                                                                                            taTCTGGGTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTCAGTAAGAGTAAAAAAAGCTTGCAAGAGCTTg  >  1:818981/2‑251 (MQ=255)                                                                                                                                                                                                 gggTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTc                                              >  1:460207/1‑202 (MQ=255)                                                                                                                                                                                                  ggTTGAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCACCGATTGCGTTAGATGGTACATCCATTTCCATTCGTAAGTTc                                 <  1:751771/214‑1 (MQ=255)                                                                                                                                                                                                      gAACGTGCCGGTATTCTGGAAAAAACTGATAAAGAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTAcc                                                                                                          >  1:80993/1‑137 (MQ=255)                                                                                                                                                                                                                                       gAATTCGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGTCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCCGATGGTAGCCGTTTAAATGTTGTAATTGCAc                                                                          <  1:137201/136‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                           GCTCACTGGTAAACGAACTGGTTCAGATTGTTAACCGTGTTGCCAATAAAAGCGGTGCTTATTTGACCTCAGCCGATACTCTAGTTATGGCTGAAATTGTTGCTGATGAGATTGAGGGTTATGGACCATTACGAGATCTCATGGCTGACGATACTATAAATGATATTTTAGTTAATGGCCCTAATGACATCTGGGTTGAACGTGCTGGTATTCTGGAAAAAACTGATAAAGAATTTGTTAGTAATGAACAATTAACTGATATTGCAAAAAGGTTAGTTGCTCGGGTTGGTCGCCGTATTGATGATGGTAGTCCACTAGTGGACTCCCGACTACCTGATGGCAGCCGTTTAAATGTTGTTATTGCACCGATTGCATTAGATGGCACATCCATTTCTATTCGTAAATTCAGTAAGAATAAAAAAACATTGCAAGAGCTTG  >  1/1483143‑1483580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑